Rorug07G0052000

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015060
Physical Location & Seq
Reverse (-)
3720642 .. 3724785
4144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0052000.1

Sequence Viewer

Length: 2373 bp
ATGGAGGCACAACCATCTTCGCTATCACCGTCATCTTCATCATCTTGCAGTACTAATGATAACATTATGCCGCTTTCTTTCTCTCCAGTTTGGACTGAATCCGATAAAACAGAACCACTGCAAGCTGTGAACCAGGAGAAGGCATCCACTCCCAGGTCAGATACTGAATTGCTGAGATTTATGGACCTCACTGCCAATGGTGGGATGAGGTGGGAGGATGTAGAGGACCGGTTTGACCGGCTTGCCATCACACATTGCCAAATTGATCCAGTTATAAAATGGTCTGATTTTGGTTTGTGCATAGGGATGAAACAGTCGCAAGAGTTTGCTAATCAGCTATTAAGGGCTCTAAGGAAAAGCAAGGATTGCAATTTTTGTATAAGAAAAGCTGAACTGCACAGTTACTGGTGTCGTATGACTGACCCTTGCTTCAATTCAAGGATGCAGATTTTCTTTGACTTGTGTGACAGAAACATGGAAGGAAGAATGACTGAGATCGATATTAAGCAGACTATTATACTAAGTGCTACTACAAATAAGTTATTTTTGACACCTGAGGAAGCAGAAGACTATGCTGCTTTGGTCATGGAGCTGCTTGACACTGAAAAAAGAGGCTACATTGAGCTTCCCCAATTTGAGACTCTTTTTAAAGTGAGCTTATCAAAGGCCTCCTTCTCAAATAACCACTTGTCAATCAGACATGATCCGTATCACAATTTTGACCGAGTTCAGGAGCCAAATTCCAGGATAGAAATTTTGTTTCGATCTTATTGGCGACGAGCATGGATAGTTTTGTTGTGGTTGACCATATGTAGTGCACTGTTCACTTGGAAATTTCTGCAATATAGGCATAGAGCAGCTTTTCAAGTAATGGGGTACTGCCTATCCTCTGCCAAAGGTGCTGCTGAAACCCTTAAATTCAACATGGCTCTAATTCTCCTCCCTGTATGTCGTAATACAATTACATGGCTCCGAAGGAATCGTTCAGTCAACTCGATCATTCCATTCAATGACACTATCAACTTCCACAAGCTCATCGCAGGAGGGATAGTAGTAGGAGTTATCCTACATGGTGGTTGTCACCTAGCCTGTGATTTACCAAGAATTAGTGATTCAGATCATGTAATCTTCAGGCAAACAATAGCTGCCCAATTCGGGTACCATCAACCATCATACTTTGAAATATTGTCCACAAAGGAAGTGGCATCAGGGATTGGTACGGTAATACTAATGGCCATAGCATTCTCACTTGCAACAACATGGCCACGCCGGGGGTCACCTTCACTGCCTAAGTCTGTCCGGAATGTCACAGGATACAATACCTTTTGGTACTCACACCACTTGTTCATACCTGTATATGCATTGCTCATTTTTCACTCTATATTCCTCTTCCTTACAAACAACGTAAATGAAAAAACGACATGGATGTACATTGCCATTCCAGTTCTGTTATATACTGGAGAACGAGTATTTCGAGCCATGAGATCAGGATTTTATGAGGTGGAAATGTTGAAGGCAAGTATTCATCCTGGAAAAGTTGTGTCCCTCAAATTGCACAAACCAAAAGGTTTCAAGTACCTGAGTGGCATGTACATATTCATCCAATGCCCTCAGATTTCACCATTTGAATGGCACCCGTTTTCGTTAACATCAGGACCAGAAGATGACTACCTAAGTGTGCATATTAGAACTTCTGGGGACTGGAGTTACCAAATATACAGTCTTTTCCAAGAGGCAACATTATCTGGACTTAACAGTTACCCCAAAGTACACATTGATGGGCCTTATGGTGCTGCTTCCCAAGACCATGTCAAGTATGATATCATTGTTCTAATCGGTCTAGGCATAGGCGCTACACCTTTCATTGGCATCCTCAAAGATATTGTCAATGGTGCTCAAAACTCATCACATTGTGATCATCATTCTGGTTGCAGAGAATGCAGCTCAAGAAAGTCTCCACTAAAAGCCTATCTTTTTTGGGTCACAAGGGAACAAAGCTCCTTTGAATGGTTTAGAGACATCATGAAGGAAATATCAAACTCAAGCGAAAAGCAGTCTGTTGTGGAGATGCACAATTTCTTAACTTCTGTGTATCAAGAGGGAGATGCGCGCTCAGTTCTAATAAGTGCAATACAAGCATTGCATTATGCCAAAAATGGGATTGACTTTGTTTCCCGGACTCCGGTAAGAACACATTTTGCCCGGCCAAACTGGTCCAGCATCTTCTCAAAATTGGCATGTAAACACAGAAGAGCACGGATTGGGGTTTTCTACTGTGGCGCATCAACCTTAGCAAACGAGTTGGAGGGAATGTGTACCAAATTTTCGACCAAAACTTCCACGAGATTTGTATTTCACAAGGAGAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000723 GO:0000731 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0030554 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031390 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033260 GO:0033554 GO:0033683 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0036094 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097367 GO:1900262 GO:1900264 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000112 GO:2000278 GO:2000573
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA Rep_fac_C

Protein Analysis

790

Amino Acids

90.32

Weight (kDa)

8.71

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 57 - 146 2.9e-19 Respiratory burst NADPH oxidase
Ferric_reduct PF01794 309 - 456 5.1e-09 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 499 - 600 1.5e-27 FAD-binding domain
NAD_binding_6 PF08030 606 - 772 2.2e-39 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63160
fragaria_vesca FvH4_5g01730 FvH4_5g01730
malus_domestica MD04G1065200.v1.1
prunus_persica Prupe.8G039600_v2.0.a1
pyrus_communis pycom04g05880
rosa_chinensis RchiOBHm_Chr7g0198991
rosa_laevigata RLG00000003826
rosa_multiflora Rmu_sc0006266.1_g000025 Rmu_sc0007328.1_g000009
rosa_roxburghii Rroxscaffold_3G00257230
rosa_rugosa Rorug07G0052000
rosa_samantha Rh7AG175000 Rh7BG178200 Rh7DG177600
rosa_wichuraiana Rw7G015350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 1158
AccB1I GGYRCC 2 cut(s) 1158, 1632
AccII CGCG 1 cut(s) 2110
AccIII TCCGGA 1 cut(s) 1299
AciI CCGC 1 cut(s) 71
AclWI GGATC 2 cut(s) 260, 698
AcoI YGGCCR 3 cut(s) 1233, 1262, 2204
AcsI RAATTY 5 cut(s) 739, 753, 833, 917, 2321
AcuI CTGAAG 1 cut(s) 1114
AdeI CACNNNGTG 1 cut(s) 1913
AgeI ACCGGT 1 cut(s) 228
AjnI CCWGG 4 cut(s) 132, 152, 743, 1528
Alw21I GWGCWC 3 cut(s) 820, 1897, 2257
Alw26I GTCTC 3 cut(s) 632, 1959, 2010
Alw44I GTGCAC 1 cut(s) 816
AlwI GGATC 2 cut(s) 260, 698
AlwNI CAGNNNCTG 2 cut(s) 164, 405
Aor13HI TCCGGA 1 cut(s) 1299
AoxI GGCC 5 cut(s) 666, 1233, 1262, 1781, 2204
ApaLI GTGCAC 1 cut(s) 816
ApeKI GCWGC 7 cut(s) 575, 592, 857, 902, 1145, 1793, 1941
ApoI RAATTY 5 cut(s) 739, 753, 833, 917, 2321
ArsI GACNNNNNNTTYG 4 cut(s) 1705, 1737, 1869, 1901
AsiGI ACCGGT 1 cut(s) 228
Asp718I GGTACC 1 cut(s) 1158
AspLEI GCGC 4 cut(s) 1853, 2110, 2112, 2282
AspS9I GGNCC 5 cut(s) 184, 226, 1655, 1781, 2214
AsuC2I CCSGG 3 cut(s) 1271, 2176, 2203
AsuHPI GGTGA 4 cut(s) 18, 1073, 1269, 1611
AvaII GGWCC 4 cut(s) 184, 226, 1655, 2214
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 820
BalI TGGCCA 2 cut(s) 1235, 1264
BanI GGYRCC 2 cut(s) 1158, 1632
BanII GRGCYC 1 cut(s) 349
BauI CACGAG 1 cut(s) 2341
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 3 cut(s) 820, 1897, 2257
BbvI GCAGC 7 cut(s) 562, 579, 869, 889, 1132, 1780, 1953
BccI CCATC 5 cut(s) 22, 254, 1170, 1177, 1772
BciT130I CCWGG 4 cut(s) 134, 154, 745, 1530
BciVI GTATCC 1 cut(s) 1307
BclI TGATCA 1 cut(s) 1915
BcnI CCSGG 3 cut(s) 1271, 2176, 2203
BcoDI GTCTC 3 cut(s) 632, 1959, 2010
BfaI CTAG 2 cut(s) 1085, 1841
BfoI RGCGCY 1 cut(s) 1854
BfuI GTATCC 1 cut(s) 1307
BisI GCNGC 8 cut(s) 71, 576, 593, 858, 903, 1146, 1794, 1942
BlsI GCNGC 8 cut(s) 72, 577, 594, 859, 904, 1147, 1795, 1943
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 7 cut(s) 134, 154, 745, 1271, 1530, 2176, 2203
Bme18I GGWCC 4 cut(s) 184, 226, 1655, 2214
BmgT120I GGNCC 5 cut(s) 184, 226, 1655, 1781, 2214
BmiI GGNNCC 4 cut(s) 735, 971, 1160, 1634
BmrFI CCNGG 7 cut(s) 134, 154, 745, 1271, 1530, 2176, 2203
BmsI GCATC 8 cut(s) 152, 432, 1214, 1878, 2058, 2095, 2229, 2291
BpiI GAAGAC 1 cut(s) 573
BpmI CTGGAG 3 cut(s) 69, 1479, 1723
Bpu10I CCTNAGC 1 cut(s) 2290
BpuEI CTTGAG 2 cut(s) 1930, 2026
BpuMI CCSGG 3 cut(s) 1271, 2176, 2203
Bsa29I ATCGAT 1 cut(s) 498
BsaBI GATNNNNATC 2 cut(s) 708, 1116
BsaJI CCNNGG 2 cut(s) 152, 1270
BsaWI WCCGGW 3 cut(s) 228, 1299, 2182
BsaXI ACNNNNNCTCC 6 cut(s) 1035, 1065, 1452, 1482, 2297, 2327
Bse118I RCCGGY 2 cut(s) 228, 237
Bse1I ACTGG 7 cut(s) 86, 269, 410, 1442, 1462, 1706, 2216
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 4 cut(s) 253, 1361, 1431, 2138
Bse8I GATNNNNATC 2 cut(s) 708, 1116
BseAI TCCGGA 1 cut(s) 1299
BseBI CCWGG 4 cut(s) 134, 154, 745, 1530
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 152, 1270
BseGI GGATG 9 cut(s) 143, 210, 223, 312, 447, 1431, 1525, 1599, 1869
BseJI GATNNNNATC 2 cut(s) 708, 1116
BseMI GCAATG 4 cut(s) 253, 1361, 1431, 2138
BseMII CTCAG 6 cut(s) 164, 483, 546, 1571, 1625, 2127
BseNI ACTGG 7 cut(s) 86, 269, 410, 1442, 1462, 1706, 2216
BsePI GCGCGC 1 cut(s) 2108
BseRI GAGGAG 1 cut(s) 929
BseSI GKGCMC 1 cut(s) 820
BseXI GCAGC 7 cut(s) 562, 579, 869, 889, 1132, 1780, 1953
BsgI GTGCAG 1 cut(s) 380
Bsh1236I CGCG 1 cut(s) 2110
BshFI GGCC 5 cut(s) 668, 1235, 1264, 1783, 2206
BshNI GGYRCC 2 cut(s) 1158, 1632
BshTI ACCGGT 1 cut(s) 228
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 3 cut(s) 820, 1897, 2257
BsiSI CCGG 7 cut(s) 229, 238, 1270, 1300, 2176, 2183, 2203
BslFI GGGAC 2 cut(s) 1528, 1712
BsmAI GTCTC 3 cut(s) 632, 1959, 2010
BsmFI GGGAC 2 cut(s) 1528, 1712
BsmI GAATGC 2 cut(s) 1241, 1943
BsnI GGCC 5 cut(s) 668, 1235, 1264, 1783, 2206
Bsp1286I GDGCHC 4 cut(s) 349, 820, 1897, 2257
Bsp13I TCCGGA 1 cut(s) 1299
Bsp1407I TGTACA 2 cut(s) 1428, 1590
Bsp143I GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
BspACI CCGC 1 cut(s) 71
BspANI GGCC 5 cut(s) 668, 1235, 1264, 1783, 2206
BspCNI CTCAG 6 cut(s) 165, 484, 547, 1572, 1624, 2126
BspDI ATCGAT 1 cut(s) 498
BspEI TCCGGA 1 cut(s) 1299
BspFNI CGCG 1 cut(s) 2110
BspHI TCATGA 1 cut(s) 2022
BspLI GGNNCC 4 cut(s) 735, 971, 1160, 1634
BspPI GGATC 2 cut(s) 260, 698
BspQI GCTCTTC 1 cut(s) 2245
BspT107I GGYRCC 2 cut(s) 1158, 1632
BsrDI GCAATG 4 cut(s) 253, 1361, 1431, 2138
BsrFI RCCGGY 2 cut(s) 228, 237
BsrGI TGTACA 2 cut(s) 1428, 1590
BsrI ACTGG 7 cut(s) 86, 269, 410, 1442, 1462, 1706, 2216
BssAI RCCGGY 2 cut(s) 228, 237
BssECI CCNNGG 2 cut(s) 152, 1270
BssHII GCGCGC 1 cut(s) 2108
BssMI GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
BssSI CACGAG 1 cut(s) 2341
Bst2BI CACGAG 1 cut(s) 2341
Bst2UI CCWGG 4 cut(s) 134, 154, 745, 1530
Bst4CI ACNGT 8 cut(s) 30, 315, 401, 822, 1222, 1721, 1757, 2276
Bst6I CTCTTC 2 cut(s) 1394, 2245
BstAPI GCANNNNNTGC 2 cut(s) 366, 1938
BstAUI TGTACA 2 cut(s) 1428, 1590
BstC8I GCNNGC 3 cut(s) 123, 243, 2110
BstEII GGTNACC 1 cut(s) 1275
BstF5I GGATG 9 cut(s) 143, 210, 223, 312, 447, 1431, 1525, 1599, 1869
BstFNI CGCG 1 cut(s) 2110
BstH2I RGCGCY 1 cut(s) 1854
BstHHI GCGC 4 cut(s) 1853, 2110, 2112, 2282
BstKTI GATC 8 cut(s) 268, 498, 706, 767, 999, 1120, 1487, 1918
BstMAI GTCTC 3 cut(s) 632, 1959, 2010
BstMBI GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
BstMWI GCNNNNNNNGC 5 cut(s) 366, 847, 899, 1938, 2135
BstNI CCWGG 4 cut(s) 134, 154, 745, 1530
BstNSI RCATGY 2 cut(s) 1591, 2241
BstPI GGTNACC 1 cut(s) 1275
BstSCI CCNGG 7 cut(s) 132, 152, 743, 1269, 1528, 2174, 2201
BstSLI GKGCMC 1 cut(s) 820
BstUI CGCG 1 cut(s) 2110
BstV1I GCAGC 7 cut(s) 562, 579, 869, 889, 1132, 1780, 1953
BstV2I GAAGAC 1 cut(s) 573
BstXI CCANNNNNNTGG 1 cut(s) 1629
Bsu15I ATCGAT 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 555
BsuI GTATCC 1 cut(s) 1307
BsuRI GGCC 5 cut(s) 668, 1235, 1264, 1783, 2206
BsuTUI ATCGAT 1 cut(s) 498
BtgZI GCGATG 1 cut(s) 1021
BtsCI GGATG 9 cut(s) 143, 210, 223, 312, 447, 1431, 1525, 1599, 1869
BtsI GCAGTG 3 cut(s) 116, 189, 1283
BtsIMutI CAGTG 5 cut(s) 116, 189, 600, 818, 1283
Cac8I GCNNGC 3 cut(s) 123, 243, 2110
CaiI CAGNNNCTG 2 cut(s) 164, 405
CciI TCATGA 1 cut(s) 2022
CfoI GCGC 4 cut(s) 1853, 2110, 2112, 2282
Cfr10I RCCGGY 2 cut(s) 228, 237
Cfr13I GGNCC 5 cut(s) 184, 226, 1655, 1781, 2214
ClaI ATCGAT 1 cut(s) 498
CspAI ACCGGT 1 cut(s) 228
CspCI CAANNNNNGTGG 4 cut(s) 1182, 1217, 2329, 2364
DpnI GATC 8 cut(s) 267, 497, 705, 766, 998, 1119, 1486, 1917
DpnII GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
DraI TTTAAA 1 cut(s) 649
DraIII CACNNNGTG 1 cut(s) 1913
EaeI YGGCCR 3 cut(s) 1233, 1262, 2204
Eam1104I CTCTTC 2 cut(s) 1394, 2245
EarI CTCTTC 2 cut(s) 1394, 2245
Eco147I AGGCCT 1 cut(s) 668
Eco24I GRGCYC 1 cut(s) 349
Eco32I GATATC 1 cut(s) 1822
Eco47I GGWCC 4 cut(s) 184, 226, 1655, 2214
Eco57I CTGAAG 1 cut(s) 1114
Eco81I CCTNAGG 1 cut(s) 555
Eco91I GGTNACC 1 cut(s) 1275
EcoO65I GGTNACC 1 cut(s) 1275
EcoRII CCWGG 4 cut(s) 132, 152, 743, 1528
EcoRV GATATC 1 cut(s) 1822
EcoT22I ATGCAT 1 cut(s) 1363
EcoT38I GRGCYC 1 cut(s) 349
FalI AAGNNNNNCTT 4 cut(s) 656, 688, 1956, 1988
FaqI GGGAC 2 cut(s) 1528, 1712
FauNDI CATATG 1 cut(s) 809
FbaI TGATCA 1 cut(s) 1915
Fnu4HI GCNGC 8 cut(s) 71, 576, 593, 858, 903, 1146, 1794, 1942
FokI GGATG 9 cut(s) 130, 217, 230, 319, 454, 1438, 1512, 1586, 1856
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 8 cut(s) 71, 576, 593, 858, 903, 1146, 1794, 1942
FspBI CTAG 2 cut(s) 1085, 1841
GlaI GCGC 4 cut(s) 1852, 2109, 2111, 2281
GluI GCNGC 8 cut(s) 71, 576, 593, 858, 903, 1146, 1794, 1942
GsuI CTGGAG 3 cut(s) 69, 1479, 1723
HaeII RGCGCY 1 cut(s) 1854
HaeIII GGCC 5 cut(s) 668, 1235, 1264, 1783, 2206
HapII CCGG 7 cut(s) 229, 238, 1270, 1300, 2176, 2183, 2203
HhaI GCGC 4 cut(s) 1853, 2110, 2112, 2282
Hin6I GCGC 4 cut(s) 1851, 2108, 2110, 2280
HinP1I GCGC 4 cut(s) 1851, 2108, 2110, 2280
HincII GTYRAC 3 cut(s) 804, 991, 1647
HindII GTYRAC 3 cut(s) 804, 991, 1647
HinfI GANTC 5 cut(s) 98, 640, 979, 1112, 2179
HpaI GTTAAC 1 cut(s) 1647
HpaII CCGG 7 cut(s) 229, 238, 1270, 1300, 2176, 2183, 2203
HphI GGTGA 4 cut(s) 18, 1073, 1269, 1611
Hpy188I TCNGA 7 cut(s) 103, 160, 286, 698, 974, 1117, 1614
Hpy188III TCNNGA 8 cut(s) 731, 1300, 1488, 1653, 1746, 1947, 2023, 2096
Hpy99I CGWCG 1 cut(s) 780
HpyAV CCTTC 7 cut(s) 133, 473, 682, 969, 1290, 1507, 2020
HpyCH4III ACNGT 8 cut(s) 30, 315, 401, 822, 1222, 1721, 1757, 2276
HpyCH4IV ACGT 1 cut(s) 1404
HpyF10VI GCNNNNNNNGC 5 cut(s) 366, 847, 899, 1938, 2135
HpySE526I ACGT 1 cut(s) 1404
HspAI GCGC 4 cut(s) 1851, 2108, 2110, 2280
Kpn2I TCCGGA 1 cut(s) 1299
KpnI GGTACC 1 cut(s) 1162
Ksp22I TGATCA 1 cut(s) 1915
KspAI GTTAAC 1 cut(s) 1647
Kzo9I GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
LguI GCTCTTC 1 cut(s) 2245
LmnI GCTCC 4 cut(s) 589, 733, 975, 2003
Lsp1109I GCAGC 7 cut(s) 562, 579, 869, 889, 1132, 1780, 1953
LweI GCATC 8 cut(s) 152, 432, 1214, 1878, 2058, 2095, 2229, 2291
MaeI CTAG 2 cut(s) 1085, 1841
MaeII ACGT 1 cut(s) 1404
MaeIII GTNAC 8 cut(s) 401, 464, 1079, 1275, 1306, 1706, 1757, 1981
MalI GATC 8 cut(s) 267, 497, 705, 766, 998, 1119, 1486, 1917
MboI GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
MboII GAAGA 9 cut(s) 9, 27, 495, 578, 1120, 1381, 1673, 2215, 2262
MhlI GDGCHC 4 cut(s) 349, 820, 1897, 2257
MlsI TGGCCA 2 cut(s) 1235, 1264
MluNI TGGCCA 2 cut(s) 1235, 1264
MlyI GAGTC 2 cut(s) 634, 2173
MmeI TCCRAC 1 cut(s) 2283
Mox20I TGGCCA 2 cut(s) 1235, 1264
Mph1103I ATGCAT 1 cut(s) 1363
MroI TCCGGA 1 cut(s) 1299
MscI TGGCCA 2 cut(s) 1235, 1264
MseI TTAA 8 cut(s) 341, 504, 648, 915, 1646, 1752, 2081, 2371
MslI CAYNNNNRTG 3 cut(s) 305, 1627, 1776
Msp20I TGGCCA 2 cut(s) 1235, 1264
MspI CCGG 7 cut(s) 229, 238, 1270, 1300, 2176, 2183, 2203
MspR9I CCNGG 7 cut(s) 134, 154, 745, 1271, 1530, 2176, 2203
Mva1269I GAATGC 2 cut(s) 1241, 1943
MvaI CCWGG 4 cut(s) 134, 154, 745, 1530
MvnI CGCG 1 cut(s) 2110
MwoI GCNNNNNNNGC 5 cut(s) 366, 847, 899, 1938, 2135
NciI CCSGG 3 cut(s) 1271, 2176, 2203
NdeI CATATG 1 cut(s) 809
NdeII GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
NlaIV GGNNCC 4 cut(s) 735, 971, 1160, 1634
NmuCI GTSAC 5 cut(s) 464, 1079, 1275, 1306, 1981
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 2 cut(s) 1591, 2241
PagI TCATGA 1 cut(s) 2022
PauI GCGCGC 1 cut(s) 2108
PceI AGGCCT 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 2245
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 1471
PctI GAATGC 2 cut(s) 1241, 1943
PfeI GAWTC 3 cut(s) 98, 979, 1112
PflFI GACNNNGTC 1 cut(s) 1808
PfoI TCCNGGA 3 cut(s) 743, 1528, 2174
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 8 cut(s) 72, 577, 594, 859, 904, 1147, 1795, 1943
PleI GAGTC 2 cut(s) 634, 2173
PpsI GAGTC 2 cut(s) 634, 2173
PsiI TTATAA 1 cut(s) 275
Psp6I CCWGG 4 cut(s) 132, 152, 743, 1528
PspEI GGTNACC 1 cut(s) 1275
PspGI CCWGG 4 cut(s) 132, 152, 743, 1528
PspN4I GGNNCC 4 cut(s) 735, 971, 1160, 1634
PspPI GGNCC 5 cut(s) 184, 226, 1655, 1781, 2214
PstNI CAGNNNCTG 2 cut(s) 164, 405
PsyI GACNNNGTC 1 cut(s) 1808
PteI GCGCGC 1 cut(s) 2108
RseI CAYNNNNRTG 3 cut(s) 305, 1627, 1776
SapI GCTCTTC 1 cut(s) 2245
SaqAI TTAA 8 cut(s) 341, 504, 648, 915, 1646, 1752, 2081, 2371
SatI GCNGC 8 cut(s) 71, 576, 593, 858, 903, 1146, 1794, 1942
Sau3AI GATC 8 cut(s) 265, 495, 703, 764, 996, 1117, 1484, 1915
Sau96I GGNCC 5 cut(s) 184, 226, 1655, 1781, 2214
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 2 cut(s) 634, 2173
ScrFI CCNGG 7 cut(s) 134, 154, 745, 1271, 1530, 2176, 2203
SduI GDGCHC 4 cut(s) 349, 820, 1897, 2257
SfaNI GCATC 8 cut(s) 152, 432, 1214, 1878, 2058, 2095, 2229, 2291
SinI GGWCC 4 cut(s) 184, 226, 1655, 2214
SmiMI CAYNNNNRTG 3 cut(s) 305, 1627, 1776
SmlI CTYRAG 2 cut(s) 1945, 2041
SmoI CTYRAG 2 cut(s) 1945, 2041
SseBI AGGCCT 1 cut(s) 668
SsiI CCGC 1 cut(s) 71
SspI AATATT 1 cut(s) 1185
SspMI CTAG 2 cut(s) 1085, 1841
StuI AGGCCT 1 cut(s) 668
StyD4I CCNGG 7 cut(s) 132, 152, 743, 1269, 1528, 2174, 2201
TaaI ACNGT 8 cut(s) 30, 315, 401, 822, 1222, 1721, 1757, 2276
TaiI ACGT 1 cut(s) 1407
TaqI TCGA 5 cut(s) 498, 763, 995, 1474, 2327
TaqII GACCGA 2 cut(s) 738, 1826
TatI WGTACW 4 cut(s) 50, 1428, 1590, 1768
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 3 cut(s) 98, 979, 1112
Tru1I TTAA 8 cut(s) 341, 504, 648, 915, 1646, 1752, 2081, 2371
Tru9I TTAA 8 cut(s) 341, 504, 648, 915, 1646, 1752, 2081, 2371
TscAI CASTG 5 cut(s) 123, 196, 607, 825, 1290
TseFI GTSAC 5 cut(s) 464, 1079, 1275, 1306, 1981
TseI GCWGC 7 cut(s) 575, 592, 857, 902, 1145, 1793, 1941
Tsp45I GTSAC 5 cut(s) 464, 1079, 1275, 1306, 1981
TspDTI ATGAA 8 cut(s) 27, 323, 1336, 1425, 1514, 1588, 1852, 2039
TspGWI ACGGA 2 cut(s) 696, 2272
TspRI CASTG 5 cut(s) 123, 196, 607, 825, 1290
Tth111I GACNNNGTC 1 cut(s) 1808
VneI GTGCAC 1 cut(s) 816
VpaK11BI GGWCC 4 cut(s) 184, 226, 1655, 2214
XapI RAATTY 5 cut(s) 739, 753, 833, 917, 2321
XceI RCATGY 2 cut(s) 1591, 2241
XcmI CCANNNNNNNNNTGG 2 cut(s) 276, 1198
XspI CTAG 2 cut(s) 1085, 1841
ZrmI AGTACT 1 cut(s) 52
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.