pycom02g07580

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013113
Physical Location & Seq
Forward (+)
5298857 .. 5303998
5142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1476 bp
ATGTTGGAGGCCTTGCCGCTAGGACGGTTCCACCACCAGAGGCTTGACCTGAAGCGGTGGTTTCCCACCTTCTTGTCCTCCCACAAAACCCTCTTCACCGTGCTCTGGATCGCCGCTTTCGCCTCGGTTTTCGTGTGGCAGAGGAACATTGTAGATGGATTTTCGATATTCAGGCGAGCTCCGGCGAGGGCGATGCCGAAGCTGAGGCCGGTGGCTTTCAATTTGACGGAATTTGGGGCTGTGGGCGATGGGGTTACTTTGAACACTGAGGCGTTTGAGAAGGCGGTGCTGGCTATTTCTAAGCTGGGGAAGAAAGGCGGTGGGCAGCTCAATGTGCCTCCGGGACGGTGGCTAACGGCGCCGTTTAACCTCACTAGTCACATGACTCTGTTTCTTGCTGAAGATGCTGAGATACTTGGAATTCAGGATGAGAAGTATTGGACCTTGATGCCACCGTTGCCTTCATACGGGTATGGGAGAGAGCATCATGGGCCTCGGTATGGAAGTCTGATTCATGGTCAAAATCTTAAAGATATTGTTATAACAGGGCATAATGGTACTATAAATGGACAGGGTCAAACATGGTGGAAGAAGTACCGCCAGAAGCTTCTCAACAACACTCGGGGTCCACTTGTTCAGATTATGTGGTCTAGCGACATTTTGATCAGTAATATAACATTGCGTGATTCTCCTTTTTGGACACTTCATCCATATGACTGCAAGAACATTACTGTTAGAAATGTTACAATTTTGGCTCCCGTACTTGAAGCTCCAAATACTGATGGAATAGATCCTGATTCATGTGAGGATATGGTGATTGAGGACTGCTACATTAGTGTAGGGGATGATGGGATTGCAATAAAGAGTGGCTGGGATCAGTATGGTATTTCTTATGGACGGCCATCGAAGAATATACTCATTAGAAATCTTGTTGTCCGCTCTATGGTCAGTGCTGGCGTATCAATTGGCAGTGAGATGTCTGGTGGGGTTTCGAATGTCACTGTGGAGAACCTGCTTGTGTGGAGCTCAAGGCGTGCTGTTCGGATCAAGACTGCCCCTGGAAGAGGCGGATATGTGCGACAAATAACCTATAGGAATCTGACATTTGACAATGTTCGAGTTGGAATTGTTATCAAGACAGACTACAACGAGCACCCCGATGAGGGGTATGATCGAAAGGCTCTTCCAGTGCTTGAGGACATAAGCTTCACAAGCGTCCATGGACAGGGAGTCCGTGTGCCTGTCCGTATCCATGGGAGTGAAGAGATTCCAGTGAGAAATGTGACTTTCCGGGACATGTCCGTTGGGCTAACATACAAAAAGAAGCACATTTTCCAATGTGCCTTTGTTCAAGGTCGTGTGATAGGGACCATCTTCCCTGCCCCATGCGAGAATCTTGACAGGTACGACGAGCAAGGAAAGCTGGTGAATCACTCTGCATCCCAGAACATGACAGACATAGATTATGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

492

Amino Acids

55.11

Weight (kDa)

8.9

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 168 - 450 2.1e-47 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19170 AT3G42950
fragaria_vesca FvH4_1g09050 FvH4_1g09050
malus_domestica MD02G1096000.v1.1
prunus_persica Prupe.7G195000_v2.0.a1
pyrus_communis pycom02g07580 pycom15g19530
rosa_chinensis RchiOBHm_Chr2g0095471
rosa_laevigata RLG00000016570
rosa_multiflora Rmu_sc0006076.1_g000011
rosa_roxburghii Rroxscaffold_2G00146310
rosa_rugosa Rorug02G0051400.1
rosa_samantha Rh2AG099400 Rh2BG100700 Rh2CG102800 Rh2DG100300
rosa_wichuraiana Rw2G007530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc36I ACCTGC 1 cut(s) 1020
AccB1I GGYRCC 1 cut(s) 358
AccBSI CCGCTC 1 cut(s) 939
AciI CCGC 8 cut(s) 17, 55, 114, 284, 318, 598, 937, 1068
AclWI GGATC 4 cut(s) 116, 785, 882, 1052
AcoI YGGCCR 1 cut(s) 899
AcsI RAATTY 2 cut(s) 230, 420
AcuI CTGAAG 2 cut(s) 71, 420
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 4 cut(s) 559, 596, 762, 1406
AfiI CCNNNNNNNGG 9 cut(s) 105, 467, 500, 943, 1064, 1162, 1163, 1164, 1225
AflIII ACRYGT 1 cut(s) 1296
AgsI TTSAA 4 cut(s) 220, 262, 767, 1352
AhdI GACNNNNNGTC 1 cut(s) 1229
AhlI ACTAGT 1 cut(s) 374
AjnI CCWGG 1 cut(s) 1057
AluBI AGCT 9 cut(s) 179, 202, 304, 328, 607, 770, 1026, 1206, 1423
AluI AGCT 9 cut(s) 179, 202, 304, 328, 607, 770, 1026, 1206, 1423
Alw21I GWGCWC 4 cut(s) 105, 181, 1028, 1155
AlwI GGATC 4 cut(s) 116, 785, 882, 1052
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 4 cut(s) 9, 206, 491, 899
ApeKI GCWGC 1 cut(s) 325
ApoI RAATTY 2 cut(s) 230, 420
Asp700I GAANNNNTTC 1 cut(s) 1266
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 4 cut(s) 441, 491, 626, 1368
AsuC2I CCSGG 2 cut(s) 342, 1292
AsuHPI GGTGA 3 cut(s) 88, 826, 1438
AsuII TTCGAA 1 cut(s) 992
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 3 cut(s) 441, 626, 1368
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 181, 1028
Bbv12I GWGCWC 4 cut(s) 105, 181, 1028, 1155
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 1 cut(s) 337
BccI CCATC 6 cut(s) 149, 242, 776, 842, 910, 1379
BceAI ACGGC 3 cut(s) 346, 372, 914
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BciT130I CCWGG 1 cut(s) 1059
BciVI GTATCC 1 cut(s) 1259
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 2 cut(s) 342, 1292
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 3 cut(s) 20, 375, 651
BfmI CTRYAG 1 cut(s) 1090
BfoI RGCGCY 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 1020
BfuI GTATCC 1 cut(s) 1259
BisI GCNGC 3 cut(s) 17, 114, 326
BlsI GCNGC 3 cut(s) 18, 115, 327
Bme1390I CCNGG 3 cut(s) 342, 1059, 1292
Bme18I GGWCC 3 cut(s) 441, 626, 1368
BmeRI GACNNNNNGTC 1 cut(s) 1229
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 4 cut(s) 441, 491, 626, 1368
BmiI GGNNCC 5 cut(s) 29, 360, 627, 756, 1369
BmrFI CCNGG 3 cut(s) 342, 1059, 1292
BmsI GCATC 5 cut(s) 183, 394, 438, 493, 1448
Bpu10I CCTNAGC 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 992
BpuEI CTTGAG 2 cut(s) 1012, 1214
BpuMI CCSGG 2 cut(s) 342, 1292
BsaHI GRCGYC 1 cut(s) 359
BsaJI CCNNGG 5 cut(s) 123, 494, 1057, 1219, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 1221, 1251
Bsc4I CCNNNNNNNGG 9 cut(s) 105, 467, 500, 943, 1064, 1162, 1163, 1164, 1225
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 1187, 1271
Bse3DI GCAATG 1 cut(s) 677
BseBI CCWGG 1 cut(s) 1059
BseDI CCNNGG 5 cut(s) 123, 494, 1057, 1219, 1252
BseGI GGATG 4 cut(s) 433, 706, 850, 1439
BseLI CCNNNNNNNGG 9 cut(s) 105, 467, 500, 943, 1064, 1162, 1163, 1164, 1225
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 3 cut(s) 194, 258, 399
BseNI ACTGG 2 cut(s) 1187, 1271
BseXI GCAGC 1 cut(s) 337
BseYI CCCAGC 2 cut(s) 304, 870
BshFI GGCC 4 cut(s) 11, 208, 493, 901
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 4 cut(s) 105, 181, 1028, 1155
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 4 cut(s) 182, 209, 341, 1291
BslFI GGGAC 3 cut(s) 357, 1307, 1381
BslI CCNNNNNNNGG 9 cut(s) 105, 467, 500, 943, 1064, 1162, 1163, 1164, 1225
BsmFI GGGAC 3 cut(s) 357, 1307, 1381
BsnI GGCC 4 cut(s) 11, 208, 493, 901
BsoBI CYCGRG 1 cut(s) 621
Bsp119I TTCGAA 1 cut(s) 992
Bsp1286I GDGCHC 4 cut(s) 105, 181, 1028, 1155
Bsp143I GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
Bsp19I CCATGG 2 cut(s) 1219, 1252
BspACI CCGC 8 cut(s) 17, 55, 114, 284, 318, 598, 937, 1068
BspANI GGCC 4 cut(s) 11, 208, 493, 901
BspCNI CTCAG 3 cut(s) 195, 259, 400
BspLI GGNNCC 5 cut(s) 29, 360, 627, 756, 1369
BspMI ACCTGC 1 cut(s) 1020
BspPI GGATC 4 cut(s) 116, 785, 882, 1052
BspQI GCTCTTC 1 cut(s) 1188
BspT104I TTCGAA 1 cut(s) 992
BspT107I GGYRCC 1 cut(s) 358
BsrBI CCGCTC 1 cut(s) 939
BsrDI GCAATG 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 2 cut(s) 1187, 1271
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 5 cut(s) 123, 494, 1057, 1219, 1252
BssMI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 2 cut(s) 1219, 1252
Bst2UI CCWGG 1 cut(s) 1059
Bst4CI ACNGT 6 cut(s) 27, 100, 348, 456, 733, 1003
Bst6I CTCTTC 4 cut(s) 98, 1057, 1188, 1257
BstACI GRCGYC 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 992
BstC8I GCNNGC 4 cut(s) 177, 291, 955, 1035
BstDEI CTNAG 4 cut(s) 203, 267, 300, 408
BstDSI CCRYGG 2 cut(s) 1219, 1252
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 4 cut(s) 433, 706, 850, 1439
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 6 cut(s) 111, 666, 793, 877, 1047, 1174
BstMBI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
BstMWI GCNNNNNNNGC 9 cut(s) 119, 290, 334, 358, 404, 457, 490, 1212, 1420
BstNI CCWGG 1 cut(s) 1059
BstNSI RCATGY 1 cut(s) 1300
BstSCI CCNGG 3 cut(s) 340, 1057, 1290
BstSFI CTRYAG 1 cut(s) 1090
BstV1I GCAGC 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BsuI GTATCC 1 cut(s) 1259
BsuRI GGCC 4 cut(s) 11, 208, 493, 901
BtgI CCRYGG 2 cut(s) 1219, 1252
BtgZI GCGATG 2 cut(s) 206, 261
BtsCI GGATG 4 cut(s) 433, 706, 850, 1439
BtsI GCAGTG 1 cut(s) 976
BtsIMutI CAGTG 6 cut(s) 264, 955, 976, 999, 1194, 1278
BveI ACCTGC 1 cut(s) 1020
Cac8I GCNNGC 4 cut(s) 177, 291, 955, 1035
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 4 cut(s) 441, 491, 626, 1368
CseI GACGC 1 cut(s) 1204
Csp6I GTAC 4 cut(s) 558, 595, 761, 1405
CspCI CAANNNNNGTGG 2 cut(s) 566, 601
CviQI GTAC 4 cut(s) 558, 595, 761, 1405
DdeI CTNAG 4 cut(s) 203, 267, 300, 408
DinI GGCGCC 1 cut(s) 360
DpnI GATC 6 cut(s) 110, 665, 792, 876, 1046, 1173
DpnII GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
DriI GACNNNNNGTC 1 cut(s) 1229
EaeI YGGCCR 1 cut(s) 899
Eam1104I CTCTTC 4 cut(s) 98, 1057, 1188, 1257
Eam1105I GACNNNNNGTC 1 cut(s) 1229
EarI CTCTTC 4 cut(s) 98, 1057, 1188, 1257
EciI GGCGGA 1 cut(s) 1083
Ecl136II GAGCTC 2 cut(s) 179, 1026
Eco130I CCWWGG 2 cut(s) 1219, 1252
Eco147I AGGCCT 1 cut(s) 11
Eco24I GRGCYC 2 cut(s) 181, 1028
Eco47I GGWCC 3 cut(s) 441, 626, 1368
Eco53kI GAGCTC 2 cut(s) 179, 1026
Eco57I CTGAAG 2 cut(s) 71, 420
Eco88I CYCGRG 1 cut(s) 621
EcoICRI GAGCTC 2 cut(s) 179, 1026
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 1057
EcoT14I CCWWGG 2 cut(s) 1219, 1252
EcoT38I GRGCYC 2 cut(s) 181, 1028
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 1219, 1252
FaqI GGGAC 3 cut(s) 357, 1307, 1381
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 3 cut(s) 17, 114, 326
FokI GGATG 4 cut(s) 440, 693, 857, 1426
FriOI GRGCYC 2 cut(s) 181, 1028
Fsp4HI GCNGC 3 cut(s) 17, 114, 326
FspBI CTAG 3 cut(s) 20, 375, 651
GlaI GCGC 1 cut(s) 360
GluI GCNGC 3 cut(s) 17, 114, 326
GsaI CCCAGC 2 cut(s) 308, 874
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 4 cut(s) 11, 208, 493, 901
HapII CCGG 4 cut(s) 182, 209, 341, 1291
HgaI GACGC 1 cut(s) 1204
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 359
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HindIII AAGCTT 2 cut(s) 605, 1204
HinfI GANTC 9 cut(s) 385, 511, 686, 797, 1096, 1230, 1267, 1393, 1429
HpaII CCGG 4 cut(s) 182, 209, 341, 1291
HphI GGTGA 3 cut(s) 88, 826, 1438
Hpy166II GTNNAC 1 cut(s) 629
Hpy188I TCNGA 4 cut(s) 510, 639, 1044, 1101
Hpy188III TCNNGA 6 cut(s) 106, 425, 794, 1048, 1135, 1397
Hpy8I GTNNAC 1 cut(s) 629
Hpy99I CGWCG 1 cut(s) 1412
HpyAV CCTTC 3 cut(s) 79, 274, 471
HpyCH4III ACNGT 6 cut(s) 27, 100, 348, 456, 733, 1003
HpyCH4V TGCA 3 cut(s) 720, 857, 1439
HpyF10VI GCNNNNNNNGC 9 cut(s) 119, 290, 334, 358, 404, 457, 490, 1212, 1420
HpyF3I CTNAG 4 cut(s) 203, 267, 300, 408
Hsp92I GRCGYC 1 cut(s) 359
HspAI GCGC 1 cut(s) 359
KasI GGCGCC 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 4 cut(s) 184, 760, 775, 1023
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 5 cut(s) 183, 394, 438, 493, 1448
MaeI CTAG 3 cut(s) 20, 375, 651
MaeIII GTNAC 5 cut(s) 253, 377, 742, 997, 1282
MalI GATC 6 cut(s) 110, 665, 792, 876, 1046, 1173
MbiI CCGCTC 1 cut(s) 939
MboI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
MboII GAAGA 9 cut(s) 85, 322, 413, 601, 919, 1074, 1175, 1274, 1366
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 4 cut(s) 105, 181, 1028, 1155
MluCI AATT 6 cut(s) 220, 230, 420, 747, 963, 1125
Mly113I GGCGCC 1 cut(s) 359
MlyI GAGTC 2 cut(s) 379, 1239
MmeI TCCRAC 1 cut(s) 1102
MroXI GAANNNNTTC 1 cut(s) 1266
MseI TTAA 2 cut(s) 366, 528
MslI CAYNNNNRTG 3 cut(s) 711, 1158, 1257
MspI CCGG 4 cut(s) 182, 209, 341, 1291
MspR9I CCNGG 3 cut(s) 342, 1059, 1292
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 1 cut(s) 1059
MwoI GCNNNNNNNGC 9 cut(s) 119, 290, 334, 358, 404, 457, 490, 1212, 1420
NarI GGCGCC 1 cut(s) 359
NciI CCSGG 2 cut(s) 342, 1292
NcoI CCATGG 2 cut(s) 1219, 1252
NdeI CATATG 1 cut(s) 712
NdeII GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
NlaIV GGNNCC 5 cut(s) 29, 360, 627, 756, 1369
NmuCI GTSAC 3 cut(s) 377, 997, 1282
NspI RCATGY 1 cut(s) 1300
NspV TTCGAA 1 cut(s) 992
PceI AGGCCT 1 cut(s) 11
PciI ACATGT 1 cut(s) 1296
PciSI GCTCTTC 1 cut(s) 1188
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1266
PfeI GAWTC 7 cut(s) 511, 686, 797, 1096, 1267, 1393, 1429
PflFI GACNNNGTC 1 cut(s) 573
PfoI TCCNGGA 2 cut(s) 340, 1290
PkrI GCNGC 3 cut(s) 18, 115, 327
PleI GAGTC 2 cut(s) 379, 1238
PluTI GGCGCC 1 cut(s) 362
PpsI GAGTC 2 cut(s) 379, 1238
PscI ACATGT 1 cut(s) 1296
PsiI TTATAA 1 cut(s) 542
Psp124BI GAGCTC 2 cut(s) 181, 1028
Psp6I CCWGG 1 cut(s) 1057
PspFI CCCAGC 2 cut(s) 304, 870
PspGI CCWGG 1 cut(s) 1057
PspN4I GGNNCC 5 cut(s) 29, 360, 627, 756, 1369
PspPI GGNCC 4 cut(s) 441, 491, 626, 1368
PsuI RGATCY 1 cut(s) 790
PsyI GACNNNGTC 1 cut(s) 573
RsaI GTAC 4 cut(s) 559, 596, 762, 1406
RsaNI GTAC 4 cut(s) 558, 595, 761, 1405
RseI CAYNNNNRTG 3 cut(s) 711, 1158, 1257
SacI GAGCTC 2 cut(s) 181, 1028
SapI GCTCTTC 1 cut(s) 1188
SaqAI TTAA 2 cut(s) 366, 528
SatI GCNGC 3 cut(s) 17, 114, 326
Sau3AI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
Sau96I GGNCC 4 cut(s) 441, 491, 626, 1368
SchI GAGTC 2 cut(s) 379, 1239
ScrFI CCNGG 3 cut(s) 342, 1059, 1292
SduI GDGCHC 4 cut(s) 105, 181, 1028, 1155
SfaNI GCATC 5 cut(s) 183, 394, 438, 493, 1448
SfcI CTRYAG 1 cut(s) 1090
SfoI GGCGCC 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 992
SinI GGWCC 3 cut(s) 441, 626, 1368
SmiMI CAYNNNNRTG 3 cut(s) 711, 1158, 1257
SmlI CTYRAG 2 cut(s) 1027, 1193
SmoI CTYRAG 2 cut(s) 1027, 1193
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 6 cut(s) 220, 230, 420, 747, 963, 1125
SseBI AGGCCT 1 cut(s) 11
SsiI CCGC 8 cut(s) 17, 55, 114, 284, 318, 598, 937, 1068
SspDI GGCGCC 1 cut(s) 358
SspMI CTAG 3 cut(s) 20, 375, 651
SstI GAGCTC 2 cut(s) 181, 1028
StuI AGGCCT 1 cut(s) 11
StyD4I CCNGG 3 cut(s) 340, 1057, 1290
StyI CCWWGG 2 cut(s) 1219, 1252
TaaI ACNGT 6 cut(s) 27, 100, 348, 456, 733, 1003
TaqI TCGA 5 cut(s) 164, 905, 992, 1117, 1174
TasI AATT 6 cut(s) 220, 230, 420, 747, 963, 1125
TauI GCSGC 2 cut(s) 19, 116
TfiI GAWTC 7 cut(s) 511, 686, 797, 1096, 1267, 1393, 1429
Tru1I TTAA 2 cut(s) 366, 528
Tru9I TTAA 2 cut(s) 366, 528
TscAI CASTG 6 cut(s) 271, 955, 976, 1006, 1194, 1278
TseFI GTSAC 3 cut(s) 377, 997, 1282
TseI GCWGC 1 cut(s) 325
Tsp45I GTSAC 3 cut(s) 377, 997, 1282
TspDTI ATGAA 4 cut(s) 453, 503, 695, 789
TspGWI ACGGA 4 cut(s) 242, 1223, 1235, 1291
TspRI CASTG 6 cut(s) 271, 955, 976, 1006, 1194, 1278
Tth111I GACNNNGTC 1 cut(s) 573
VpaK11BI GGWCC 3 cut(s) 441, 626, 1368
XagI CCTNNNNNAGG 1 cut(s) 1062
XapI RAATTY 2 cut(s) 230, 420
XceI RCATGY 1 cut(s) 1300
XmnI GAANNNNTTC 1 cut(s) 1266
XspI CTAG 3 cut(s) 20, 375, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.