pycom01g03590

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0022518
Physical Location & Seq
Forward (+)
2708231 .. 2709029
799 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03590.3

Sequence Viewer

Length: 675 bp
ATGATCCAATGGTCGAATCATAACCAAACAACAGAATTTCATCAATCGGATGTCTATTATGAAATCGGGGCACCCATTTTAGCCTTGGGATGTCAAGGGGTGTCTTTCCCCACGCACCACTGCATGCGGCAGCTGGTAGCCCCGACTCGCCAGAAAATTCAACTATTTCCAAAAATTCTCAATTTTCATAGGAATGAAGATCTCAACGAGAGCAACAACTTTGATACCTGTCGCCAAGTCCAATTTGGCCGGGAAAAGCTCCAATTCCCCTCAAAGGCTCTGAAAACCCTAGAAATGGGTGGCTTCAATCTACCACCTCCGGTGCTCCAGAAGTGTTTTGGTGGTGTTGGTGGTGGTCAACCTAACTTTCTCGATCGGTGGATTTCCGCCGTGGTGAAGGAAATATTGGTGAAAACACGTTCATTTGAGCAACATCTATGCATGCAATTAACAATTAAAAGGCGAAATCATGCTTGTATGCACTCAAAACAAAAATTTACCCATGAAATTCAAAGCCTAGTAGTTATGATGAACCAATACTCAACTCAAGATCTAAGAAAAAGACAAAGATTAATCACCCATGTGAGGGCCTTCCTTCCTTTGTCACCTTGCTCCTTCACTCCATGGATGTGGATATTGGGTTCTCCAAAAGTCCCCAAAGTTGGAGACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

225

Amino Acids

25.89

Weight (kDa)

9.65

Isoelectric Point (pI)

61.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022518)

Species Orthologous Gene IDs
pyrus_communis pycom01g00410 pycom01g03590 pycom05g22220 pycom05g22240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AciI CCGC 2 cut(s) 127, 387
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 5 cut(s) 35, 156, 174, 494, 507
AfiI CCNNNNNNNGG 5 cut(s) 274, 295, 585, 586, 662
AflIII ACRYGT 1 cut(s) 416
AgsI TTSAA 3 cut(s) 161, 307, 512
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AleI CACNNNNGTG 1 cut(s) 581
AloI GAACNNNNNNTCC 2 cut(s) 625, 657
AluBI AGCT 2 cut(s) 133, 259
AluI AGCT 2 cut(s) 133, 259
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 660
AoxI GGCC 2 cut(s) 247, 588
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 5 cut(s) 35, 156, 174, 494, 507
AseI ATTAAT 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 588
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 4 cut(s) 406, 421, 568, 597
BaeGI GKGCMC 1 cut(s) 73
BanI GGYRCC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 142
BceAI ACGGC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 2 cut(s) 290, 518
BglII AGATCT 2 cut(s) 199, 550
BisI GCNGC 2 cut(s) 128, 131
BlsI GCNGC 2 cut(s) 129, 132
Bme1390I CCNGG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 588
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 531
BpuMI CCSGG 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 3 cut(s) 84, 390, 623
BsaWI WCCGGW 1 cut(s) 319
Bsc4I CCNNNNNNNGG 5 cut(s) 274, 295, 585, 586, 662
BseDI CCNNGG 3 cut(s) 84, 390, 623
BseGI GGATG 3 cut(s) 55, 95, 633
BseLI CCNNNNNNNGG 5 cut(s) 274, 295, 585, 586, 662
BseSI GKGCMC 1 cut(s) 73
BseXI GCAGC 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 2 cut(s) 249, 590
BshNI GGYRCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 2 cut(s) 250, 320
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 5 cut(s) 274, 295, 585, 586, 662
BsmAI GTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 2 cut(s) 249, 590
Bso31I GGTCTC 1 cut(s) 660
Bsp1286I GDGCHC 2 cut(s) 73, 327
Bsp143I GATC 4 cut(s) 3, 199, 373, 550
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 2 cut(s) 127, 387
BspANI GGCC 2 cut(s) 249, 590
BspLI GGNNCC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 660
BssECI CCNNGG 3 cut(s) 84, 390, 623
BssMI GATC 4 cut(s) 3, 199, 373, 550
BssT1I CCWWGG 2 cut(s) 84, 623
BstC8I GCNNGC 2 cut(s) 125, 443
BstDEI CTNAG 1 cut(s) 554
BstDSI CCRYGG 2 cut(s) 390, 623
BstF5I GGATG 3 cut(s) 55, 95, 633
BstKTI GATC 4 cut(s) 6, 202, 376, 553
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 4 cut(s) 3, 199, 373, 550
BstMCI CGRYCG 1 cut(s) 376
BstNSI RCATGY 2 cut(s) 127, 445
BstSCI CCNGG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 73
BstV1I GCAGC 1 cut(s) 142
BstX2I RGATCY 2 cut(s) 199, 550
BstXI CCANNNNNNTGG 1 cut(s) 630
BstYI RGATCY 2 cut(s) 199, 550
BsuRI GGCC 2 cut(s) 249, 590
BtgI CCRYGG 2 cut(s) 390, 623
BtsCI GGATG 3 cut(s) 55, 95, 633
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 2 cut(s) 125, 443
Cfr13I GGNCC 1 cut(s) 588
CviAII CATG 6 cut(s) 124, 442, 470, 503, 581, 624
CviJI RGCY 9 cut(s) 83, 133, 140, 249, 259, 278, 303, 516, 590
CviKI_1 RGCY 9 cut(s) 83, 133, 140, 249, 259, 278, 303, 516, 590
DdeI CTNAG 1 cut(s) 554
DpnI GATC 4 cut(s) 5, 201, 375, 552
DpnII GATC 4 cut(s) 3, 199, 373, 550
EaeI YGGCCR 1 cut(s) 247
EciI GGCGGA 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 84, 623
Eco31I GGTCTC 1 cut(s) 660
EcoO109I RGGNCCY 1 cut(s) 588
EcoT14I CCWWGG 2 cut(s) 84, 623
EcoT22I ATGCAT 1 cut(s) 443
ErhI CCWWGG 2 cut(s) 84, 623
FaeI CATG 6 cut(s) 127, 445, 473, 506, 584, 627
FaqI GGGAC 1 cut(s) 638
FatI CATG 6 cut(s) 123, 441, 469, 502, 580, 623
Fnu4HI GCNGC 2 cut(s) 128, 131
FokI GGATG 3 cut(s) 62, 102, 640
Fsp4HI GCNGC 2 cut(s) 128, 131
FspBI CTAG 2 cut(s) 290, 518
GluI GCNGC 2 cut(s) 128, 131
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 2 cut(s) 249, 590
HapII CCGG 2 cut(s) 250, 320
Hin1II CATG 6 cut(s) 127, 445, 473, 506, 584, 627
HincII GTYRAC 1 cut(s) 359
HindII GTYRAC 1 cut(s) 359
HinfI GANTC 2 cut(s) 16, 145
HpaII CCGG 2 cut(s) 250, 320
HphI GGTGA 4 cut(s) 406, 421, 568, 597
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 2 cut(s) 49, 282
Hpy188III TCNNGA 3 cut(s) 328, 371, 548
Hpy8I GTNNAC 1 cut(s) 359
HpyAV CCTTC 4 cut(s) 391, 601, 605, 625
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 4 cut(s) 123, 441, 445, 481
HpyF3I CTNAG 1 cut(s) 554
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 6 cut(s) 127, 445, 473, 506, 584, 627
Kzo9I GATC 4 cut(s) 3, 199, 373, 550
LmnI GCTCC 3 cut(s) 264, 330, 617
LpnPI CCDG 6 cut(s) 119, 164, 241, 263, 333, 341
Lsp1109I GCAGC 1 cut(s) 142
MaeI CTAG 2 cut(s) 290, 518
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 1 cut(s) 603
MalI GATC 4 cut(s) 5, 201, 375, 552
MboI GATC 4 cut(s) 3, 199, 373, 550
MboII GAAGA 1 cut(s) 209
MflI RGATCY 2 cut(s) 199, 550
MhlI GDGCHC 2 cut(s) 73, 327
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 643
MnlI CCTC 3 cut(s) 280, 327, 579
Mph1103I ATGCAT 1 cut(s) 443
MseI TTAA 3 cut(s) 449, 456, 572
MslI CAYNNNNRTG 3 cut(s) 192, 581, 628
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 2 cut(s) 250, 320
MspR9I CCNGG 1 cut(s) 251
NciI CCSGG 1 cut(s) 251
NcoI CCATGG 1 cut(s) 623
NdeII GATC 4 cut(s) 3, 199, 373, 550
NlaIII CATG 6 cut(s) 127, 445, 473, 506, 584, 627
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 603
NsiI ATGCAT 1 cut(s) 443
NspI RCATGY 2 cut(s) 127, 445
OliI CACNNNNGTG 1 cut(s) 581
PaeI GCATGC 2 cut(s) 127, 445
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 2 cut(s) 129, 132
Ple19I CGATCG 1 cut(s) 376
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PshBI ATTAAT 1 cut(s) 572
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 588
PsuI RGATCY 2 cut(s) 199, 550
PvuI CGATCG 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 133
RseI CAYNNNNRTG 3 cut(s) 192, 581, 628
SaqAI TTAA 3 cut(s) 449, 456, 572
SatI GCNGC 2 cut(s) 128, 131
Sau3AI GATC 4 cut(s) 3, 199, 373, 550
Sau96I GGNCC 1 cut(s) 588
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 251
SduI GDGCHC 2 cut(s) 73, 327
SetI ASST 8 cut(s) 135, 230, 261, 319, 364, 421, 610, 672
SmiMI CAYNNNNRTG 3 cut(s) 192, 581, 628
SmlI CTYRAG 1 cut(s) 546
SmoI CTYRAG 1 cut(s) 546
SphI GCATGC 2 cut(s) 127, 445
SsiI CCGC 2 cut(s) 127, 387
SspI AATATT 1 cut(s) 405
SspMI CTAG 2 cut(s) 290, 518
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 2 cut(s) 84, 623
TaiI ACGT 1 cut(s) 421
TaqI TCGA 2 cut(s) 14, 372
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 3 cut(s) 449, 456, 572
Tru9I TTAA 3 cut(s) 449, 456, 572
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 1 cut(s) 603
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 603
TspDTI ATGAA 7 cut(s) 29, 75, 176, 210, 411, 519, 545
TspRI CASTG 1 cut(s) 125
VspI ATTAAT 1 cut(s) 572
XapI RAATTY 5 cut(s) 35, 156, 174, 494, 507
XceI RCATGY 2 cut(s) 127, 445
XcmI CCANNNNNNNNNTGG 3 cut(s) 82, 242, 335
XspI CTAG 2 cut(s) 290, 518
Zsp2I ATGCAT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.