pycom02g02270

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0024614
Physical Location & Seq
Forward (+)
1393693 .. 1396179
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02270.4

Sequence Viewer

Length: 1140 bp
ATGTCCTCGCTTAGCAGAGAGCTCGTTTTTCTCATACTGCAGTTTCTGGATGAAGAGAAATTCAAAGACAGTGTTCACAAGTTGGAGCAAGAGTCAGGGTTTTTCTTTAACATGAGGTACTTTGAGGACATGGTGACAAATGGAGAGTGGGAGGAGGTGGAAAAGTATTTGTCTGGTTTCACGAAGGTTGATGATAATCGATACTCTATGAAGATTTTCTTCGAGATTCGCAAGCAGAAGTACCTTGAAGCTTTAGACAAGCGGGATCGATCAAAAGCTGTGGACATTTTAGTTAAGGACTTGAAAGTTTTTGCATCATTTAATGAGGAACTTTTTAAGGAAATTACACAGTTGTTGACTCTGGAAAACTTTAGGGATAATGAACAGTTATCTAAATATGGTGATACTAAGTCTGCAAGGAGTATAATGCTTGCTGAACTGAAAAAGTTAATAGAGGCTAACCCATTGTTTCGTGATAAACTTCAATTTCCTACCTTGAAGAACTCGAGACTGCGTACTCTTATTAATCAGAGTTTAAACTGGCAGCATCAGCTTTGTAAGAACCCAAGGTCCAATCCAGACATAAAGACCCTGTTTGTAGACCACAGTTGTGGACAACCAAATGGTACCAGGGCTCCATCTCCTGTCACTAATCACTTAATGGGGGCAGTCCCTAAGACAGGGGGTTTCCCTCCATTGGGTGCTCATGGGCCCTTTCAGCCCACACCAGCTGCTCTTCCAACGTCTCTTGCTGGATGGATGGCAAATCCATCACCTGTGCCTCATCCCTCAGCATCTGCTGGGCCTCTTGGGTTGGCCGCAGCTAACACTGCGGCCATCTTGAAGCGCCCTAGAACTCCTCCTACCGGTAACCCGACTATGGACTATCAAACAGCAGATTCTGAGTTCGTTCTGAAGAGATCAAGACCTTTTGGGATACCGGATGAAGCCAATAATCTGCCAGTAAATATGCTGCCTGTTGCATATCCTAATCAGAGCCATGGTCAGAGTTCCTACTCTTCTGATGACTTGCCCAGGTCTGTTGTTATGACTTTAAGTCCGGGTTCAGCTGTCAAGAGTATGGATTTCCACCCGGTGCAACAAATTTTACTTCTTGGTAATTGGAACAAACACGGGTGA

Protein Analysis

380

Amino Acids

42.58

Weight (kDa)

8.64

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024614)

Species Orthologous Gene IDs
pyrus_communis pycom02g02270 pycom12419g00030 pycom15g15100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 626
AccI GTMKAC 1 cut(s) 600
AciI CCGC 3 cut(s) 262, 819, 833
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 2 cut(s) 816, 834
AcsI RAATTY 2 cut(s) 59, 1104
AcuI CTGAAG 1 cut(s) 935
AdeI CACNNNGTG 1 cut(s) 1096
AfaI GTAC 4 cut(s) 119, 242, 517, 628
AfiI CCNNNNNNNGG 5 cut(s) 680, 697, 698, 866, 880
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 6 cut(s) 64, 248, 304, 485, 499, 844
AhdI GACNNNNNGTC 1 cut(s) 1056
AjnI CCWGG 2 cut(s) 629, 1034
AleI CACNNNNGTG 1 cut(s) 609
AloI GAACNNNNNNTCC 2 cut(s) 554, 586
AluBI AGCT 7 cut(s) 22, 251, 278, 553, 731, 824, 1070
AluI AGCT 7 cut(s) 22, 251, 278, 553, 731, 824, 1070
Alw21I GWGCWC 2 cut(s) 24, 706
Alw26I GTCTC 2 cut(s) 502, 750
AlwI GGATC 1 cut(s) 273
AlwNI CAGNNNCTG 3 cut(s) 46, 797, 902
Ama87I CYCGRG 1 cut(s) 505
AoxI GGCC 4 cut(s) 710, 803, 816, 834
ApaI GGGCCC 1 cut(s) 714
ApeKI GCWGC 4 cut(s) 544, 731, 821, 973
ApoI RAATTY 2 cut(s) 59, 1104
AseI ATTAAT 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 866
Asp700I GAANNNNTTC 1 cut(s) 215
Asp718I GGTACC 1 cut(s) 626
AspLEI GCGC 1 cut(s) 849
AspS9I GGNCC 4 cut(s) 570, 710, 711, 803
AsuC2I CCSGG 2 cut(s) 1062, 1094
AsuHPI GGTGA 3 cut(s) 145, 413, 765
AvaI CYCGRG 1 cut(s) 505
AvaII GGWCC 1 cut(s) 570
BaeGI GKGCMC 1 cut(s) 714
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 3 cut(s) 24, 637, 714
Bbv12I GWGCWC 2 cut(s) 24, 706
BbvCI CCTCAGC 1 cut(s) 790
BbvI GCAGC 4 cut(s) 556, 718, 833, 960
BccI CCATC 5 cut(s) 646, 750, 754, 778, 845
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 2 cut(s) 631, 1036
BciVI GTATCC 1 cut(s) 930
BcnI CCSGG 2 cut(s) 1062, 1094
BcoDI GTCTC 2 cut(s) 502, 750
BfaI CTAG 1 cut(s) 852
BfmI CTRYAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 850
BfuI GTATCC 1 cut(s) 930
BisI GCNGC 6 cut(s) 545, 732, 819, 822, 834, 974
BlpI GCTNAGC 1 cut(s) 11
BlsI GCNGC 6 cut(s) 546, 733, 820, 823, 835, 975
Bme1390I CCNGG 4 cut(s) 631, 1036, 1062, 1094
Bme18I GGWCC 1 cut(s) 570
BmeRI GACNNNNNGTC 1 cut(s) 1056
BmeT110I CYCGRG 1 cut(s) 505
BmgT120I GGNCC 4 cut(s) 570, 710, 711, 803
BmiI GGNNCC 3 cut(s) 628, 636, 712
BmrFI CCNGG 4 cut(s) 631, 1036, 1062, 1094
BmsI GCATC 3 cut(s) 323, 556, 803
Bpu10I CCTNAGC 1 cut(s) 790
Bpu1102I GCTNAGC 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 1062, 1094
Bsa29I ATCGAT 2 cut(s) 199, 268
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 566, 630, 1000, 1034
BsaWI WCCGGW 2 cut(s) 866, 940
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 619, 649
Bsc4I CCNNNNNNNGG 5 cut(s) 680, 697, 698, 866, 880
Bse118I RCCGGY 1 cut(s) 866
Bse1I ACTGG 2 cut(s) 545, 962
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 631, 1036
BseCI ATCGAT 2 cut(s) 199, 268
BseDI CCNNGG 4 cut(s) 566, 630, 1000, 1034
BseGI GGATG 5 cut(s) 55, 761, 765, 784, 949
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 5 cut(s) 680, 697, 698, 866, 880
BseMII CTCAG 2 cut(s) 804, 894
BseNI ACTGG 2 cut(s) 545, 962
BseRI GAGGAG 2 cut(s) 167, 849
BseSI GKGCMC 1 cut(s) 714
BseXI GCAGC 4 cut(s) 556, 718, 833, 960
BseYI CCCAGC 1 cut(s) 800
BshFI GGCC 4 cut(s) 712, 805, 818, 836
BshNI GGYRCC 1 cut(s) 626
BshTI ACCGGT 1 cut(s) 866
BshVI ATCGAT 2 cut(s) 199, 268
BsiHKAI GWGCWC 2 cut(s) 24, 706
BsiHKCI CYCGRG 1 cut(s) 505
BsiSI CCGG 4 cut(s) 867, 941, 1061, 1094
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 5 cut(s) 680, 697, 698, 866, 880
BsmAI GTCTC 2 cut(s) 502, 750
BsmBI CGTCTC 1 cut(s) 750
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 4 cut(s) 712, 805, 818, 836
BsoBI CYCGRG 1 cut(s) 505
Bsp120I GGGCCC 1 cut(s) 710
Bsp1286I GDGCHC 4 cut(s) 24, 637, 706, 714
Bsp143I GATC 3 cut(s) 265, 269, 920
Bsp1720I GCTNAGC 1 cut(s) 11
Bsp19I CCATGG 1 cut(s) 1000
BspACI CCGC 3 cut(s) 262, 819, 833
BspANI GGCC 4 cut(s) 712, 805, 818, 836
BspCNI CTCAG 2 cut(s) 803, 895
BspDI ATCGAT 2 cut(s) 199, 268
BspLI GGNNCC 3 cut(s) 628, 636, 712
BspMAI CTGCAG 1 cut(s) 42
BspPI GGATC 1 cut(s) 273
BspQI GCTCTTC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 626
BsrFI RCCGGY 1 cut(s) 866
BsrI ACTGG 2 cut(s) 545, 962
BssAI RCCGGY 1 cut(s) 866
BssECI CCNNGG 4 cut(s) 566, 630, 1000, 1034
BssMI GATC 3 cut(s) 265, 269, 920
BssT1I CCWWGG 2 cut(s) 566, 1000
Bst2UI CCWGG 2 cut(s) 631, 1036
Bst4CI ACNGT 4 cut(s) 71, 351, 387, 608
Bst6I CTCTTC 4 cut(s) 48, 741, 911, 1024
BstC8I GCNNGC 2 cut(s) 233, 432
BstDEI CTNAG 5 cut(s) 11, 408, 675, 790, 903
BstDSI CCRYGG 1 cut(s) 1000
BstEII GGTNACC 1 cut(s) 869
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 5 cut(s) 55, 761, 765, 784, 949
BstH2I RGCGCY 1 cut(s) 850
BstHHI GCGC 1 cut(s) 849
BstKTI GATC 3 cut(s) 268, 272, 923
BstMAI GTCTC 2 cut(s) 502, 750
BstMBI GATC 3 cut(s) 265, 269, 920
BstMWI GCNNNNNNNGC 3 cut(s) 550, 718, 830
BstNI CCWGG 2 cut(s) 631, 1036
BstPI GGTNACC 1 cut(s) 869
BstSCI CCNGG 4 cut(s) 629, 1034, 1060, 1092
BstSFI CTRYAG 1 cut(s) 38
BstSLI GKGCMC 1 cut(s) 714
BstV1I GCAGC 4 cut(s) 556, 718, 833, 960
BstXI CCANNNNNNTGG 1 cut(s) 611
Bsu15I ATCGAT 2 cut(s) 199, 268
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 4 cut(s) 712, 805, 818, 836
BsuTUI ATCGAT 2 cut(s) 199, 268
BtgI CCRYGG 1 cut(s) 1000
BtsCI GGATG 5 cut(s) 55, 761, 765, 784, 949
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 76, 828
Cac8I GCNNGC 2 cut(s) 233, 432
CaiI CAGNNNCTG 3 cut(s) 46, 797, 902
CfoI GCGC 1 cut(s) 849
Cfr10I RCCGGY 1 cut(s) 866
Cfr13I GGNCC 4 cut(s) 570, 710, 711, 803
ClaI ATCGAT 2 cut(s) 199, 268
Csp6I GTAC 4 cut(s) 118, 241, 516, 627
CspAI ACCGGT 1 cut(s) 866
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviAII CATG 4 cut(s) 112, 130, 707, 1001
CviQI GTAC 4 cut(s) 118, 241, 516, 627
DdeI CTNAG 5 cut(s) 11, 408, 675, 790, 903
DpnI GATC 3 cut(s) 267, 271, 922
DpnII GATC 3 cut(s) 265, 269, 920
DraI TTTAAA 1 cut(s) 537
DraIII CACNNNGTG 1 cut(s) 1096
DriI GACNNNNNGTC 1 cut(s) 1056
EaeI YGGCCR 2 cut(s) 816, 834
Eam1104I CTCTTC 4 cut(s) 48, 741, 911, 1024
Eam1105I GACNNNNNGTC 1 cut(s) 1056
EarI CTCTTC 4 cut(s) 48, 741, 911, 1024
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 566, 1000
Eco24I GRGCYC 3 cut(s) 24, 637, 714
Eco47I GGWCC 1 cut(s) 570
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 935
Eco88I CYCGRG 1 cut(s) 505
Eco91I GGTNACC 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 711
EcoO65I GGTNACC 1 cut(s) 869
EcoRII CCWGG 2 cut(s) 629, 1034
EcoT14I CCWWGG 2 cut(s) 566, 1000
EcoT38I GRGCYC 3 cut(s) 24, 637, 714
ErhI CCWWGG 2 cut(s) 566, 1000
Esp3I CGTCTC 1 cut(s) 750
FaeI CATG 4 cut(s) 115, 133, 710, 1004
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 1 cut(s) 656
FatI CATG 4 cut(s) 111, 129, 706, 1000
FauI CCCGC 1 cut(s) 255
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 6 cut(s) 545, 732, 819, 822, 834, 974
FokI GGATG 5 cut(s) 62, 768, 771, 772, 956
FriOI GRGCYC 3 cut(s) 24, 637, 714
Fsp4HI GCNGC 6 cut(s) 545, 732, 819, 822, 834, 974
FspBI CTAG 1 cut(s) 852
GlaI GCGC 1 cut(s) 848
GluI GCNGC 6 cut(s) 545, 732, 819, 822, 834, 974
GsaI CCCAGC 1 cut(s) 804
HaeII RGCGCY 1 cut(s) 850
HaeIII GGCC 4 cut(s) 712, 805, 818, 836
HapII CCGG 4 cut(s) 867, 941, 1061, 1094
HhaI GCGC 1 cut(s) 849
Hin1II CATG 4 cut(s) 115, 133, 710, 1004
Hin6I GCGC 1 cut(s) 847
HinP1I GCGC 1 cut(s) 847
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 4 cut(s) 92, 226, 358, 899
HpaII CCGG 4 cut(s) 867, 941, 1061, 1094
HphI GGTGA 3 cut(s) 145, 413, 765
Hpy166II GTNNAC 5 cut(s) 76, 283, 357, 601, 614
Hpy188I TCNGA 6 cut(s) 531, 904, 915, 996, 1008, 1024
Hpy8I GTNNAC 5 cut(s) 76, 283, 357, 601, 614
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 4 cut(s) 71, 351, 387, 608
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 5 cut(s) 40, 314, 416, 983, 1099
HpyF10VI GCNNNNNNNGC 3 cut(s) 550, 718, 830
HpyF3I CTNAG 5 cut(s) 11, 408, 675, 790, 903
HpySE526I ACGT 1 cut(s) 743
Hsp92II CATG 4 cut(s) 115, 133, 710, 1004
HspAI GCGC 1 cut(s) 847
KpnI GGTACC 1 cut(s) 630
Kzo9I GATC 3 cut(s) 265, 269, 920
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 2 cut(s) 85, 640
Lsp1109I GCAGC 4 cut(s) 556, 718, 833, 960
LweI GCATC 3 cut(s) 323, 556, 803
MaeI CTAG 1 cut(s) 852
MaeII ACGT 1 cut(s) 743
MaeIII GTNAC 3 cut(s) 133, 646, 869
MalI GATC 3 cut(s) 267, 271, 922
MboI GATC 3 cut(s) 265, 269, 920
MboII GAAGA 7 cut(s) 65, 211, 223, 511, 728, 928, 1011
MhlI GDGCHC 4 cut(s) 24, 637, 706, 714
MluCI AATT 5 cut(s) 59, 342, 485, 1104, 1120
MlyI GAGTC 2 cut(s) 101, 352
MmeI TCCRAC 2 cut(s) 63, 764
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 9 cut(s) 108, 294, 321, 336, 449, 525, 536, 659, 1055
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 2 cut(s) 731, 1070
MspI CCGG 4 cut(s) 867, 941, 1061, 1094
MspR9I CCNGG 4 cut(s) 631, 1036, 1062, 1094
MssI GTTTAAAC 1 cut(s) 537
MvaI CCWGG 2 cut(s) 631, 1036
MwoI GCNNNNNNNGC 3 cut(s) 550, 718, 830
NciI CCSGG 2 cut(s) 1062, 1094
NcoI CCATGG 1 cut(s) 1000
NdeII GATC 3 cut(s) 265, 269, 920
NlaIII CATG 4 cut(s) 115, 133, 710, 1004
NlaIV GGNNCC 3 cut(s) 628, 636, 712
NmuCI GTSAC 2 cut(s) 133, 646
OliI CACNNNNGTG 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 226, 899
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 6 cut(s) 546, 733, 820, 823, 835, 975
PleI GAGTC 2 cut(s) 100, 352
PmeI GTTTAAAC 1 cut(s) 537
PpsI GAGTC 2 cut(s) 100, 352
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 629, 1034
PspEI GGTNACC 1 cut(s) 869
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 2 cut(s) 629, 1034
PspN4I GGNNCC 3 cut(s) 628, 636, 712
PspOMI GGGCCC 1 cut(s) 710
PspPI GGNCC 4 cut(s) 570, 710, 711, 803
PsrI GAACNNNNNNTAC 2 cut(s) 847, 879
PstI CTGCAG 1 cut(s) 42
PstNI CAGNNNCTG 3 cut(s) 46, 797, 902
PvuII CAGCTG 2 cut(s) 731, 1070
RsaI GTAC 4 cut(s) 119, 242, 517, 628
RsaNI GTAC 4 cut(s) 118, 241, 516, 627
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 9 cut(s) 108, 294, 321, 336, 449, 525, 536, 659, 1055
SatI GCNGC 6 cut(s) 545, 732, 819, 822, 834, 974
Sau3AI GATC 3 cut(s) 265, 269, 920
Sau96I GGNCC 4 cut(s) 570, 710, 711, 803
SchI GAGTC 2 cut(s) 101, 352
ScrFI CCNGG 4 cut(s) 631, 1036, 1062, 1094
SduI GDGCHC 4 cut(s) 24, 637, 706, 714
SfaNI GCATC 3 cut(s) 323, 556, 803
SfcI CTRYAG 1 cut(s) 38
Sfr274I CTCGAG 1 cut(s) 505
SinI GGWCC 1 cut(s) 570
SlaI CTCGAG 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 5 cut(s) 59, 342, 485, 1104, 1120
SsiI CCGC 3 cut(s) 262, 819, 833
SspMI CTAG 1 cut(s) 852
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 4 cut(s) 629, 1034, 1060, 1092
StyI CCWWGG 2 cut(s) 566, 1000
TaaI ACNGT 4 cut(s) 71, 351, 387, 608
TaiI ACGT 1 cut(s) 746
TaqI TCGA 4 cut(s) 199, 222, 268, 506
TasI AATT 5 cut(s) 59, 342, 485, 1104, 1120
TauI GCSGC 2 cut(s) 821, 836
TfiI GAWTC 2 cut(s) 226, 899
Tru1I TTAA 9 cut(s) 108, 294, 321, 336, 449, 525, 536, 659, 1055
Tru9I TTAA 9 cut(s) 108, 294, 321, 336, 449, 525, 536, 659, 1055
TscAI CASTG 2 cut(s) 76, 835
TseFI GTSAC 2 cut(s) 133, 646
TseI GCWGC 4 cut(s) 544, 731, 821, 973
Tsp45I GTSAC 2 cut(s) 133, 646
TspDTI ATGAA 4 cut(s) 66, 224, 396, 960
TspRI CASTG 2 cut(s) 76, 835
VpaK11BI GGWCC 1 cut(s) 570
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 2 cut(s) 59, 1104
XhoI CTCGAG 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 600
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.