Rorug02G0108300
ERF Family

Major Facilitator Superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013883
Physical Location & Seq
Reverse (-)
9239065 .. 9267785
28721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0108300.1

Sequence Viewer

Length: 2829 bp
ATGAGTGAACAATTCACCATGAATATAGCTTGGCTCCTGCTGTTGATGGTTTTCTTTAATGGGTTTGCCTCACATGGAGCTACCACCAACATCTCTACAAGACCAGAAGAAGTAAATGTTGGGGCTATGCTTGCTTTCAATTCCATTGTTGGCAAAGTTGCCAAAGTGGCAATACAAGCTGCTGTTGAAGATGTGAATTCTGACCCTTCTGTTCTCAATGGAACCAAGATGAAAGTTACAATGCAGAATTCAAATTACAGTGGATTTCTGGGCATAATTGAGGCTTTACGATTCATGGAGAACGACACAGTGGCCATAATTGGACCCCAGAATGCTGTAACGGCTCATGCAGTATCTCACATTGCAAATGAGCTGCACACTCCTCTGTTGTCATTTGCAGTAACAGATCCCACCCTGTCTTCACTTCAGTTCCCATATTTTGTTAGGACTACTCAGAACGATTTGTTTCAGATGGCTGCAATAGCCGATATGGTTGAGTATTATGGATGGAGAGAGGTAATTGCAATATATGTTGATGATGATTTAGGGAGAAATGGGATTGCAGCATTAGGGGATTTGCTTGCTGAGAAACGTTCTAAGATCTCATACAAAGCACCTCTGGTTCTTGATCCTACTCGGAATAACATCACTGATGTACTGATTAAAGTGTCTTTAACTGAGTCTAGGATTATCGTTCTTCATGTTTATCCAGGTTGGGGCCCTGAGGTGCTTAGTGTGGCCAATTATCTTGGCATGATGGGAACAGGGTTTGTTTGGATAGCTACTCATTGGCTTTCTAGTTATATAGATTCCACCATTCCCCTCCCTATGAGTACTTTGGATAATATGCAAGGAGTTCTAACATTGCGCATGTACACGCCAGATACAGAACAGAAAAGAAAATTTGTTTCGAGGTGGAGCAACTTGACTAGTGGTAACCAAATTGGGTTGAATGCTTTCGGTCTCTATGCTTATGACTCTGTATGGTTGCTTGCTCATGCACTTGATGCCTTTTTTGATCAAGGGGGAAACATTTCATTCTCAAATGATTCAAGGTTGACTCAGATGCCCAGAGGGGACCTAAATCTTGAATATCTGAATATATTTGATGGAGGGAGCTTGTTACTTCGTAACATATTTGGAGTTAATATGACTGGCACAACTGGCTCGTTTAAGTATACTCCAGATAGGGACCTCATTCGTCCTGCTTATGAAATCATAAATGTGATTGGCACTGGGGTTAGGAGGATTGGTTATTGGTCTAACCATTCTGGTTTATCAGTTAAGCCTCCAGAAACATTTTACACAACCCCACCAAATCGTTCTAGTCCGAACCAAAGCCTTTACAGTGTGATTTGGCCTGGAGAGACGGCACAGAAGCCTCGTGGTTGGGTTTTCCCAAACAATGGAAGGCATTTGAAGATTGCAGTCCCAAACCGTGCTAGTTTCCGTGAATTTGTATCATATACACGAAGCAATGACATGTTCACTGGGTACTGCATTGATGTTTTTACTGCTGCATTGAACTTGTTGCCCTATGCTGTTCCCTACAAACTTTTTCATATTGGTGATGGTAAAAGAAATCCAAGGAGCACTGATCTTGTGCACACAATCCAAACGGGTGAATATGATGCAGTAGTAGGTGATATTGCAATCACCACCAACAGAACCAGGATGGCAGATTTTACGCAGCCATACATCGAATCTGGGCTAGTAGTAGTTGCACCTGTTAAGACGACATTGAATTCAAATCCTTGGGCATTTCTGAGGCCATTCAATAAAATGATGTGGGCTGTCACTGCTGCCTTCTTTCTCATTGTTGGAACAGTTGTTTGGATATTAGAGCATCGACTGAATGATGAATTTCGGGGCCCTCCTAGAAGACAACTCGTCACTATTCTTTGGTTTAGCTTTTCAACTTGGTTCTTTGCCCATAGAGAAAATACAGTCAGCACCCTGGGTCGCCTAGTACTAATCATATGGCTATTTGTGGTTCTTATCATCAACTCAAGCTATACTGCAAGTTTGACCTCAATCCTAACGGTGCAAAAGCTTTCTTCCTCCATCAAAGGCATTGAAACTTTGCTCGAAAGCAAGGACCCCATTGGTTACCAACAGGGTTCATTTGCCGAGTATTATCTCATTGAGGAACTCCACATTGACAGTTCCAGACTCGTGCCACTCGTCATGCCAGAAGATTATGTGAGAGCCTTAAAAGCCGGTCCACATAAAGAAGGTGGTGTTGCCGCAGTTATTGATGAGCGTGCATACATGGAGCTATTCCTCTCAAGTCGATGCGATTTCAGCATTGTAGGGCAAGAATTTACCAAAACCGGATGGGGATTTGCTTTTGCAAGGGACTCGCCTCTATCAGTGGACCTGTCAACTGCTCTGTTGAAGCTGTCGGAGAATGGGGATCTACAGAGGATCCATGACAAATGGCTTCTCAGAAGTCCTTGTACTTTACAAGGAGCAAAGCTAGAAGTGGACAGGCTTGAACTTAGAAGCTTCTCGGCCCTTTTTATTATCTGTGGCGGAGCTTGTTTGGTTGCTCTCATTATATATTTCAGCATGATGTGTTACCAGTTCACCAAGCGGTACACTGACAGTTTGTCTTCTTCCGGGAGCTCCACATCTCGGCGTCTTCAGACCTTTCTCACATTTGTTGACGAGAAGGAAGAAGTACAAAGCCGATCCAAAAGGAGAAGCATGGAGAAGATGTCAAACCGAAGTGTGGGTGAAGATGACTCGTCAAACAGTTCCAAAAGAAGACATATAGATCAGTACTCATGTGCAAGCAGAAGTTTTGAAGATAGTAACAATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0002165 GO:0003006 GO:0003376 GO:0003674 GO:0005215 GO:0005319 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005770 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0006897 GO:0006915 GO:0006928 GO:0006950 GO:0006996 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007399 GO:0007416 GO:0007417 GO:0007528 GO:0007610 GO:0007617 GO:0007618 GO:0007619 GO:0008150 GO:0008219 GO:0008333 GO:0008347 GO:0008582 GO:0009267 GO:0009605 GO:0009653 GO:0009791 GO:0009886 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010001 GO:0010008 GO:0010623 GO:0010646 GO:0010648 GO:0010876 GO:0010941 GO:0012501 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016477 GO:0019098 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023057 GO:0030154 GO:0031090 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031902 GO:0031982 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033554 GO:0035193 GO:0036465 GO:0040007 GO:0040008 GO:0040011 GO:0042063 GO:0042594 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045476 GO:0045477 GO:0045595 GO:0045924 GO:0046624 GO:0046907 GO:0048468 GO:0048477 GO:0048488 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0048609 GO:0048638 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050803 GO:0050807 GO:0050808 GO:0050896 GO:0051124 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051641 GO:0051649 GO:0051674 GO:0051704 GO:0051716 GO:0051960 GO:0051963 GO:0060180 GO:0060284 GO:0065007 GO:0065008 GO:0071496 GO:0071702 GO:0071840 GO:0080171 GO:0090092 GO:0090097 GO:0090099 GO:0090101 GO:0090520 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0099003 GO:0099504 GO:1902742 GO:1904396 GO:1904748 GO:1905879 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1

Protein Analysis

942

Amino Acids

105.49

Weight (kDa)

6.65

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 48 - 337 6.4e-19 Periplasmic binding protein
ANF_receptor PF01094 55 - 411 3.5e-88 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 490 - 576 3.9e-08 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 529 - 815 1.8e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 595 - 848 4.3e-34 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36780
fragaria_vesca FvH4_1g14040
malus_domestica MD15G1267600.v1.1 MD15G1267800.v1.1
prunus_persica Prupe.7G149000_v2.0.a1
pyrus_communis pycom02g12160 pycom15g23310
rosa_chinensis RchiOBHm_Chr2g0102631
rosa_laevigata RLG00000017158
rosa_multiflora Rmu_sc0003852.1_g000007
rosa_roxburghii Rroxscaffold_2G00140430
rosa_rugosa Rorug02G0108300
rosa_samantha Rh2AG157000 Rh2BG163300 Rh2CG162400 Rh2DG162200
rosa_wichuraiana Rw2G012180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 1406
AccI GTMKAC 1 cut(s) 1178
AciI CCGC 3 cut(s) 2247, 2535, 2596
AclI AACGTT 1 cut(s) 592
AclWI GGATC 6 cut(s) 401, 623, 2422, 2424, 2435, 2688
AcoI YGGCCR 2 cut(s) 312, 738
AcsI RAATTY 7 cut(s) 196, 247, 902, 1454, 1744, 1862, 2321
AcuI CTGAAG 2 cut(s) 410, 2630
AcyI GRCGYC 1 cut(s) 2641
AfaI GTAC 9 cut(s) 657, 835, 875, 1496, 1971, 2461, 2600, 2685, 2786
AfiI CCNNNNNNNGG 4 cut(s) 716, 1406, 2637, 2734
AflIII ACRYGT 1 cut(s) 1482
AhdI GACNNNNNGTC 2 cut(s) 1889, 2611
AhlI ACTAGT 1 cut(s) 929
AjnI CCWGG 4 cut(s) 709, 1360, 1670, 1956
AjuI GAANNNNNNNTTGG 4 cut(s) 102, 134, 1816, 1848
Alw21I GWGCWC 3 cut(s) 1595, 1608, 2630
Alw26I GTCTC 2 cut(s) 968, 1361
Alw44I GTGCAC 1 cut(s) 1604
AlwI GGATC 6 cut(s) 401, 623, 2422, 2424, 2435, 2688
AoxI GGCC 7 cut(s) 312, 718, 738, 1358, 1769, 1870, 2514
ApaI GGGCCC 2 cut(s) 722, 1874
ApaLI GTGCAC 1 cut(s) 1604
ApeKI GCWGC 7 cut(s) 179, 373, 476, 563, 1517, 1690, 1802
ApoI RAATTY 7 cut(s) 196, 247, 902, 1454, 1744, 1862, 2321
Asp700I GAANNNNTTC 2 cut(s) 956, 1033
AspLEI GCGC 1 cut(s) 870
AsuC2I CCSGG 1 cut(s) 2623
AsuHPI GGTGA 7 cut(s) 7, 1580, 1634, 1648, 1655, 2581, 2750
AvaII GGWCC 6 cut(s) 323, 1078, 1192, 2098, 2222, 2377
AxyI CCTNAGG 1 cut(s) 723
BaeGI GKGCMC 3 cut(s) 722, 1608, 1874
BalI TGGCCA 2 cut(s) 314, 740
BamHI GGATCC 1 cut(s) 2427
BanII GRGCYC 3 cut(s) 722, 1874, 2630
BarI GAAGNNNNNNTAC 2 cut(s) 2443, 2475
BauI CACGAG 2 cut(s) 1383, 2174
BbsI GAAGAC 5 cut(s) 411, 1888, 2607, 2636, 2776
Bbv12I GWGCWC 3 cut(s) 1595, 1608, 2630
BbvI GCAGC 7 cut(s) 166, 360, 463, 575, 1504, 1702, 1789
BccI CCATC 9 cut(s) 40, 466, 501, 751, 1103, 1565, 1669, 2072, 2331
BceAI ACGGC 2 cut(s) 357, 1386
BcgI CGANNNNNNTGC 2 cut(s) 2343, 2377
BciT130I CCWGG 4 cut(s) 711, 1362, 1672, 1958
BclI TGATCA 1 cut(s) 1018
BcnI CCSGG 1 cut(s) 2623
BcoDI GTCTC 2 cut(s) 968, 1361
BcuI ACTAGT 1 cut(s) 929
BfaI CTAG 9 cut(s) 684, 798, 930, 1326, 1443, 1712, 1878, 1967, 2480
BfmI CTRYAG 1 cut(s) 2420
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 600
BisI GCNGC 8 cut(s) 180, 374, 477, 564, 1518, 1691, 1803, 2247
BlsI GCNGC 8 cut(s) 181, 375, 478, 565, 1519, 1692, 1804, 2248
BmcAI AGTACT 3 cut(s) 835, 1971, 2786
Bme1390I CCNGG 5 cut(s) 711, 1362, 1672, 1958, 2623
Bme18I GGWCC 6 cut(s) 323, 1078, 1192, 2098, 2222, 2377
BmeRI GACNNNNNGTC 2 cut(s) 1889, 2611
BmrFI CCNGG 5 cut(s) 711, 1362, 1672, 1958, 2623
BmrI ACTGGG 2 cut(s) 1245, 1500
BmsI GCATC 5 cut(s) 997, 1056, 1621, 1855, 2285
BmuI ACTGGG 2 cut(s) 1245, 1500
BpiI GAAGAC 5 cut(s) 411, 1888, 2607, 2636, 2776
BpmI CTGGAG 3 cut(s) 1167, 1275, 1383
BpuEI CTTGAG 2 cut(s) 1993, 2272
BpuMI CCSGG 1 cut(s) 2623
BsaHI GRCGYC 1 cut(s) 2641
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 4 cut(s) 1586, 1754, 1956, 1957
BsaWI WCCGGW 1 cut(s) 2333
BsaXI ACNNNNNCTCC 4 cut(s) 1237, 1267, 2705, 2735
Bsc4I CCNNNNNNNGG 4 cut(s) 716, 1406, 2637, 2734
Bse118I RCCGGY 1 cut(s) 2219
Bse1I ACTGG 5 cut(s) 1159, 1168, 1240, 1495, 2584
Bse21I CCTNAGG 1 cut(s) 723
Bse3DI GCAATG 3 cut(s) 360, 863, 1483
BseBI CCWGG 4 cut(s) 711, 1362, 1672, 1958
BseDI CCNNGG 4 cut(s) 1586, 1754, 1956, 1957
BseGI GGATG 3 cut(s) 512, 1680, 2342
BseLI CCNNNNNNNGG 4 cut(s) 716, 1406, 2637, 2734
BseMI GCAATG 3 cut(s) 360, 863, 1483
BseMII CTCAG 7 cut(s) 467, 576, 669, 714, 1076, 1757, 2461
BseNI ACTGG 5 cut(s) 1159, 1168, 1240, 1495, 2584
BseRI GAGGAG 1 cut(s) 372
BseSI GKGCMC 3 cut(s) 722, 1608, 1874
BseXI GCAGC 7 cut(s) 166, 360, 463, 575, 1504, 1702, 1789
BsgI GTGCAG 1 cut(s) 359
BshFI GGCC 7 cut(s) 314, 720, 740, 1360, 1771, 1872, 2516
BsiHKAI GWGCWC 3 cut(s) 1595, 1608, 2630
BsiSI CCGG 3 cut(s) 2220, 2334, 2622
BslFI GGGAC 4 cut(s) 1091, 1205, 1415, 2372
BslI CCNNNNNNNGG 4 cut(s) 716, 1406, 2637, 2734
BsmAI GTCTC 2 cut(s) 968, 1361
BsmBI CGTCTC 1 cut(s) 1361
BsmFI GGGAC 4 cut(s) 1091, 1205, 1415, 2372
BsmI GAATGC 2 cut(s) 337, 958
BsnI GGCC 7 cut(s) 314, 720, 740, 1360, 1771, 1872, 2516
Bso31I GGTCTC 1 cut(s) 968
Bsp120I GGGCCC 2 cut(s) 718, 1870
Bsp1286I GDGCHC 5 cut(s) 722, 1595, 1608, 1874, 2630
Bsp1407I TGTACA 1 cut(s) 873
Bsp143I GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
BspACI CCGC 3 cut(s) 2247, 2535, 2596
BspANI GGCC 7 cut(s) 314, 720, 740, 1360, 1771, 1872, 2516
BspCNI CTCAG 7 cut(s) 466, 577, 670, 715, 1075, 1758, 2460
BspPI GGATC 6 cut(s) 401, 623, 2422, 2424, 2435, 2688
BspTNI GGTCTC 1 cut(s) 968
BsrDI GCAATG 3 cut(s) 360, 863, 1483
BsrFI RCCGGY 1 cut(s) 2219
BsrGI TGTACA 1 cut(s) 873
BsrI ACTGG 5 cut(s) 1159, 1168, 1240, 1495, 2584
BssAI RCCGGY 1 cut(s) 2219
BssECI CCNNGG 4 cut(s) 1586, 1754, 1956, 1957
BssMI GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
BssNAI GTATAC 1 cut(s) 1179
BssNI GRCGYC 1 cut(s) 2641
BssSI CACGAG 2 cut(s) 1383, 2174
BssT1I CCWWGG 2 cut(s) 1586, 1754
Bst1107I GTATAC 1 cut(s) 1179
Bst2BI CACGAG 2 cut(s) 1383, 2174
Bst2UI CCWGG 4 cut(s) 711, 1362, 1672, 1958
BstACI GRCGYC 1 cut(s) 2641
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 873
BstC8I GCNNGC 5 cut(s) 132, 582, 993, 2265, 2797
BstEII GGTNACC 2 cut(s) 935, 2108
BstF5I GGATG 3 cut(s) 512, 1680, 2342
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 9 cut(s) 409, 603, 631, 1021, 1600, 2419, 2430, 2696, 2782
BstMAI GTCTC 2 cut(s) 968, 1361
BstMBI GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
BstNI CCWGG 4 cut(s) 711, 1362, 1672, 1958
BstNSI RCATGY 2 cut(s) 874, 1486
BstPI GGTNACC 2 cut(s) 935, 2108
BstSCI CCNGG 5 cut(s) 709, 1360, 1670, 1956, 2621
BstSFI CTRYAG 1 cut(s) 2420
BstSLI GKGCMC 3 cut(s) 722, 1608, 1874
BstV1I GCAGC 7 cut(s) 166, 360, 463, 575, 1504, 1702, 1789
BstV2I GAAGAC 5 cut(s) 411, 1888, 2607, 2636, 2776
BstX2I RGATCY 4 cut(s) 406, 600, 2416, 2427
BstYI RGATCY 4 cut(s) 406, 600, 2416, 2427
BstZ17I GTATAC 1 cut(s) 1179
Bsu36I CCTNAGG 1 cut(s) 723
BsuRI GGCC 7 cut(s) 314, 720, 740, 1360, 1771, 1872, 2516
BtsCI GGATG 3 cut(s) 512, 1680, 2342
BtsI GCAGTG 1 cut(s) 1797
Cac8I GCNNGC 5 cut(s) 132, 582, 993, 2265, 2797
CfoI GCGC 1 cut(s) 870
Cfr10I RCCGGY 1 cut(s) 2219
CseI GACGC 1 cut(s) 2630
Csp6I GTAC 9 cut(s) 656, 834, 874, 1495, 1970, 2460, 2599, 2684, 2785
CviQI GTAC 9 cut(s) 656, 834, 874, 1495, 1970, 2460, 2599, 2684, 2785
DpnI GATC 9 cut(s) 408, 602, 630, 1020, 1599, 2418, 2429, 2695, 2781
DpnII GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
DriI GACNNNNNGTC 2 cut(s) 1889, 2611
EaeI YGGCCR 2 cut(s) 312, 738
Eam1105I GACNNNNNGTC 2 cut(s) 1889, 2611
EciI GGCGGA 1 cut(s) 2550
Ecl136II GAGCTC 1 cut(s) 2628
Eco130I CCWWGG 2 cut(s) 1586, 1754
Eco24I GRGCYC 3 cut(s) 722, 1874, 2630
Eco31I GGTCTC 1 cut(s) 968
Eco47I GGWCC 6 cut(s) 323, 1078, 1192, 2098, 2222, 2377
Eco53kI GAGCTC 1 cut(s) 2628
Eco57I CTGAAG 2 cut(s) 410, 2630
Eco81I CCTNAGG 1 cut(s) 723
Eco91I GGTNACC 2 cut(s) 935, 2108
EcoICRI GAGCTC 1 cut(s) 2628
EcoO109I RGGNCCY 7 cut(s) 718, 719, 1078, 1192, 1870, 1871, 2098
EcoO65I GGTNACC 2 cut(s) 935, 2108
EcoRI GAATTC 3 cut(s) 196, 247, 1744
EcoRII CCWGG 4 cut(s) 709, 1360, 1670, 1956
EcoT14I CCWWGG 2 cut(s) 1586, 1754
EcoT38I GRGCYC 3 cut(s) 722, 1874, 2630
ErhI CCWWGG 2 cut(s) 1586, 1754
Esp3I CGTCTC 1 cut(s) 1361
FaqI GGGAC 4 cut(s) 1091, 1205, 1415, 2372
FauNDI CATATG 1 cut(s) 1979
FbaI TGATCA 1 cut(s) 1018
FblI GTMKAC 1 cut(s) 1178
Fnu4HI GCNGC 8 cut(s) 180, 374, 477, 564, 1518, 1691, 1803, 2247
FokI GGATG 3 cut(s) 519, 1687, 2349
FriOI GRGCYC 3 cut(s) 722, 1874, 2630
Fsp4HI GCNGC 8 cut(s) 180, 374, 477, 564, 1518, 1691, 1803, 2247
FspBI CTAG 9 cut(s) 684, 798, 930, 1326, 1443, 1712, 1878, 1967, 2480
FspI TGCGCA 1 cut(s) 869
GlaI GCGC 1 cut(s) 869
GluI GCNGC 8 cut(s) 180, 374, 477, 564, 1518, 1691, 1803, 2247
GsuI CTGGAG 3 cut(s) 1167, 1275, 1383
HaeIII GGCC 7 cut(s) 314, 720, 740, 1360, 1771, 1872, 2516
HapII CCGG 3 cut(s) 2220, 2334, 2622
HgaI GACGC 1 cut(s) 2630
HhaI GCGC 1 cut(s) 870
Hin1I GRCGYC 1 cut(s) 2641
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HincII GTYRAC 3 cut(s) 1059, 2385, 2668
HindII GTYRAC 3 cut(s) 1059, 2385, 2668
HindIII AAGCTT 2 cut(s) 2051, 2506
HpaII CCGG 3 cut(s) 2220, 2334, 2622
HphI GGTGA 7 cut(s) 7, 1580, 1634, 1648, 1655, 2581, 2750
Hpy188III TCNNGA 5 cut(s) 626, 1088, 1184, 1292, 2169
HpyAV CCTTC 5 cut(s) 216, 1404, 1816, 2228, 2668
HpyCH4IV ACGT 1 cut(s) 592
HpySE526I ACGT 1 cut(s) 592
Hsp92I GRCGYC 1 cut(s) 2641
HspAI GCGC 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 1018
Kzo9I GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
Lsp1109I GCAGC 7 cut(s) 166, 360, 463, 575, 1504, 1702, 1789
LweI GCATC 5 cut(s) 997, 1056, 1621, 1855, 2285
MaeI CTAG 9 cut(s) 684, 798, 930, 1326, 1443, 1712, 1878, 1967, 2480
MaeII ACGT 1 cut(s) 592
MalI GATC 9 cut(s) 408, 602, 630, 1020, 1599, 2418, 2429, 2695, 2781
MboI GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
MflI RGATCY 4 cut(s) 406, 600, 2416, 2427
MhlI GDGCHC 5 cut(s) 722, 1595, 1608, 1874, 2630
MlsI TGGCCA 2 cut(s) 314, 740
MluNI TGGCCA 2 cut(s) 314, 740
MlyI GAGTC 6 cut(s) 689, 971, 1054, 2166, 2354, 2741
MmeI TCCRAC 2 cut(s) 1801, 2385
Mox20I TGGCCA 2 cut(s) 314, 740
MroXI GAANNNNTTC 2 cut(s) 956, 1033
MscI TGGCCA 2 cut(s) 314, 740
MseI TTAA 8 cut(s) 57, 663, 674, 1146, 1173, 1284, 1731, 2213
MslI CAYNNNNRTG 2 cut(s) 1223, 1566
Msp20I TGGCCA 2 cut(s) 314, 740
MspI CCGG 3 cut(s) 2220, 2334, 2622
MspR9I CCNGG 5 cut(s) 711, 1362, 1672, 1958, 2623
Mva1269I GAATGC 2 cut(s) 337, 958
MvaI CCWGG 4 cut(s) 711, 1362, 1672, 1958
NciI CCSGG 1 cut(s) 2623
NdeI CATATG 1 cut(s) 1979
NdeII GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
NmeAIII GCCGAG 3 cut(s) 2155, 2492, 2617
NmuCI GTSAC 2 cut(s) 1795, 1891
NsbI TGCGCA 1 cut(s) 869
NspI RCATGY 2 cut(s) 874, 1486
PasI CCCWGGG 1 cut(s) 1957
PciI ACATGT 1 cut(s) 1482
PcsI WCGNNNNNNNCGW 1 cut(s) 2181
PctI GAATGC 2 cut(s) 337, 958
PdmI GAANNNNTTC 2 cut(s) 956, 1033
PfeI GAWTC 4 cut(s) 291, 809, 1049, 1703
PflMI CCANNNNNTGG 1 cut(s) 1406
PfoI TCCNGGA 1 cut(s) 2621
PkrI GCNGC 8 cut(s) 181, 375, 478, 565, 1519, 1692, 1804, 2248
PleI GAGTC 6 cut(s) 688, 971, 1054, 2166, 2354, 2741
PpsI GAGTC 6 cut(s) 688, 971, 1054, 2166, 2354, 2741
PpuMI RGGWCCY 3 cut(s) 1078, 1192, 2098
PscI ACATGT 1 cut(s) 1482
Psp124BI GAGCTC 1 cut(s) 2630
Psp1406I AACGTT 1 cut(s) 592
Psp5II RGGWCCY 3 cut(s) 1078, 1192, 2098
Psp6I CCWGG 4 cut(s) 709, 1360, 1670, 1956
PspEI GGTNACC 2 cut(s) 935, 2108
PspGI CCWGG 4 cut(s) 709, 1360, 1670, 1956
PspOMI GGGCCC 2 cut(s) 718, 1870
PspPPI RGGWCCY 3 cut(s) 1078, 1192, 2098
PsuI RGATCY 4 cut(s) 406, 600, 2416, 2427
RsaI GTAC 9 cut(s) 657, 835, 875, 1496, 1971, 2461, 2600, 2685, 2786
RsaNI GTAC 9 cut(s) 656, 834, 874, 1495, 1970, 2460, 2599, 2684, 2785
RseI CAYNNNNRTG 2 cut(s) 1223, 1566
SacI GAGCTC 1 cut(s) 2630
SaqAI TTAA 8 cut(s) 57, 663, 674, 1146, 1173, 1284, 1731, 2213
SatI GCNGC 8 cut(s) 180, 374, 477, 564, 1518, 1691, 1803, 2247
Sau3AI GATC 9 cut(s) 406, 600, 628, 1018, 1597, 2416, 2427, 2693, 2779
ScaI AGTACT 3 cut(s) 835, 1971, 2786
SchI GAGTC 6 cut(s) 689, 971, 1054, 2166, 2354, 2741
ScrFI CCNGG 5 cut(s) 711, 1362, 1672, 1958, 2623
SduI GDGCHC 5 cut(s) 722, 1595, 1608, 1874, 2630
SfaNI GCATC 5 cut(s) 997, 1056, 1621, 1855, 2285
SfcI CTRYAG 1 cut(s) 2420
SinI GGWCC 6 cut(s) 323, 1078, 1192, 2098, 2222, 2377
SmiMI CAYNNNNRTG 2 cut(s) 1223, 1566
SmlI CTYRAG 2 cut(s) 2008, 2287
SmoI CTYRAG 2 cut(s) 2008, 2287
SpeI ACTAGT 1 cut(s) 929
SsiI CCGC 3 cut(s) 2247, 2535, 2596
SspMI CTAG 9 cut(s) 684, 798, 930, 1326, 1443, 1712, 1878, 1967, 2480
SstI GAGCTC 1 cut(s) 2630
StyD4I CCNGG 5 cut(s) 709, 1360, 1670, 1956, 2621
StyI CCWWGG 2 cut(s) 1586, 1754
TaiI ACGT 1 cut(s) 595
TaqI TCGA 5 cut(s) 911, 1701, 1849, 2088, 2293
TaqII GACCGA 1 cut(s) 950
TatI WGTACW 7 cut(s) 655, 833, 873, 1969, 2459, 2683, 2784
TauI GCSGC 1 cut(s) 2249
TfiI GAWTC 4 cut(s) 291, 809, 1049, 1703
Tru1I TTAA 8 cut(s) 57, 663, 674, 1146, 1173, 1284, 1731, 2213
Tru9I TTAA 8 cut(s) 57, 663, 674, 1146, 1173, 1284, 1731, 2213
TseFI GTSAC 2 cut(s) 1795, 1891
TseI GCWGC 7 cut(s) 179, 373, 476, 563, 1517, 1690, 1802
Tsp45I GTSAC 2 cut(s) 1795, 1891
TspDTI ATGAA 9 cut(s) 35, 245, 283, 689, 1026, 1227, 1550, 1875, 2112
TspGWI ACGGA 1 cut(s) 1439
Van91I CCANNNNNTGG 1 cut(s) 1406
VneI GTGCAC 1 cut(s) 1604
VpaK11BI GGWCC 6 cut(s) 323, 1078, 1192, 2098, 2222, 2377
XapI RAATTY 7 cut(s) 196, 247, 902, 1454, 1744, 1862, 2321
XceI RCATGY 2 cut(s) 874, 1486
XmiI GTMKAC 1 cut(s) 1178
XmnI GAANNNNTTC 2 cut(s) 956, 1033
XspI CTAG 9 cut(s) 684, 798, 930, 1326, 1443, 1712, 1878, 1967, 2480
ZrmI AGTACT 3 cut(s) 835, 1971, 2786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.