Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0021174

Physical Location & Seq

Genomic Coordinates Forward (+)

13029686 .. 13032626

Gene Structure 2941 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2370 bp

ATGTTTAATGTGAGTGCTTTCAGCATGTGGGGATTTGAAAGACTTATTGTGAGATCTGGGAAACAATCAAAATGTATTGCACTGAAATCAGTGAATCTTGCATCTCTTAGACACAACAAAATAGAGGTATTGATGCCTTTTCTTATAATTGTAACCTTCTTTGCGATGAGTTTTTATTTGATCCGGTGTAAACAATTGGCAAGTTTCATGTTCTTTACTAAGAGTGTAGGCTTTTTCAACTGTTCTGACATGATATTTTTTGATGAAACTTTGGGGATATTCCAAAGTTTGACTGTGCACATTTCTTTCTGTTTAAGGCCGGTCGTTGAATTTGACTACTTGTCACCTCAGCTTACGTCCCTTGTTAGATTATTAATACATGTATATATTTATATAGATTATAATCTCCATCTATACTGTAATAACACGTCTATGCAAAAACTTGACAGCAATGCGCTTTTTCATTTTTCGGATTACGATATAAATATGCCGATGATCTTCATCCGTCGAATGTTTGTGATCTCCGCATGCATGCTTAGCTTGGTCAGTTTGACCAGTGGCCGTGATCCTGATATACAGACTTACCGTAGCTGCGACGCCACGACTCCCACCTATGACTGGTACAGTTCCGAATTCCCGTCCAATCTAAACGACCTCTTCTCCGCCCTCAACTCCAATGCCACCAGAGAAACAGGCTACTACAACGCCACCTTTGGTAAGAAACTAACTGAAATGGCACACGGCTGCTTCCTCTGTCGTGGTGACGTTGAGGTAGACGTTTGCGCACAATGCGTGAACGCGGTAATCCTAATGGCAGTCCGGAACTGCCCCACAAGTAAACGAGTTGTGTGGTGGTACGGCAAGAACTGTATGTTAAGGTACTCAGATGAGTCATTCTTTTCCATTGTGCAATATTTGCCCGATATCACCGACATGATAGTTTCCATCCTGCGAGTACCGAAAGTTAATGCAACAATACCAGATGAACCAATCGGATTTAATCAGGTAATGGAGAGTATTTTGAATGATTTAGTCACCGAGGCTTCCAAGGCTTTTTCCAAGGAAGCGCATGTTACAGGGTTGAATATTACGTTGTACAGCCTTGCGCAGTGCACGCAGGACCTCTCCACTGCAGATTGCAATTCCTGTCTTAGGGTAGCCGTAGCACAAGTAGTTTCAGCCAATATAGGAATCAATGGAGGAAAATTTATGTGTCCCAGTTGTAATTTCAGGTACGAAACTGTCCCCTTTTACGGGAAACCACGGGTTCATCATCTTCCTCCTCCTCCTCCTCAAGGTAATAGAGTTGATGATATAAATATACACATTTATATTAGTGCATGTCAAGTTGCCGTCACACTAAACGGGTATGAGAAATCTTATCTTGCCTTGGTATCAAATCTAAACGGATCCTTGGAAAACGGTTTTCAAAAGCTTATATTAAAAAACTTGTTCATGTATAGGAAGAAGAAAACCATTGTCAGTAATCGTCGCCAGTATTGTTGCTTCTGTTACTGTCTGAGGAGATCATCACGAAACAAGCATAAAGTCCAGGGAGAAGAAAAGAATGGTAAAAAGAAGAAGCAAACAATTAAATTCAGTCGATCACTTGTTCTTTTATATTTTCCTATGTCAATTACGTGTAATCTAGCAGCTGTTGAGTCCTTGCAACTTGACTTGGAAACTATTGAAACTGCAACAAACAAGTTTTCTGATAGTAATAAGTTAGGTCAAGGCGGATTTGGTGAAGTTTTCAAGCATAATTCCTACCTGACAGGGATATATATTTTTCAGGGAACGCTTGGTGTTAATGGACAAGAAATAGCGGTAAAGAGGTTGTCAAAAAGCTCGGTGCAAGGTGTACAAGAGCTTAAGAATGAACTTCTATTGATAGCCAAGCTTCAGCACAGAAATCTAGTGAAGCTTCTGGGATTTTGCTTGGAAGGAGAAGAAACCATACTTGTCTATGAATATGTGCCTGACAAAAGTCCTGATTATTTTCTAAATGGTGTGGTTTGTTTGTTAACTGTCTGTTATTTCTTGCATTATAGAATTGAAAAACGAGAACAATTGGATTGGTCGAGACATTGCATGATAATAGGAGGAATAGCTCAAGAAATTCTATATCTTCATAAAGATTCAAGGCTTAGAGTTATACATCGCGATTTGAAACCGAGCAATATCCTATTAGATGTTAATATGAATCCAAAAATATCAAACTTTGGGATGGCTAGAATGTTTGGAGTTGATGATCAAACTGAAGGAAACACGAGAAGAATTGACGGTACTTATTATATACCTATATATATTGACTTTGATAACTCATCTTTGATTGCAAGTATTTTGGATGTGCAATTAGTGGTTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

790

Amino Acids

89.84

Weight (kDa)

8.55

Isoelectric Point (pI)

44.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	198 - 296	2.9e-18	Salt stress response/antifungal
Stress-antifung	PF01657	332 - 414	2.2e-09	Salt stress response/antifungal
Pkinase	PF00069	571 - 752	3.1e-22	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	573 - 754	1.8e-23	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0021174)

Species	Orthologous Gene IDs
malus_domestica	MD01G1096800.v1.1 MD01G1098100.v1.1
pyrus_communis	pycom01g02380 pycom01g12210 pycom13g25000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	146, 402
Acc16I	TGCGCA	2 cut(s)	784, 1107
AccI	GTMKAC	1 cut(s)	774
AccII	CGCG	2 cut(s)	800, 2163
AccIII	TCCGGA	1 cut(s)	819
AciI	CCGC	5 cut(s)	525, 663, 800, 1737, 1826
AclWI	GGATC	4 cut(s)	175, 560, 1404, 1417
AcoI	YGGCCR	1 cut(s)	559
AcsI	RAATTY	5 cut(s)	329, 632, 1205, 1595, 2118
AcuI	CTGAAG	2 cut(s)	1886, 2280
AcyI	GRCGYC	1 cut(s)	597
AfaI	GTAC	8 cut(s)	623, 857, 881, 957, 1097, 1235, 1863, 2287
AfiI	CCNNNNNNNGG	5 cut(s)	618, 1152, 1253, 1254, 1295
AflII	CTTAAG	1 cut(s)	1871
AflIII	ACRYGT	3 cut(s)	379, 426, 1640
AhdI	GACNNNNNGTC	1 cut(s)	340
AjiI	CACGTC	1 cut(s)	429
AjnI	CCWGG	1 cut(s)	1551
Alw21I	GWGCWC	2 cut(s)	300, 1115
Alw26I	GTCTC	1 cut(s)	2077
Alw44I	GTGCAC	2 cut(s)	296, 1111
AlwI	GGATC	4 cut(s)	175, 560, 1404, 1417
Aor13HI	TCCGGA	1 cut(s)	819
AoxI	GGCC	2 cut(s)	317, 559
ApaLI	GTGCAC	2 cut(s)	296, 1111
ApeKI	GCWGC	3 cut(s)	591, 744, 1652
ApoI	RAATTY	5 cut(s)	329, 632, 1205, 1595, 2118
AseI	ATTAAT	1 cut(s)	374
AspLEI	GCGC	4 cut(s)	457, 785, 1069, 1108
AspS9I	GGNCC	1 cut(s)	1120
AsuHPI	GGTGA	5 cut(s)	336, 773, 919, 1027, 1757
AvaII	GGWCC	1 cut(s)	1120
BaeGI	GKGCMC	2 cut(s)	300, 1115
BamHI	GGATCC	1 cut(s)	1409
BarI	GAAGNNNNNNTAC	4 cut(s)	1490, 1522, 1941, 1973
BauI	CACGAG	1 cut(s)	2269
Bbv12I	GWGCWC	2 cut(s)	300, 1115
BbvCI	CCTCAGC	1 cut(s)	348
BbvI	GCAGC	3 cut(s)	578, 731, 1664
BccI	CCATC	3 cut(s)	417, 953, 2221
BceAI	ACGGC	5 cut(s)	546, 757, 874, 1145, 1337
BciT130I	CCWGG	1 cut(s)	1553
BclI	TGATCA	1 cut(s)	2251
BcoDI	GTCTC	1 cut(s)	2077
BfaI	CTAG	3 cut(s)	1649, 1916, 2232
BfmI	CTRYAG	1 cut(s)	1131
BfrI	CTTAAG	1 cut(s)	1871
BglII	AGATCT	1 cut(s)	53
BisI	GCNGC	3 cut(s)	592, 745, 1653
BlpI	GCTNAGC	1 cut(s)	536
BlsI	GCNGC	3 cut(s)	593, 746, 1654
Bme1390I	CCNGG	1 cut(s)	1553
Bme18I	GGWCC	1 cut(s)	1120
BmeRI	GACNNNNNGTC	1 cut(s)	340
BmgBI	CACGTC	1 cut(s)	429
BmgT120I	GGNCC	1 cut(s)	1120
BmiI	GGNNCC	1 cut(s)	1411
BmrFI	CCNGG	1 cut(s)	1553
BmrI	ACTGGG	1 cut(s)	1212
BmsI	GCATC	2 cut(s)	110, 123
BmuI	ACTGGG	1 cut(s)	1212
BoxI	GACNNNNGTC	1 cut(s)	1986
Bpu10I	CCTNAGC	1 cut(s)	348
Bpu1102I	GCTNAGC	1 cut(s)	536
BpuEI	CTTGAG	2 cut(s)	1278, 2097
BsaAI	YACGTR	1 cut(s)	1641
BsaBI	GATNNNNATC	2 cut(s)	402, 500
BsaHI	GRCGYC	1 cut(s)	597
BsaJI	CCNNGG	7 cut(s)	1038, 1047, 1059, 1262, 1389, 1413, 1552
BsaWI	WCCGGW	2 cut(s)	183, 819
BsaXI	ACNNNNNCTCC	2 cut(s)	644, 674
Bsc4I	CCNNNNNNNGG	5 cut(s)	618, 1152, 1253, 1254, 1295
Bse118I	RCCGGY	1 cut(s)	319
Bse1I	ACTGG	4 cut(s)	555, 623, 1218, 1495
Bse3DI	GCAATG	2 cut(s)	457, 2086
Bse8I	GATNNNNATC	2 cut(s)	402, 500
BseAI	TCCGGA	1 cut(s)	819
BseBI	CCWGG	1 cut(s)	1553
BseDI	CCNNGG	7 cut(s)	1038, 1047, 1059, 1262, 1389, 1413, 1552
BseGI	GGATG	4 cut(s)	501, 945, 2232, 2353
BseJI	GATNNNNATC	2 cut(s)	402, 500
BseLI	CCNNNNNNNGG	5 cut(s)	618, 1152, 1253, 1254, 1295
BseMI	GCAATG	2 cut(s)	457, 2086
BseMII	CTCAG	3 cut(s)	362, 897, 1511
BseNI	ACTGG	4 cut(s)	555, 623, 1218, 1495
BseRI	GAGGAG	5 cut(s)	1272, 1275, 1278, 1281, 1537
BseSI	GKGCMC	2 cut(s)	300, 1115
BseXI	GCAGC	3 cut(s)	578, 731, 1664
Bsh1236I	CGCG	2 cut(s)	800, 2163
Bsh1285I	CGRYCG	1 cut(s)	324
BshFI	GGCC	2 cut(s)	319, 561
BsiEI	CGRYCG	1 cut(s)	324
BsiHKAI	GWGCWC	2 cut(s)	300, 1115
BsiSI	CCGG	3 cut(s)	184, 320, 820
BslFI	GGGAC	3 cut(s)	343, 1200, 1229
BslI	CCNNNNNNNGG	5 cut(s)	618, 1152, 1253, 1254, 1295
BsmAI	GTCTC	1 cut(s)	2077
BsmFI	GGGAC	3 cut(s)	343, 1200, 1229
BsnI	GGCC	2 cut(s)	319, 561
Bsp1286I	GDGCHC	2 cut(s)	300, 1115
Bsp13I	TCCGGA	1 cut(s)	819
Bsp1407I	TGTACA	2 cut(s)	1095, 1861
Bsp143I	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
Bsp1720I	GCTNAGC	1 cut(s)	536
Bsp68I	TCGCGA	1 cut(s)	2163
BspACI	CCGC	5 cut(s)	525, 663, 800, 1737, 1826
BspANI	GGCC	2 cut(s)	319, 561
BspCNI	CTCAG	3 cut(s)	361, 896, 1512
BspEI	TCCGGA	1 cut(s)	819
BspFNI	CGCG	2 cut(s)	800, 2163
BspLI	GGNNCC	1 cut(s)	1411
BspMAI	CTGCAG	1 cut(s)	1135
BspPI	GGATC	4 cut(s)	175, 560, 1404, 1417
BspTI	CTTAAG	1 cut(s)	1871
BsrDI	GCAATG	2 cut(s)	457, 2086
BsrFI	RCCGGY	1 cut(s)	319
BsrGI	TGTACA	2 cut(s)	1095, 1861
BsrI	ACTGG	4 cut(s)	555, 623, 1218, 1495
BssAI	RCCGGY	1 cut(s)	319
BssECI	CCNNGG	7 cut(s)	1038, 1047, 1059, 1262, 1389, 1413, 1552
BssMI	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
BssNI	GRCGYC	1 cut(s)	597
BssSI	CACGAG	1 cut(s)	2269
BssT1I	CCWWGG	4 cut(s)	1047, 1059, 1389, 1413
Bst2BI	CACGAG	1 cut(s)	2269
Bst2UI	CCWGG	1 cut(s)	1553
Bst6I	CTCTTC	1 cut(s)	662
BstACI	GRCGYC	1 cut(s)	597
BstAFI	CTTAAG	1 cut(s)	1871
BstAPI	GCANNNNNTGC	1 cut(s)	916
BstAUI	TGTACA	2 cut(s)	1095, 1861
BstBAI	YACGTR	1 cut(s)	1641
BstC8I	GCNNGC	3 cut(s)	529, 533, 1115
BstDEI	CTNAG	8 cut(s)	107, 219, 348, 536, 883, 1151, 1520, 2147
BstDSI	CCRYGG	1 cut(s)	1262
BstENI	CCTNNNNNAGG	2 cut(s)	1150, 1293
BstF5I	GGATG	4 cut(s)	501, 945, 2232, 2353
BstFNI	CGCG	2 cut(s)	800, 2163
BstHHI	GCGC	4 cut(s)	457, 785, 1069, 1108
BstKTI	GATC	9 cut(s)	56, 183, 498, 522, 568, 1412, 1529, 1607, 2254
BstMAI	GTCTC	1 cut(s)	2077
BstMBI	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
BstMCI	CGRYCG	1 cut(s)	324
BstMWI	GCNNNNNNNGC	5 cut(s)	537, 789, 916, 1049, 1114
BstNI	CCWGG	1 cut(s)	1553
BstNSI	RCATGY	6 cut(s)	28, 383, 531, 535, 1073, 1344
BstPAI	GACNNNNGTC	1 cut(s)	1986
BstSCI	CCNGG	1 cut(s)	1551
BstSFI	CTRYAG	1 cut(s)	1131
BstSLI	GKGCMC	2 cut(s)	300, 1115
BstUI	CGCG	2 cut(s)	800, 2163
BstV1I	GCAGC	3 cut(s)	578, 731, 1664
BstX2I	RGATCY	2 cut(s)	53, 1409
BstYI	RGATCY	2 cut(s)	53, 1409
BsuRI	GGCC	2 cut(s)	319, 561
BtgI	CCRYGG	1 cut(s)	1262
BtgZI	GCGATG	2 cut(s)	179, 2144
BtrI	CACGTC	1 cut(s)	429
BtsCI	GGATG	4 cut(s)	501, 945, 2232, 2353
BtsI	GCAGTG	2 cut(s)	1115, 1128
BtsIMutI	CAGTG	5 cut(s)	80, 96, 562, 1115, 1128
BtuMI	TCGCGA	1 cut(s)	2163
Cac8I	GCNNGC	3 cut(s)	529, 533, 1115
CfoI	GCGC	4 cut(s)	457, 785, 1069, 1108
Cfr10I	RCCGGY	1 cut(s)	319
Cfr13I	GGNCC	1 cut(s)	1120
CseI	GACGC	1 cut(s)	605
Csp6I	GTAC	8 cut(s)	622, 856, 880, 956, 1096, 1234, 1862, 2286
CviQI	GTAC	8 cut(s)	622, 856, 880, 956, 1096, 1234, 1862, 2286
DdeI	CTNAG	8 cut(s)	107, 219, 348, 536, 883, 1151, 1520, 2147
DpnI	GATC	9 cut(s)	55, 182, 497, 521, 567, 1411, 1528, 1606, 2253
DpnII	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
DriI	GACNNNNNGTC	1 cut(s)	340
EaeI	YGGCCR	1 cut(s)	559
Eam1104I	CTCTTC	1 cut(s)	662
Eam1105I	GACNNNNNGTC	1 cut(s)	340
EarI	CTCTTC	1 cut(s)	662
EciI	GGCGGA	2 cut(s)	652, 1752
Eco130I	CCWWGG	4 cut(s)	1047, 1059, 1389, 1413
Eco32I	GATATC	1 cut(s)	925
Eco47I	GGWCC	1 cut(s)	1120
Eco57I	CTGAAG	2 cut(s)	1886, 2280
EcoNI	CCTNNNNNAGG	2 cut(s)	1150, 1293
EcoO109I	RGGNCCY	1 cut(s)	1120
EcoRI	GAATTC	1 cut(s)	632
EcoRII	CCWGG	1 cut(s)	1551
EcoRV	GATATC	1 cut(s)	925
EcoT14I	CCWWGG	4 cut(s)	1047, 1059, 1389, 1413
EcoT22I	ATGCAT	1 cut(s)	533
ErhI	CCWWGG	4 cut(s)	1047, 1059, 1389, 1413
FaqI	GGGAC	3 cut(s)	343, 1200, 1229
FbaI	TGATCA	1 cut(s)	2251
FblI	GTMKAC	1 cut(s)	774
Fnu4HI	GCNGC	3 cut(s)	592, 745, 1653
FokI	GGATG	4 cut(s)	488, 932, 2239, 2360
Fsp4HI	GCNGC	3 cut(s)	592, 745, 1653
FspBI	CTAG	3 cut(s)	1649, 1916, 2232
FspI	TGCGCA	2 cut(s)	784, 1107
GlaI	GCGC	4 cut(s)	456, 784, 1068, 1107
GluI	GCNGC	3 cut(s)	592, 745, 1653
HaeIII	GGCC	2 cut(s)	319, 561
HapII	CCGG	3 cut(s)	184, 320, 820
HgaI	GACGC	1 cut(s)	605
HhaI	GCGC	4 cut(s)	457, 785, 1069, 1108
Hin1I	GRCGYC	1 cut(s)	597
Hin6I	GCGC	4 cut(s)	455, 783, 1067, 1106
HinP1I	GCGC	4 cut(s)	455, 783, 1067, 1106
HincII	GTYRAC	1 cut(s)	2025
HindII	GTYRAC	1 cut(s)	2025
HindIII	AAGCTT	3 cut(s)	1433, 1898, 1922
HinfI	GANTC	7 cut(s)	94, 604, 890, 1191, 1661, 2138, 2203
HpaI	GTTAAC	1 cut(s)	2025
HpaII	CCGG	3 cut(s)	184, 320, 820
HphI	GGTGA	5 cut(s)	336, 773, 919, 1027, 1757
Hpy166II	GTNNAC	8 cut(s)	191, 298, 775, 796, 839, 1113, 1862, 2025
Hpy188I	TCNGA	7 cut(s)	247, 472, 631, 886, 995, 1521, 1714
Hpy188III	TCNNGA	7 cut(s)	569, 820, 1533, 1991, 2082, 2114, 2162
Hpy8I	GTNNAC	8 cut(s)	191, 298, 775, 796, 839, 1113, 1862, 2025
Hpy99I	CGWCG	3 cut(s)	510, 599, 1494
HpyAV	CCTTC	3 cut(s)	166, 1937, 2255
HpyCH4IV	ACGT	6 cut(s)	356, 428, 765, 777, 1091, 1640
HpyF10VI	GCNNNNNNNGC	5 cut(s)	537, 789, 916, 1049, 1114
HpyF3I	CTNAG	8 cut(s)	107, 219, 348, 536, 883, 1151, 1520, 2147
HpySE526I	ACGT	6 cut(s)	356, 428, 765, 777, 1091, 1640
Hsp92I	GRCGYC	1 cut(s)	597
HspAI	GCGC	4 cut(s)	455, 783, 1067, 1106
Kpn2I	TCCGGA	1 cut(s)	819
Ksp22I	TGATCA	1 cut(s)	2251
KspAI	GTTAAC	1 cut(s)	2025
Kzo9I	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
Lsp1109I	GCAGC	3 cut(s)	578, 731, 1664
LweI	GCATC	2 cut(s)	110, 123
MaeI	CTAG	3 cut(s)	1649, 1916, 2232
MaeII	ACGT	6 cut(s)	356, 428, 765, 777, 1091, 1640
MaeIII	GTNAC	8 cut(s)	151, 342, 761, 1033, 1072, 1354, 1511, 2362
MalI	GATC	9 cut(s)	55, 182, 497, 521, 567, 1411, 1528, 1606, 2253
MboI	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
MfeI	CAATTG	2 cut(s)	194, 2069
MflI	RGATCY	2 cut(s)	53, 1409
MhlI	GDGCHC	2 cut(s)	300, 1115
MlyI	GAGTC	3 cut(s)	598, 899, 1670
Mph1103I	ATGCAT	1 cut(s)	533
MroI	TCCGGA	1 cut(s)	819
MslI	CAYNNNNRTG	2 cut(s)	431, 932
MspA1I	CMGCKG	1 cut(s)	1655
MspCI	CTTAAG	1 cut(s)	1871
MspI	CCGG	3 cut(s)	184, 320, 820
MspR9I	CCNGG	1 cut(s)	1553
MunI	CAATTG	2 cut(s)	194, 2069
MvaI	CCWGG	1 cut(s)	1553
MvnI	CGCG	2 cut(s)	800, 2163
MwoI	GCNNNNNNNGC	5 cut(s)	537, 789, 916, 1049, 1114
NdeII	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
NlaIV	GGNNCC	1 cut(s)	1411
NmuCI	GTSAC	4 cut(s)	342, 761, 1033, 1354
NruI	TCGCGA	1 cut(s)	2163
NsbI	TGCGCA	2 cut(s)	784, 1107
NsiI	ATGCAT	1 cut(s)	533
NspI	RCATGY	6 cut(s)	28, 383, 531, 535, 1073, 1344
PaeI	GCATGC	2 cut(s)	531, 535
PciI	ACATGT	1 cut(s)	379
PfeI	GAWTC	4 cut(s)	94, 1191, 2138, 2203
PkrI	GCNGC	3 cut(s)	593, 746, 1654
PleI	GAGTC	3 cut(s)	598, 898, 1669
PpsI	GAGTC	3 cut(s)	598, 898, 1669
Ppu21I	YACGTR	1 cut(s)	1641
PpuMI	RGGWCCY	1 cut(s)	1120
PscI	ACATGT	1 cut(s)	379
PshAI	GACNNNNGTC	1 cut(s)	1986
PshBI	ATTAAT	1 cut(s)	374
PsiI	TTATAA	2 cut(s)	146, 402
Psp5II	RGGWCCY	1 cut(s)	1120
Psp6I	CCWGG	1 cut(s)	1551
PspGI	CCWGG	1 cut(s)	1551
PspN4I	GGNNCC	1 cut(s)	1411
PspPI	GGNCC	1 cut(s)	1120
PspPPI	RGGWCCY	1 cut(s)	1120
PstI	CTGCAG	1 cut(s)	1135
PsuI	RGATCY	2 cut(s)	53, 1409
PvuII	CAGCTG	1 cut(s)	1655
RruI	TCGCGA	1 cut(s)	2163
RsaI	GTAC	8 cut(s)	623, 857, 881, 957, 1097, 1235, 1863, 2287
RsaNI	GTAC	8 cut(s)	622, 856, 880, 956, 1096, 1234, 1862, 2286
RseI	CAYNNNNRTG	2 cut(s)	431, 932
SatI	GCNGC	3 cut(s)	592, 745, 1653
Sau3AI	GATC	9 cut(s)	53, 180, 495, 519, 565, 1409, 1526, 1604, 2251
Sau96I	GGNCC	1 cut(s)	1120
SchI	GAGTC	3 cut(s)	598, 899, 1670
ScrFI	CCNGG	1 cut(s)	1553
SduI	GDGCHC	2 cut(s)	300, 1115
SfaNI	GCATC	2 cut(s)	110, 123
SfcI	CTRYAG	1 cut(s)	1131
SinI	GGWCC	1 cut(s)	1120
SmiMI	CAYNNNNRTG	2 cut(s)	431, 932
SmlI	CTYRAG	3 cut(s)	1293, 1871, 2112
SmoI	CTYRAG	3 cut(s)	1293, 1871, 2112
SphI	GCATGC	2 cut(s)	531, 535
SsiI	CCGC	5 cut(s)	525, 663, 800, 1737, 1826
SspI	AATATT	2 cut(s)	914, 1087
SspMI	CTAG	3 cut(s)	1649, 1916, 2232
StyD4I	CCNGG	1 cut(s)	1551
StyI	CCWWGG	4 cut(s)	1047, 1059, 1389, 1413
TaiI	ACGT	6 cut(s)	359, 431, 768, 780, 1094, 1643
TaqI	TCGA	3 cut(s)	508, 1603, 2081
TatI	WGTACW	2 cut(s)	1095, 1861
TfiI	GAWTC	4 cut(s)	94, 1191, 2138, 2203
TscAI	CASTG	5 cut(s)	87, 96, 562, 1115, 1135
TseFI	GTSAC	4 cut(s)	342, 761, 1033, 1354
TseI	GCWGC	3 cut(s)	591, 744, 1652
Tsp45I	GTSAC	4 cut(s)	342, 761, 1033, 1354
TspGWI	ACGGA	2 cut(s)	494, 1422
TspRI	CASTG	5 cut(s)	87, 96, 562, 1115, 1135
Vha464I	CTTAAG	1 cut(s)	1871
VneI	GTGCAC	2 cut(s)	296, 1111
VpaK11BI	GGWCC	1 cut(s)	1120
VspI	ATTAAT	1 cut(s)	374
XagI	CCTNNNNNAGG	2 cut(s)	1150, 1293
XapI	RAATTY	5 cut(s)	329, 632, 1205, 1595, 2118
XceI	RCATGY	6 cut(s)	28, 383, 531, 535, 1073, 1344
XmiI	GTMKAC	1 cut(s)	774
XspI	CTAG	3 cut(s)	1649, 1916, 2232
Zsp2I	ATGCAT	1 cut(s)	533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.