pycom01g14810

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020384
Physical Location & Seq
Forward (+)
14910010 .. 14910857
848 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGGCAAACCCCAAGCCAAGTATTTGCTTGAAGCTCCTTGTCGACACAAAGCGTCAACGAGTTCTCTTTGCTGAAGCTGGCAAAGACTTTGTGGACTTTCTCTTCACACTCCTGTCTTTGCCCATTGGCACTGTCATCGGGCTCCTTTCCAAAGATGGCATGGTTGGCTCGTTAGGCAAACTTTACGGCAGTGTCAAGAGCCTAAGCAGCATATACATGCAACCTTACTTTAACAAGGAATCCCTCCTGAAATCTAAGGCAACAGCAGCGATTGGTGTTGGTGCCGATGTCCTTCATATGCTGATGTTAGACGACTCCACTGCTGAAAAGTCATTCTATTATTGTGATTGTCTTAGTCGCCGCCAACAATTTTACTACAACGGATCCAGTGGAAATCCAACCGTTGTAACGGACAACCCTAAAGCCATTTGTCCACACTGCATTTCAAGCATAAATACCAAGGCGACTTTTGTCCATGGATCCGCTGCTGAAAGAGGAACGTCCGGCGAGGGAGGGTATGTGAAAGGTGTTGTCACGTATATGATTATGGATGACCTGGAAGTGCAGCCTATGTCTACCATTTCAAGCATAACCATGCTTAACAGGTTCAACGTCAGGGATGTCGGTGCCCTTGAAGAGAAGGTGGTCCATCTTACCATGGAAGAGGGTCTCAAATTGCTGAGGGCATCGTTGCACTCAAACTTGGTCCTGACCACCGTGTTCCTTGGTACGAAGGAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

248

Amino Acids

26.88

Weight (kDa)

8.2

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 245 8.8e-40 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020384)

Species Orthologous Gene IDs
malus_domestica MD01G1121000.v1.1 MD01G1121100.v1.1
pyrus_communis pycom01g14770 pycom01g14790 pycom01g14800 pycom01g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 281, 626
AccI GTMKAC 2 cut(s) 42, 575
AciI CCGC 2 cut(s) 361, 483
AclWI GGATC 4 cut(s) 378, 391, 474, 487
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 1 cut(s) 730
AgsI TTSAA 5 cut(s) 31, 447, 585, 610, 635
AjnI CCWGG 1 cut(s) 555
AluBI AGCT 2 cut(s) 34, 77
AluI AGCT 2 cut(s) 34, 77
Alw26I GTCTC 1 cut(s) 674
AlwI GGATC 4 cut(s) 378, 391, 474, 487
ApeKI GCWGC 4 cut(s) 207, 266, 485, 565
AspS9I GGNCC 2 cut(s) 646, 706
AvaII GGWCC 2 cut(s) 646, 706
BaeGI GKGCMC 1 cut(s) 631
BamHI GGATCC 2 cut(s) 383, 479
BanI GGYRCC 2 cut(s) 281, 626
BanII GRGCYC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 680
BbvI GCAGC 4 cut(s) 219, 278, 472, 577
BccI CCATC 2 cut(s) 149, 657
BceAI ACGGC 1 cut(s) 202
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 302, 336
BciT130I CCWGG 1 cut(s) 557
BcoDI GTCTC 1 cut(s) 674
BisI GCNGC 5 cut(s) 208, 267, 361, 486, 566
BlsI GCNGC 5 cut(s) 209, 268, 362, 487, 567
Bme1390I CCNGG 1 cut(s) 557
Bme18I GGWCC 2 cut(s) 646, 706
BmgT120I GGNCC 2 cut(s) 646, 706
BmiI GGNNCC 5 cut(s) 143, 283, 385, 481, 628
BmrFI CCNGG 1 cut(s) 557
BmsI GCATC 1 cut(s) 695
BoxI GACNNNNGTC 1 cut(s) 470
Bpu10I CCTNAGC 2 cut(s) 203, 680
BsaAI YACGTR 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 4 cut(s) 459, 475, 657, 724
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bse1I ACTGG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 4 cut(s) 459, 475, 657, 724
BseGI GGATG 2 cut(s) 556, 625
BseMII CTCAG 1 cut(s) 671
BseNI ACTGG 1 cut(s) 387
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 4 cut(s) 219, 278, 472, 577
BsgI GTGCAG 1 cut(s) 584
BshNI GGYRCC 2 cut(s) 281, 626
BsiSI CCGG 1 cut(s) 504
BsmAI GTCTC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 674
Bsp1286I GDGCHC 2 cut(s) 144, 631
Bsp143I GATC 2 cut(s) 383, 479
Bsp19I CCATGG 2 cut(s) 475, 657
BspACI CCGC 2 cut(s) 361, 483
BspCNI CTCAG 1 cut(s) 672
BspLI GGNNCC 5 cut(s) 143, 283, 385, 481, 628
BspPI GGATC 4 cut(s) 378, 391, 474, 487
BspT107I GGYRCC 2 cut(s) 281, 626
BspTNI GGTCTC 1 cut(s) 674
BsrI ACTGG 1 cut(s) 387
BssECI CCNNGG 4 cut(s) 459, 475, 657, 724
BssMI GATC 2 cut(s) 383, 479
BssT1I CCWWGG 4 cut(s) 459, 475, 657, 724
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 3 cut(s) 133, 403, 718
Bst6I CTCTTC 3 cut(s) 107, 630, 657
BstBAI YACGTR 1 cut(s) 537
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 4 cut(s) 203, 255, 353, 680
BstDSI CCRYGG 2 cut(s) 475, 657
BstF5I GGATG 2 cut(s) 556, 625
BstKTI GATC 2 cut(s) 386, 482
BstMAI GTCTC 1 cut(s) 674
BstMBI GATC 2 cut(s) 383, 479
BstMWI GCNNNNNNNGC 5 cut(s) 165, 174, 207, 266, 447
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 555
BstSLI GKGCMC 1 cut(s) 631
BstV1I GCAGC 4 cut(s) 219, 278, 472, 577
BstX2I RGATCY 2 cut(s) 383, 479
BstYI RGATCY 2 cut(s) 383, 479
BtgI CCRYGG 2 cut(s) 475, 657
BtsCI GGATG 2 cut(s) 556, 625
BtsI GCAGTG 3 cut(s) 196, 318, 436
BtsIMutI CAGTG 5 cut(s) 129, 196, 318, 394, 436
Cac8I GCNNGC 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 646, 706
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 729
CviAII CATG 5 cut(s) 160, 217, 476, 595, 658
CviJI RGCY 8 cut(s) 16, 34, 77, 142, 168, 201, 425, 568
CviKI_1 RGCY 8 cut(s) 16, 34, 77, 142, 168, 201, 425, 568
CviQI GTAC 1 cut(s) 729
DdeI CTNAG 4 cut(s) 203, 255, 353, 680
DpnI GATC 2 cut(s) 385, 481
DpnII GATC 2 cut(s) 383, 479
Eam1104I CTCTTC 3 cut(s) 107, 630, 657
EarI CTCTTC 3 cut(s) 107, 630, 657
Eco130I CCWWGG 4 cut(s) 459, 475, 657, 724
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 674
Eco47I GGWCC 2 cut(s) 646, 706
Eco57I CTGAAG 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 4 cut(s) 459, 475, 657, 724
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 4 cut(s) 459, 475, 657, 724
FaeI CATG 5 cut(s) 163, 220, 479, 598, 661
FatI CATG 5 cut(s) 159, 216, 475, 594, 657
FauNDI CATATG 1 cut(s) 297
FblI GTMKAC 2 cut(s) 42, 575
Fnu4HI GCNGC 5 cut(s) 208, 267, 361, 486, 566
FokI GGATG 2 cut(s) 563, 632
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 5 cut(s) 208, 267, 361, 486, 566
GluI GCNGC 5 cut(s) 208, 267, 361, 486, 566
HapII CCGG 1 cut(s) 504
HgaI GACGC 1 cut(s) 41
Hin1II CATG 5 cut(s) 163, 220, 479, 598, 661
HincII GTYRAC 2 cut(s) 43, 56
HindII GTYRAC 2 cut(s) 43, 56
HinfI GANTC 2 cut(s) 239, 314
HpaII CCGG 1 cut(s) 504
Hpy166II GTNNAC 5 cut(s) 43, 56, 94, 434, 576
Hpy188III TCNNGA 3 cut(s) 196, 247, 709
Hpy8I GTNNAC 5 cut(s) 43, 56, 94, 434, 576
HpyAV CCTTC 3 cut(s) 302, 634, 727
HpyCH4III ACNGT 3 cut(s) 133, 403, 718
HpyCH4IV ACGT 3 cut(s) 500, 536, 612
HpyCH4V TGCA 4 cut(s) 220, 441, 565, 694
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 174, 207, 266, 447
HpyF3I CTNAG 4 cut(s) 203, 255, 353, 680
HpySE526I ACGT 3 cut(s) 500, 536, 612
Hsp92II CATG 5 cut(s) 163, 220, 479, 598, 661
Kzo9I GATC 2 cut(s) 383, 479
LmnI GCTCC 2 cut(s) 39, 147
Lsp1109I GCAGC 4 cut(s) 219, 278, 472, 577
LweI GCATC 1 cut(s) 695
MaeII ACGT 3 cut(s) 500, 536, 612
MaeIII GTNAC 2 cut(s) 406, 532
MalI GATC 2 cut(s) 385, 481
MboI GATC 2 cut(s) 383, 479
MboII GAAGA 3 cut(s) 94, 647, 674
MflI RGATCY 2 cut(s) 383, 479
MhlI GDGCHC 2 cut(s) 144, 631
MluCI AATT 2 cut(s) 368, 674
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 422
MnlI CCTC 6 cut(s) 254, 488, 502, 506, 658, 675
MseI TTAA 2 cut(s) 231, 600
MslI CAYNNNNRTG 3 cut(s) 215, 539, 593
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 557
MvaI CCWGG 1 cut(s) 557
MwoI GCNNNNNNNGC 5 cut(s) 165, 174, 207, 266, 447
NcoI CCATGG 2 cut(s) 475, 657
NdeI CATATG 1 cut(s) 297
NdeII GATC 2 cut(s) 383, 479
NlaIII CATG 5 cut(s) 163, 220, 479, 598, 661
NlaIV GGNNCC 5 cut(s) 143, 283, 385, 481, 628
NmuCI GTSAC 1 cut(s) 532
NspI RCATGY 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 737
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 5 cut(s) 209, 268, 362, 487, 567
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 537
PshAI GACNNNNGTC 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 5 cut(s) 143, 283, 385, 481, 628
PspPI GGNCC 2 cut(s) 646, 706
PsuI RGATCY 2 cut(s) 383, 479
RsaI GTAC 1 cut(s) 730
RsaNI GTAC 1 cut(s) 729
RseI CAYNNNNRTG 3 cut(s) 215, 539, 593
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 231, 600
SatI GCNGC 5 cut(s) 208, 267, 361, 486, 566
Sau3AI GATC 2 cut(s) 383, 479
Sau96I GGNCC 2 cut(s) 646, 706
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 557
SduI GDGCHC 2 cut(s) 144, 631
SfaNI GCATC 1 cut(s) 695
SinI GGWCC 2 cut(s) 646, 706
SmiMI CAYNNNNRTG 3 cut(s) 215, 539, 593
Sse9I AATT 2 cut(s) 368, 674
SsiI CCGC 2 cut(s) 361, 483
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 4 cut(s) 459, 475, 657, 724
TaaI ACNGT 3 cut(s) 133, 403, 718
TaiI ACGT 3 cut(s) 503, 539, 615
TaqI TCGA 1 cut(s) 42
TasI AATT 2 cut(s) 368, 674
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 231, 600
Tru9I TTAA 2 cut(s) 231, 600
TscAI CASTG 5 cut(s) 136, 196, 325, 394, 443
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 4 cut(s) 207, 266, 485, 565
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 1 cut(s) 284
TspGWI ACGGA 2 cut(s) 396, 425
TspRI CASTG 5 cut(s) 136, 196, 325, 394, 443
VpaK11BI GGWCC 2 cut(s) 646, 706
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XmiI GTMKAC 2 cut(s) 42, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.