Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0012753

Physical Location & Seq

Genomic Coordinates Reverse (-)

652334 .. 654307

Gene Structure 1974 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g01180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGGACGTTTCTCATCGATTCACGATCTGTGCTTTTACCATTTTGTTTATTTTTGAATCCGGATATGGGTTTTACCTTCCGGGTAGTTATCCTCACAAGTACCTTGTTGGGAGCAACTTAGGGGTGAAGGTGAATTCTCTGACTTCCATCGATACCGAGCTGCCCTATAGCTATTACAGTCTCCCCTTTTGTCAGCCCCCAGATGGTGTTAAGGACAGTGCCGAGAATCTCGGTGAGTTCCTTATGGGGGACCGCATTGAGAGCTCTCCGTATCGGTTTAAGATGCACACCAATGAGACTGAGATCTTCTTGTGTGAGACGGGTCCGTTGTCGGCAGATAAGTTCAGTCTCTTGAAGCAGAGGATTGATGAGATGTATCAGGTCAATCTGATTCTTGATAATTTGCCTGCGATCCGGTATACCAAGAAGGAGGGATTTTTGTTGCGGTGGACTGGGTATCCTGTGGGAATTAAGGTTAAGGATGTGTACTATGTGTTTAACCATTTAAAGTTTAAGGTGCTTGTTCATAAATATGAAGAGCCCAACGTAGCACGTGTGATGGGTACTGGCGATGGGTCGGAGGTGATCCCAGCAGTTGCTAAATCAGATTCAGATGAGCCTGGTTATATCATTGTTGGGTTTGAGGTGATACCTTGCAGTTTCATGCACAATGTGGATTCTGTGAAGAAGTTGAAAATGTATGAAAAGTACCCTGCAAAGATACAGTGTGATCCCACCACTGTGGCAATGCCGATTAATGAAAAGCAGTCGGTCGTGTTCACATATGAGGTTGAATTCGAGGAGAGTGACATCAAGTGGCCCTCACGGTGGGATGCTTATTTGAAGATGGAGGGATCGAAAGTCCATTGGTTCTCTATTCTCAATTCTCTGATGGTGATTACTTTCCTTGCTGGTATTGTCCTTGTGATCTTCTTGAGAACTGTTCGGCGGGATCTTACTCGTTATGAAGAGCTTGACAAAGAGGCGCAAGCACAGATGAATGAGGAGCTGTCTGGCTGGAAGCTTGTTGTAGGGGATGTTTTCCGTGCTCCAAGCAATCCTGCCCTGTTGTGTATAATGGTTGGTGATGGTGTTCAGATCCTTGGGATGGCAGTGGTGACCATACTTTTTGCTGCTTTGGGGTTCATGTCGCCTGCTTCTCGTGGAACACTTGTTACAGGTATGCTGTTTTTCTTTATGATCCTTGGTGGTGCAGCTGGTTATGTTGCTGTTCGTCTTTGGAGGACAATTGGCTGTGGAGATCACAAGGGATGGGTTTCGGTCTCATGGAAGGTCGCTTGTTTCTTCCCCGGAATTGCCTTTTTGATTCTGACCACCTTGAATTTCCTGTTGTGGGGTAGTCACAGCACAGGAGCCATTCCCTTTTCTATCTTTGTTATTCTTCTTTTGCTGTGGTTCTGCATTTCTGTTCCCCTTACTCTTGTTGGTGGATACCTTGGGGCCAAGGCACCTCACATAGAGTACCCAGTTCGAACCAATCAAATTCCTAGGGAAATCCCTGCGCAGAAATACCCGTCCTGGCTGTTGGTTCTTGGGGCTGGCACTCTCCCCTTTGGCACCCTATTCATTGAGCTCTTCTTTATCATGTCTAGCATTTGGATGGGCCGTGTCTATTATGTGTTTGGGTTTCTCTTCATCGTATTGATCCTTCTCTTTGTGGTCTGTGCTGAGGTATCTCTGGTTCTAACCTACATGCATCTTTGTGTGGAGGACTGGAAATGGTGGTGGAAGTCATTCTTTGCTTCTGGTTCGGTTGCCATATACATTTTCCTGTACTCCGTAAACTACCTTGTCTTCGATCTCAAGAGCCTGAGCGGACCTGTCTCTGCAACTCTTTACTTGGGGTATTCGCTCCTCATGGTTGTGGCGATCATGCTTGCGACAGGCACAGTCGGGTTGCTCTCTTCATTCTGGTTCGTGCACTACCTGTTCTCTTCGGTGAAGCTGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0008150 GO:0012505 GO:0016020 GO:0016021 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033157 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0050789 GO:0051049 GO:0051050 GO:0051222 GO:0051223 GO:0060341 GO:0065007 GO:0070201 GO:0070861 GO:0070863 GO:0090087 GO:0090316 GO:0097708 GO:0098791 GO:1903827 GO:1903829 GO:1904951 GO:2000008 GO:2000010

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

658

Amino Acids

74.18

Weight (kDa)

6.34

Isoelectric Point (pI)

39.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	55 - 610	4.4e-183	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012753)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35160 AT5G35160 AT5G35160 AT5G35160
malus_domestica	MD02G1012600.v1.1 MD15G1158400.v1.1
prunus_persica	Prupe.7G253600_v2.0.a1
pyrus_communis	pycom02g01180 pycom15g14210
rosa_chinensis	RchiOBHm_Chr2g0086541
rosa_laevigata	RLG00000015765
rosa_multiflora	Rmu_co8340319.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154460
rosa_samantha	Rh2AG018700 Rh2BG018300 Rh2CG019200 Rh2DG020000
rosa_wichuraiana	Rw2G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1524
AccB1I	GGYRCC	2 cut(s)	1468, 1577
AccBSI	CCGCTC	1 cut(s)	1836
AccI	GTMKAC	1 cut(s)	419
AccIII	TCCGGA	1 cut(s)	59
AciI	CCGC	4 cut(s)	253, 445, 949, 1836
AclWI	GGATC	8 cut(s)	406, 580, 725, 862, 960, 1093, 1195, 1660
AcsI	RAATTY	4 cut(s)	133, 794, 1342, 1503
AcvI	CACGTG	1 cut(s)	554
AdeI	CACNNNGTG	1 cut(s)	673
AfaI	GTAC	6 cut(s)	101, 488, 565, 710, 1484, 1796
AfiI	CCNNNNNNNGG	6 cut(s)	66, 203, 247, 828, 1108, 1354
AflIII	ACRYGT	1 cut(s)	553
AgsI	TTSAA	6 cut(s)	56, 355, 694, 794, 844, 1342
AjnI	CCWGG	2 cut(s)	619, 1538
AleI	CACNNNNGTG	1 cut(s)	740
AluBI	AGCT	9 cut(s)	160, 171, 264, 973, 1009, 1024, 1217, 1594, 1966
AluI	AGCT	9 cut(s)	160, 171, 264, 973, 1009, 1024, 1217, 1594, 1966
Alw21I	GWGCWC	4 cut(s)	266, 1051, 1596, 1944
Alw26I	GTCTC	6 cut(s)	185, 290, 311, 353, 1288, 1849
Alw44I	GTGCAC	1 cut(s)	1940
AlwI	GGATC	8 cut(s)	406, 580, 725, 862, 960, 1093, 1195, 1660
Aor13HI	TCCGGA	1 cut(s)	59
AoxI	GGCC	3 cut(s)	818, 1461, 1624
ApaLI	GTGCAC	1 cut(s)	1940
ApeKI	GCWGC	3 cut(s)	160, 1133, 1214
ApoI	RAATTY	4 cut(s)	133, 794, 1342, 1503
AseI	ATTAAT	1 cut(s)	756
Asp700I	GAANNNNTTC	1 cut(s)	1754
AspA2I	CCTAGG	1 cut(s)	1508
AspLEI	GCGC	2 cut(s)	988, 1525
AspS9I	GGNCC	6 cut(s)	250, 323, 819, 1461, 1624, 1838
AsuC2I	CCSGG	2 cut(s)	81, 1311
AsuHPI	GGTGA	9 cut(s)	136, 142, 245, 595, 658, 907, 1097, 1129, 1972
AsuII	TTCGAA	1 cut(s)	1492
AvaII	GGWCC	3 cut(s)	250, 323, 1838
AvrII	CCTAGG	1 cut(s)	1508
BaeGI	GKGCMC	1 cut(s)	1944
BanI	GGYRCC	2 cut(s)	1468, 1577
BanII	GRGCYC	3 cut(s)	266, 543, 1596
BarI	GAAGNNNNNNTAC	2 cut(s)	1653, 1685
BauI	CACGAG	1 cut(s)	1161
BbrPI	CACGTG	1 cut(s)	554
BbsI	GAAGAC	1 cut(s)	1807
Bbv12I	GWGCWC	4 cut(s)	266, 1051, 1596, 1944
BbvCI	CCTCAGC	1 cut(s)	1689
BbvI	GCAGC	3 cut(s)	147, 1120, 1226
BceAI	ACGGC	1 cut(s)	1611
BciT130I	CCWGG	2 cut(s)	621, 1540
BciVI	GTATCC	2 cut(s)	468, 1445
BcnI	CCSGG	2 cut(s)	81, 1311
BcoDI	GTCTC	6 cut(s)	185, 290, 311, 353, 1288, 1849
BfaI	CTAG	2 cut(s)	1509, 1611
BfmI	CTRYAG	1 cut(s)	166
BfuI	GTATCC	2 cut(s)	468, 1445
BglII	AGATCT	1 cut(s)	303
BisI	GCNGC	3 cut(s)	161, 1134, 1215
BlnI	CCTAGG	1 cut(s)	1508
BlsI	GCNGC	3 cut(s)	162, 1135, 1216
Bme1390I	CCNGG	4 cut(s)	81, 621, 1311, 1540
Bme18I	GGWCC	3 cut(s)	250, 323, 1838
BmgT120I	GGNCC	6 cut(s)	250, 323, 819, 1461, 1624, 1838
BmiI	GGNNCC	6 cut(s)	251, 324, 1375, 1462, 1470, 1579
BmrFI	CCNGG	4 cut(s)	81, 621, 1311, 1540
BmrI	ACTGGG	2 cut(s)	462, 1481
BmsI	GCATC	3 cut(s)	273, 823, 1726
BmuI	ACTGGG	2 cut(s)	462, 1481
BpiI	GAAGAC	1 cut(s)	1807
Bpu10I	CCTNAGC	2 cut(s)	1689, 1832
Bpu14I	TTCGAA	1 cut(s)	1492
BpuEI	CTTGAG	2 cut(s)	955, 1808
BpuMI	CCSGG	2 cut(s)	81, 1311
Bsa29I	ATCGAT	2 cut(s)	16, 150
BsaAI	YACGTR	1 cut(s)	554
BsaI	GGTCTC	1 cut(s)	1288
BsaJI	CCNNGG	6 cut(s)	1102, 1204, 1309, 1456, 1464, 1508
BsaWI	WCCGGW	2 cut(s)	59, 414
Bsc4I	CCNNNNNNNGG	6 cut(s)	66, 203, 247, 828, 1108, 1354
Bse1I	ACTGG	4 cut(s)	457, 571, 1487, 1739
Bse3DI	GCAATG	1 cut(s)	753
BseAI	TCCGGA	1 cut(s)	59
BseBI	CCWGG	2 cut(s)	621, 1540
BseCI	ATCGAT	2 cut(s)	16, 150
BseDI	CCNNGG	6 cut(s)	1102, 1204, 1309, 1456, 1464, 1508
BseGI	GGATG	6 cut(s)	487, 838, 1042, 1113, 1277, 1626
BseLI	CCNNNNNNNGG	6 cut(s)	66, 203, 247, 828, 1108, 1354
BseMI	GCAATG	1 cut(s)	753
BseMII	CTCAG	3 cut(s)	291, 1680, 1823
BseNI	ACTGG	4 cut(s)	457, 571, 1487, 1739
BseRI	GAGGAG	3 cut(s)	815, 1019, 1865
BseSI	GKGCMC	1 cut(s)	1944
BseXI	GCAGC	3 cut(s)	147, 1120, 1226
BseYI	CCCAGC	1 cut(s)	589
BsgI	GTGCAG	1 cut(s)	1233
Bsh1285I	CGRYCG	1 cut(s)	774
BshFI	GGCC	3 cut(s)	820, 1463, 1626
BshNI	GGYRCC	2 cut(s)	1468, 1577
BshVI	ATCGAT	2 cut(s)	16, 150
BsiEI	CGRYCG	1 cut(s)	774
BsiHKAI	GWGCWC	4 cut(s)	266, 1051, 1596, 1944
BsiSI	CCGG	4 cut(s)	60, 80, 415, 1311
BslFI	GGGAC	1 cut(s)	263
BslI	CCNNNNNNNGG	6 cut(s)	66, 203, 247, 828, 1108, 1354
BsmAI	GTCTC	6 cut(s)	185, 290, 311, 353, 1288, 1849
BsmBI	CGTCTC	1 cut(s)	311
BsmFI	GGGAC	1 cut(s)	263
BsnI	GGCC	3 cut(s)	820, 1463, 1626
Bso31I	GGTCTC	1 cut(s)	1288
Bsp119I	TTCGAA	1 cut(s)	1492
Bsp1286I	GDGCHC	5 cut(s)	266, 543, 1051, 1596, 1944
Bsp13I	TCCGGA	1 cut(s)	59
BspACI	CCGC	4 cut(s)	253, 445, 949, 1836
BspANI	GGCC	3 cut(s)	820, 1463, 1626
BspCNI	CTCAG	3 cut(s)	292, 1681, 1824
BspDI	ATCGAT	2 cut(s)	16, 150
BspEI	TCCGGA	1 cut(s)	59
BspLI	GGNNCC	6 cut(s)	251, 324, 1375, 1462, 1470, 1579
BspPI	GGATC	8 cut(s)	406, 580, 725, 862, 960, 1093, 1195, 1660
BspQI	GCTCTTC	3 cut(s)	531, 963, 1601
BspT104I	TTCGAA	1 cut(s)	1492
BspT107I	GGYRCC	2 cut(s)	1468, 1577
BspTNI	GGTCTC	1 cut(s)	1288
BsrBI	CCGCTC	1 cut(s)	1836
BsrDI	GCAATG	1 cut(s)	753
BsrI	ACTGG	4 cut(s)	457, 571, 1487, 1739
BssECI	CCNNGG	6 cut(s)	1102, 1204, 1309, 1456, 1464, 1508
BssNAI	GTATAC	1 cut(s)	420
BssSI	CACGAG	1 cut(s)	1161
BssT1I	CCWWGG	5 cut(s)	1102, 1204, 1456, 1464, 1508
Bst1107I	GTATAC	1 cut(s)	420
Bst2BI	CACGAG	1 cut(s)	1161
Bst2UI	CCWGG	2 cut(s)	621, 1540
Bst4CI	ACNGT	7 cut(s)	179, 218, 726, 742, 828, 943, 1912
Bst6I	CTCTTC	6 cut(s)	531, 963, 1601, 1658, 1930, 1960
BstBAI	YACGTR	1 cut(s)	554
BstBI	TTCGAA	1 cut(s)	1492
BstC8I	GCNNGC	5 cut(s)	408, 990, 1155, 1561, 1899
BstDEI	CTNAG	4 cut(s)	118, 300, 1689, 1832
BstEII	GGTNACC	1 cut(s)	1117
BstF5I	GGATG	6 cut(s)	487, 838, 1042, 1113, 1277, 1626
BstHHI	GCGC	2 cut(s)	988, 1525
BstMAI	GTCTC	6 cut(s)	185, 290, 311, 353, 1288, 1849
BstMCI	CGRYCG	1 cut(s)	774
BstMWI	GCNNNNNNNGC	1 cut(s)	261
BstNI	CCWGG	2 cut(s)	621, 1540
BstNSI	RCATGY	1 cut(s)	1717
BstPI	GGTNACC	1 cut(s)	1117
BstSCI	CCNGG	4 cut(s)	79, 619, 1309, 1538
BstSFI	CTRYAG	1 cut(s)	166
BstSLI	GKGCMC	1 cut(s)	1944
BstV1I	GCAGC	3 cut(s)	147, 1120, 1226
BstV2I	GAAGAC	1 cut(s)	1807
BstX2I	RGATCY	3 cut(s)	303, 952, 1098
BstXI	CCANNNNNNTGG	1 cut(s)	742
BstYI	RGATCY	3 cut(s)	303, 952, 1098
BstZ17I	GTATAC	1 cut(s)	420
Bsu15I	ATCGAT	2 cut(s)	16, 150
BsuI	GTATCC	2 cut(s)	468, 1445
BsuRI	GGCC	3 cut(s)	820, 1463, 1626
BsuTUI	ATCGAT	2 cut(s)	16, 150
BtgZI	GCGATG	1 cut(s)	585
BtsCI	GGATG	6 cut(s)	487, 838, 1042, 1113, 1277, 1626
BtsI	GCAGTG	1 cut(s)	1119
BtsIMutI	CAGTG	4 cut(s)	223, 731, 738, 1119
Cac8I	GCNNGC	5 cut(s)	408, 990, 1155, 1561, 1899
CfoI	GCGC	2 cut(s)	988, 1525
Cfr13I	GGNCC	6 cut(s)	250, 323, 819, 1461, 1624, 1838
ClaI	ATCGAT	2 cut(s)	16, 150
Csp6I	GTAC	6 cut(s)	100, 487, 564, 709, 1483, 1795
CviAII	CATG	7 cut(s)	664, 1147, 1287, 1606, 1714, 1879, 1894
CviQI	GTAC	6 cut(s)	100, 487, 564, 709, 1483, 1795
DdeI	CTNAG	4 cut(s)	118, 300, 1689, 1832
DraI	TTTAAA	1 cut(s)	507
DraIII	CACNNNGTG	1 cut(s)	673
Eam1104I	CTCTTC	6 cut(s)	531, 963, 1601, 1658, 1930, 1960
EarI	CTCTTC	6 cut(s)	531, 963, 1601, 1658, 1930, 1960
Ecl136II	GAGCTC	2 cut(s)	264, 1594
Eco130I	CCWWGG	5 cut(s)	1102, 1204, 1456, 1464, 1508
Eco24I	GRGCYC	3 cut(s)	266, 543, 1596
Eco31I	GGTCTC	1 cut(s)	1288
Eco47I	GGWCC	3 cut(s)	250, 323, 1838
Eco53kI	GAGCTC	2 cut(s)	264, 1594
Eco72I	CACGTG	1 cut(s)	554
Eco91I	GGTNACC	1 cut(s)	1117
EcoICRI	GAGCTC	2 cut(s)	264, 1594
EcoO65I	GGTNACC	1 cut(s)	1117
EcoRI	GAATTC	2 cut(s)	133, 794
EcoRII	CCWGG	2 cut(s)	619, 1538
EcoT14I	CCWWGG	5 cut(s)	1102, 1204, 1456, 1464, 1508
EcoT22I	ATGCAT	1 cut(s)	1719
EcoT38I	GRGCYC	3 cut(s)	266, 543, 1596
ErhI	CCWWGG	5 cut(s)	1102, 1204, 1456, 1464, 1508
Esp3I	CGTCTC	1 cut(s)	311
FaeI	CATG	7 cut(s)	667, 1150, 1290, 1609, 1717, 1882, 1897
FalI	AAGNNNNNCTT	2 cut(s)	1742, 1774
FaqI	GGGAC	1 cut(s)	263
FatI	CATG	7 cut(s)	663, 1146, 1286, 1605, 1713, 1878, 1893
FauI	CCCGC	1 cut(s)	942
FauNDI	CATATG	1 cut(s)	784
FblI	GTMKAC	1 cut(s)	419
Fnu4HI	GCNGC	3 cut(s)	161, 1134, 1215
FokI	GGATG	6 cut(s)	494, 845, 1049, 1120, 1284, 1633
FriOI	GRGCYC	3 cut(s)	266, 543, 1596
Fsp4HI	GCNGC	3 cut(s)	161, 1134, 1215
FspBI	CTAG	2 cut(s)	1509, 1611
FspI	TGCGCA	1 cut(s)	1524
GlaI	GCGC	2 cut(s)	987, 1524
GluI	GCNGC	3 cut(s)	161, 1134, 1215
GsaI	CCCAGC	1 cut(s)	593
HaeIII	GGCC	3 cut(s)	820, 1463, 1626
HapII	CCGG	4 cut(s)	60, 80, 415, 1311
HhaI	GCGC	2 cut(s)	988, 1525
Hin1II	CATG	7 cut(s)	667, 1150, 1290, 1609, 1717, 1882, 1897
Hin6I	GCGC	2 cut(s)	986, 1523
HinP1I	GCGC	2 cut(s)	986, 1523
HindIII	AAGCTT	1 cut(s)	1022
HinfI	GANTC	7 cut(s)	18, 56, 226, 391, 608, 677, 1327
HpaII	CCGG	4 cut(s)	60, 80, 415, 1311
HphI	GGTGA	9 cut(s)	136, 142, 245, 595, 658, 907, 1097, 1129, 1972
Hpy166II	GTNNAC	6 cut(s)	420, 450, 487, 780, 1804, 1942
Hpy188I	TCNGA	8 cut(s)	141, 390, 580, 607, 613, 891, 1098, 1332
Hpy188III	TCNNGA	6 cut(s)	22, 60, 352, 395, 934, 1825
Hpy8I	GTNNAC	6 cut(s)	420, 450, 487, 780, 1804, 1942
HpyAV	CCTTC	5 cut(s)	86, 121, 421, 1285, 1679
HpyCH4III	ACNGT	7 cut(s)	179, 218, 726, 742, 828, 943, 1912
HpyCH4IV	ACGT	3 cut(s)	6, 546, 553
HpyCH4V	TGCA	9 cut(s)	286, 657, 667, 716, 1214, 1422, 1717, 1850, 1942
HpyF10VI	GCNNNNNNNGC	1 cut(s)	261
HpyF3I	CTNAG	4 cut(s)	118, 300, 1689, 1832
HpySE526I	ACGT	3 cut(s)	6, 546, 553
Hsp92II	CATG	7 cut(s)	667, 1150, 1290, 1609, 1717, 1882, 1897
HspAI	GCGC	2 cut(s)	986, 1523
Kpn2I	TCCGGA	1 cut(s)	59
LguI	GCTCTTC	3 cut(s)	531, 963, 1601
LmnI	GCTCC	5 cut(s)	111, 1006, 1054, 1373, 1878
Lsp1109I	GCAGC	3 cut(s)	147, 1120, 1226
LweI	GCATC	3 cut(s)	273, 823, 1726
MaeI	CTAG	2 cut(s)	1509, 1611
MaeII	ACGT	3 cut(s)	6, 546, 553
MaeIII	GTNAC	4 cut(s)	806, 1117, 1174, 1361
MbiI	CCGCTC	1 cut(s)	1836
MfeI	CAATTG	1 cut(s)	1248
MflI	RGATCY	3 cut(s)	303, 952, 1098
MhlI	GDGCHC	5 cut(s)	266, 543, 1051, 1596, 1944
MluCI	AATT	9 cut(s)	133, 400, 468, 794, 883, 1248, 1314, 1342, 1503
MmeI	TCCRAC	1 cut(s)	558
Mph1103I	ATGCAT	1 cut(s)	1719
MroI	TCCGGA	1 cut(s)	59
MroXI	GAANNNNTTC	1 cut(s)	1754
MseI	TTAA	8 cut(s)	210, 279, 471, 477, 498, 506, 513, 756
MslI	CAYNNNNRTG	6 cut(s)	291, 531, 740, 1619, 1722, 1883
MspA1I	CMGCKG	1 cut(s)	1217
MspI	CCGG	4 cut(s)	60, 80, 415, 1311
MspR9I	CCNGG	4 cut(s)	81, 621, 1311, 1540
MunI	CAATTG	1 cut(s)	1248
MvaI	CCWGG	2 cut(s)	621, 1540
MwoI	GCNNNNNNNGC	1 cut(s)	261
NciI	CCSGG	2 cut(s)	81, 1311
NdeI	CATATG	1 cut(s)	784
NlaIII	CATG	7 cut(s)	667, 1150, 1290, 1609, 1717, 1882, 1897
NlaIV	GGNNCC	6 cut(s)	251, 324, 1375, 1462, 1470, 1579
NmeAIII	GCCGAG	1 cut(s)	247
NmuCI	GTSAC	3 cut(s)	806, 1117, 1361
NsbI	TGCGCA	1 cut(s)	1524
NsiI	ATGCAT	1 cut(s)	1719
NspI	RCATGY	1 cut(s)	1717
NspV	TTCGAA	1 cut(s)	1492
OliI	CACNNNNGTG	1 cut(s)	740
PciSI	GCTCTTC	3 cut(s)	531, 963, 1601
PdmI	GAANNNNTTC	1 cut(s)	1754
PfeI	GAWTC	7 cut(s)	18, 56, 226, 391, 608, 677, 1327
PkrI	GCNGC	3 cut(s)	162, 1135, 1216
PmaCI	CACGTG	1 cut(s)	554
PmlI	CACGTG	1 cut(s)	554
Ppu21I	YACGTR	1 cut(s)	554
PshBI	ATTAAT	1 cut(s)	756
Psp124BI	GAGCTC	2 cut(s)	266, 1596
Psp6I	CCWGG	2 cut(s)	619, 1538
PspCI	CACGTG	1 cut(s)	554
PspEI	GGTNACC	1 cut(s)	1117
PspFI	CCCAGC	1 cut(s)	589
PspGI	CCWGG	2 cut(s)	619, 1538
PspN4I	GGNNCC	6 cut(s)	251, 324, 1375, 1462, 1470, 1579
PspPI	GGNCC	6 cut(s)	250, 323, 819, 1461, 1624, 1838
PsrI	GAACNNNNNNTAC	4 cut(s)	1159, 1191, 1686, 1718
PsuI	RGATCY	3 cut(s)	303, 952, 1098
PvuII	CAGCTG	1 cut(s)	1217
RsaI	GTAC	6 cut(s)	101, 488, 565, 710, 1484, 1796
RsaNI	GTAC	6 cut(s)	100, 487, 564, 709, 1483, 1795
RseI	CAYNNNNRTG	6 cut(s)	291, 531, 740, 1619, 1722, 1883
SacI	GAGCTC	2 cut(s)	266, 1596
SapI	GCTCTTC	3 cut(s)	531, 963, 1601
SaqAI	TTAA	8 cut(s)	210, 279, 471, 477, 498, 506, 513, 756
SatI	GCNGC	3 cut(s)	161, 1134, 1215
Sau96I	GGNCC	6 cut(s)	250, 323, 819, 1461, 1624, 1838
ScrFI	CCNGG	4 cut(s)	81, 621, 1311, 1540
SduI	GDGCHC	5 cut(s)	266, 543, 1051, 1596, 1944
SfaNI	GCATC	3 cut(s)	273, 823, 1726
SfcI	CTRYAG	1 cut(s)	166
SfuI	TTCGAA	1 cut(s)	1492
SinI	GGWCC	3 cut(s)	250, 323, 1838
SmiMI	CAYNNNNRTG	6 cut(s)	291, 531, 740, 1619, 1722, 1883
SmlI	CTYRAG	2 cut(s)	934, 1823
SmoI	CTYRAG	2 cut(s)	934, 1823
Sse9I	AATT	9 cut(s)	133, 400, 468, 794, 883, 1248, 1314, 1342, 1503
SsiI	CCGC	4 cut(s)	253, 445, 949, 1836
SspMI	CTAG	2 cut(s)	1509, 1611
SstI	GAGCTC	2 cut(s)	266, 1596
StyD4I	CCNGG	4 cut(s)	79, 619, 1309, 1538
StyI	CCWWGG	5 cut(s)	1102, 1204, 1456, 1464, 1508
TaaI	ACNGT	7 cut(s)	179, 218, 726, 742, 828, 943, 1912
TaiI	ACGT	3 cut(s)	9, 549, 556
TaqI	TCGA	6 cut(s)	16, 150, 798, 857, 1492, 1818
TaqII	GACCGA	2 cut(s)	760, 1270
TasI	AATT	9 cut(s)	133, 400, 468, 794, 883, 1248, 1314, 1342, 1503
TatI	WGTACW	2 cut(s)	486, 1794
TfiI	GAWTC	7 cut(s)	18, 56, 226, 391, 608, 677, 1327
Tru1I	TTAA	8 cut(s)	210, 279, 471, 477, 498, 506, 513, 756
Tru9I	TTAA	8 cut(s)	210, 279, 471, 477, 498, 506, 513, 756
TscAI	CASTG	4 cut(s)	223, 731, 745, 1119
TseFI	GTSAC	3 cut(s)	806, 1117, 1361
TseI	GCWGC	3 cut(s)	160, 1133, 1214
Tsp45I	GTSAC	3 cut(s)	806, 1117, 1361
TspGWI	ACGGA	4 cut(s)	258, 315, 1034, 1789
TspRI	CASTG	4 cut(s)	223, 731, 745, 1119
VneI	GTGCAC	1 cut(s)	1940
VpaK11BI	GGWCC	3 cut(s)	250, 323, 1838
VspI	ATTAAT	1 cut(s)	756
XapI	RAATTY	4 cut(s)	133, 794, 1342, 1503
XceI	RCATGY	1 cut(s)	1717
XmaJI	CCTAGG	1 cut(s)	1508
XmiI	GTMKAC	1 cut(s)	419
XmnI	GAANNNNTTC	1 cut(s)	1754
XspI	CTAG	2 cut(s)	1509, 1611
Zsp2I	ATGCAT	1 cut(s)	1719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.