pycom02g08630

Vacuolar iron transporter homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016935
Physical Location & Seq
Forward (+)
5970850 .. 5972111
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGAGATGTGACAAGTACATAGGTTTAAACGTTTGGCTTCTGTGTCTTCAACGGAACCACCTAGGTTTTTTTTGGTTGGTTTTCGATCATCAAATGTGGGAGCATAGTCTTTGTCTTTGTCTTTCTTGTGGAAAATTGAAGAAGCCACACTCCCCAAACCCGATTGCTGCGCTAAAATTTTTCCATCTCTTTCCCATGGATACTTCTCATCAAACACTTGAAACCCCGGTTGAACACAAGCTTCCCGTAACTGACGATGAGAATTACACGAAGACCGAGCAAGCGCAGAGAGCTCAGTGGCTCAGAGCTGCTCTTCTGGGAGCAAGCGGCGGGTTGCTGTCAACCATGTCGCTGATGCTTGGTGTTGGGGCAGCTAAGGTAGATCGTTGGACGATGATCTTATCCGGCATAGCGGGAGCTGTTGCAGGAGCTTGTAATATCGAAAAGACTTCTCTTTTAAGACAGAGCAGTTTAAAAACTGAGATGGGTAAGAAAGAAAGGAATGAAAAATCTAACTTAACCATTTCAATTGAACTGACTCCAAGAGTACTCCCATCTGGAAATTCACCATCCAAGCCGCCAGAGCAGCCGGAGAAAGCGAGGAAGATTACTGCCGTTGAGATCACACAAGTTAGTGAAGGGGAGGAGTCATTGCCGAGTCCACACAAGGCAGCATCTGCATCAGCCTTGGCATTTCTGTGCGGCTCACTTGTGCCTTTACTCTCAGCCGTGTTTATTCCTCAAAATACTATAAGAATCGCGGTGGTTGTGATGGTGGCTTCAATAACTCTGGCTTTCTTTGGCGGCGTGGGAGCTCGTATGGGTGGATCCCCGGCCATGCCATCTGCATTTAGGGTTTTAGTTGGAGGGTGGTTGCCGTGGCAGTCACTTATGGCTTGCTTCATGCCATATGAAAAAAAAAGCATATATTTATTGTGCAAGTCGAGAAGATCTTTCGGCATCCCAGTCGTAGTTAAAATTTCTCTCGTGCAAGTCCGGGTTTTCATAGAATTCTCAAGGTTGAATTTAGACTTTTTTGTCTTGTTAAATAACAAATTACAAAGAATGATGAATGTAATCAAGTTTAGAGACGTGAGATTAAATTACATAAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

373

Amino Acids

41.32

Weight (kDa)

9.57

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 101 - 292 5.9e-22 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016935)

Species Orthologous Gene IDs
malus_domestica MD15G1227000.v1.1
prunus_persica Prupe.7G188300_v2.0.a1
pyrus_communis pycom02g08630
rosa_chinensis RchiOBHm_Chr2g0096671
rosa_laevigata RLG00000016660
rosa_multiflora Rmu_sc0003565.1_g000019 Rmu_sc0003565.1_g000020
rosa_roxburghii Rroxscaffold_2G00145430
rosa_rugosa Rorug02G0059500
rosa_samantha Rh2AG106900 Rh2BG109300 Rh2CG111100 Rh2DG110700
rosa_wichuraiana Rw2G008280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
AccII CGCG 1 cut(s) 761
AciI CCGC 7 cut(s) 327, 330, 413, 578, 702, 761, 804
AclI AACGTT 1 cut(s) 30
AclWI GGATC 2 cut(s) 822, 835
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 5 cut(s) 176, 562, 978, 1010, 1024
AfaI GTAC 2 cut(s) 17, 549
AgsI TTSAA 8 cut(s) 50, 139, 221, 233, 528, 533, 783, 1024
AjiI CACGTC 1 cut(s) 1093
AluBI AGCT 7 cut(s) 241, 293, 308, 374, 419, 431, 815
AluI AGCT 7 cut(s) 241, 293, 308, 374, 419, 431, 815
Alw21I GWGCWC 2 cut(s) 295, 817
Alw26I GTCTC 1 cut(s) 1083
AlwI GGATC 2 cut(s) 822, 835
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 1 cut(s) 834
ApeKI GCWGC 5 cut(s) 167, 308, 371, 586, 671
ApoI RAATTY 5 cut(s) 176, 562, 978, 1010, 1024
AspA2I CCTAGG 1 cut(s) 61
AspLEI GCGC 2 cut(s) 172, 286
AsuC2I CCSGG 3 cut(s) 227, 833, 998
AsuHPI GGTGA 1 cut(s) 558
AvrII CCTAGG 1 cut(s) 61
BamHI GGATCC 1 cut(s) 827
BanII GRGCYC 2 cut(s) 295, 817
BauI CACGAG 1 cut(s) 986
BbsI GAAGAC 2 cut(s) 38, 278
Bbv12I GWGCWC 2 cut(s) 295, 817
BbvI GCAGC 5 cut(s) 154, 295, 383, 598, 683
BccI CCATC 6 cut(s) 192, 478, 562, 577, 766, 850
BceAI ACGGC 3 cut(s) 599, 713, 862
BcgI CGANNNNNNTGC 2 cut(s) 949, 983
BciVI GTATCC 1 cut(s) 193
BcnI CCSGG 3 cut(s) 227, 833, 998
BcoDI GTCTC 1 cut(s) 1083
BfaI CTAG 1 cut(s) 62
BfuI GTATCC 1 cut(s) 193
BglII AGATCT 1 cut(s) 950
BisI GCNGC 9 cut(s) 168, 309, 328, 372, 578, 587, 672, 703, 805
BlnI CCTAGG 1 cut(s) 61
BlsI GCNGC 9 cut(s) 169, 310, 329, 373, 579, 588, 673, 704, 806
BmcAI AGTACT 1 cut(s) 549
Bme1390I CCNGG 3 cut(s) 227, 833, 998
BmgBI CACGTC 1 cut(s) 1093
BmiI GGNNCC 2 cut(s) 56, 829
BmrFI CCNGG 3 cut(s) 227, 833, 998
BmrI ACTGGG 1 cut(s) 959
BmsI GCATC 4 cut(s) 345, 683, 689, 969
BmuI ACTGGG 1 cut(s) 959
BpiI GAAGAC 2 cut(s) 38, 278
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 1000
BpuMI CCSGG 3 cut(s) 227, 833, 998
BsaJI CCNNGG 6 cut(s) 61, 195, 225, 687, 831, 878
Bse1I ACTGG 1 cut(s) 965
Bse3DI GCAATG 1 cut(s) 650
BseDI CCNNGG 6 cut(s) 61, 195, 225, 687, 831, 878
BseGI GGATG 2 cut(s) 569, 960
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 4 cut(s) 308, 316, 471, 738
BseNI ACTGG 1 cut(s) 965
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 5 cut(s) 154, 295, 383, 598, 683
Bsh1236I CGCG 1 cut(s) 761
BshFI GGCC 1 cut(s) 836
BsiHKAI GWGCWC 2 cut(s) 295, 817
BsiSI CCGG 5 cut(s) 227, 405, 590, 833, 997
BsmAI GTCTC 1 cut(s) 1083
BsmBI CGTCTC 1 cut(s) 1083
BsnI GGCC 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 295, 817
Bsp143I GATC 6 cut(s) 85, 382, 396, 621, 827, 950
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 7 cut(s) 327, 330, 413, 578, 702, 761, 804
BspANI GGCC 1 cut(s) 836
BspCNI CTCAG 4 cut(s) 307, 315, 472, 737
BspFNI CGCG 1 cut(s) 761
BspLI GGNNCC 2 cut(s) 56, 829
BspPI GGATC 2 cut(s) 822, 835
BspQI GCTCTTC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 650
BsrI ACTGG 1 cut(s) 965
BssECI CCNNGG 6 cut(s) 61, 195, 225, 687, 831, 878
BssMI GATC 6 cut(s) 85, 382, 396, 621, 827, 950
BssSI CACGAG 1 cut(s) 986
BssT1I CCWWGG 3 cut(s) 61, 195, 687
Bst2BI CACGAG 1 cut(s) 986
Bst6I CTCTTC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 677
BstC8I GCNNGC 3 cut(s) 282, 325, 898
BstDEI CTNAG 5 cut(s) 294, 302, 375, 480, 724
BstDSI CCRYGG 2 cut(s) 195, 878
BstF5I GGATG 2 cut(s) 569, 960
BstFNI CGCG 1 cut(s) 761
BstHHI GCGC 2 cut(s) 172, 286
BstKTI GATC 6 cut(s) 88, 385, 399, 624, 830, 953
BstMAI GTCTC 1 cut(s) 1083
BstMBI GATC 6 cut(s) 85, 382, 396, 621, 827, 950
BstMWI GCNNNNNNNGC 4 cut(s) 290, 583, 586, 677
BstSCI CCNGG 3 cut(s) 225, 831, 996
BstUI CGCG 1 cut(s) 761
BstV1I GCAGC 5 cut(s) 154, 295, 383, 598, 683
BstV2I GAAGAC 2 cut(s) 38, 278
BstX2I RGATCY 2 cut(s) 827, 950
BstYI RGATCY 2 cut(s) 827, 950
BsuI GTATCC 1 cut(s) 193
BsuRI GGCC 1 cut(s) 836
BtgI CCRYGG 2 cut(s) 195, 878
BtrI CACGTC 1 cut(s) 1093
BtsCI GGATG 2 cut(s) 569, 960
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 3 cut(s) 282, 325, 898
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 2 cut(s) 172, 286
Csp6I GTAC 2 cut(s) 16, 548
CviAII CATG 4 cut(s) 196, 346, 838, 904
CviQI GTAC 2 cut(s) 16, 548
DdeI CTNAG 5 cut(s) 294, 302, 375, 480, 724
DpnI GATC 6 cut(s) 87, 384, 398, 623, 829, 952
DpnII GATC 6 cut(s) 85, 382, 396, 621, 827, 950
DraI TTTAAA 2 cut(s) 27, 474
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Ecl136II GAGCTC 2 cut(s) 293, 815
Eco130I CCWWGG 3 cut(s) 61, 195, 687
Eco24I GRGCYC 2 cut(s) 295, 817
Eco53kI GAGCTC 2 cut(s) 293, 815
EcoICRI GAGCTC 2 cut(s) 293, 815
EcoRI GAATTC 1 cut(s) 1010
EcoT14I CCWWGG 3 cut(s) 61, 195, 687
EcoT38I GRGCYC 2 cut(s) 295, 817
ErhI CCWWGG 3 cut(s) 61, 195, 687
Esp3I CGTCTC 1 cut(s) 1083
FaeI CATG 4 cut(s) 199, 349, 841, 907
FatI CATG 4 cut(s) 195, 345, 837, 903
FauI CCCGC 2 cut(s) 323, 406
FauNDI CATATG 1 cut(s) 910
Fnu4HI GCNGC 9 cut(s) 168, 309, 328, 372, 578, 587, 672, 703, 805
FokI GGATG 2 cut(s) 556, 947
FriOI GRGCYC 2 cut(s) 295, 817
Fsp4HI GCNGC 9 cut(s) 168, 309, 328, 372, 578, 587, 672, 703, 805
FspBI CTAG 1 cut(s) 62
GlaI GCGC 2 cut(s) 171, 285
GluI GCNGC 9 cut(s) 168, 309, 328, 372, 578, 587, 672, 703, 805
HaeIII GGCC 1 cut(s) 836
HapII CCGG 5 cut(s) 227, 405, 590, 833, 997
HhaI GCGC 2 cut(s) 172, 286
Hin1II CATG 4 cut(s) 199, 349, 841, 907
Hin6I GCGC 2 cut(s) 170, 284
HinP1I GCGC 2 cut(s) 170, 284
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 4 cut(s) 538, 647, 658, 756
HpaII CCGG 5 cut(s) 227, 405, 590, 833, 997
HphI GGTGA 1 cut(s) 558
Hpy166II GTNNAC 2 cut(s) 342, 662
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 2 cut(s) 558, 945
Hpy8I GTNNAC 2 cut(s) 342, 662
HpyAV CCTTC 1 cut(s) 632
HpyCH4IV ACGT 2 cut(s) 30, 1092
HpyCH4V TGCA 5 cut(s) 425, 680, 848, 939, 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 290, 583, 586, 677
HpyF3I CTNAG 5 cut(s) 294, 302, 375, 480, 724
HpySE526I ACGT 2 cut(s) 30, 1092
Hsp92II CATG 4 cut(s) 199, 349, 841, 907
HspAI GCGC 2 cut(s) 170, 284
Kzo9I GATC 6 cut(s) 85, 382, 396, 621, 827, 950
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 5 cut(s) 100, 320, 416, 428, 812
Lsp1109I GCAGC 5 cut(s) 154, 295, 383, 598, 683
LweI GCATC 4 cut(s) 345, 683, 689, 969
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 30, 1092
MaeIII GTNAC 3 cut(s) 8, 247, 885
MalI GATC 6 cut(s) 87, 384, 398, 623, 829, 952
MboI GATC 6 cut(s) 85, 382, 396, 621, 827, 950
MboII GAAGA 6 cut(s) 38, 151, 283, 305, 616, 960
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 2 cut(s) 827, 950
MhlI GDGCHC 2 cut(s) 295, 817
MlyI GAGTC 3 cut(s) 532, 656, 667
MmeI TCCRAC 2 cut(s) 368, 844
MnlI CCTC 4 cut(s) 594, 637, 750, 860
MseI TTAA 7 cut(s) 26, 458, 473, 518, 975, 1046, 1100
MslI CAYNNNNRTG 1 cut(s) 697
MspI CCGG 5 cut(s) 227, 405, 590, 833, 997
MspR9I CCNGG 3 cut(s) 227, 833, 998
MssI GTTTAAAC 1 cut(s) 27
MunI CAATTG 1 cut(s) 528
MvnI CGCG 1 cut(s) 761
MwoI GCNNNNNNNGC 4 cut(s) 290, 583, 586, 677
NciI CCSGG 3 cut(s) 227, 833, 998
NcoI CCATGG 1 cut(s) 195
NdeI CATATG 1 cut(s) 910
NdeII GATC 6 cut(s) 85, 382, 396, 621, 827, 950
NlaIII CATG 4 cut(s) 199, 349, 841, 907
NlaIV GGNNCC 2 cut(s) 56, 829
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 2 cut(s) 8, 885
PciSI GCTCTTC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 756
PkrI GCNGC 9 cut(s) 169, 310, 329, 373, 579, 588, 673, 704, 806
PleI GAGTC 3 cut(s) 532, 655, 666
PmeI GTTTAAAC 1 cut(s) 27
PpsI GAGTC 3 cut(s) 532, 655, 666
Psp124BI GAGCTC 2 cut(s) 295, 817
Psp1406I AACGTT 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 56, 829
PstNI CAGNNNCTG 1 cut(s) 677
PsuI RGATCY 2 cut(s) 827, 950
RsaI GTAC 2 cut(s) 17, 549
RsaNI GTAC 2 cut(s) 16, 548
RseI CAYNNNNRTG 1 cut(s) 697
SacI GAGCTC 2 cut(s) 295, 817
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 7 cut(s) 26, 458, 473, 518, 975, 1046, 1100
SatI GCNGC 9 cut(s) 168, 309, 328, 372, 578, 587, 672, 703, 805
Sau3AI GATC 6 cut(s) 85, 382, 396, 621, 827, 950
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 3 cut(s) 532, 656, 667
ScrFI CCNGG 3 cut(s) 227, 833, 998
SduI GDGCHC 2 cut(s) 295, 817
SfaNI GCATC 4 cut(s) 345, 683, 689, 969
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 1015
SmoI CTYRAG 1 cut(s) 1015
SsiI CCGC 7 cut(s) 327, 330, 413, 578, 702, 761, 804
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 2 cut(s) 295, 817
StyD4I CCNGG 3 cut(s) 225, 831, 996
StyI CCWWGG 3 cut(s) 61, 195, 687
TaiI ACGT 2 cut(s) 33, 1095
TaqI TCGA 3 cut(s) 84, 441, 944
TaqII GACCGA 1 cut(s) 290
TatI WGTACW 2 cut(s) 15, 547
TauI GCSGC 4 cut(s) 330, 580, 705, 807
TfiI GAWTC 1 cut(s) 756
Tru1I TTAA 7 cut(s) 26, 458, 473, 518, 975, 1046, 1100
Tru9I TTAA 7 cut(s) 26, 458, 473, 518, 975, 1046, 1100
TscAI CASTG 1 cut(s) 302
TseFI GTSAC 2 cut(s) 8, 885
TseI GCWGC 5 cut(s) 167, 308, 371, 586, 671
Tsp45I GTSAC 2 cut(s) 8, 885
TspDTI ATGAA 5 cut(s) 519, 892, 927, 994, 1085
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 302
XapI RAATTY 5 cut(s) 176, 562, 978, 1010, 1024
XmaJI CCTAGG 1 cut(s) 61
XspI CTAG 1 cut(s) 62
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.