pycom02g05080

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0021154
Physical Location & Seq
Forward (+)
3343450 .. 3344612
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
ATGTCAGCGGAGTCTTTCACTTTCCCTTTGGGCAACAACTCTCCTTCATCTCATGTCGTCGAGCAATCAAAGGTTGTTTTGATAGAGGCTGACAATGTTAAAGTGCCTGTAGAGGTAGCAACAATTCCCGAACAGGATTCAGCAGCTTCATCTTTACCAGCTACTCATGAAGAGGCCTCTCTAACTATCAACCTTCCCCCTACCCCAGCAATTGGAGTAGTGGATATGGACAGTTCGTCAGGGGAGAAAGAGAAGGCCCCTAGCTCGACAACAGAAGCTCACAAGACATATTTCCTTGTTCCTCCTTCTATGCCTGATGCTAGCAAGGAAAAGCTTAGCACACCCTCTCAGAGCAAGCAGCAAAAAGAAAAGGCTTCTTCCTCATCTTCCAACTTACCAGAGCAGCCAACTCCTCTAGCTGTGGTTTATCCCAACATATCCCTCTCTGAGGTCTTGCTCGAACTTCAGAACCTCAAGCAAAAGGTTGACTCCCGGAGCCAGAAGCCATCAGTCCAACAAGCTCAAGATCTAAAACAATCCTTCAAACACTGGTTGGAAGGTGGTTTTGATGTCAACTTCCAACGCGACATGCTTAATCAAGCTGAGAGTACACTTGAGAAGCTGTATGAGGTTAATGAGATCACCAAAGAGCAATTCCAGACCTTTAAATACTTCTTCCAGCTTCTAAAGCAGTTGCAGGAACAATACTTCCAAGCTGCCAAAGAGGCTGAACATATGCAACAAATGAAACTCCAACACTCTGAGTTCTCTACTCTTTTGAAATCTGTTATTGGAGAAGGAACCAAAATTAGGGATAGTATTTCTGTAGTGGACCAACAAATTCAGCAGCTAGAGCAAGAGTTGGCCAAACTGAAAGCTGAGAAAGCTAGCTTGGCTAGTGAACTAGCTGTGAAAGTGGTTGAGGCCCGAAGTGCAAGTCAACAAGTAGATATTTTGGATGCTCAGTTGGTCAACAGCAACATTGCAGTTGAAGAGCCTGAGAGGCTGGAGATTGACATGAAGACTGTATATGCTAGGATTGTTGCCCTAGCTAAGGATGCTCATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

357

Amino Acids

39.47

Weight (kDa)

5.03

Isoelectric Point (pI)

57.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021154)

Species Orthologous Gene IDs
malus_domestica MD02G1064500.v1.1 MD02G1064700.v1.1 MD15G1195200.v1.1
prunus_persica Prupe.7G221300_v2.0.a1
pyrus_communis pycom02g05080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 212
AccII CGCG 1 cut(s) 585
AciI CCGC 1 cut(s) 8
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 1 cut(s) 840
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 1 cut(s) 610
AfiI CCNNNNNNNGG 4 cut(s) 212, 448, 810, 1054
AgsI TTSAA 3 cut(s) 544, 781, 992
AhdI GACNNNNNGTC 1 cut(s) 235
AjuI GAANNNNNNNTTGG 4 cut(s) 638, 670, 875, 907
AloI GAACNNNNNNTCC 2 cut(s) 693, 725
AoxI GGCC 4 cut(s) 174, 255, 864, 924
ApeKI GCWGC 5 cut(s) 143, 358, 403, 716, 847
ApoI RAATTY 1 cut(s) 840
ArsI GACNNNNNNTTYG 2 cut(s) 922, 954
AspS9I GGNCC 3 cut(s) 256, 832, 925
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 1 cut(s) 634
AsuNHI GCTAGC 2 cut(s) 320, 887
AvaII GGWCC 1 cut(s) 832
BalI TGGCCA 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 1028
BbvI GCAGC 5 cut(s) 155, 370, 415, 703, 859
BccI CCATC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 493
BfaI CTAG 9 cut(s) 261, 321, 416, 851, 888, 897, 905, 1035, 1049
BfmI CTRYAG 2 cut(s) 108, 825
BglI GCCNNNNNGGC 2 cut(s) 725, 1003
BglII AGATCT 1 cut(s) 526
BisI GCNGC 5 cut(s) 144, 359, 404, 717, 848
BlpI GCTNAGC 1 cut(s) 335
BlsI GCNGC 5 cut(s) 145, 360, 405, 718, 849
Bme1390I CCNGG 1 cut(s) 493
Bme18I GGWCC 1 cut(s) 832
BmeRI GACNNNNNGTC 1 cut(s) 235
BmgT120I GGNCC 3 cut(s) 256, 832, 925
BmiI GGNNCC 3 cut(s) 258, 497, 802
BmrFI CCNGG 1 cut(s) 493
BmsI GCATC 3 cut(s) 307, 949, 1048
BmtI GCTAGC 2 cut(s) 324, 891
BpiI GAAGAC 1 cut(s) 1028
BpmI CTGGAG 1 cut(s) 1028
Bpu10I CCTNAGC 1 cut(s) 1053
Bpu1102I GCTNAGC 1 cut(s) 335
BpuEI CTTGAG 3 cut(s) 458, 507, 635
BpuMI CCSGG 1 cut(s) 493
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 448, 810, 1054
Bse1I ACTGG 1 cut(s) 554
Bse3DI GCAATG 1 cut(s) 981
BseGI GGATG 2 cut(s) 964, 1063
BseLI CCNNNNNNNGG 4 cut(s) 212, 448, 810, 1054
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 7 cut(s) 362, 438, 594, 753, 870, 977, 990
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 5 cut(s) 155, 370, 415, 703, 859
BseYI CCCAGC 1 cut(s) 205
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 4 cut(s) 176, 257, 866, 926
BsiSI CCGG 1 cut(s) 493
BslI CCNNNNNNNGG 4 cut(s) 212, 448, 810, 1054
BsnI GGCC 4 cut(s) 176, 257, 866, 926
Bsp143I GATC 2 cut(s) 526, 639
Bsp1720I GCTNAGC 1 cut(s) 335
BspACI CCGC 1 cut(s) 8
BspANI GGCC 4 cut(s) 176, 257, 866, 926
BspCNI CTCAG 7 cut(s) 361, 439, 595, 754, 871, 976, 991
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 3 cut(s) 258, 497, 802
BspOI GCTAGC 2 cut(s) 324, 891
BspQI GCTCTTC 1 cut(s) 987
BsrDI GCAATG 1 cut(s) 981
BsrI ACTGG 1 cut(s) 554
BssMI GATC 2 cut(s) 526, 639
Bst4CI ACNGT 2 cut(s) 233, 1027
Bst6I CTCTTC 2 cut(s) 165, 987
BstC8I GCNNGC 3 cut(s) 322, 356, 889
BstDEI CTNAG 9 cut(s) 335, 348, 447, 603, 762, 879, 963, 999, 1053
BstENI CCTNNNNNAGG 2 cut(s) 446, 1052
BstF5I GGATG 2 cut(s) 964, 1063
BstFNI CGCG 1 cut(s) 585
BstKTI GATC 2 cut(s) 529, 642
BstMBI GATC 2 cut(s) 526, 639
BstMWI GCNNNNNNNGC 8 cut(s) 688, 725, 853, 884, 893, 932, 1003, 1058
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 1 cut(s) 491
BstSFI CTRYAG 2 cut(s) 108, 825
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 5 cut(s) 155, 370, 415, 703, 859
BstV2I GAAGAC 1 cut(s) 1028
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 4 cut(s) 176, 257, 866, 926
BtsCI GGATG 2 cut(s) 964, 1063
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 3 cut(s) 322, 356, 889
CciI TCATGA 1 cut(s) 166
Cfr13I GGNCC 3 cut(s) 256, 832, 925
Csp6I GTAC 1 cut(s) 609
CviAII CATG 4 cut(s) 53, 167, 589, 1018
CviQI GTAC 1 cut(s) 609
DdeI CTNAG 9 cut(s) 335, 348, 447, 603, 762, 879, 963, 999, 1053
DpnI GATC 2 cut(s) 528, 641
DpnII GATC 2 cut(s) 526, 639
DraI TTTAAA 1 cut(s) 667
DriI GACNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 2 cut(s) 165, 987
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 2 cut(s) 165, 987
Eco147I AGGCCT 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 449
EcoNI CCTNNNNNAGG 2 cut(s) 446, 1052
EcoO109I RGGNCCY 1 cut(s) 256
FaeI CATG 4 cut(s) 56, 170, 592, 1021
FatI CATG 4 cut(s) 52, 166, 588, 1017
FauNDI CATATG 1 cut(s) 735
Fnu4HI GCNGC 5 cut(s) 144, 359, 404, 717, 848
FokI GGATG 1 cut(s) 971
Fsp4HI GCNGC 5 cut(s) 144, 359, 404, 717, 848
FspBI CTAG 9 cut(s) 261, 321, 416, 851, 888, 897, 905, 1035, 1049
GluI GCNGC 5 cut(s) 144, 359, 404, 717, 848
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 1028
HaeIII GGCC 4 cut(s) 176, 257, 866, 926
HapII CCGG 1 cut(s) 493
Hin1II CATG 4 cut(s) 56, 170, 592, 1021
HincII GTYRAC 4 cut(s) 487, 574, 941, 973
HindII GTYRAC 4 cut(s) 487, 574, 941, 973
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 3 cut(s) 11, 137, 488
HpaII CCGG 1 cut(s) 493
HphI GGTGA 1 cut(s) 634
Hpy166II GTNNAC 7 cut(s) 487, 574, 611, 832, 902, 941, 973
Hpy188I TCNGA 4 cut(s) 351, 448, 468, 763
Hpy188III TCNNGA 4 cut(s) 128, 167, 524, 658
Hpy8I GTNNAC 7 cut(s) 487, 574, 611, 832, 902, 941, 973
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 7 cut(s) 54, 203, 247, 315, 550, 551, 791
HpyCH4III ACNGT 2 cut(s) 233, 1027
HpyCH4V TGCA 4 cut(s) 697, 739, 935, 986
HpyF10VI GCNNNNNNNGC 8 cut(s) 688, 725, 853, 884, 893, 932, 1003, 1058
HpyF3I CTNAG 9 cut(s) 335, 348, 447, 603, 762, 879, 963, 999, 1053
Hsp92II CATG 4 cut(s) 56, 170, 592, 1021
Kzo9I GATC 2 cut(s) 526, 639
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 1 cut(s) 495
Lsp1109I GCAGC 5 cut(s) 155, 370, 415, 703, 859
LweI GCATC 3 cut(s) 307, 949, 1048
MaeI CTAG 9 cut(s) 261, 321, 416, 851, 888, 897, 905, 1035, 1049
MalI GATC 2 cut(s) 528, 641
MboI GATC 2 cut(s) 526, 639
MboII GAAGA 6 cut(s) 182, 369, 378, 667, 1004, 1033
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 526
MlsI TGGCCA 1 cut(s) 866
MluCI AATT 5 cut(s) 123, 210, 653, 807, 840
MluNI TGGCCA 1 cut(s) 866
MlyI GAGTC 2 cut(s) 20, 482
MmeI TCCRAC 5 cut(s) 414, 534, 538, 604, 778
Mox20I TGGCCA 1 cut(s) 866
MscI TGGCCA 1 cut(s) 866
MseI TTAA 4 cut(s) 99, 594, 633, 666
Msp20I TGGCCA 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 1 cut(s) 493
MunI CAATTG 1 cut(s) 210
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 8 cut(s) 688, 725, 853, 884, 893, 932, 1003, 1058
NciI CCSGG 1 cut(s) 493
NdeI CATATG 1 cut(s) 735
NdeII GATC 2 cut(s) 526, 639
NheI GCTAGC 2 cut(s) 320, 887
NlaIII CATG 4 cut(s) 56, 170, 592, 1021
NlaIV GGNNCC 3 cut(s) 258, 497, 802
NspI RCATGY 1 cut(s) 592
PagI TCATGA 1 cut(s) 166
PceI AGGCCT 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 987
PfeI GAWTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 212
PfoI TCCNGGA 1 cut(s) 491
PkrI GCNGC 5 cut(s) 145, 360, 405, 718, 849
PleI GAGTC 2 cut(s) 19, 482
PpsI GAGTC 2 cut(s) 19, 482
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 3 cut(s) 258, 497, 802
PspPI GGNCC 3 cut(s) 256, 832, 925
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 1 cut(s) 610
RsaNI GTAC 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 4 cut(s) 99, 594, 633, 666
SatI GCNGC 5 cut(s) 144, 359, 404, 717, 848
Sau3AI GATC 2 cut(s) 526, 639
Sau96I GGNCC 3 cut(s) 256, 832, 925
SchI GAGTC 2 cut(s) 20, 482
ScrFI CCNGG 1 cut(s) 493
SfaNI GCATC 3 cut(s) 307, 949, 1048
SfcI CTRYAG 2 cut(s) 108, 825
SinI GGWCC 1 cut(s) 832
SmlI CTYRAG 3 cut(s) 473, 522, 614
SmoI CTYRAG 3 cut(s) 473, 522, 614
Sse9I AATT 5 cut(s) 123, 210, 653, 807, 840
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 1 cut(s) 8
SspMI CTAG 9 cut(s) 261, 321, 416, 851, 888, 897, 905, 1035, 1049
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 491
TaaI ACNGT 2 cut(s) 233, 1027
TaqI TCGA 3 cut(s) 60, 266, 459
TasI AATT 5 cut(s) 123, 210, 653, 807, 840
TatI WGTACW 1 cut(s) 608
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 4 cut(s) 99, 594, 633, 666
Tru9I TTAA 4 cut(s) 99, 594, 633, 666
TscAI CASTG 1 cut(s) 554
TseI GCWGC 5 cut(s) 143, 358, 403, 716, 847
TspDTI ATGAA 5 cut(s) 36, 138, 183, 761, 1034
TspRI CASTG 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 832
XagI CCTNNNNNAGG 2 cut(s) 446, 1052
XapI RAATTY 1 cut(s) 840
XceI RCATGY 1 cut(s) 592
XspI CTAG 9 cut(s) 261, 321, 416, 851, 888, 897, 905, 1035, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.