pycom02g12170
ERF Family

Major Facilitator Superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014169
Physical Location & Seq
Forward (+)
8749255 .. 8752212
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12170.5

Sequence Viewer

Length: 1530 bp
ATGTTAGACATAGTCTTTGAATATCCGTTTAGAAATTTGTTGATGAGCCCGTCGGAGGGCCTTAATTGGGGATTAATCAGATTTCCATCATCTCATACTTCGTCTCGGTCGAAACCTGATTTTTTCAGAAGTTTGGAAGCCATGAGGACAAGAAAGGTTTTTGGGATTTCGCTGTCTCTCATTCTGATCAATTTGGCTGCTATAATGGAGCGCGCTGATGAAAATCTACTCCCATCTGTTTACAAAGAAGTCAGTGAGGCTTTCAATGCCGGGCCGTCTGACCTTGGCTATCTGACATTCATTAGGAACTTTATACAGGGAATGGCATCACCCCTTGCAGGTGTACTAGTTATTAACTATGACCGCCCTGCGGTTCTTGCAATGGGCACTTTTTGCTGGGCTTTGTCAACAGCCGCAGTGGGTGCAAGTCGTTTTTTTTCACAAGTTGCATTCTGGAGGGCAGTGAATGGCTTCGGATTGGCAATTGTTATACCGGCACTTCAGTCGTTTATTGCTGATAGTTATAAGGATGGTGTGAGGGGGGCTGGATTTGGGATGGTAAGCCTTGTCGGTTCCTTGGGTGGAATTGGGGGTGGTGTTCTGGCTACACTCATGGCTGGTGAACAGTACTGGAATATACCGGGATGGCGCTGTGCTTTCATTCTCATGGCATCACTTAGTTCTCTAATTGGGTTCCTTGTTTTCGCGTTTGTGGTTGACCCGAGAAAAATAGTTACTGACCCGACTTTTGAGAGGGAAGATTTGATAATTAAGGGACCTACAAGTGCAGCATCAGTTTGGCTGGAGTCTTGGACAGCCATGAAGGCAGTTGTTAAAGTGCGAACGTTTCAAATAATTGTTTTGCAGGGCGTTGTTGGCTCACTACCCTGGACTGCCATGGTGTTCTTCACAATGTGGTTCCAATTAATCGGCTTTGATCACAGCAGTACGGCAGCCTTGCTTAGTCTTTTTGCTGTTGGATGTGCTGTGGGGTCTCTTCTTGGTGGACTAATAGCAGATCGAATGTCACGGATATATCCTCACTCAGGCCGTATCATGTGTGCGCAGTTCAGCGCCTTCATGGGCATCCCCTTTTCCTGGTTCTTACTCAAAGTCATCCCACAGTCAGTAGACAGCTTTTACACCTTCGCGGTCACCCTCTTGCTGATGGGCCTGACTATCAGCTGGAATGCTACTGCTGCAAATGGTCCTATGTTTGCCGAGGTTGTCCCTGTCAAACACCGGACCATGATCGATCCCATTGATCGAGCTTTCGAAGGATCACTCTCTTCTTTTGCAGCTCCCTTCGTTGGAATCCTTTCAGAGAAGATGTTTGGTTACGATGCAAAATCAGTGGATCCGACTAAAGGGTCTACTCGGGAGGCCTTGGCATTATCACAAGGTCTTCTTTCGATGATGGCAGTTCCGTTTGGTTTGTGTAGCTTGCTGTACACGCCTTTGTATCTGTTTTTCAGAAAGGACCGCGAAAATGCCAGAATCGCCAGTGTCAAAGAGGAAGAGATGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0002165 GO:0003006 GO:0003376 GO:0003674 GO:0005215 GO:0005319 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005770 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0006897 GO:0006915 GO:0006928 GO:0006950 GO:0006996 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007399 GO:0007416 GO:0007417 GO:0007528 GO:0007610 GO:0007617 GO:0007618 GO:0007619 GO:0008150 GO:0008219 GO:0008333 GO:0008347 GO:0008582 GO:0009267 GO:0009605 GO:0009653 GO:0009791 GO:0009886 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010001 GO:0010008 GO:0010623 GO:0010646 GO:0010648 GO:0010876 GO:0010941 GO:0012501 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016477 GO:0019098 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023057 GO:0030154 GO:0031090 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031902 GO:0031982 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033554 GO:0035193 GO:0036465 GO:0040007 GO:0040008 GO:0040011 GO:0042063 GO:0042594 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045476 GO:0045477 GO:0045595 GO:0045924 GO:0046624 GO:0046907 GO:0048468 GO:0048477 GO:0048488 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0048609 GO:0048638 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050803 GO:0050807 GO:0050808 GO:0050896 GO:0051124 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051641 GO:0051649 GO:0051674 GO:0051704 GO:0051716 GO:0051960 GO:0051963 GO:0060180 GO:0060284 GO:0065007 GO:0065008 GO:0071496 GO:0071702 GO:0071840 GO:0080171 GO:0090092 GO:0090097 GO:0090099 GO:0090101 GO:0090520 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0099003 GO:0099504 GO:1902742 GO:1904396 GO:1904748 GO:1905879 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1

Protein Analysis

510

Amino Acids

55.62

Weight (kDa)

8.51

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36790
fragaria_vesca FvH4_1g14050 FvH4_1g14050
malus_domestica MD02G1154100.v1.1 MD15G1268500.v1.1
prunus_persica Prupe.7G148900_v2.0.a1
pyrus_communis pycom02g12170
rosa_chinensis RchiOBHm_Chr2g0102651
rosa_laevigata RLG00000017159
rosa_multiflora Rmu_sc0003852.1_g000017
rosa_roxburghii Rroxscaffold_2G00140420
rosa_rugosa Rorug02G0108300
rosa_samantha Rh2AG157100 Rh2BG163400 Rh2CG162600 Rh2DG162300
rosa_wichuraiana Rw2G012190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AarI CACCTGC 1 cut(s) 329
AasI GACNNNNNNGTC 1 cut(s) 1369
Acc16I TGCGCA 1 cut(s) 1065
Acc36I ACCTGC 1 cut(s) 329
AccI GTMKAC 2 cut(s) 1131, 1373
AccII CGCG 4 cut(s) 213, 707, 1151, 1485
AciI CCGC 5 cut(s) 364, 371, 414, 1151, 1483
AclI AACGTT 1 cut(s) 845
AclWI GGATC 4 cut(s) 1250, 1288, 1352, 1365
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 485
AdeI CACNNNGTG 1 cut(s) 915
AfaI GTAC 4 cut(s) 345, 629, 949, 1451
AfiI CCNNNNNNNGG 9 cut(s) 55, 56, 67, 338, 370, 1046, 1098, 1310, 1367
AgsI TTSAA 3 cut(s) 20, 265, 851
AhlI ACTAGT 1 cut(s) 346
AjiI CACGTC 1 cut(s) 1527
AjnI CCWGG 2 cut(s) 887, 1097
AluBI AGCT 5 cut(s) 1137, 1185, 1271, 1301, 1443
AluI AGCT 5 cut(s) 1137, 1185, 1271, 1301, 1443
Alw26I GTCTC 3 cut(s) 108, 180, 999
AlwI GGATC 4 cut(s) 1250, 1288, 1352, 1365
Ama87I CYCGRG 2 cut(s) 721, 1377
AoxI GGCC 5 cut(s) 58, 272, 1048, 1171, 1383
ApeKI GCWGC 5 cut(s) 197, 788, 953, 1199, 1298
ApoI RAATTY 1 cut(s) 34
ArsI GACNNNNNNTTYG 3 cut(s) 31, 731, 763
AseI ATTAAT 2 cut(s) 74, 926
Asp700I GAANNNNTTC 2 cut(s) 470, 1318
AspLEI GCGC 5 cut(s) 213, 215, 651, 1066, 1076
AspS9I GGNCC 7 cut(s) 58, 272, 776, 1171, 1208, 1245, 1480
AsuC2I CCSGG 2 cut(s) 271, 642
AsuHPI GGTGA 3 cut(s) 321, 632, 1147
AsuII TTCGAA 1 cut(s) 1275
AvaI CYCGRG 2 cut(s) 721, 1377
AvaII GGWCC 4 cut(s) 776, 1208, 1245, 1480
BaeGI GKGCMC 1 cut(s) 389
BamHI GGATCC 1 cut(s) 1357
BanII GRGCYC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 1397
BbvI GCAGC 5 cut(s) 184, 800, 965, 1186, 1310
BccI CCATC 7 cut(s) 94, 241, 524, 550, 639, 1162, 1411
BceAI ACGGC 3 cut(s) 259, 966, 1035
BcgI CGANNNNNNTGC 2 cut(s) 486, 520
BciT130I CCWGG 2 cut(s) 889, 1099
BclI TGATCA 2 cut(s) 186, 937
BcnI CCSGG 2 cut(s) 271, 642
BcoDI GTCTC 3 cut(s) 108, 180, 999
BcuI ACTAGT 1 cut(s) 346
BfaI CTAG 1 cut(s) 347
BfoI RGCGCY 2 cut(s) 652, 1077
BfuAI ACCTGC 1 cut(s) 329
BglI GCCNNNNNGGC 1 cut(s) 824
BisI GCNGC 6 cut(s) 198, 414, 789, 954, 1200, 1299
BlsI GCNGC 6 cut(s) 199, 415, 790, 955, 1201, 1300
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 4 cut(s) 271, 642, 889, 1099
Bme18I GGWCC 4 cut(s) 776, 1208, 1245, 1480
BmeT110I CYCGRG 2 cut(s) 721, 1377
BmgBI CACGTC 1 cut(s) 1527
BmgT120I GGNCC 7 cut(s) 58, 272, 776, 1171, 1208, 1245, 1480
BmiI GGNNCC 5 cut(s) 574, 695, 777, 920, 1359
BmrFI CCNGG 4 cut(s) 271, 642, 889, 1099
BmsI GCATC 5 cut(s) 335, 680, 800, 1095, 1333
BpiI GAAGAC 1 cut(s) 1397
BpmI CTGGAG 2 cut(s) 475, 824
Bpu14I TTCGAA 1 cut(s) 1275
BpuMI CCSGG 2 cut(s) 271, 642
Bsa29I ATCGAT 1 cut(s) 1254
BsaBI GATNNNNATC 2 cut(s) 85, 222
BsaI GGTCTC 1 cut(s) 999
BsaJI CCNNGG 6 cut(s) 283, 576, 887, 897, 1221, 1386
BsaWI WCCGGW 1 cut(s) 1242
Bsc4I CCNNNNNNNGG 9 cut(s) 55, 56, 67, 338, 370, 1046, 1098, 1310, 1367
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 635, 1503
Bse3DI GCAATG 1 cut(s) 387
Bse8I GATNNNNATC 2 cut(s) 85, 222
BseBI CCWGG 2 cut(s) 889, 1099
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 6 cut(s) 283, 576, 887, 897, 1221, 1386
BseGI GGATG 6 cut(s) 535, 561, 650, 986, 1086, 1116
BseJI GATNNNNATC 2 cut(s) 85, 222
BseLI CCNNNNNNNGG 9 cut(s) 55, 56, 67, 338, 370, 1046, 1098, 1310, 1367
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 1059
BseNI ACTGG 2 cut(s) 635, 1503
BsePI GCGCGC 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 5 cut(s) 184, 800, 965, 1186, 1310
BseYI CCCAGC 1 cut(s) 396
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 4 cut(s) 213, 707, 1151, 1485
Bsh1285I CGRYCG 1 cut(s) 110
BshFI GGCC 5 cut(s) 60, 274, 1050, 1173, 1385
BshVI ATCGAT 1 cut(s) 1254
BsiEI CGRYCG 1 cut(s) 110
BsiHKCI CYCGRG 2 cut(s) 721, 1377
BsiSI CCGG 4 cut(s) 270, 494, 641, 1243
BslFI GGGAC 2 cut(s) 789, 1214
BslI CCNNNNNNNGG 9 cut(s) 55, 56, 67, 338, 370, 1046, 1098, 1310, 1367
BsmAI GTCTC 3 cut(s) 108, 180, 999
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 789, 1214
BsmI GAATGC 2 cut(s) 449, 1195
BsnI GGCC 5 cut(s) 60, 274, 1050, 1173, 1385
Bso31I GGTCTC 1 cut(s) 999
BsoBI CYCGRG 2 cut(s) 721, 1377
Bsp119I TTCGAA 1 cut(s) 1275
Bsp1286I GDGCHC 2 cut(s) 50, 389
Bsp1407I TGTACA 1 cut(s) 1449
Bsp143I GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
Bsp19I CCATGG 1 cut(s) 897
BspACI CCGC 5 cut(s) 364, 371, 414, 1151, 1483
BspANI GGCC 5 cut(s) 60, 274, 1050, 1173, 1385
BspCNI CTCAG 1 cut(s) 1058
BspDI ATCGAT 1 cut(s) 1254
BspFNI CGCG 4 cut(s) 213, 707, 1151, 1485
BspLI GGNNCC 5 cut(s) 574, 695, 777, 920, 1359
BspMI ACCTGC 1 cut(s) 329
BspPI GGATC 4 cut(s) 1250, 1288, 1352, 1365
BspT104I TTCGAA 1 cut(s) 1275
BspTNI GGTCTC 1 cut(s) 999
BsrDI GCAATG 1 cut(s) 387
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 1449
BsrI ACTGG 2 cut(s) 635, 1503
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 6 cut(s) 283, 576, 887, 897, 1221, 1386
BssHII GCGCGC 1 cut(s) 211
BssMI GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
BssT1I CCWWGG 4 cut(s) 283, 576, 897, 1386
Bst2UI CCWGG 2 cut(s) 889, 1099
Bst4CI ACNGT 2 cut(s) 627, 1125
Bst6I CTCTTC 3 cut(s) 1002, 1294, 1512
BstAPI GCANNNNNTGC 2 cut(s) 393, 422
BstAUI TGTACA 1 cut(s) 1449
BstBI TTCGAA 1 cut(s) 1275
BstC8I GCNNGC 2 cut(s) 213, 1445
BstDEI CTNAG 3 cut(s) 677, 962, 1045
BstDSI CCRYGG 1 cut(s) 897
BstEII GGTNACC 1 cut(s) 1153
BstENI CCTNNNNNAGG 1 cut(s) 1044
BstF5I GGATG 6 cut(s) 535, 561, 650, 986, 1086, 1116
BstFNI CGCG 4 cut(s) 213, 707, 1151, 1485
BstH2I RGCGCY 2 cut(s) 652, 1077
BstHHI GCGC 5 cut(s) 213, 215, 651, 1066, 1076
BstKTI GATC 8 cut(s) 189, 940, 1021, 1254, 1258, 1267, 1283, 1360
BstMAI GTCTC 3 cut(s) 108, 180, 999
BstMBI GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
BstMCI CGRYCG 1 cut(s) 110
BstMWI GCNNNNNNNGC 9 cut(s) 266, 377, 393, 422, 824, 876, 1199, 1453, 1499
BstNI CCWGG 2 cut(s) 889, 1099
BstPI GGTNACC 1 cut(s) 1153
BstSCI CCNGG 4 cut(s) 269, 640, 887, 1097
BstSLI GKGCMC 1 cut(s) 389
BstUI CGCG 4 cut(s) 213, 707, 1151, 1485
BstV1I GCAGC 5 cut(s) 184, 800, 965, 1186, 1310
BstV2I GAAGAC 1 cut(s) 1397
BstX2I RGATCY 1 cut(s) 1357
BstYI RGATCY 1 cut(s) 1357
Bsu15I ATCGAT 1 cut(s) 1254
BsuRI GGCC 5 cut(s) 60, 274, 1050, 1173, 1385
BsuTUI ATCGAT 1 cut(s) 1254
BtgI CCRYGG 1 cut(s) 897
BtrI CACGTC 1 cut(s) 1527
BtsCI GGATG 6 cut(s) 535, 561, 650, 986, 1086, 1116
BtsI GCAGTG 2 cut(s) 423, 468
BtsIMutI CAGTG 5 cut(s) 259, 423, 468, 1359, 1510
BveI ACCTGC 1 cut(s) 329
Cac8I GCNNGC 2 cut(s) 213, 1445
CfoI GCGC 5 cut(s) 213, 215, 651, 1066, 1076
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 7 cut(s) 58, 272, 776, 1171, 1208, 1245, 1480
ClaI ATCGAT 1 cut(s) 1254
Csp6I GTAC 4 cut(s) 344, 628, 948, 1450
CspCI CAANNNNNGTGG 2 cut(s) 1335, 1370
CviAII CATG 8 cut(s) 142, 613, 667, 820, 898, 1057, 1081, 1249
CviQI GTAC 4 cut(s) 344, 628, 948, 1450
DdeI CTNAG 3 cut(s) 677, 962, 1045
DpnI GATC 8 cut(s) 188, 939, 1020, 1253, 1257, 1266, 1282, 1359
DpnII GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
DraIII CACNNNGTG 1 cut(s) 915
DrdI GACNNNNNNGTC 1 cut(s) 1369
DseDI GACNNNNNNGTC 1 cut(s) 1369
Eam1104I CTCTTC 3 cut(s) 1002, 1294, 1512
EarI CTCTTC 3 cut(s) 1002, 1294, 1512
Eco130I CCWWGG 4 cut(s) 283, 576, 897, 1386
Eco147I AGGCCT 1 cut(s) 1385
Eco24I GRGCYC 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 999
Eco47I GGWCC 4 cut(s) 776, 1208, 1245, 1480
Eco57I CTGAAG 1 cut(s) 485
Eco88I CYCGRG 2 cut(s) 721, 1377
Eco91I GGTNACC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 1044
EcoO109I RGGNCCY 2 cut(s) 58, 776
EcoO65I GGTNACC 1 cut(s) 1153
EcoRII CCWGG 2 cut(s) 887, 1097
EcoT14I CCWWGG 4 cut(s) 283, 576, 897, 1386
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 4 cut(s) 283, 576, 897, 1386
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 8 cut(s) 145, 616, 670, 823, 901, 1060, 1084, 1252
FalI AAGNNNNNCTT 2 cut(s) 1392, 1424
FaqI GGGAC 2 cut(s) 789, 1214
FatI CATG 8 cut(s) 141, 612, 666, 819, 897, 1056, 1080, 1248
FbaI TGATCA 2 cut(s) 186, 937
FblI GTMKAC 2 cut(s) 1131, 1373
Fnu4HI GCNGC 6 cut(s) 198, 414, 789, 954, 1200, 1299
FokI GGATG 6 cut(s) 542, 568, 657, 993, 1073, 1103
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 6 cut(s) 198, 414, 789, 954, 1200, 1299
FspBI CTAG 1 cut(s) 347
FspI TGCGCA 1 cut(s) 1065
GlaI GCGC 5 cut(s) 212, 214, 650, 1065, 1075
GluI GCNGC 6 cut(s) 198, 414, 789, 954, 1200, 1299
GsaI CCCAGC 1 cut(s) 400
GsuI CTGGAG 2 cut(s) 475, 824
HaeII RGCGCY 2 cut(s) 652, 1077
HaeIII GGCC 5 cut(s) 60, 274, 1050, 1173, 1385
HapII CCGG 4 cut(s) 270, 494, 641, 1243
HhaI GCGC 5 cut(s) 213, 215, 651, 1066, 1076
Hin1II CATG 8 cut(s) 145, 616, 670, 823, 901, 1060, 1084, 1252
Hin6I GCGC 5 cut(s) 211, 213, 649, 1064, 1074
HinP1I GCGC 5 cut(s) 211, 213, 649, 1064, 1074
HincII GTYRAC 2 cut(s) 408, 718
HindII GTYRAC 2 cut(s) 408, 718
HinfI GANTC 3 cut(s) 806, 1314, 1497
HpaII CCGG 4 cut(s) 270, 494, 641, 1243
HphI GGTGA 3 cut(s) 321, 632, 1147
Hpy166II GTNNAC 9 cut(s) 241, 344, 408, 623, 718, 1007, 1132, 1374, 1452
Hpy188III TCNNGA 2 cut(s) 454, 1379
Hpy8I GTNNAC 9 cut(s) 241, 344, 408, 623, 718, 1007, 1132, 1374, 1452
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 5 cut(s) 817, 1087, 1156, 1271, 1315
HpyCH4III ACNGT 2 cut(s) 627, 1125
HpyCH4IV ACGT 2 cut(s) 845, 1526
HpyCH4V TGCA 9 cut(s) 338, 380, 425, 449, 788, 865, 1202, 1298, 1346
HpyF10VI GCNNNNNNNGC 9 cut(s) 266, 377, 393, 422, 824, 876, 1199, 1453, 1499
HpyF3I CTNAG 3 cut(s) 677, 962, 1045
HpySE526I ACGT 2 cut(s) 845, 1526
Hsp92II CATG 8 cut(s) 145, 616, 670, 823, 901, 1060, 1084, 1252
HspAI GCGC 5 cut(s) 211, 213, 649, 1064, 1074
Ksp22I TGATCA 2 cut(s) 186, 937
Kzo9I GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
LmnI GCTCC 2 cut(s) 208, 1306
Lsp1109I GCAGC 5 cut(s) 184, 800, 965, 1186, 1310
LweI GCATC 5 cut(s) 335, 680, 800, 1095, 1333
MaeI CTAG 1 cut(s) 347
MaeII ACGT 2 cut(s) 845, 1526
MaeIII GTNAC 4 cut(s) 733, 1026, 1153, 1337
MalI GATC 8 cut(s) 188, 939, 1020, 1253, 1257, 1266, 1282, 1359
MboI GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
MboII GAAGA 7 cut(s) 770, 898, 989, 1281, 1339, 1397, 1529
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 1 cut(s) 1357
MhlI GDGCHC 2 cut(s) 50, 389
MluCI AATT 9 cut(s) 34, 64, 190, 483, 585, 687, 768, 855, 923
MlyI GAGTC 1 cut(s) 815
MmeI TCCRAC 4 cut(s) 33, 958, 1291, 1385
MroXI GAANNNNTTC 2 cut(s) 470, 1318
MseI TTAA 6 cut(s) 63, 74, 354, 771, 834, 926
MslI CAYNNNNRTG 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 1185
MspI CCGG 4 cut(s) 270, 494, 641, 1243
MspR9I CCNGG 4 cut(s) 271, 642, 889, 1099
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 2 cut(s) 449, 1195
MvaI CCWGG 2 cut(s) 889, 1099
MvnI CGCG 4 cut(s) 213, 707, 1151, 1485
MwoI GCNNNNNNNGC 9 cut(s) 266, 377, 393, 422, 824, 876, 1199, 1453, 1499
NciI CCSGG 2 cut(s) 271, 642
NcoI CCATGG 1 cut(s) 897
NdeII GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
NlaIII CATG 8 cut(s) 145, 616, 670, 823, 901, 1060, 1084, 1252
NlaIV GGNNCC 5 cut(s) 574, 695, 777, 920, 1359
NmeAIII GCCGAG 1 cut(s) 1246
NmuCI GTSAC 2 cut(s) 1026, 1153
NsbI TGCGCA 1 cut(s) 1065
NspV TTCGAA 1 cut(s) 1275
PaqCI CACCTGC 1 cut(s) 329
PauI GCGCGC 1 cut(s) 211
PceI AGGCCT 1 cut(s) 1385
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 2 cut(s) 449, 1195
PdmI GAANNNNTTC 2 cut(s) 470, 1318
PfeI GAWTC 2 cut(s) 1314, 1497
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 6 cut(s) 199, 415, 790, 955, 1201, 1300
PleI GAGTC 1 cut(s) 814
PpsI GAGTC 1 cut(s) 814
PpuMI RGGWCCY 1 cut(s) 776
PshBI ATTAAT 2 cut(s) 74, 926
PsiI TTATAA 1 cut(s) 525
Psp1406I AACGTT 1 cut(s) 845
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 2 cut(s) 887, 1097
PspEI GGTNACC 1 cut(s) 1153
PspFI CCCAGC 1 cut(s) 396
PspGI CCWGG 2 cut(s) 887, 1097
PspN4I GGNNCC 5 cut(s) 574, 695, 777, 920, 1359
PspPI GGNCC 7 cut(s) 58, 272, 776, 1171, 1208, 1245, 1480
PspPPI RGGWCCY 1 cut(s) 776
PsuI RGATCY 1 cut(s) 1357
PsyI GACNNNGTC 1 cut(s) 11
PteI GCGCGC 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 1185
RsaI GTAC 4 cut(s) 345, 629, 949, 1451
RsaNI GTAC 4 cut(s) 344, 628, 948, 1450
RseI CAYNNNNRTG 1 cut(s) 665
SaqAI TTAA 6 cut(s) 63, 74, 354, 771, 834, 926
SatI GCNGC 6 cut(s) 198, 414, 789, 954, 1200, 1299
Sau3AI GATC 8 cut(s) 186, 937, 1018, 1251, 1255, 1264, 1280, 1357
Sau96I GGNCC 7 cut(s) 58, 272, 776, 1171, 1208, 1245, 1480
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 1 cut(s) 815
ScrFI CCNGG 4 cut(s) 271, 642, 889, 1099
SduI GDGCHC 2 cut(s) 50, 389
SfaNI GCATC 5 cut(s) 335, 680, 800, 1095, 1333
SfuI TTCGAA 1 cut(s) 1275
SinI GGWCC 4 cut(s) 776, 1208, 1245, 1480
SmiMI CAYNNNNRTG 1 cut(s) 665
SpeI ACTAGT 1 cut(s) 346
Sse9I AATT 9 cut(s) 34, 64, 190, 483, 585, 687, 768, 855, 923
SseBI AGGCCT 1 cut(s) 1385
SsiI CCGC 5 cut(s) 364, 371, 414, 1151, 1483
SspMI CTAG 1 cut(s) 347
StuI AGGCCT 1 cut(s) 1385
StyD4I CCNGG 4 cut(s) 269, 640, 887, 1097
StyI CCWWGG 4 cut(s) 283, 576, 897, 1386
TaaI ACNGT 2 cut(s) 627, 1125
TaiI ACGT 2 cut(s) 848, 1529
TaqI TCGA 6 cut(s) 110, 1021, 1254, 1267, 1275, 1412
TaqII GACCGA 1 cut(s) 96
TasI AATT 9 cut(s) 34, 64, 190, 483, 585, 687, 768, 855, 923
TatI WGTACW 3 cut(s) 343, 627, 1449
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 2 cut(s) 1314, 1497
Tru1I TTAA 6 cut(s) 63, 74, 354, 771, 834, 926
Tru9I TTAA 6 cut(s) 63, 74, 354, 771, 834, 926
TscAI CASTG 5 cut(s) 259, 423, 468, 1359, 1510
TseFI GTSAC 2 cut(s) 1026, 1153
TseI GCWGC 5 cut(s) 197, 788, 953, 1199, 1298
Tsp45I GTSAC 2 cut(s) 1026, 1153
TspDTI ATGAA 5 cut(s) 234, 289, 649, 836, 1069
TspGWI ACGGA 3 cut(s) 15, 1045, 1416
TspRI CASTG 5 cut(s) 259, 423, 468, 1359, 1510
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 4 cut(s) 776, 1208, 1245, 1480
VspI ATTAAT 2 cut(s) 74, 926
XagI CCTNNNNNAGG 1 cut(s) 1044
XapI RAATTY 1 cut(s) 34
XmiI GTMKAC 2 cut(s) 1131, 1373
XmnI GAANNNNTTC 2 cut(s) 470, 1318
XspI CTAG 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.