pycom01g14800

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020384
Physical Location & Seq
Forward (+)
14886087 .. 14887137
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGGTTATTCTCATTGCACAACAAAAACAATTTTTTTCAAAAAAACACAACACCAAACTAGCCCTAAACCGACTAAAACTTATCAATCATTTTTGTGGCCTAAGTACTTTTAATGAAGGGTTAAAATTCTATAAATCGATCCATTATTGCAAGTATGATGCACCAAACTTACGTTGCTTGAAAAATATCTCAAAGCTCTCTGTGTACATGGCAACCTCCAATCCAAGTGTTTGTTTGAAGCTCCTCATCGATACAAAGGGTCAACGAGTTCTCTTTGCTGAAGCTGGCAAAGACTTTGTGGATTTTCTCCTCACAATCCTGTCTTTGCCTCTTGGCACTGTCATCCGGCTCCTCTCCAAAGATGGCATGGTTGGCTCGTTAGGCAAGCTTTACGGCAGTGTCGAAAGCCTAAGCAGCACATACATGCAACCACACTTTAACAAGGAATCCCTCCTGAAACCTAAGGCAACAGCAACAATTGATGTTGGTGCTGATGTCCTTCATATGCTGACAATAGATGACTCCTCTGCTAAAAAGTCAATCTATGGTTGTACTAATTGTTGGGGCTACAGCAGCCGGCCAATCGTTGTAACCGACGACCCTAAAGCCACTTGTCCGACGTGCGGTGCAAGCATAAATACCAAGGCGACTTTTGTCCATCGATCCGAGGCTGAAGGAACAAAGTCCGGCGAGGGAGGGTATGTGAAAGGTGTTGTGACCTATATGATTATGGATGACCTGGAGGTGAAGCCTATGTCTACCATTTCAAGCGTAACCATGCTCAACACGTTCAACGTCAAGGATGTCGGTGCCCTTGAAGAGAAGGAGGTCCATCTTACCATGGAAGAGGGTGTCAAATTGCTGAGGGTATCATTGCAGTCAAACTTGGTTCTGACCACCGTGTTCCTTGATAAGAATGAAGCGTGTTCATTGCAGTCAAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

316

Amino Acids

34.64

Weight (kDa)

8.78

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 74 - 309 3.1e-45 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020384)

Species Orthologous Gene IDs
malus_domestica MD01G1121000.v1.1 MD01G1121100.v1.1
pyrus_communis pycom01g14770 pycom01g14790 pycom01g14800 pycom01g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 809
AccI GTMKAC 1 cut(s) 758
AciI CCGC 1 cut(s) 624
AclWI GGATC 2 cut(s) 133, 657
AcoI YGGCCR 1 cut(s) 578
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 2 cut(s) 300, 693
AfaI GTAC 3 cut(s) 106, 206, 553
AfiI CCNNNNNNNGG 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 786
AgsI TTSAA 6 cut(s) 39, 181, 238, 768, 793, 818
AjiI CACGTC 1 cut(s) 621
AjnI CCWGG 1 cut(s) 738
AluBI AGCT 4 cut(s) 196, 241, 284, 388
AluI AGCT 4 cut(s) 196, 241, 284, 388
AlwI GGATC 2 cut(s) 133, 657
AoxI GGCC 2 cut(s) 97, 578
ApeKI GCWGC 2 cut(s) 414, 573
ApoI RAATTY 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 829
AsuHPI GGTGA 1 cut(s) 757
AvaII GGWCC 1 cut(s) 829
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 814
BanI GGYRCC 1 cut(s) 809
BbvCI CCTCAGC 1 cut(s) 863
BbvI GCAGC 2 cut(s) 426, 585
BccI CCATC 3 cut(s) 356, 666, 840
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 740
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 568
BisI GCNGC 2 cut(s) 415, 574
BlsI GCNGC 2 cut(s) 416, 575
BmcAI AGTACT 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 740
Bme18I GGWCC 1 cut(s) 829
BmgBI CACGTC 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 829
BmiI GGNNCC 2 cut(s) 350, 811
BmrFI CCNGG 1 cut(s) 740
BmsI GCATC 1 cut(s) 148
BoxI GACNNNNGTC 1 cut(s) 653
BpmI CTGGAG 1 cut(s) 761
Bpu10I CCTNAGC 2 cut(s) 410, 863
Bsa29I ATCGAT 3 cut(s) 137, 249, 661
BsaJI CCNNGG 3 cut(s) 642, 666, 840
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse118I RCCGGY 1 cut(s) 576
Bse21I CCTNAGG 1 cut(s) 462
Bse3DI GCAATG 3 cut(s) 12, 872, 929
BseBI CCWGG 1 cut(s) 740
BseCI ATCGAT 3 cut(s) 137, 249, 661
BseDI CCNNGG 3 cut(s) 642, 666, 840
BseGI GGATG 3 cut(s) 342, 739, 808
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 3 cut(s) 12, 872, 929
BseMII CTCAG 1 cut(s) 854
BseRI GAGGAG 4 cut(s) 233, 299, 341, 514
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 2 cut(s) 426, 585
BshFI GGCC 2 cut(s) 99, 580
BshNI GGYRCC 1 cut(s) 809
BshVI ATCGAT 3 cut(s) 137, 249, 661
BsiSI CCGG 3 cut(s) 346, 577, 687
BslI CCNNNNNNNGG 1 cut(s) 623
BsnI GGCC 2 cut(s) 99, 580
Bsp1286I GDGCHC 1 cut(s) 814
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 138, 662
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 1 cut(s) 624
BspANI GGCC 2 cut(s) 99, 580
BspCNI CTCAG 1 cut(s) 855
BspDI ATCGAT 3 cut(s) 137, 249, 661
BspLI GGNNCC 2 cut(s) 350, 811
BspPI GGATC 2 cut(s) 133, 657
BspT107I GGYRCC 1 cut(s) 809
BsrDI GCAATG 3 cut(s) 12, 872, 929
BsrFI RCCGGY 1 cut(s) 576
BsrGI TGTACA 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 576
BssECI CCNNGG 3 cut(s) 642, 666, 840
BssMI GATC 2 cut(s) 138, 662
BssT1I CCWWGG 2 cut(s) 642, 840
Bst2UI CCWGG 1 cut(s) 740
Bst4CI ACNGT 2 cut(s) 340, 901
Bst6I CTCTTC 2 cut(s) 813, 840
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 4 cut(s) 286, 386, 578, 631
BstDEI CTNAG 4 cut(s) 101, 410, 462, 863
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 3 cut(s) 342, 739, 808
BstKTI GATC 2 cut(s) 141, 665
BstMBI GATC 2 cut(s) 138, 662
BstMWI GCNNNNNNNGC 5 cut(s) 372, 381, 414, 573, 630
BstNI CCWGG 1 cut(s) 740
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 738
BstSFI CTRYAG 1 cut(s) 568
BstSLI GKGCMC 1 cut(s) 814
BstV1I GCAGC 2 cut(s) 426, 585
Bsu15I ATCGAT 3 cut(s) 137, 249, 661
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 2 cut(s) 99, 580
BsuTUI ATCGAT 3 cut(s) 137, 249, 661
BtgI CCRYGG 1 cut(s) 840
BtrI CACGTC 1 cut(s) 621
BtsCI GGATG 3 cut(s) 342, 739, 808
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 336, 403
Cac8I GCNNGC 4 cut(s) 286, 386, 578, 631
Cfr10I RCCGGY 1 cut(s) 576
Cfr13I GGNCC 1 cut(s) 829
ClaI ATCGAT 3 cut(s) 137, 249, 661
Csp6I GTAC 3 cut(s) 105, 205, 552
CviAII CATG 5 cut(s) 208, 367, 424, 778, 841
CviQI GTAC 3 cut(s) 105, 205, 552
DdeI CTNAG 4 cut(s) 101, 410, 462, 863
DpnI GATC 2 cut(s) 140, 664
DpnII GATC 2 cut(s) 138, 662
EaeI YGGCCR 1 cut(s) 578
Eam1104I CTCTTC 2 cut(s) 813, 840
EarI CTCTTC 2 cut(s) 813, 840
Eco130I CCWWGG 2 cut(s) 642, 840
Eco47I GGWCC 1 cut(s) 829
Eco57I CTGAAG 2 cut(s) 300, 693
Eco81I CCTNAGG 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 738
EcoT14I CCWWGG 2 cut(s) 642, 840
ErhI CCWWGG 2 cut(s) 642, 840
FaeI CATG 5 cut(s) 211, 370, 427, 781, 844
FatI CATG 5 cut(s) 207, 366, 423, 777, 840
FauNDI CATATG 1 cut(s) 504
FblI GTMKAC 1 cut(s) 758
Fnu4HI GCNGC 2 cut(s) 415, 574
FokI GGATG 3 cut(s) 329, 746, 815
Fsp4HI GCNGC 2 cut(s) 415, 574
FspBI CTAG 1 cut(s) 59
GluI GCNGC 2 cut(s) 415, 574
GsuI CTGGAG 1 cut(s) 761
HaeIII GGCC 2 cut(s) 99, 580
HapII CCGG 3 cut(s) 346, 577, 687
Hin1II CATG 5 cut(s) 211, 370, 427, 781, 844
HincII GTYRAC 1 cut(s) 263
HindII GTYRAC 1 cut(s) 263
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 2 cut(s) 446, 521
HpaII CCGG 3 cut(s) 346, 577, 687
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 3 cut(s) 205, 263, 759
Hpy188I TCNGA 3 cut(s) 618, 667, 894
Hpy188III TCNNGA 1 cut(s) 454
Hpy8I GTNNAC 3 cut(s) 205, 263, 759
Hpy99I CGWCG 2 cut(s) 599, 622
HpyAV CCTTC 4 cut(s) 110, 509, 668, 817
HpyCH4III ACNGT 2 cut(s) 340, 901
HpyCH4IV ACGT 4 cut(s) 172, 620, 788, 795
HpyCH4V TGCA 7 cut(s) 17, 150, 161, 427, 629, 877, 934
HpyF10VI GCNNNNNNNGC 5 cut(s) 372, 381, 414, 573, 630
HpyF3I CTNAG 4 cut(s) 101, 410, 462, 863
HpySE526I ACGT 4 cut(s) 172, 620, 788, 795
Hsp92II CATG 5 cut(s) 211, 370, 427, 781, 844
KroI GCCGGC 1 cut(s) 576
KroNI GCCGGC 1 cut(s) 578
Kzo9I GATC 2 cut(s) 138, 662
LmnI GCTCC 2 cut(s) 246, 354
LpnPI CCDG 8 cut(s) 270, 332, 359, 467, 590, 700, 725, 752
Lsp1109I GCAGC 2 cut(s) 426, 585
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 59
MaeII ACGT 4 cut(s) 172, 620, 788, 795
MaeIII GTNAC 3 cut(s) 589, 715, 772
MalI GATC 2 cut(s) 140, 664
MboI GATC 2 cut(s) 138, 662
MboII GAAGA 2 cut(s) 830, 857
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 6 cut(s) 29, 125, 477, 556, 857, 940
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 641
MroNI GCCGGC 1 cut(s) 576
MseI TTAA 3 cut(s) 111, 122, 438
MslI CAYNNNNRTG 2 cut(s) 93, 422
MspI CCGG 3 cut(s) 346, 577, 687
MspR9I CCNGG 1 cut(s) 740
MunI CAATTG 1 cut(s) 477
MvaI CCWGG 1 cut(s) 740
MwoI GCNNNNNNNGC 5 cut(s) 372, 381, 414, 573, 630
NaeI GCCGGC 1 cut(s) 578
NcoI CCATGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 504
NdeII GATC 2 cut(s) 138, 662
NgoMIV GCCGGC 1 cut(s) 576
NlaIII CATG 5 cut(s) 211, 370, 427, 781, 844
NlaIV GGNNCC 2 cut(s) 350, 811
NmuCI GTSAC 1 cut(s) 715
NspI RCATGY 1 cut(s) 427
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 591
PdiI GCCGGC 1 cut(s) 578
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 2 cut(s) 416, 575
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
PshAI GACNNNNGTC 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 738
PspGI CCWGG 1 cut(s) 738
PspN4I GGNNCC 2 cut(s) 350, 811
PspPI GGNCC 1 cut(s) 829
RsaI GTAC 3 cut(s) 106, 206, 553
RsaNI GTAC 3 cut(s) 105, 205, 552
RseI CAYNNNNRTG 2 cut(s) 93, 422
SaqAI TTAA 3 cut(s) 111, 122, 438
SatI GCNGC 2 cut(s) 415, 574
Sau3AI GATC 2 cut(s) 138, 662
Sau96I GGNCC 1 cut(s) 829
ScaI AGTACT 1 cut(s) 106
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 1 cut(s) 740
SduI GDGCHC 1 cut(s) 814
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 568
SinI GGWCC 1 cut(s) 829
SmiMI CAYNNNNRTG 2 cut(s) 93, 422
Sse9I AATT 6 cut(s) 29, 125, 477, 556, 857, 940
SsiI CCGC 1 cut(s) 624
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 738
StyI CCWWGG 2 cut(s) 642, 840
TaaI ACNGT 2 cut(s) 340, 901
TaiI ACGT 4 cut(s) 175, 623, 791, 798
TaqI TCGA 4 cut(s) 137, 249, 402, 661
TasI AATT 6 cut(s) 29, 125, 477, 556, 857, 940
TatI WGTACW 3 cut(s) 104, 204, 551
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 3 cut(s) 111, 122, 438
Tru9I TTAA 3 cut(s) 111, 122, 438
TscAI CASTG 2 cut(s) 343, 403
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 2 cut(s) 414, 573
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 4 cut(s) 129, 491, 918, 933
TspRI CASTG 2 cut(s) 343, 403
VpaK11BI GGWCC 1 cut(s) 829
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 758
XspI CTAG 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.