pycom01g01920

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0024467
Physical Location & Seq
Forward (+)
1690717 .. 1693701
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g01920.6

Sequence Viewer

Length: 2217 bp
ATGACATCTAAAGTGGTACAAAAATATGGTATGAATAACATTACTCTATGCAAAAAGTGTGAAATCATGATGTATACTTTTTCTAACATTCGATATTTGCCTCTTAGGTTCATCTATAAAAACAATTTTATTTTTTGTCTCATTAATGAAGGAGTGGATCCTACCTTTTGGGAGGTCACTGGCTTCAGGTTCCGTAGGAACTCCGAAGTTAAGCGAGAGAGGGGCCAGAGCATTCCCAGGATGGGTGACCCACTGGGAAGTTCTTATGTGAGTTCCCAGAAACAAAACCTTGAGGGTGTGGTCGGGGCCCAAATCGGACAATATCGTGCTACGATGGATTTCCAAAAAACTGAAAAAATTAACAGAAGGACACATGTTAAGTACAAGATATTTCCATCCAGCAAAACTAAACTCTTGGTAGTTAAAATTAGGGGTGGAAAAAATTCCCAAAAATCTCGAACCAAATCGAAAAAAATACCAATCCCAAACCAAAAATGTCCCAAACTGATTTTCCCAAAATTTTCGATATTTTGGTATTCCCATACTGAACTGAAAATATATATTTTTAATTATTTTATATATATATATATATATATTTACACAGATAATAATATATATTATTCTTTTAATTATAGTCGTCCATTCCTTGTTAGGCTTTCTCCCCGTCAAGGACTCGAGTGTCGTTTTGAACTTTCGATTTCATTTCTCACTCACTCAATGTCTCAGCTCAACACCTCAGCCATTGAGAAAGCCCTCTCCTCTTCCTTTTTGTCTCAAATCCAAATCTGGCCAGCTAGTCACCAGCCCACCTCCACATGGATCATCCACTGCTGTATCTCTTCCACAGTGGACCCTTCCTTCATGACACAGAGCCATAGCCACCATCGATCTCTCTCTTGCGATTCTTTCTATTGCGACTCTCTCTGGCGATTCTCTCTCTTTTTCACGCGATTCTCTCTCTCAGATTCAGACATAGTTACGAATTCTAGATTAGGGTTTGTAAGGTTTGTAAATTTAAGGTTTGTTTGGTTTCACAGATTCTATTTAATTGATCCTATGGTTCTTGGGTTTTTGCAAATTGGTGAAGGTCTGAAGGAACTGGGCTCTTTTGCTTTATTTCTGCAGCTTCTATTAAAAACTTATGGATTAGAACTTATGTTCGATGTTTGTTCTTTTAACTTAGTCGTAATATATTTTGTTCATTGCCAACGGGCTAGACTGGAAAAGAGTGACCTGAACTTTCAGAATGTTGTACAAGATGTTCAGAGTTGTCAAATATTCTATGGTTTGTACTTATTGGTCCTTGTGAACCTTATGTATTTAAGCCTCTTGAGTTATAAGTGGTTGCTTGTCTACGAACATTTAAAATTTGAAGTCTATGGGTCAATTATTTTGGGGATGGAAGAAGACTACACTAATACAATGAAGTTGTCATTCCTCTATTCCTGTGTCATATTGCATTTCAGAACAATTATTTTCTTTGATTGGGTCCTTCTGTATGTTATGCCTATTATAGCCCAAAAGCCCAAAAATATTTCGGGATTCCCAAAAATTTGGTTTGGGATCGGTCTTGAAATTGGGAATACCGAAAATTTCAATTTAGGAATCAGGACTAAGGTTTTGGTTCGCTCTAGAGAGTCTTGTGAAGAAACTATAGGGAACATGGAAAGAAAGAGACACAGTAGACTATTTAGTTTGGAGATGCACACAATGCAAATGAAAAGTTACAACCATATCATCCACCTCACCAGATTCCTGCTGAACAAATCGAATCCAGCACGCTATACAGACAGACTTACGCACCCCCATCCCTCCCCCGTGTGCACAACGCACCCCGCACGTAAGAAATTGATGGATTCAGATTACCTAGTACCTTCCCATATGGGCTGGTTCCATTTGGCCGGAGGATCCGAAACCTGTTCAGATGCCCCGGCCAGGAGCTATGCTAAGTTCTTTAGGGATGTACCGAAGAGGAAAGAGGTGATATCCATTGAAGCTTTCGATGGCGTTAAGGATGGAACCATCTACAACACGGAAGTGTTCCGGAAAACGATTCTCCATATGCAGAGTTTTGTCAATACTAGTGGTTTCAACCTCACCAGTAATTTCATGTTTTTCCTTGAACAGGGTGTTGTAATTGGCGGCTCCCAGGTAATCTGTGATTCTCCACACTGCATAATAATTACGTTATATTCATCTAGCCTGCCGTGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

739

Amino Acids

86.16

Weight (kDa)

9.45

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024467)

Species Orthologous Gene IDs
malus_domestica MD05G1215700.v1.1
pyrus_communis pycom01g01920 pycom04g02240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1338
AasI GACNNNNNNGTC 1 cut(s) 678
AccI GTMKAC 3 cut(s) 74, 1353, 1684
AccII CGCG 1 cut(s) 949
AccIII TCCGGA 1 cut(s) 2043
AciI CCGC 2 cut(s) 1836, 2142
AclWI GGATC 7 cut(s) 152, 165, 827, 1046, 1571, 1902, 1915
AcoI YGGCCR 3 cut(s) 788, 1899, 1932
AcsI RAATTY 7 cut(s) 442, 518, 982, 1012, 1367, 1551, 1591
AcuI CTGAAG 2 cut(s) 169, 1112
AfaI GTAC 6 cut(s) 18, 383, 1254, 1292, 1872, 1965
AfiI CCNNNNNNNGG 5 cut(s) 242, 668, 816, 1935, 2125
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 7 cut(s) 689, 1373, 1574, 1597, 1994, 2092, 2123
AhlI ACTAGT 1 cut(s) 2081
AjnI CCWGG 3 cut(s) 236, 1934, 2148
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AleI CACNNNNGTG 1 cut(s) 2036
AluBI AGCT 5 cut(s) 727, 794, 1126, 1941, 1997
AluI AGCT 5 cut(s) 727, 794, 1126, 1941, 1997
Alw21I GWGCWC 1 cut(s) 1826
Alw26I GTCTC 4 cut(s) 143, 726, 777, 1669
Alw44I GTGCAC 1 cut(s) 1822
AlwI GGATC 7 cut(s) 152, 165, 827, 1046, 1571, 1902, 1915
Ama87I CYCGRG 1 cut(s) 674
Aor13HI TCCGGA 1 cut(s) 2043
AoxI GGCC 5 cut(s) 223, 306, 788, 1899, 1932
ApaI GGGCCC 1 cut(s) 310
ApaLI GTGCAC 1 cut(s) 1822
ApeKI GCWGC 1 cut(s) 1123
ApoI RAATTY 7 cut(s) 442, 518, 982, 1012, 1367, 1551, 1591
ArsI GACNNNNNNTTYG 2 cut(s) 1603, 1635
AseI ATTAAT 1 cut(s) 144
Asp700I GAANNNNTTC 1 cut(s) 442
AspS9I GGNCC 6 cut(s) 223, 306, 307, 850, 1300, 1489
AsuC2I CCSGG 1 cut(s) 1931
AsuHPI GGTGA 6 cut(s) 257, 791, 1094, 1738, 1993, 2089
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 3 cut(s) 850, 1300, 1489
BaeGI GKGCMC 2 cut(s) 310, 1826
BaeI ACNNNNGTAYC 2 cut(s) 817, 850
BalI TGGCCA 1 cut(s) 790
BamHI GGATCC 2 cut(s) 157, 1907
BanII GRGCYC 2 cut(s) 310, 1106
BbsI GAAGAC 1 cut(s) 1413
Bbv12I GWGCWC 1 cut(s) 1826
BbvCI CCTCAGC 1 cut(s) 736
BbvI GCAGC 1 cut(s) 1135
BceAI ACGGC 1 cut(s) 2191
BciT130I CCWGG 3 cut(s) 238, 1936, 2150
BcnI CCSGG 1 cut(s) 1931
BcoDI GTCTC 4 cut(s) 143, 726, 777, 1669
BcuI ACTAGT 1 cut(s) 2081
BfaI CTAG 7 cut(s) 795, 987, 1215, 1632, 1868, 2082, 2199
BfmI CTRYAG 2 cut(s) 1121, 1653
BisI GCNGC 2 cut(s) 1124, 2143
BlsI GCNGC 2 cut(s) 1125, 2144
Bme1390I CCNGG 4 cut(s) 238, 1931, 1936, 2150
Bme18I GGWCC 3 cut(s) 850, 1300, 1489
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 6 cut(s) 223, 306, 307, 850, 1300, 1489
BmrFI CCNGG 4 cut(s) 238, 1931, 1936, 2150
BmrI ACTGGG 2 cut(s) 263, 1109
BmsI GCATC 2 cut(s) 1692, 1915
BmuI ACTGGG 2 cut(s) 263, 1109
BpiI GAAGAC 1 cut(s) 1413
Bpu10I CCTNAGC 1 cut(s) 736
BpuEI CTTGAG 2 cut(s) 311, 1351
BpuMI CCSGG 1 cut(s) 1931
Bsa29I ATCGAT 1 cut(s) 886
BsaAI YACGTR 1 cut(s) 1841
BsaJI CCNNGG 4 cut(s) 236, 1929, 2148, 2207
BsaWI WCCGGW 1 cut(s) 2043
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 668, 816, 1935, 2125
Bse1I ACTGG 5 cut(s) 184, 258, 1104, 1224, 2100
Bse3DI GCAATG 1 cut(s) 1201
BseAI TCCGGA 1 cut(s) 2043
BseBI CCWGG 3 cut(s) 238, 1936, 2150
BseCI ATCGAT 1 cut(s) 886
BseDI CCNNGG 4 cut(s) 236, 1929, 2148, 2207
BseGI GGATG 8 cut(s) 246, 395, 822, 1404, 1737, 1807, 1966, 2020
BseLI CCNNNNNNNGG 5 cut(s) 242, 668, 816, 1935, 2125
BseMI GCAATG 1 cut(s) 1201
BseMII CTCAG 3 cut(s) 737, 750, 975
BseNI ACTGG 5 cut(s) 184, 258, 1104, 1224, 2100
BseRI GAGGAG 1 cut(s) 748
BseSI GKGCMC 2 cut(s) 310, 1826
BseXI GCAGC 1 cut(s) 1135
Bsh1236I CGCG 1 cut(s) 949
BshFI GGCC 5 cut(s) 225, 308, 790, 1901, 1934
BshVI ATCGAT 1 cut(s) 886
BsiHKAI GWGCWC 1 cut(s) 1826
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 3 cut(s) 1902, 1931, 2044
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 5 cut(s) 242, 668, 816, 1935, 2125
BsmAI GTCTC 4 cut(s) 143, 726, 777, 1669
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 5 cut(s) 225, 308, 790, 1901, 1934
BsoBI CYCGRG 1 cut(s) 674
Bsp120I GGGCCC 1 cut(s) 306
Bsp1286I GDGCHC 3 cut(s) 310, 1106, 1826
Bsp13I TCCGGA 1 cut(s) 2043
Bsp1407I TGTACA 1 cut(s) 1252
Bsp143I GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
BspACI CCGC 2 cut(s) 1836, 2142
BspANI GGCC 5 cut(s) 225, 308, 790, 1901, 1934
BspCNI CTCAG 3 cut(s) 736, 749, 974
BspDI ATCGAT 1 cut(s) 886
BspEI TCCGGA 1 cut(s) 2043
BspFNI CGCG 1 cut(s) 949
BspHI TCATGA 2 cut(s) 66, 861
BspMAI CTGCAG 1 cut(s) 1125
BspPI GGATC 7 cut(s) 152, 165, 827, 1046, 1571, 1902, 1915
BsrDI GCAATG 1 cut(s) 1201
BsrGI TGTACA 1 cut(s) 1252
BsrI ACTGG 5 cut(s) 184, 258, 1104, 1224, 2100
BssECI CCNNGG 4 cut(s) 236, 1929, 2148, 2207
BssMI GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
BssNAI GTATAC 1 cut(s) 75
Bst1107I GTATAC 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 238, 1936, 2150
Bst4CI ACNGT 2 cut(s) 847, 1682
Bst6I CTCTTC 3 cut(s) 766, 844, 1964
BstAPI GCANNNNNTGC 1 cut(s) 1711
BstAUI TGTACA 1 cut(s) 1252
BstBAI YACGTR 1 cut(s) 1841
BstC8I GCNNGC 3 cut(s) 792, 1780, 2204
BstDEI CTNAG 7 cut(s) 104, 723, 736, 961, 1180, 1614, 1947
BstDSI CCRYGG 1 cut(s) 2207
BstEII GGTNACC 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 2123
BstF5I GGATG 8 cut(s) 246, 395, 822, 1404, 1737, 1807, 1966, 2020
BstFNI CGCG 1 cut(s) 949
BstKTI GATC 6 cut(s) 160, 822, 890, 1054, 1566, 1910
BstMAI GTCTC 4 cut(s) 143, 726, 777, 1669
BstMBI GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
BstMWI GCNNNNNNNGC 1 cut(s) 1711
BstNI CCWGG 3 cut(s) 238, 1936, 2150
BstNSI RCATGY 1 cut(s) 377
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 4 cut(s) 236, 1929, 1934, 2148
BstSFI CTRYAG 2 cut(s) 1121, 1653
BstSLI GKGCMC 2 cut(s) 310, 1826
BstUI CGCG 1 cut(s) 949
BstV1I GCAGC 1 cut(s) 1135
BstV2I GAAGAC 1 cut(s) 1413
BstX2I RGATCY 2 cut(s) 157, 1907
BstXI CCANNNNNNTGG 1 cut(s) 1554
BstYI RGATCY 2 cut(s) 157, 1907
BstZ17I GTATAC 1 cut(s) 75
Bsu15I ATCGAT 1 cut(s) 886
BsuRI GGCC 5 cut(s) 225, 308, 790, 1901, 1934
BsuTUI ATCGAT 1 cut(s) 886
BtgI CCRYGG 1 cut(s) 2207
BtsCI GGATG 8 cut(s) 246, 395, 822, 1404, 1737, 1807, 1966, 2020
BtsI GCAGTG 2 cut(s) 826, 2170
BtsIMutI CAGTG 5 cut(s) 177, 251, 826, 852, 2170
Cac8I GCNNGC 3 cut(s) 792, 1780, 2204
CciI TCATGA 2 cut(s) 66, 861
Cfr13I GGNCC 6 cut(s) 223, 306, 307, 850, 1300, 1489
ClaI ATCGAT 1 cut(s) 886
Csp6I GTAC 6 cut(s) 17, 382, 1253, 1291, 1871, 1964
CspCI CAANNNNNGTGG 2 cut(s) 2065, 2100
CviAII CATG 6 cut(s) 67, 374, 816, 862, 1663, 2110
CviQI GTAC 6 cut(s) 17, 382, 1253, 1291, 1871, 1964
DdeI CTNAG 7 cut(s) 104, 723, 736, 961, 1180, 1614, 1947
DpnI GATC 6 cut(s) 159, 821, 889, 1053, 1565, 1909
DpnII GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
DraI TTTAAA 1 cut(s) 1365
DrdI GACNNNNNNGTC 1 cut(s) 678
DseDI GACNNNNNNGTC 1 cut(s) 678
EaeI YGGCCR 3 cut(s) 788, 1899, 1932
Eam1104I CTCTTC 3 cut(s) 766, 844, 1964
EarI CTCTTC 3 cut(s) 766, 844, 1964
Eco24I GRGCYC 2 cut(s) 310, 1106
Eco32I GATATC 1 cut(s) 1986
Eco47I GGWCC 3 cut(s) 850, 1300, 1489
Eco57I CTGAAG 2 cut(s) 169, 1112
Eco88I CYCGRG 1 cut(s) 674
Eco91I GGTNACC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 2123
EcoO109I RGGNCCY 2 cut(s) 306, 1489
EcoO65I GGTNACC 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 3 cut(s) 236, 1934, 2148
EcoRV GATATC 1 cut(s) 1986
EcoT38I GRGCYC 2 cut(s) 310, 1106
FaeI CATG 6 cut(s) 70, 377, 819, 865, 1666, 2113
FaqI GGGAC 1 cut(s) 483
FatI CATG 6 cut(s) 66, 373, 815, 861, 1662, 2109
FauI CCCGC 1 cut(s) 1843
FauNDI CATATG 2 cut(s) 1881, 2061
FblI GTMKAC 3 cut(s) 74, 1353, 1684
Fnu4HI GCNGC 2 cut(s) 1124, 2143
FokI GGATG 8 cut(s) 253, 382, 809, 1411, 1724, 1794, 1973, 2027
FriOI GRGCYC 2 cut(s) 310, 1106
Fsp4HI GCNGC 2 cut(s) 1124, 2143
FspBI CTAG 7 cut(s) 795, 987, 1215, 1632, 1868, 2082, 2199
GluI GCNGC 2 cut(s) 1124, 2143
HaeIII GGCC 5 cut(s) 225, 308, 790, 1901, 1934
HapII CCGG 3 cut(s) 1902, 1931, 2044
Hin1II CATG 6 cut(s) 70, 377, 819, 865, 1666, 2113
HindIII AAGCTT 1 cut(s) 1995
HpaII CCGG 3 cut(s) 1902, 1931, 2044
HphI GGTGA 6 cut(s) 257, 791, 1094, 1738, 1993, 2089
Hpy166II GTNNAC 6 cut(s) 75, 850, 1309, 1354, 1685, 1824
Hpy8I GTNNAC 6 cut(s) 75, 850, 1309, 1354, 1685, 1824
HpyAV CCTTC 8 cut(s) 143, 360, 864, 868, 1079, 1087, 1502, 1884
HpyCH4III ACNGT 2 cut(s) 847, 1682
HpyCH4IV ACGT 2 cut(s) 1840, 2186
HpyCH4V TGCA 9 cut(s) 51, 1075, 1123, 1459, 1705, 1714, 1824, 2065, 2175
HpyF10VI GCNNNNNNNGC 1 cut(s) 1711
HpyF3I CTNAG 7 cut(s) 104, 723, 736, 961, 1180, 1614, 1947
HpySE526I ACGT 2 cut(s) 1840, 2186
Hsp92II CATG 6 cut(s) 70, 377, 819, 865, 1666, 2113
Kpn2I TCCGGA 1 cut(s) 2043
Kzo9I GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
LmnI GCTCC 2 cut(s) 1938, 2150
Lsp1109I GCAGC 1 cut(s) 1135
LweI GCATC 2 cut(s) 1692, 1915
MaeI CTAG 7 cut(s) 795, 987, 1215, 1632, 1868, 2082, 2199
MaeII ACGT 2 cut(s) 1840, 2186
MaeIII GTNAC 6 cut(s) 175, 245, 797, 976, 1229, 1724
MalI GATC 6 cut(s) 159, 821, 889, 1053, 1565, 1909
MboI GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
MboII GAAGA 6 cut(s) 753, 831, 1415, 1418, 1658, 1981
MflI RGATCY 2 cut(s) 157, 1907
MhlI GDGCHC 3 cut(s) 310, 1106, 1826
MlsI TGGCCA 1 cut(s) 790
MluNI TGGCCA 1 cut(s) 790
MlyI GAGTC 3 cut(s) 666, 911, 1646
Mox20I TGGCCA 1 cut(s) 790
MroI TCCGGA 1 cut(s) 2043
MroXI GAANNNNTTC 1 cut(s) 442
MscI TGGCCA 1 cut(s) 790
MslI CAYNNNNRTG 1 cut(s) 2036
Msp20I TGGCCA 1 cut(s) 790
MspI CCGG 3 cut(s) 1902, 1931, 2044
MspR9I CCNGG 4 cut(s) 238, 1931, 1936, 2150
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 3 cut(s) 238, 1936, 2150
MvnI CGCG 1 cut(s) 949
MwoI GCNNNNNNNGC 1 cut(s) 1711
NciI CCSGG 1 cut(s) 1931
NdeI CATATG 2 cut(s) 1881, 2061
NdeII GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
NlaIII CATG 6 cut(s) 70, 377, 819, 865, 1666, 2113
NmuCI GTSAC 4 cut(s) 175, 245, 797, 1229
NspI RCATGY 1 cut(s) 377
OliI CACNNNNGTG 1 cut(s) 2036
PaeR7I CTCGAG 1 cut(s) 674
PagI TCATGA 2 cut(s) 66, 861
PciI ACATGT 1 cut(s) 373
PctI GAATGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 442
PkrI GCNGC 2 cut(s) 1125, 2144
PleI GAGTC 3 cut(s) 666, 911, 1645
PpsI GAGTC 3 cut(s) 666, 911, 1645
Ppu21I YACGTR 1 cut(s) 1841
PpuMI RGGWCCY 1 cut(s) 1489
PscI ACATGT 1 cut(s) 373
PshBI ATTAAT 1 cut(s) 144
PsiI TTATAA 1 cut(s) 1338
Psp5II RGGWCCY 1 cut(s) 1489
Psp6I CCWGG 3 cut(s) 236, 1934, 2148
PspEI GGTNACC 1 cut(s) 245
PspGI CCWGG 3 cut(s) 236, 1934, 2148
PspOMI GGGCCC 1 cut(s) 306
PspPI GGNCC 6 cut(s) 223, 306, 307, 850, 1300, 1489
PspPPI RGGWCCY 1 cut(s) 1489
PspXI VCTCGAGB 1 cut(s) 674
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PstI CTGCAG 1 cut(s) 1125
PsuI RGATCY 2 cut(s) 157, 1907
RsaI GTAC 6 cut(s) 18, 383, 1254, 1292, 1872, 1965
RsaNI GTAC 6 cut(s) 17, 382, 1253, 1291, 1871, 1964
RseI CAYNNNNRTG 1 cut(s) 2036
SatI GCNGC 2 cut(s) 1124, 2143
Sau3AI GATC 6 cut(s) 157, 819, 887, 1051, 1563, 1907
Sau96I GGNCC 6 cut(s) 223, 306, 307, 850, 1300, 1489
SchI GAGTC 3 cut(s) 666, 911, 1646
ScrFI CCNGG 4 cut(s) 238, 1931, 1936, 2150
SduI GDGCHC 3 cut(s) 310, 1106, 1826
SfaNI GCATC 2 cut(s) 1692, 1915
SfcI CTRYAG 2 cut(s) 1121, 1653
Sfr274I CTCGAG 1 cut(s) 674
SinI GGWCC 3 cut(s) 850, 1300, 1489
SlaI CTCGAG 1 cut(s) 674
SmiMI CAYNNNNRTG 1 cut(s) 2036
SmlI CTYRAG 3 cut(s) 290, 674, 1330
SmoI CTYRAG 3 cut(s) 290, 674, 1330
SpeI ACTAGT 1 cut(s) 2081
SsiI CCGC 2 cut(s) 1836, 2142
SspI AATATT 2 cut(s) 1278, 1534
SspMI CTAG 7 cut(s) 795, 987, 1215, 1632, 1868, 2082, 2199
StyD4I CCNGG 4 cut(s) 236, 1929, 1934, 2148
TaaI ACNGT 2 cut(s) 847, 1682
TaiI ACGT 2 cut(s) 1843, 2189
TaqII GACCGA 1 cut(s) 1556
TatI WGTACW 3 cut(s) 381, 1252, 1290
TauI GCSGC 1 cut(s) 2145
TscAI CASTG 5 cut(s) 184, 258, 833, 852, 2177
TseFI GTSAC 4 cut(s) 175, 245, 797, 1229
TseI GCWGC 1 cut(s) 1123
Tsp45I GTSAC 4 cut(s) 175, 245, 797, 1229
TspGWI ACGGA 2 cut(s) 182, 2048
TspRI CASTG 5 cut(s) 184, 258, 833, 852, 2177
VneI GTGCAC 1 cut(s) 1822
VpaK11BI GGWCC 3 cut(s) 850, 1300, 1489
VspI ATTAAT 1 cut(s) 144
XagI CCTNNNNNAGG 1 cut(s) 2123
XapI RAATTY 7 cut(s) 442, 518, 982, 1012, 1367, 1551, 1591
XbaI TCTAGA 2 cut(s) 986, 1631
XceI RCATGY 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 674
XmiI GTMKAC 3 cut(s) 74, 1353, 1684
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 7 cut(s) 795, 987, 1215, 1632, 1868, 2082, 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.