Prupe.8G039600_v2.0.a1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0015060
Physical Location & Seq
Reverse (-)
3962763 .. 3966543
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G039600.1

Sequence Viewer

Length: 999 bp
ATGGCGTCGTCTTCTTCTTCTTCGTCATCAGCCTACGACATACCATGGGTGGAGAAGTACAGGCCCAGCAATGTCGCCGACATTGTGGGCAACGAAGACGCCGTGTCTAGGCTCCAGGTCATCGCCCGCGACGGCAACATGCCCAACCTCATCTTGGCCGGTCCTCCTGGAACTGGTAAGACCACTAGTATACTCGCTCTTGCGCATGAGCTCTTGGGACCAAATTATAAGGAGGCTGTTTTAGAGCTGAATGCATCAGACGACAGGGGAATAGATGTTGTGAGGAACAAAATTAAAATGTTTGCTCAAAAGAAAGTCACATTGCCTCCTGGGCGGCATAAAGTTGTCATCTTGGATGAAGCTGACAGTATGACATCTGGAGCACAACAAGCTTTGAGGCGGACAATGGAAATATATTCAAATTCCACTCGTTTTGCTCTTGCGTGCAATGTATCTTCCAAAATTATTGAGCCTATTCAGAGTAGATGTGCCCTTGTCCGATTTTCTAGATTATCTGATCAGGAGATACTTGGTCGCCTAATGATAGTGGTTCAAGCTGAAAAGGTTCCTTATGTTCCAGAAGGTCTTGAAGCCATTATTTTCACTGCTGATGGTGATATGAGGCAGGCACTGAATAATCTGCAAGCTACAAACAGTGGATTCCGGTTTGTCAACCAAGAAAATGTTTTCAAGGTCTGTGACCAGCCTCATCCATTGCATGTAAAGAATATGGTGTGCAATGTGCTTGAAGGGAAATTTGATGATGCTTGTTCAGGTCTCAAGCAGCTCTATGATTTGGGCTACTCCCCAACTGACATAATCACTACCTTTTTCCGTATCATAAAGAATTATGATATGGCTGAGTATTTGAAATTGGAATTCATGAAAGAAACAGGTTTTGCTCACATGAGAATCTGTGATGGAGTTGGCTCCTACCTTCAGCTGTGTGGTCTTCTGGCTAAGCTTTCCCTAATTCGTGAGACAGCTAAAGCGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000723 GO:0000731 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0030554 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031390 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033260 GO:0033554 GO:0033683 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0036094 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097367 GO:1900262 GO:1900264 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000112 GO:2000278 GO:2000573
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA Rep_fac_C

Protein Analysis

333

Amino Acids

36.69

Weight (kDa)

6.95

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63160
fragaria_vesca FvH4_5g01730 FvH4_5g01730
malus_domestica MD04G1065200.v1.1
prunus_persica Prupe.8G039600_v2.0.a1
pyrus_communis pycom04g05880
rosa_chinensis RchiOBHm_Chr7g0198991
rosa_laevigata RLG00000003826
rosa_multiflora Rmu_sc0006266.1_g000025 Rmu_sc0007328.1_g000009
rosa_roxburghii Rroxscaffold_3G00257230
rosa_rugosa Rorug07G0052000
rosa_samantha Rh7AG175000 Rh7BG178200 Rh7DG177600
rosa_wichuraiana Rw7G015350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
Acc16I TGCGCA 1 cut(s) 204
AccI GTMKAC 1 cut(s) 190
AccII CGCG 1 cut(s) 129
AciI CCGC 3 cut(s) 127, 334, 400
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 3 cut(s) 421, 755, 878
AcuI CTGAAG 1 cut(s) 923
AcyI GRCGYC 2 cut(s) 5, 99
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 3 cut(s) 108, 154, 173
AgsI TTSAA 6 cut(s) 420, 554, 590, 691, 749, 871
AhdI GACNNNNNGTC 1 cut(s) 103
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 3 cut(s) 114, 166, 328
Alw21I GWGCWC 2 cut(s) 213, 385
Alw26I GTCTC 2 cut(s) 782, 974
AlwNI CAGNNNCTG 1 cut(s) 631
AoxI GGCC 2 cut(s) 62, 156
ApeKI GCWGC 1 cut(s) 784
ApoI RAATTY 3 cut(s) 421, 755, 878
Asp700I GAANNNNTTC 1 cut(s) 564
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 3 cut(s) 63, 161, 218
AsuHPI GGTGA 1 cut(s) 626
AvaII GGWCC 2 cut(s) 161, 218
BaeGI GKGCMC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 3 cut(s) 3, 102, 944
Bbv12I GWGCWC 2 cut(s) 213, 385
BbvI GCAGC 1 cut(s) 796
BccI CCATC 2 cut(s) 605, 914
BceAI ACGGC 2 cut(s) 86, 148
BciT130I CCWGG 3 cut(s) 116, 168, 330
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 782, 974
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 3 cut(s) 108, 186, 507
BglI GCCNNNNNGGC 1 cut(s) 331
BisI GCNGC 2 cut(s) 335, 785
BlpI GCTNAGC 1 cut(s) 960
BlsI GCNGC 2 cut(s) 336, 786
Bme1390I CCNGG 3 cut(s) 116, 168, 330
Bme18I GGWCC 2 cut(s) 161, 218
BmeRI GACNNNNNGTC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 63, 161, 218
BmiI GGNNCC 4 cut(s) 113, 219, 567, 931
BmrFI CCNGG 3 cut(s) 116, 168, 330
BmsI GCATC 2 cut(s) 263, 754
BpiI GAAGAC 3 cut(s) 3, 102, 944
BpmI CTGGAG 2 cut(s) 98, 399
Bpu1102I GCTNAGC 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 764
BsaHI GRCGYC 2 cut(s) 5, 99
BsaI GGTCTC 1 cut(s) 782
BsaJI CCNNGG 2 cut(s) 44, 329
BsaWI WCCGGW 1 cut(s) 663
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 154, 173
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 178
Bse3DI GCAATG 5 cut(s) 76, 320, 454, 713, 745
BseBI CCWGG 3 cut(s) 116, 168, 330
BseDI CCNNGG 2 cut(s) 44, 329
BseGI GGATG 2 cut(s) 361, 709
BseLI CCNNNNNNNGG 3 cut(s) 108, 154, 173
BseMI GCAATG 5 cut(s) 76, 320, 454, 713, 745
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 1 cut(s) 178
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 1 cut(s) 796
BseYI CCCAGC 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 64, 158
BsiHKAI GWGCWC 2 cut(s) 213, 385
BsiSI CCGG 2 cut(s) 159, 664
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 3 cut(s) 108, 154, 173
BsmAI GTCTC 2 cut(s) 782, 974
BsmFI GGGAC 1 cut(s) 231
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 2 cut(s) 64, 158
Bso31I GGTCTC 1 cut(s) 782
Bsp1286I GDGCHC 3 cut(s) 213, 385, 493
Bsp143I GATC 1 cut(s) 517
Bsp1720I GCTNAGC 1 cut(s) 960
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 3 cut(s) 127, 334, 400
BspANI GGCC 2 cut(s) 64, 158
BspCNI CTCAG 1 cut(s) 853
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 4 cut(s) 113, 219, 567, 931
BspTNI GGTCTC 1 cut(s) 782
BsrDI GCAATG 5 cut(s) 76, 320, 454, 713, 745
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 44, 329
BssMI GATC 1 cut(s) 517
BssNAI GTATAC 1 cut(s) 191
BssNI GRCGYC 2 cut(s) 5, 99
BssT1I CCWWGG 1 cut(s) 44
Bst1107I GTATAC 1 cut(s) 191
Bst2UI CCWGG 3 cut(s) 116, 168, 330
Bst4CI ACNGT 2 cut(s) 368, 656
BstACI GRCGYC 2 cut(s) 5, 99
BstC8I GCNNGC 4 cut(s) 127, 445, 627, 645
BstDEI CTNAG 2 cut(s) 861, 960
BstDSI CCRYGG 1 cut(s) 44
BstF5I GGATG 2 cut(s) 361, 709
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 2 cut(s) 782, 974
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 2 cut(s) 331, 389
BstNI CCWGG 3 cut(s) 116, 168, 330
BstNSI RCATGY 2 cut(s) 142, 722
BstSCI CCNGG 3 cut(s) 114, 166, 328
BstSLI GKGCMC 1 cut(s) 493
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 796
BstV2I GAAGAC 3 cut(s) 3, 102, 944
BstZ17I GTATAC 1 cut(s) 191
BsuRI GGCC 2 cut(s) 64, 158
BtgI CCRYGG 1 cut(s) 44
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 2 cut(s) 361, 709
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 603, 629, 661
Cac8I GCNNGC 4 cut(s) 127, 445, 627, 645
CaiI CAGNNNCTG 1 cut(s) 631
CciI TCATGA 1 cut(s) 882
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 63, 161, 218
CseI GACGC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 58
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 6 cut(s) 45, 139, 206, 719, 883, 907
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 861, 960
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
DriI GACNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 156
Eam1105I GACNNNNNGTC 1 cut(s) 103
EciI GGCGGA 1 cut(s) 415
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 782
Eco47I GGWCC 2 cut(s) 161, 218
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 923
EcoICRI GAGCTC 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 3 cut(s) 114, 166, 328
EcoT14I CCWWGG 1 cut(s) 44
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 6 cut(s) 48, 142, 209, 722, 886, 910
FaqI GGGAC 1 cut(s) 231
FatI CATG 6 cut(s) 44, 138, 205, 718, 882, 906
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 335, 785
FokI GGATG 2 cut(s) 368, 696
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 2 cut(s) 335, 785
FspBI CTAG 3 cut(s) 108, 186, 507
FspI TGCGCA 1 cut(s) 204
GlaI GCGC 1 cut(s) 204
GluI GCNGC 2 cut(s) 335, 785
GsaI CCCAGC 1 cut(s) 69
GsuI CTGGAG 2 cut(s) 98, 399
HaeIII GGCC 2 cut(s) 64, 158
HapII CCGG 2 cut(s) 159, 664
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 2 cut(s) 5, 99
Hin1II CATG 6 cut(s) 48, 142, 209, 722, 886, 910
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 2 cut(s) 390, 962
HinfI GANTC 2 cut(s) 660, 912
HpaII CCGG 2 cut(s) 159, 664
HphI GGTGA 1 cut(s) 626
Hpy166II GTNNAC 2 cut(s) 191, 673
Hpy188I TCNGA 4 cut(s) 259, 480, 500, 517
Hpy188III TCNNGA 7 cut(s) 378, 507, 521, 578, 587, 883, 977
Hpy8I GTNNAC 2 cut(s) 191, 673
Hpy99I CGWCG 2 cut(s) 10, 134
HpyAV CCTTC 3 cut(s) 575, 743, 947
HpyCH4III ACNGT 2 cut(s) 368, 656
HpyCH4V TGCA 5 cut(s) 254, 447, 643, 718, 738
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 389
HpyF3I CTNAG 2 cut(s) 861, 960
Hsp92I GRCGYC 2 cut(s) 5, 99
Hsp92II CATG 6 cut(s) 48, 142, 209, 722, 886, 910
HspAI GCGC 1 cut(s) 203
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 3 cut(s) 117, 380, 935
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 2 cut(s) 263, 754
MaeI CTAG 3 cut(s) 108, 186, 507
MaeIII GTNAC 2 cut(s) 316, 698
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 7 cut(s) 3, 6, 9, 12, 107, 447, 944
MhlI GDGCHC 3 cut(s) 213, 385, 493
MluCI AATT 9 cut(s) 223, 291, 421, 462, 755, 847, 872, 878, 972
MnlI CCTC 8 cut(s) 158, 174, 226, 276, 336, 390, 615, 717
Mph1103I ATGCAT 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 564
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 943
MspI CCGG 2 cut(s) 159, 664
MspR9I CCNGG 3 cut(s) 116, 168, 330
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 3 cut(s) 116, 168, 330
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 331, 389
NcoI CCATGG 1 cut(s) 44
NdeII GATC 1 cut(s) 517
NlaIII CATG 6 cut(s) 48, 142, 209, 722, 886, 910
NlaIV GGNNCC 4 cut(s) 113, 219, 567, 931
NmuCI GTSAC 2 cut(s) 316, 698
NsbI TGCGCA 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 2 cut(s) 142, 722
PagI TCATGA 1 cut(s) 882
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 2 cut(s) 660, 912
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 2 cut(s) 336, 786
PsiI TTATAA 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 3 cut(s) 114, 166, 328
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 3 cut(s) 114, 166, 328
PspN4I GGNNCC 4 cut(s) 113, 219, 567, 931
PspPI GGNCC 3 cut(s) 63, 161, 218
PstNI CAGNNNCTG 1 cut(s) 631
PvuII CAGCTG 1 cut(s) 943
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 2 cut(s) 335, 785
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 3 cut(s) 63, 161, 218
ScrFI CCNGG 3 cut(s) 116, 168, 330
SduI GDGCHC 3 cut(s) 213, 385, 493
SfaNI GCATC 2 cut(s) 263, 754
SinI GGWCC 2 cut(s) 161, 218
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 9 cut(s) 223, 291, 421, 462, 755, 847, 872, 878, 972
SsiI CCGC 3 cut(s) 127, 334, 400
SspMI CTAG 3 cut(s) 108, 186, 507
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 114, 166, 328
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 2 cut(s) 368, 656
TasI AATT 9 cut(s) 223, 291, 421, 462, 755, 847, 872, 878, 972
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 2 cut(s) 660, 912
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 3 cut(s) 610, 636, 661
TseFI GTSAC 2 cut(s) 316, 698
TseI GCWGC 1 cut(s) 784
Tsp45I GTSAC 2 cut(s) 316, 698
TspDTI ATGAA 3 cut(s) 372, 871, 899
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 3 cut(s) 610, 636, 661
VpaK11BI GGWCC 2 cut(s) 161, 218
XapI RAATTY 3 cut(s) 421, 755, 878
XbaI TCTAGA 1 cut(s) 506
XceI RCATGY 2 cut(s) 142, 722
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 3 cut(s) 108, 186, 507
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.