pycom01g14970

2-oxoglutarate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0024208
Physical Location & Seq
Forward (+)
15000280 .. 15001137
858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGTCGTGGTTTAGAGCTGGGTCTGGCGTGGCAAAGCTCGCCATTAGGAGAAGTTTATCGAATAGCGGGTCCTATGCGGCTAGGAGGCGGGTTCTTCCATCCCAAAGCCGAGACTTTCACACCACAATACCTAAATCGAAGGCTGCCCCGGTTCCACGACCTGTACCCCTCTCTAGGCTAACTGATAGTTTCTTAGATGGAACCAGCAGTGTTTATTTGGAGGGGCTTCAGAGGGCTTGGGAAGCCGACCCCAATAGCGTTGATGAGTCGTGGGACAATTTCTTTAGGAATTTTGTGGGTCAGGCCTCTACCTCGCCTGGAATTTCGGGGCAGACCATTCAAGAGAGTATGCGTCTGTTGTTGCTTGTGAGGGCATACCAGGTCAATGGTCATATGAAAGCTAAATTGGACCCTTTGGGTTTGGAAGAGAGAGAAATCCCTGATGATCTTGACCCTGCTCTATATGGGTTCACTGAGGCTGATCTTGATAGAGAGTTCTTTTTGGGGGTCTGGAGGATGGCTGGGTTTTTGTCTGAGAACCGTCCTGTGCAGACCCTTAGGTCCATATTGACCCGGCTTGAGCAGGCTTACTGTGGGACCATTGGGTATGAGTATATGCACATGATCGATCGTGAGAGATGTAACTGGTTGAGAGACAAGATTGAGACTCCTACATCAATGCAATATAATAGGCAGCGTCGTGAGGTTATTCTTGATAGGCTTATTTGGAGTACACAGTTTGAGAACTTCTTAGCCACTAAGTGGACAGCGGCAAAGAGGTTTGGGCTTGAAGGGGGAGAAACTCTTATTCCTGGCATGAAGGAGATGTTTGATAGGTCTGCTCGATCTCGGGGTTGA

Protein Analysis

286

Amino Acids

32.6

Weight (kDa)

9.09

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxogl_dehyd_N PF16078 62 - 99 7.3e-15 2-oxoglutarate dehydrogenase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024208)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g21781
pyrus_communis pycom01g14970
rosa_multiflora Rmu_co8298685.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 559
AccB7I CCANNNNNTGG 1 cut(s) 762
AciI CCGC 4 cut(s) 66, 77, 88, 770
AcsI RAATTY 2 cut(s) 289, 321
AcuI CTGAAG 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 762
AfaI GTAC 2 cut(s) 165, 733
AfiI CCNNNNNNNGG 2 cut(s) 174, 762
AgsI TTSAA 2 cut(s) 341, 791
AjnI CCWGG 3 cut(s) 316, 378, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AluBI AGCT 3 cut(s) 17, 37, 401
AluI AGCT 3 cut(s) 17, 37, 401
Alw26I GTCTC 3 cut(s) 105, 648, 659
Ama87I CYCGRG 1 cut(s) 849
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 143, 694
ApoI RAATTY 2 cut(s) 289, 321
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspS9I GGNCC 4 cut(s) 69, 409, 561, 597
AsuC2I CCSGG 2 cut(s) 149, 574
AvaI CYCGRG 1 cut(s) 849
AvaII GGWCC 4 cut(s) 69, 409, 561, 597
AxyI CCTNAGG 1 cut(s) 557
BbvI GCAGC 2 cut(s) 130, 706
BccI CCATC 3 cut(s) 106, 191, 511
BciT130I CCWGG 3 cut(s) 318, 380, 813
BcnI CCSGG 2 cut(s) 149, 574
BcoDI GTCTC 3 cut(s) 105, 648, 659
BfaI CTAG 2 cut(s) 81, 174
BisI GCNGC 4 cut(s) 78, 144, 695, 771
BlsI GCNGC 4 cut(s) 79, 145, 696, 772
Bme1390I CCNGG 5 cut(s) 149, 318, 380, 574, 813
Bme18I GGWCC 4 cut(s) 69, 409, 561, 597
BmeT110I CYCGRG 1 cut(s) 849
BmgT120I GGNCC 4 cut(s) 69, 409, 561, 597
BmiI GGNNCC 5 cut(s) 70, 153, 202, 411, 598
BmrFI CCNGG 5 cut(s) 149, 318, 380, 574, 813
BpmI CTGGAG 1 cut(s) 532
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 2 cut(s) 149, 574
Bsa29I ATCGAT 1 cut(s) 627
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 762
Bse1I ACTGG 1 cut(s) 650
Bse21I CCTNAGG 1 cut(s) 557
BseBI CCWGG 3 cut(s) 318, 380, 813
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 2 cut(s) 98, 522
BseLI CCNNNNNNNGG 2 cut(s) 174, 762
BseMII CTCAG 2 cut(s) 465, 525
BseNI ACTGG 1 cut(s) 650
BseXI GCAGC 2 cut(s) 130, 706
BseYI CCCAGC 2 cut(s) 17, 521
BsgI GTGCAG 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 631
BshFI GGCC 1 cut(s) 305
BshVI ATCGAT 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 631
BsiHKCI CYCGRG 1 cut(s) 849
BsiSI CCGG 2 cut(s) 149, 574
BslFI GGGAC 2 cut(s) 287, 610
BslI CCNNNNNNNGG 2 cut(s) 174, 762
BsmAI GTCTC 3 cut(s) 105, 648, 659
BsmFI GGGAC 2 cut(s) 287, 610
BsnI GGCC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 849
Bsp143I GATC 5 cut(s) 445, 481, 624, 628, 845
BspACI CCGC 4 cut(s) 66, 77, 88, 770
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 466, 526
BspDI ATCGAT 1 cut(s) 627
BspLI GGNNCC 5 cut(s) 70, 153, 202, 411, 598
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 5 cut(s) 445, 481, 624, 628, 845
Bst2UI CCWGG 3 cut(s) 318, 380, 813
Bst4CI ACNGT 3 cut(s) 542, 593, 738
Bst6I CTCTTC 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 39, 585
BstDEI CTNAG 6 cut(s) 193, 474, 534, 557, 751, 759
BstF5I GGATG 2 cut(s) 98, 522
BstKTI GATC 5 cut(s) 448, 484, 627, 631, 848
BstMAI GTCTC 3 cut(s) 105, 648, 659
BstMBI GATC 5 cut(s) 445, 481, 624, 628, 845
BstMCI CGRYCG 1 cut(s) 631
BstMWI GCNNNNNNNGC 2 cut(s) 38, 242
BstNI CCWGG 3 cut(s) 318, 380, 813
BstSCI CCNGG 5 cut(s) 147, 316, 378, 572, 811
BstV1I GCAGC 2 cut(s) 130, 706
BstXI CCANNNNNNTGG 1 cut(s) 386
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 1 cut(s) 557
BsuRI GGCC 1 cut(s) 305
BsuTUI ATCGAT 1 cut(s) 627
BtsCI GGATG 2 cut(s) 98, 522
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 471
Cac8I GCNNGC 2 cut(s) 39, 585
Cfr13I GGNCC 4 cut(s) 69, 409, 561, 597
ClaI ATCGAT 1 cut(s) 627
CseI GACGC 2 cut(s) 341, 686
CsiI ACCWGGT 1 cut(s) 378
Csp6I GTAC 2 cut(s) 164, 732
CviAII CATG 2 cut(s) 622, 817
CviQI GTAC 2 cut(s) 164, 732
DdeI CTNAG 6 cut(s) 193, 474, 534, 557, 751, 759
DpnI GATC 5 cut(s) 447, 483, 626, 630, 847
DpnII GATC 5 cut(s) 445, 481, 624, 628, 845
DraIII CACNNNGTG 1 cut(s) 762
DrdI GACNNNNNNGTC 1 cut(s) 559
DseDI GACNNNNNNGTC 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 305
Eco47I GGWCC 4 cut(s) 69, 409, 561, 597
Eco57I CTGAAG 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 557
Eco88I CYCGRG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 3 cut(s) 316, 378, 811
FaeI CATG 2 cut(s) 625, 820
FaqI GGGAC 2 cut(s) 287, 610
FatI CATG 2 cut(s) 621, 816
FauI CCCGC 2 cut(s) 59, 81
FauNDI CATATG 1 cut(s) 393
Fnu4HI GCNGC 4 cut(s) 78, 144, 695, 771
FokI GGATG 2 cut(s) 85, 529
Fsp4HI GCNGC 4 cut(s) 78, 144, 695, 771
FspBI CTAG 2 cut(s) 81, 174
GluI GCNGC 4 cut(s) 78, 144, 695, 771
GsaI CCCAGC 2 cut(s) 21, 525
GsuI CTGGAG 1 cut(s) 532
HaeIII GGCC 1 cut(s) 305
HapII CCGG 2 cut(s) 149, 574
HgaI GACGC 2 cut(s) 341, 686
Hin1II CATG 2 cut(s) 625, 820
HinfI GANTC 2 cut(s) 266, 667
HpaII CCGG 2 cut(s) 149, 574
Hpy166II GTNNAC 3 cut(s) 471, 734, 765
Hpy188I TCNGA 2 cut(s) 231, 535
Hpy188III TCNNGA 7 cut(s) 341, 449, 485, 511, 632, 701, 713
Hpy8I GTNNAC 3 cut(s) 471, 734, 765
Hpy99I CGWCG 1 cut(s) 702
HpyAV CCTTC 3 cut(s) 133, 785, 814
HpyCH4III ACNGT 3 cut(s) 542, 593, 738
HpyCH4V TGCA 3 cut(s) 550, 619, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 242
HpyF3I CTNAG 6 cut(s) 193, 474, 534, 557, 751, 759
Hsp92II CATG 2 cut(s) 625, 820
Kzo9I GATC 5 cut(s) 445, 481, 624, 628, 845
Lsp1109I GCAGC 2 cut(s) 130, 706
MabI ACCWGGT 1 cut(s) 378
MaeI CTAG 2 cut(s) 81, 174
MaeIII GTNAC 1 cut(s) 641
MalI GATC 5 cut(s) 447, 483, 626, 630, 847
MboI GATC 5 cut(s) 445, 481, 624, 628, 845
MboII GAAGA 2 cut(s) 86, 437
MluCI AATT 4 cut(s) 277, 289, 321, 404
MlyI GAGTC 2 cut(s) 275, 661
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 2 cut(s) 149, 574
MspR9I CCNGG 5 cut(s) 149, 318, 380, 574, 813
MvaI CCWGG 3 cut(s) 318, 380, 813
MwoI GCNNNNNNNGC 2 cut(s) 38, 242
NciI CCSGG 2 cut(s) 149, 574
NdeI CATATG 1 cut(s) 393
NdeII GATC 5 cut(s) 445, 481, 624, 628, 845
NlaIII CATG 2 cut(s) 625, 820
NlaIV GGNNCC 5 cut(s) 70, 153, 202, 411, 598
NmeAIII GCCGAG 1 cut(s) 134
PceI AGGCCT 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 762
PkrI GCNGC 4 cut(s) 79, 145, 696, 772
Ple19I CGATCG 1 cut(s) 631
PleI GAGTC 2 cut(s) 274, 661
PpsI GAGTC 2 cut(s) 274, 661
PpuMI RGGWCCY 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 316, 378, 811
PspFI CCCAGC 2 cut(s) 17, 521
PspGI CCWGG 3 cut(s) 316, 378, 811
PspN4I GGNNCC 5 cut(s) 70, 153, 202, 411, 598
PspPI GGNCC 4 cut(s) 69, 409, 561, 597
PspPPI RGGWCCY 1 cut(s) 69
PvuI CGATCG 1 cut(s) 631
RsaI GTAC 2 cut(s) 165, 733
RsaNI GTAC 2 cut(s) 164, 732
SatI GCNGC 4 cut(s) 78, 144, 695, 771
Sau3AI GATC 5 cut(s) 445, 481, 624, 628, 845
Sau96I GGNCC 4 cut(s) 69, 409, 561, 597
SchI GAGTC 2 cut(s) 275, 661
ScrFI CCNGG 5 cut(s) 149, 318, 380, 574, 813
SexAI ACCWGGT 1 cut(s) 378
SinI GGWCC 4 cut(s) 69, 409, 561, 597
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 4 cut(s) 277, 289, 321, 404
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 4 cut(s) 66, 77, 88, 770
SspMI CTAG 2 cut(s) 81, 174
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 5 cut(s) 147, 316, 378, 572, 811
TaaI ACNGT 3 cut(s) 542, 593, 738
TaqI TCGA 4 cut(s) 59, 137, 627, 844
TasI AATT 4 cut(s) 277, 289, 321, 404
TatI WGTACW 1 cut(s) 731
TauI GCSGC 2 cut(s) 80, 773
TscAI CASTG 2 cut(s) 214, 478
TseI GCWGC 2 cut(s) 143, 694
TspDTI ATGAA 2 cut(s) 410, 833
TspRI CASTG 2 cut(s) 214, 478
Van91I CCANNNNNTGG 1 cut(s) 762
VpaK11BI GGWCC 4 cut(s) 69, 409, 561, 597
XapI RAATTY 2 cut(s) 289, 321
XspI CTAG 2 cut(s) 81, 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.