Rh2AG157100
ERF Family

Major Facilitator Superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014169
Physical Location & Seq
Forward (+)
14757703 .. 14761325
3623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG157100.1

Sequence Viewer

Length: 1497 bp
ATGGGCCTTAAACGGGCCTCCAGATACCCATCTTCCTCTTTTCTATCAACACCCGGTTTTTTTAGACGTTTGGAAGCCATGAGAACAAGAAAGGTTTTGGGGGTTTCTCTGTCTCTCATTCTCATCAACTTGGCTGCTATAATGGAGCGAGCCGATGAGAATCTCCTCCCATCTGTGTACAAAGAAGTCAGTGAAGCTTTCAATGCAGGGCCATCTGACCTCGGATATCTCACATTCATCAGGAACTTTGTGCAGGGAATGGCATCTCCCCTTGCAGGTGTACTAGTCCTCTTATACGACCGCCCCACTGTTCTTGCAATGGGCACTTTCTGCTGGGCAATATCAACTGCTGCAGTGGGTGCAAGCCACCATTTTGCTCAAGCTGCACTCTGGAGAGCAGTGAATGGGTTCGGGCTGGCAATTGTCATACCAGCACTTCAGTCTTTCATTGCTGATAGCTATAAGGACAGCGTGAGAGGGGCCGGGTTTGGGATGGTAAGTCTTGTTGGTTCTTTGGGTGGCATTGGAGGCGGTGTTGTGGCCACGCTTATGGCTGGTAACATGTACTGGAGCGTACCTGGATGGCGTATTGCCTTCATTCTAATGGCAGCACTGAGTTCGCTAATTGGGTTCCTGGTGTTCATGTTTGTGGTTGACCCTAGAAAATTAGTTCCTATCACTCATGGTGCTGACCAGAGTTTTGAGAGGGATGATTTGATAATAAAGGGCAGTACTACTTCAACTGCTGCATCAGTTTGGATCGAGTCTTGGACAGCCATGAAGTCGGTTGTTAAAGTGCAAACATTTCAAATAATTGTCTTGCAGGGTGTTGTTGGGTCACTACCATGGACTGCCATGGTGTTCTTCACTATGTGGTTCGAACTTATAGGTTTTGATCACAACAGTACAGCGGCCCTCCTCAGTCTCTTTGCTATTGGATGTGCTACGGGTTCTCTTCTTGGTGGAATAATAGCTGATCGAATGTCACGAATCTACCCCCACACTGGCCGCATCATGTGTGCGCAGTTCAGCGCCTTCATGGGAATTCCATTTTCTTGGTTTTTACTCAAAGTAATCCCGCAATCAGTAGACAGCTATTACACATTTGCCGTCACTCTCACCTTGATGGGTTTAACTATCAGCTGGAATGCTACTGCTGCAAATGGTCCTATGTTTGCTGAGGTTGTTCCTACCAAACACCGAACTATGATTTATGCATTTGATCGGGCTTTCGAAGGATCCTTCTCTTCTTTTGCTGCTCCTTTGGTTGGAATTCTTTCAGAGAAGATGTTTGGCTATGATGCAAAATCTGTGGATCCGATTAAAGGGTCTACTAGGGAGGCCTTTGCATTATCTCAAGGGCTTATTTCGATGATGGCAGTTCCATTTGGTTTGTGTTGCTTGTTTTATACGCCTTTGTATGTGATTTTCAGAAAGGACCTTGAGAGTGCTAGAATTGCCAGTGCAAAAGAGGAAGAGATGAGCCTAACGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0002165 GO:0003006 GO:0003376 GO:0003674 GO:0005215 GO:0005319 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005770 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0006897 GO:0006915 GO:0006928 GO:0006950 GO:0006996 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007399 GO:0007416 GO:0007417 GO:0007528 GO:0007610 GO:0007617 GO:0007618 GO:0007619 GO:0008150 GO:0008219 GO:0008333 GO:0008347 GO:0008582 GO:0009267 GO:0009605 GO:0009653 GO:0009791 GO:0009886 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010001 GO:0010008 GO:0010623 GO:0010646 GO:0010648 GO:0010876 GO:0010941 GO:0012501 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016477 GO:0019098 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023057 GO:0030154 GO:0031090 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031902 GO:0031982 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033554 GO:0035193 GO:0036465 GO:0040007 GO:0040008 GO:0040011 GO:0042063 GO:0042594 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045476 GO:0045477 GO:0045595 GO:0045924 GO:0046624 GO:0046907 GO:0048468 GO:0048477 GO:0048488 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0048609 GO:0048638 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050803 GO:0050807 GO:0050808 GO:0050896 GO:0051124 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051641 GO:0051649 GO:0051674 GO:0051704 GO:0051716 GO:0051960 GO:0051963 GO:0060180 GO:0060284 GO:0065007 GO:0065008 GO:0071496 GO:0071702 GO:0071840 GO:0080171 GO:0090092 GO:0090097 GO:0090099 GO:0090101 GO:0090520 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0099003 GO:0099504 GO:1902742 GO:1904396 GO:1904748 GO:1905879 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1

Protein Analysis

498

Amino Acids

54.04

Weight (kDa)

8.98

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 44 - 355 6.4e-30 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36790
fragaria_vesca FvH4_1g14050 FvH4_1g14050
malus_domestica MD02G1154100.v1.1 MD15G1268500.v1.1
prunus_persica Prupe.7G148900_v2.0.a1
pyrus_communis pycom02g12170
rosa_chinensis RchiOBHm_Chr2g0102651
rosa_laevigata RLG00000017159
rosa_multiflora Rmu_sc0003852.1_g000017
rosa_roxburghii Rroxscaffold_2G00140420
rosa_rugosa Rorug02G0108300
rosa_samantha Rh2AG157100 Rh2BG163400 Rh2CG162600 Rh2DG162300
rosa_wichuraiana Rw2G012190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc16I TGCGCA 1 cut(s) 1023
Acc36I ACCTGC 1 cut(s) 266
AccI GTMKAC 2 cut(s) 1089, 1331
AciI CCGC 5 cut(s) 301, 531, 911, 1009, 1079
AclWI GGATC 5 cut(s) 767, 1233, 1246, 1310, 1323
AcoI YGGCCR 2 cut(s) 540, 1006
AcsI RAATTY 2 cut(s) 1044, 1272
AcuI CTGAAG 1 cut(s) 422
AdeI CACNNNGTG 1 cut(s) 873
AfaI GTAC 6 cut(s) 179, 282, 566, 576, 733, 907
AfiI CCNNNNNNNGG 6 cut(s) 13, 275, 489, 1004, 1268, 1325
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 3 cut(s) 202, 741, 809
AhlI ACTAGT 1 cut(s) 283
AjnI CCWGG 2 cut(s) 577, 633
AluBI AGCT 6 cut(s) 197, 383, 459, 974, 1095, 1143
AluI AGCT 6 cut(s) 197, 383, 459, 974, 1095, 1143
Alw26I GTCTC 2 cut(s) 117, 929
AlwI GGATC 5 cut(s) 767, 1233, 1246, 1310, 1323
AoxI GGCC 8 cut(s) 4, 15, 209, 480, 540, 912, 1006, 1341
ApeKI GCWGC 7 cut(s) 134, 350, 383, 608, 746, 1157, 1256
ApoI RAATTY 2 cut(s) 1044, 1272
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
Asp700I GAANNNNTTC 2 cut(s) 407, 1276
AspLEI GCGC 2 cut(s) 1024, 1034
AspS9I GGNCC 7 cut(s) 4, 15, 209, 480, 913, 1166, 1438
AsuC2I CCSGG 2 cut(s) 54, 484
AsuHPI GGTGA 1 cut(s) 1111
AsuII TTCGAA 2 cut(s) 879, 1233
AvaII GGWCC 2 cut(s) 1166, 1438
BaeGI GKGCMC 1 cut(s) 326
BalI TGGCCA 1 cut(s) 542
BamHI GGATCC 2 cut(s) 1238, 1315
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 7 cut(s) 121, 337, 370, 620, 733, 1144, 1243
BccI CCATC 7 cut(s) 37, 178, 220, 487, 576, 1120, 1369
BceAI ACGGC 1 cut(s) 1094
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BciT130I CCWGG 2 cut(s) 579, 635
BclI TGATCA 1 cut(s) 895
BcnI CCSGG 2 cut(s) 54, 484
BcoDI GTCTC 2 cut(s) 117, 929
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 4 cut(s) 284, 660, 1335, 1452
BfmI CTRYAG 1 cut(s) 351
BfoI RGCGCY 1 cut(s) 1035
BfuAI ACCTGC 1 cut(s) 266
BisI GCNGC 9 cut(s) 135, 351, 384, 609, 747, 912, 1009, 1158, 1257
BlsI GCNGC 9 cut(s) 136, 352, 385, 610, 748, 913, 1010, 1159, 1258
BmcAI AGTACT 1 cut(s) 733
Bme1390I CCNGG 4 cut(s) 54, 484, 579, 635
Bme18I GGWCC 2 cut(s) 1166, 1438
BmgT120I GGNCC 7 cut(s) 4, 15, 209, 480, 913, 1166, 1438
BmiI GGNNCC 4 cut(s) 481, 632, 1240, 1317
BmrFI CCNGG 4 cut(s) 54, 484, 579, 635
BmsI GCATC 4 cut(s) 272, 758, 1020, 1291
BpmI CTGGAG 3 cut(s) 4, 412, 589
Bpu10I CCTNAGC 1 cut(s) 1179
Bpu14I TTCGAA 2 cut(s) 879, 1233
BpuEI CTTGAG 3 cut(s) 363, 1341, 1463
BpuMI CCSGG 2 cut(s) 54, 484
BsaBI GATNNNNATC 2 cut(s) 28, 159
BsaJI CCNNGG 3 cut(s) 220, 845, 855
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 275, 489, 1004, 1268, 1325
Bse1I ACTGG 3 cut(s) 572, 1009, 1461
Bse3DI GCAATG 2 cut(s) 324, 447
Bse8I GATNNNNATC 2 cut(s) 28, 159
BseBI CCWGG 2 cut(s) 579, 635
BseDI CCNNGG 3 cut(s) 220, 845, 855
BseGI GGATG 4 cut(s) 498, 587, 715, 944
BseJI GATNNNNATC 2 cut(s) 28, 159
BseLI CCNNNNNNNGG 6 cut(s) 13, 275, 489, 1004, 1268, 1325
BseMI GCAATG 2 cut(s) 324, 447
BseMII CTCAG 3 cut(s) 605, 934, 1170
BseNI ACTGG 3 cut(s) 572, 1009, 1461
BseRI GAGGAG 2 cut(s) 155, 908
BseSI GKGCMC 1 cut(s) 326
BseXI GCAGC 7 cut(s) 121, 337, 370, 620, 733, 1144, 1243
BseYI CCCAGC 1 cut(s) 333
BsgI GTGCAG 2 cut(s) 272, 369
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 8 cut(s) 6, 17, 211, 482, 542, 914, 1008, 1343
BsiEI CGRYCG 1 cut(s) 301
BsiSI CCGG 2 cut(s) 54, 483
BslI CCNNNNNNNGG 6 cut(s) 13, 275, 489, 1004, 1268, 1325
BsmAI GTCTC 2 cut(s) 117, 929
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 8 cut(s) 6, 17, 211, 482, 542, 914, 1008, 1343
Bsp119I TTCGAA 2 cut(s) 879, 1233
Bsp1286I GDGCHC 1 cut(s) 326
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
Bsp19I CCATGG 2 cut(s) 845, 855
BspACI CCGC 5 cut(s) 301, 531, 911, 1009, 1079
BspANI GGCC 8 cut(s) 6, 17, 211, 482, 542, 914, 1008, 1343
BspCNI CTCAG 3 cut(s) 606, 933, 1171
BspLI GGNNCC 4 cut(s) 481, 632, 1240, 1317
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 266
BspPI GGATC 5 cut(s) 767, 1233, 1246, 1310, 1323
BspT104I TTCGAA 2 cut(s) 879, 1233
BsrDI GCAATG 2 cut(s) 324, 447
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 3 cut(s) 572, 1009, 1461
BssECI CCNNGG 3 cut(s) 220, 845, 855
BssMI GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
BssT1I CCWWGG 2 cut(s) 845, 855
Bst2UI CCWGG 2 cut(s) 579, 635
Bst4CI ACNGT 2 cut(s) 310, 905
Bst6I CTCTTC 3 cut(s) 960, 1252, 1470
BstAPI GCANNNNNTGC 2 cut(s) 330, 359
BstAUI TGTACA 1 cut(s) 177
BstBI TTCGAA 2 cut(s) 879, 1233
BstC8I GCNNGC 3 cut(s) 150, 364, 417
BstDEI CTNAG 3 cut(s) 614, 920, 1179
BstDSI CCRYGG 2 cut(s) 845, 855
BstF5I GGATG 4 cut(s) 498, 587, 715, 944
BstH2I RGCGCY 1 cut(s) 1035
BstHHI GCGC 2 cut(s) 1024, 1034
BstKTI GATC 6 cut(s) 762, 898, 979, 1225, 1241, 1318
BstMAI GTCTC 2 cut(s) 117, 929
BstMBI GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 7 cut(s) 203, 330, 359, 383, 528, 1157, 1457
BstNI CCWGG 2 cut(s) 579, 635
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 4 cut(s) 52, 482, 577, 633
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 326
BstV1I GCAGC 7 cut(s) 121, 337, 370, 620, 733, 1144, 1243
BstX2I RGATCY 2 cut(s) 1238, 1315
BstXI CCANNNNNNTGG 2 cut(s) 550, 1056
BstYI RGATCY 2 cut(s) 1238, 1315
BsuRI GGCC 8 cut(s) 6, 17, 211, 482, 542, 914, 1008, 1343
BtgI CCRYGG 2 cut(s) 845, 855
BtsCI GGATG 4 cut(s) 498, 587, 715, 944
BtsI GCAGTG 2 cut(s) 360, 405
BtsIMutI CAGTG 7 cut(s) 196, 306, 360, 405, 611, 1002, 1468
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 3 cut(s) 150, 364, 417
CfoI GCGC 2 cut(s) 1024, 1034
Cfr13I GGNCC 7 cut(s) 4, 15, 209, 480, 913, 1166, 1438
Csp6I GTAC 6 cut(s) 178, 281, 565, 575, 732, 906
CspCI CAANNNNNGTGG 4 cut(s) 295, 330, 1293, 1328
CviAII CATG 9 cut(s) 79, 562, 643, 683, 778, 846, 856, 1015, 1039
CviQI GTAC 6 cut(s) 178, 281, 565, 575, 732, 906
DdeI CTNAG 3 cut(s) 614, 920, 1179
DpnI GATC 6 cut(s) 761, 897, 978, 1224, 1240, 1317
DpnII GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
DraIII CACNNNGTG 1 cut(s) 873
EaeI YGGCCR 2 cut(s) 540, 1006
Eam1104I CTCTTC 3 cut(s) 960, 1252, 1470
EarI CTCTTC 3 cut(s) 960, 1252, 1470
Eco130I CCWWGG 2 cut(s) 845, 855
Eco147I AGGCCT 1 cut(s) 1343
Eco32I GATATC 1 cut(s) 227
Eco47I GGWCC 2 cut(s) 1166, 1438
Eco57I CTGAAG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 1438
EcoRI GAATTC 2 cut(s) 1044, 1272
EcoRII CCWGG 2 cut(s) 577, 633
EcoRV GATATC 1 cut(s) 227
EcoT14I CCWWGG 2 cut(s) 845, 855
EcoT22I ATGCAT 1 cut(s) 1219
ErhI CCWWGG 2 cut(s) 845, 855
FaeI CATG 9 cut(s) 82, 565, 646, 686, 781, 849, 859, 1018, 1042
FatI CATG 9 cut(s) 78, 561, 642, 682, 777, 845, 855, 1014, 1038
FauI CCCGC 1 cut(s) 1086
FbaI TGATCA 1 cut(s) 895
FblI GTMKAC 2 cut(s) 1089, 1331
Fnu4HI GCNGC 9 cut(s) 135, 351, 384, 609, 747, 912, 1009, 1158, 1257
FokI GGATG 4 cut(s) 505, 594, 722, 951
Fsp4HI GCNGC 9 cut(s) 135, 351, 384, 609, 747, 912, 1009, 1158, 1257
FspBI CTAG 4 cut(s) 284, 660, 1335, 1452
FspI TGCGCA 1 cut(s) 1023
GlaI GCGC 2 cut(s) 1023, 1033
GluI GCNGC 9 cut(s) 135, 351, 384, 609, 747, 912, 1009, 1158, 1257
GsaI CCCAGC 1 cut(s) 337
GsuI CTGGAG 3 cut(s) 4, 412, 589
HaeII RGCGCY 1 cut(s) 1035
HaeIII GGCC 8 cut(s) 6, 17, 211, 482, 542, 914, 1008, 1343
HapII CCGG 2 cut(s) 54, 483
HhaI GCGC 2 cut(s) 1024, 1034
Hin1II CATG 9 cut(s) 82, 565, 646, 686, 781, 849, 859, 1018, 1042
Hin6I GCGC 2 cut(s) 1022, 1032
HinP1I GCGC 2 cut(s) 1022, 1032
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 3 cut(s) 160, 764, 990
HpaII CCGG 2 cut(s) 54, 483
HphI GGTGA 1 cut(s) 1111
Hpy166II GTNNAC 5 cut(s) 178, 281, 655, 1090, 1332
Hpy188I TCNGA 5 cut(s) 217, 224, 1282, 1320, 1433
Hpy188III TCNNGA 4 cut(s) 21, 241, 391, 987
Hpy8I GTNNAC 5 cut(s) 178, 281, 655, 1090, 1332
HpyAV CCTTC 4 cut(s) 604, 1045, 1229, 1252
HpyCH4III ACNGT 2 cut(s) 310, 905
HpyCH4IV ACGT 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 7 cut(s) 203, 330, 359, 383, 528, 1157, 1457
HpyF3I CTNAG 3 cut(s) 614, 920, 1179
HpySE526I ACGT 1 cut(s) 67
Hsp92II CATG 9 cut(s) 82, 565, 646, 686, 781, 849, 859, 1018, 1042
HspAI GCGC 2 cut(s) 1022, 1032
Ksp22I TGATCA 1 cut(s) 895
Kzo9I GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
LmnI GCTCC 3 cut(s) 145, 570, 1264
Lsp1109I GCAGC 7 cut(s) 121, 337, 370, 620, 733, 1144, 1243
LweI GCATC 4 cut(s) 272, 758, 1020, 1291
MaeI CTAG 4 cut(s) 284, 660, 1335, 1452
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 4 cut(s) 557, 837, 984, 1111
MalI GATC 6 cut(s) 761, 897, 978, 1224, 1240, 1317
MboI GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
MboII GAAGA 6 cut(s) 24, 856, 947, 1239, 1297, 1487
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 2 cut(s) 1238, 1315
MhlI GDGCHC 1 cut(s) 326
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 7 cut(s) 420, 624, 665, 813, 1044, 1272, 1455
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 1 cut(s) 1249
Mox20I TGGCCA 1 cut(s) 542
Mph1103I ATGCAT 1 cut(s) 1219
MroXI GAANNNNTTC 2 cut(s) 407, 1276
MscI TGGCCA 1 cut(s) 542
MseI TTAA 4 cut(s) 9, 792, 1133, 1323
MslI CAYNNNNRTG 5 cut(s) 548, 602, 647, 844, 1124
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 2 cut(s) 911, 1143
MspI CCGG 2 cut(s) 54, 483
MspR9I CCNGG 4 cut(s) 54, 484, 579, 635
MunI CAATTG 1 cut(s) 420
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 2 cut(s) 579, 635
MwoI GCNNNNNNNGC 7 cut(s) 203, 330, 359, 383, 528, 1157, 1457
NciI CCSGG 2 cut(s) 54, 484
NcoI CCATGG 2 cut(s) 845, 855
NdeII GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
NlaIII CATG 9 cut(s) 82, 565, 646, 686, 781, 849, 859, 1018, 1042
NlaIV GGNNCC 4 cut(s) 481, 632, 1240, 1317
NmuCI GTSAC 3 cut(s) 837, 984, 1111
NsbI TGCGCA 1 cut(s) 1023
NsiI ATGCAT 1 cut(s) 1219
NspI RCATGY 1 cut(s) 565
NspV TTCGAA 2 cut(s) 879, 1233
PaqCI CACCTGC 1 cut(s) 266
PceI AGGCCT 1 cut(s) 1343
PciI ACATGT 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 985
PctI GAATGC 1 cut(s) 1153
PdmI GAANNNNTTC 2 cut(s) 407, 1276
PfeI GAWTC 2 cut(s) 160, 990
PkrI GCNGC 9 cut(s) 136, 352, 385, 610, 748, 913, 1010, 1159, 1258
PleI GAGTC 1 cut(s) 772
PpsI GAGTC 1 cut(s) 772
PpuMI RGGWCCY 1 cut(s) 1438
PscI ACATGT 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 1438
Psp6I CCWGG 2 cut(s) 577, 633
PspFI CCCAGC 1 cut(s) 333
PspGI CCWGG 2 cut(s) 577, 633
PspN4I GGNNCC 4 cut(s) 481, 632, 1240, 1317
PspPI GGNCC 7 cut(s) 4, 15, 209, 480, 913, 1166, 1438
PspPPI RGGWCCY 1 cut(s) 1438
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 2 cut(s) 1238, 1315
PvuII CAGCTG 1 cut(s) 1143
RsaI GTAC 6 cut(s) 179, 282, 566, 576, 733, 907
RsaNI GTAC 6 cut(s) 178, 281, 565, 575, 732, 906
RseI CAYNNNNRTG 5 cut(s) 548, 602, 647, 844, 1124
SaqAI TTAA 4 cut(s) 9, 792, 1133, 1323
SatI GCNGC 9 cut(s) 135, 351, 384, 609, 747, 912, 1009, 1158, 1257
Sau3AI GATC 6 cut(s) 759, 895, 976, 1222, 1238, 1315
Sau96I GGNCC 7 cut(s) 4, 15, 209, 480, 913, 1166, 1438
ScaI AGTACT 1 cut(s) 733
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 4 cut(s) 54, 484, 579, 635
SduI GDGCHC 1 cut(s) 326
SfaNI GCATC 4 cut(s) 272, 758, 1020, 1291
SfcI CTRYAG 1 cut(s) 351
SfuI TTCGAA 2 cut(s) 879, 1233
SinI GGWCC 2 cut(s) 1166, 1438
SmiMI CAYNNNNRTG 5 cut(s) 548, 602, 647, 844, 1124
SmlI CTYRAG 3 cut(s) 378, 1356, 1442
SmoI CTYRAG 3 cut(s) 378, 1356, 1442
SpeI ACTAGT 1 cut(s) 283
Sse9I AATT 7 cut(s) 420, 624, 665, 813, 1044, 1272, 1455
SseBI AGGCCT 1 cut(s) 1343
SsiI CCGC 5 cut(s) 301, 531, 911, 1009, 1079
SspMI CTAG 4 cut(s) 284, 660, 1335, 1452
StuI AGGCCT 1 cut(s) 1343
StyD4I CCNGG 4 cut(s) 52, 482, 577, 633
StyI CCWWGG 2 cut(s) 845, 855
TaaI ACNGT 2 cut(s) 310, 905
TaiI ACGT 1 cut(s) 70
TaqI TCGA 5 cut(s) 762, 879, 979, 1233, 1370
TasI AATT 7 cut(s) 420, 624, 665, 813, 1044, 1272, 1455
TatI WGTACW 5 cut(s) 177, 280, 564, 731, 905
TauI GCSGC 2 cut(s) 914, 1011
TfiI GAWTC 2 cut(s) 160, 990
Tru1I TTAA 4 cut(s) 9, 792, 1133, 1323
Tru9I TTAA 4 cut(s) 9, 792, 1133, 1323
TscAI CASTG 7 cut(s) 196, 313, 360, 405, 618, 1009, 1468
TseFI GTSAC 3 cut(s) 837, 984, 1111
TseI GCWGC 7 cut(s) 134, 350, 383, 608, 746, 1157, 1256
Tsp45I GTSAC 3 cut(s) 837, 984, 1111
TspDTI ATGAA 6 cut(s) 226, 436, 586, 631, 794, 1027
TspRI CASTG 7 cut(s) 196, 313, 360, 405, 618, 1009, 1468
VpaK11BI GGWCC 2 cut(s) 1166, 1438
XapI RAATTY 2 cut(s) 1044, 1272
XceI RCATGY 1 cut(s) 565
XmiI GTMKAC 2 cut(s) 1089, 1331
XmnI GAANNNNTTC 2 cut(s) 407, 1276
XspI CTAG 4 cut(s) 284, 660, 1335, 1452
ZrmI AGTACT 1 cut(s) 733
Zsp2I ATGCAT 1 cut(s) 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.