pycom02g03160

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022412
Physical Location & Seq
Reverse (-)
2054648 .. 2055334
687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03160.1

Sequence Viewer

Length: 687 bp
ATGTACCTCTTTCTTTCCCCTCTTGTTGTCATTATCGCTGCCCAGATTTCAACATTTTCTCTCAAGGATCTTTATGATCTTTGGGAGCATCTCCAGTACAACCTGATTTCAGTGATTACCTGCTCGACTCTCCTTGTTTTCCTATCCACCGTGTACTTTGTCACCCGCCCTCGCCCGGTGTACCTTGTTAACTTTTCGTGCTACAAGCCAGAAGAATCTCGAAAATGCACAAAAAAGATTTTCATGGAACAGTCTCAGATGACTGGTACATTTACAGAGGACAATCTTCAGTTCCAGCGGAGGATCCTTGAGAGATCCGGCCTTGGTGACTCGACTTACCTTCCGGAGGCAGTTCTCAACATTCCTCCAAATCCGTCAATGAAAGAAGCTAGAAAAGAAGCCGAGGCTGTGATGTTTGGTGCCATTGATGAGCTTTTCGCTAAGACAGCTGTTAAACCTAAGGACATTGGAATCTTGATTGTGAACTGCAGCTTGTTCAATCCAACCCCGTCTCTTTCTGCCATGGTTATCAATCATTACCAGCTTCGAGGGAACATAGTCAGCTATAATCTTGGTGGGATGGGATGCAGTGCAGGTCTGATCGATCGATCGATCTTGCTAAAAATCTTCTTCAGGTGCATCCCAACTCTTATGCACTGGTTATCAGCATGGAGAACATCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

229

Amino Acids

25.87

Weight (kDa)

8.71

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 52 - 202 9.9e-69 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022412)

Species Orthologous Gene IDs
malus_domestica MD00G1026700.v1.1
pyrus_communis pycom02g03160 pycom15g10690
rosa_multiflora Rmu_co8061556.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 128, 584
AccB1I GGYRCC 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 2 cut(s) 166, 298
AclWI GGATC 4 cut(s) 75, 298, 309, 311
AcuI CTGAAG 2 cut(s) 272, 616
AfaI GTAC 5 cut(s) 5, 98, 155, 182, 268
AfiI CCNNNNNNNGG 2 cut(s) 175, 346
AgsI TTSAA 2 cut(s) 51, 499
AluBI AGCT 6 cut(s) 389, 433, 449, 492, 544, 564
AluI AGCT 6 cut(s) 389, 433, 449, 492, 544, 564
Alw26I GTCTC 2 cut(s) 258, 516
AlwI GGATC 4 cut(s) 75, 298, 309, 311
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 38, 489
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 3 cut(s) 154, 338, 672
AxyI CCTNAGG 1 cut(s) 459
BamHI GGATCC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 25, 501
BccI CCATC 1 cut(s) 574
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 2 cut(s) 258, 516
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 487
BfuAI ACCTGC 2 cut(s) 128, 584
BisI GCNGC 2 cut(s) 39, 490
BlsI GCNGC 2 cut(s) 40, 491
Bme1390I CCNGG 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 305, 421
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 3 cut(s) 97, 575, 648
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 2 cut(s) 47, 329
BpuMI CCSGG 1 cut(s) 176
Bsa29I ATCGAT 3 cut(s) 603, 607, 611
BsaJI CCNNGG 3 cut(s) 322, 402, 522
BsaWI WCCGGW 1 cut(s) 343
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 346
Bse1I ACTGG 3 cut(s) 94, 268, 662
Bse21I CCTNAGG 1 cut(s) 459
BseAI TCCGGA 1 cut(s) 343
BseCI ATCGAT 3 cut(s) 603, 607, 611
BseDI CCNNGG 3 cut(s) 322, 402, 522
BseGI GGATG 3 cut(s) 585, 590, 639
BseLI CCNNNNNNNGG 2 cut(s) 175, 346
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 3 cut(s) 94, 268, 662
BseXI GCAGC 2 cut(s) 25, 501
BsgI GTGCAG 1 cut(s) 612
Bsh1285I CGRYCG 2 cut(s) 607, 611
BshFI GGCC 1 cut(s) 321
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 3 cut(s) 603, 607, 611
BsiEI CGRYCG 2 cut(s) 607, 611
BsiSI CCGG 3 cut(s) 176, 318, 344
BslI CCNNNNNNNGG 2 cut(s) 175, 346
BsmAI GTCTC 2 cut(s) 258, 516
BsmBI CGTCTC 1 cut(s) 516
BsnI GGCC 1 cut(s) 321
Bsp13I TCCGGA 1 cut(s) 343
Bsp143I GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 2 cut(s) 166, 298
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 1 cut(s) 268
BspDI ATCGAT 3 cut(s) 603, 607, 611
BspEI TCCGGA 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 305, 421
BspMAI CTGCAG 1 cut(s) 491
BspMI ACCTGC 2 cut(s) 128, 584
BspPI GGATC 4 cut(s) 75, 298, 309, 311
BspT107I GGYRCC 1 cut(s) 419
BsrI ACTGG 3 cut(s) 94, 268, 662
BssECI CCNNGG 3 cut(s) 322, 402, 522
BssMI GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
BssT1I CCWWGG 2 cut(s) 322, 522
Bst4CI ACNGT 2 cut(s) 151, 252
BstDEI CTNAG 3 cut(s) 255, 441, 459
BstDSI CCRYGG 1 cut(s) 522
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 3 cut(s) 585, 590, 639
BstKTI GATC 8 cut(s) 70, 79, 306, 317, 603, 607, 611, 615
BstMAI GTCTC 2 cut(s) 258, 516
BstMBI GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
BstMCI CGRYCG 2 cut(s) 607, 611
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 25, 501
BstX2I RGATCY 3 cut(s) 67, 303, 314
BstYI RGATCY 3 cut(s) 67, 303, 314
Bsu15I ATCGAT 3 cut(s) 603, 607, 611
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 1 cut(s) 321
BsuTUI ATCGAT 3 cut(s) 603, 607, 611
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 3 cut(s) 585, 590, 639
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 117, 595, 655
BveI ACCTGC 2 cut(s) 128, 584
ClaI ATCGAT 3 cut(s) 603, 607, 611
Csp6I GTAC 5 cut(s) 4, 97, 154, 181, 267
CviAII CATG 3 cut(s) 244, 523, 669
CviQI GTAC 5 cut(s) 4, 97, 154, 181, 267
DdeI CTNAG 3 cut(s) 255, 441, 459
DpnI GATC 8 cut(s) 69, 78, 305, 316, 602, 606, 610, 614
DpnII GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
Eco130I CCWWGG 2 cut(s) 322, 522
Eco57I CTGAAG 2 cut(s) 272, 616
Eco81I CCTNAGG 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoT14I CCWWGG 2 cut(s) 322, 522
ErhI CCWWGG 2 cut(s) 322, 522
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 3 cut(s) 247, 526, 672
FaiI YATR 7 cut(s) 75, 245, 524, 557, 567, 653, 670
FatI CATG 3 cut(s) 243, 522, 668
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 2 cut(s) 39, 490
FokI GGATG 3 cut(s) 592, 597, 626
Fsp4HI GCNGC 2 cut(s) 39, 490
FspBI CTAG 1 cut(s) 390
GluI GCNGC 2 cut(s) 39, 490
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 321
HapII CCGG 3 cut(s) 176, 318, 344
Hin1II CATG 3 cut(s) 247, 526, 672
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 4 cut(s) 127, 215, 329, 471
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 3 cut(s) 176, 318, 344
HphI GGTGA 3 cut(s) 154, 338, 672
Hpy166II GTNNAC 4 cut(s) 154, 181, 190, 484
Hpy188I TCNGA 2 cut(s) 258, 600
Hpy188III TCNNGA 3 cut(s) 219, 344, 475
Hpy8I GTNNAC 4 cut(s) 154, 181, 190, 484
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 2 cut(s) 151, 252
HpyCH4V TGCA 6 cut(s) 228, 489, 588, 593, 639, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 3 cut(s) 255, 441, 459
Hsp92II CATG 3 cut(s) 247, 526, 672
Kpn2I TCCGGA 1 cut(s) 343
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 2 cut(s) 25, 501
LweI GCATC 3 cut(s) 97, 575, 648
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 160, 326
MalI GATC 8 cut(s) 69, 78, 305, 316, 602, 606, 610, 614
MboI GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
MboII GAAGA 4 cut(s) 224, 278, 619, 622
MflI RGATCY 3 cut(s) 67, 303, 314
MlyI GAGTC 2 cut(s) 121, 323
MmeI TCCRAC 1 cut(s) 527
MnlI CCTC 9 cut(s) 17, 30, 180, 271, 294, 340, 375, 397, 542
MroI TCCGGA 1 cut(s) 343
MseI TTAA 2 cut(s) 189, 453
MspA1I CMGCKG 2 cut(s) 298, 449
MspI CCGG 3 cut(s) 176, 318, 344
MspR9I CCNGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 1 cut(s) 176
NcoI CCATGG 1 cut(s) 522
NdeII GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
NlaIII CATG 3 cut(s) 247, 526, 672
NlaIV GGNNCC 2 cut(s) 305, 421
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 160, 326
PfeI GAWTC 2 cut(s) 215, 471
PkrI GCNGC 2 cut(s) 40, 491
Ple19I CGATCG 2 cut(s) 607, 611
PleI GAGTC 2 cut(s) 121, 323
PpsI GAGTC 2 cut(s) 121, 323
PspN4I GGNNCC 2 cut(s) 305, 421
PstI CTGCAG 1 cut(s) 491
PsuI RGATCY 3 cut(s) 67, 303, 314
PvuI CGATCG 2 cut(s) 607, 611
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 5 cut(s) 5, 98, 155, 182, 268
RsaNI GTAC 5 cut(s) 4, 97, 154, 181, 267
SaqAI TTAA 2 cut(s) 189, 453
SatI GCNGC 2 cut(s) 39, 490
Sau3AI GATC 8 cut(s) 67, 76, 303, 314, 600, 604, 608, 612
SchI GAGTC 2 cut(s) 121, 323
ScrFI CCNGG 1 cut(s) 176
SfaNI GCATC 3 cut(s) 97, 575, 648
SfcI CTRYAG 1 cut(s) 487
SmlI CTYRAG 2 cut(s) 62, 308
SmoI CTYRAG 2 cut(s) 62, 308
SsiI CCGC 2 cut(s) 166, 298
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 2 cut(s) 322, 522
TaaI ACNGT 2 cut(s) 151, 252
TaqI TCGA 7 cut(s) 125, 220, 332, 547, 603, 607, 611
TatI WGTACW 2 cut(s) 96, 153
TfiI GAWTC 2 cut(s) 215, 471
Tru1I TTAA 2 cut(s) 189, 453
Tru9I TTAA 2 cut(s) 189, 453
TscAI CASTG 3 cut(s) 117, 595, 662
TseFI GTSAC 2 cut(s) 160, 326
TseI GCWGC 2 cut(s) 38, 489
Tsp45I GTSAC 2 cut(s) 160, 326
TspDTI ATGAA 2 cut(s) 232, 395
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 3 cut(s) 117, 595, 662
XagI CCTNNNNNAGG 1 cut(s) 344
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.