pycom02g05320

glutamate carboxypeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022531
Physical Location & Seq
Forward (+)
3561565 .. 3562938
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05320.2

Sequence Viewer

Length: 615 bp
ATGGTAAGCAGTCGCTTAGATCCTCCGTTGGGAAGCGGAGAATCGGATTTCGCAGCTTTTGTTCATCATATTGGAATTCCAGCAGCTGACATGGCTTTCGGTAAAGGTCTTTGGCTAGCTGATGCAGAAGTCTTGCCTTTTAATTATCTCTCCTATGCACTGGAGCTACAGAGTTACGTGAAGGACCTGGAAGATGAGCTTTCAGGTAAGAACGTAAACCTAACTCCTCTGTTCAAGTCCATTGAGGAGCTCAAGAAAGCAGCCACAAATATAAACAGCCAGAGAGAGGAAATAGAACAACGCAAAGCTAATCTATTTACCAGAAAGAAAGATCACCTTAAGGTGAGAGAGCTGAATGACAGACTTATGATGGCAGAGCGCACATTTACAGATCAAGACGGACTCTTAGGAAGGTCATGGTTTAAGCATTTGATTTATGGGCCCTCAAAGCATGATGATTATGGATCTGTATCCTTCCCGGGCATAGGTGAAGCCATCGAAAAGGCAAAGAGTTTGAAGACAGCAGAGTCATGGAAAATTGTACAACATGAAGTTTATAGAGTCTCTAGAGCTGTTAAACATGCATCACAAATCATCAACGGCGAATTAACATGA

Protein Analysis

205

Amino Acids

23.01

Weight (kDa)

6.39

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022531)

Species Orthologous Gene IDs
pyrus_communis pycom02g05320
rosa_chinensis RchiOBHm_Chr5g0038921
rosa_rugosa Rorug05G0175200
rosa_samantha Rh5BG266600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 526
AciI CCGC 1 cut(s) 36
AclWI GGATC 2 cut(s) 14, 472
AcsI RAATTY 1 cut(s) 75
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 3 cut(s) 29, 286, 485
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 2 cut(s) 235, 517
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 9 cut(s) 56, 86, 119, 166, 199, 250, 308, 352, 572
AluI AGCT 9 cut(s) 56, 86, 119, 166, 199, 250, 308, 352, 572
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 2 cut(s) 14, 472
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 1 cut(s) 440
ApaI GGGCCC 1 cut(s) 444
ApeKI GCWGC 3 cut(s) 53, 83, 260
ApoI RAATTY 1 cut(s) 75
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 3 cut(s) 184, 440, 441
AsuC2I CCSGG 2 cut(s) 479, 480
AsuHPI GGTGA 3 cut(s) 326, 355, 500
AsuNHI GCTAGC 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 444
BanII GRGCYC 2 cut(s) 252, 444
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 65, 95, 272
BccI CCATC 2 cut(s) 364, 503
BciT130I CCWGG 1 cut(s) 188
BciVI GTATCC 1 cut(s) 481
BcnI CCSGG 2 cut(s) 479, 480
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 2 cut(s) 116, 567
BfmI CTRYAG 1 cut(s) 167
BfrI CTTAAG 1 cut(s) 338
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 3 cut(s) 54, 84, 261
BlsI GCNGC 3 cut(s) 55, 85, 262
Bme1390I CCNGG 3 cut(s) 188, 479, 480
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 3 cut(s) 184, 440, 441
BmiI GGNNCC 1 cut(s) 442
BmrFI CCNGG 3 cut(s) 188, 479, 480
BmsI GCATC 2 cut(s) 112, 593
BmtI GCTAGC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 524
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 479, 480
BsaAI YACGTR 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 286, 485
Bse1I ACTGG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 469
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 478
BseJI GATNNNNATC 1 cut(s) 469
BseLI CCNNNNNNNGG 3 cut(s) 29, 286, 485
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 2 cut(s) 216, 260
BseSI GKGCMC 1 cut(s) 444
BseXI GCAGC 3 cut(s) 65, 95, 272
BshFI GGCC 1 cut(s) 442
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 1 cut(s) 479
BslI CCNNNNNNNGG 3 cut(s) 29, 286, 485
BsmAI GTCTC 1 cut(s) 568
BsnI GGCC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 478
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 252, 444
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 4 cut(s) 19, 331, 391, 464
BspACI CCGC 1 cut(s) 36
BspANI GGCC 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 442
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 2 cut(s) 14, 472
BspTI CTTAAG 1 cut(s) 338
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 4 cut(s) 19, 331, 391, 464
Bst2UI CCWGG 1 cut(s) 188
BstAFI CTTAAG 1 cut(s) 338
BstAUI TGTACA 1 cut(s) 541
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 16, 406
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 4 cut(s) 22, 334, 394, 467
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 4 cut(s) 19, 331, 391, 464
BstMWI GCNNNNNNNGC 2 cut(s) 92, 448
BstNI CCWGG 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 3 cut(s) 186, 477, 478
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 444
BstV1I GCAGC 3 cut(s) 65, 95, 272
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 19, 464
BstYI RGATCY 2 cut(s) 19, 464
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 117
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 381
Cfr13I GGNCC 3 cut(s) 184, 440, 441
Cfr9I CCCGGG 1 cut(s) 478
Csp6I GTAC 1 cut(s) 542
CviAII CATG 7 cut(s) 91, 417, 452, 531, 548, 581, 612
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 2 cut(s) 16, 406
DpnI GATC 4 cut(s) 21, 333, 393, 466
DpnII GATC 4 cut(s) 19, 331, 391, 464
DrdI GACNNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 526
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 2 cut(s) 252, 444
Eco47I GGWCC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 478
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 2 cut(s) 184, 441
EcoRI GAATTC 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 2 cut(s) 252, 444
FaeI CATG 7 cut(s) 94, 420, 455, 534, 551, 584, 615
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 321, 353
FatI CATG 7 cut(s) 90, 416, 451, 530, 547, 580, 611
Fnu4HI GCNGC 3 cut(s) 54, 84, 261
FriOI GRGCYC 2 cut(s) 252, 444
Fsp4HI GCNGC 3 cut(s) 54, 84, 261
FspBI CTAG 2 cut(s) 116, 567
GlaI GCGC 1 cut(s) 380
GluI GCNGC 3 cut(s) 54, 84, 261
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 1 cut(s) 442
HapII CCGG 1 cut(s) 479
HhaI GCGC 1 cut(s) 381
Hin1II CATG 7 cut(s) 94, 420, 455, 534, 551, 584, 615
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HinfI GANTC 4 cut(s) 41, 402, 527, 561
HpaII CCGG 1 cut(s) 479
HphI GGTGA 3 cut(s) 326, 355, 500
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 3 cut(s) 253, 395, 567
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 3 cut(s) 175, 405, 484
HpyCH4IV ACGT 2 cut(s) 177, 213
HpyCH4V TGCA 3 cut(s) 125, 158, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 448
HpyF3I CTNAG 2 cut(s) 16, 406
HpySE526I ACGT 2 cut(s) 177, 213
Hsp92II CATG 7 cut(s) 94, 420, 455, 534, 551, 584, 615
HspAI GCGC 1 cut(s) 379
Kzo9I GATC 4 cut(s) 19, 331, 391, 464
LmnI GCTCC 2 cut(s) 163, 247
LpnPI CCDG 8 cut(s) 93, 146, 173, 189, 200, 293, 334, 492
Lsp1109I GCAGC 3 cut(s) 65, 95, 272
LweI GCATC 2 cut(s) 112, 593
MaeI CTAG 2 cut(s) 116, 567
MaeII ACGT 2 cut(s) 177, 213
MaeIII GTNAC 1 cut(s) 173
MalI GATC 4 cut(s) 21, 333, 393, 466
MboI GATC 4 cut(s) 19, 331, 391, 464
MboII GAAGA 2 cut(s) 203, 529
MflI RGATCY 2 cut(s) 19, 464
MhlI GDGCHC 2 cut(s) 252, 444
MluCI AATT 4 cut(s) 75, 142, 537, 605
MlyI GAGTC 3 cut(s) 396, 536, 570
MnlI CCTC 5 cut(s) 33, 237, 238, 280, 454
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 5 cut(s) 141, 339, 423, 576, 608
MspA1I CMGCKG 1 cut(s) 86
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 3 cut(s) 188, 479, 480
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 92, 448
NciI CCSGG 2 cut(s) 479, 480
NdeII GATC 4 cut(s) 19, 331, 391, 464
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 7 cut(s) 94, 420, 455, 534, 551, 584, 615
NlaIV GGNNCC 1 cut(s) 442
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 1 cut(s) 584
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 3 cut(s) 55, 85, 262
PleI GAGTC 3 cut(s) 396, 535, 569
PpsI GAGTC 3 cut(s) 396, 535, 569
Ppu21I YACGTR 1 cut(s) 178
PpuMI RGGWCCY 1 cut(s) 184
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 442
PspOMI GGGCCC 1 cut(s) 440
PspPI GGNCC 3 cut(s) 184, 440, 441
PspPPI RGGWCCY 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 19, 464
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 5 cut(s) 141, 339, 423, 576, 608
SatI GCNGC 3 cut(s) 54, 84, 261
Sau3AI GATC 4 cut(s) 19, 331, 391, 464
Sau96I GGNCC 3 cut(s) 184, 440, 441
SchI GAGTC 3 cut(s) 396, 536, 570
ScrFI CCNGG 3 cut(s) 188, 479, 480
SduI GDGCHC 2 cut(s) 252, 444
SfaNI GCATC 2 cut(s) 112, 593
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 184
SmaI CCCGGG 1 cut(s) 480
SmlI CTYRAG 2 cut(s) 251, 338
SmoI CTYRAG 2 cut(s) 251, 338
Sse9I AATT 4 cut(s) 75, 142, 537, 605
SsiI CCGC 1 cut(s) 36
SspMI CTAG 2 cut(s) 116, 567
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 186, 477, 478
TaiI ACGT 2 cut(s) 180, 216
TaqI TCGA 1 cut(s) 498
TasI AATT 4 cut(s) 75, 142, 537, 605
TatI WGTACW 1 cut(s) 541
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 5 cut(s) 141, 339, 423, 576, 608
Tru9I TTAA 5 cut(s) 141, 339, 423, 576, 608
TscAI CASTG 1 cut(s) 165
TseI GCWGC 3 cut(s) 53, 83, 260
TspDTI ATGAA 2 cut(s) 53, 564
TspGWI ACGGA 2 cut(s) 15, 414
TspMI CCCGGG 1 cut(s) 478
TspRI CASTG 1 cut(s) 165
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 1 cut(s) 75
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 1 cut(s) 584
XmaI CCCGGG 1 cut(s) 478
XspI CTAG 2 cut(s) 116, 567
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.