pycom02g12550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016732
Physical Location & Seq
Reverse (-)
9188525 .. 9188866
342 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12550.1

Sequence Viewer

Length: 342 bp
ATGGCTTTCATGATTTCCAAAGCTCAACAGCAACAACCATCAACTTTCTCAGAAGCTGCTTCTGGGAAGATCAAAGATGGCCATGAAGTCTCTCCTGAGTCACAGAGCTCCATCACAAGCCACGTCTACTTGAAATCACCCACTGCAGCTTCCTTGGACAGAGATGTTGTTCTCAGACGCATTCGCAACCATAAGCACTTGAGCAAGGTTAAAAGTACTTTTAAAGCTTTCATGGGAAGCAGCAGGAGCTCTCCAGAACCTGCTACTAGTCCTAGGACCCCTACTTCTAACATCCACCATGAGACATGGCTGCACCAGGAGGACTCTTTCTCCTTCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.58

Weight (kDa)

9.69

Isoelectric Point (pI)

77.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14560
prunus_persica Prupe.7G144800_v2.0.a1 Prupe.7G144900_v2.0.a1
pyrus_communis pycom02g12550
rosa_chinensis RchiOBHm_Chr2g0103311
rosa_laevigata RLG00000017222
rosa_multiflora Rmu_sc0006743.1_g000006 Rmu_sc0013125.1_g000001
rosa_roxburghii Rroxscaffold_2G00139750
rosa_rugosa Rorug02G0113400
rosa_samantha Rh2AG162100 Rh2BG169100 Rh2CG169000 Rh2DG167900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccI GTMKAC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 79
AfaI GTAC 1 cut(s) 217
AgsI TTSAA 1 cut(s) 133
AhlI ACTAGT 1 cut(s) 266
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 1 cut(s) 315
AluBI AGCT 6 cut(s) 23, 56, 108, 149, 227, 249
AluI AGCT 6 cut(s) 23, 56, 108, 149, 227, 249
Alw21I GWGCWC 2 cut(s) 110, 251
Alw26I GTCTC 2 cut(s) 94, 296
AlwNI CAGNNNCTG 2 cut(s) 56, 260
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 4 cut(s) 56, 146, 240, 310
AspA2I CCTAGG 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 129
AvaII GGWCC 1 cut(s) 276
AvrII CCTAGG 1 cut(s) 272
BalI TGGCCA 1 cut(s) 81
BanII GRGCYC 2 cut(s) 110, 251
Bbv12I GWGCWC 2 cut(s) 110, 251
BbvI GCAGC 4 cut(s) 43, 158, 252, 297
BccI CCATC 3 cut(s) 46, 71, 119
BciT130I CCWGG 1 cut(s) 317
BcoDI GTCTC 2 cut(s) 94, 296
BcuI ACTAGT 1 cut(s) 266
BfaI CTAG 2 cut(s) 267, 273
BfmI CTRYAG 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 57, 147, 241, 311
BlnI CCTAGG 1 cut(s) 272
BlsI GCNGC 4 cut(s) 58, 148, 242, 312
BmcAI AGTACT 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 276
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 1 cut(s) 278
BmrFI CCNGG 1 cut(s) 317
BpmI CTGGAG 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 153, 272
BsaXI ACNNNNNCTCC 1 cut(s) 314
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 153, 272
BseGI GGATG 1 cut(s) 291
BseMII CTCAG 3 cut(s) 63, 87, 187
BseXI GCAGC 4 cut(s) 43, 158, 252, 297
BsgI GTGCAG 1 cut(s) 296
BshFI GGCC 1 cut(s) 81
BsiHKAI GWGCWC 2 cut(s) 110, 251
BsmAI GTCTC 2 cut(s) 94, 296
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 110, 251
Bsp143I GATC 1 cut(s) 69
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 3 cut(s) 62, 88, 186
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 278
BspMAI CTGCAG 1 cut(s) 148
BspMI ACCTGC 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 153, 272
BssMI GATC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 153, 272
Bst2UI CCWGG 1 cut(s) 317
BstDEI CTNAG 4 cut(s) 49, 96, 173, 339
BstF5I GGATG 1 cut(s) 291
BstKTI GATC 1 cut(s) 72
BstMAI GTCTC 2 cut(s) 94, 296
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 43, 158, 252, 297
BsuRI GGCC 1 cut(s) 81
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 1 cut(s) 291
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 268
CaiI CAGNNNCTG 2 cut(s) 56, 260
CciI TCATGA 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 276
CseI GACGC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 216
CviAII CATG 5 cut(s) 10, 83, 232, 299, 306
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 4 cut(s) 49, 96, 173, 339
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
DraI TTTAAA 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 79
Ecl136II GAGCTC 2 cut(s) 108, 249
Eco130I CCWWGG 2 cut(s) 153, 272
Eco24I GRGCYC 2 cut(s) 110, 251
Eco47I GGWCC 1 cut(s) 276
Eco53kI GAGCTC 2 cut(s) 108, 249
EcoICRI GAGCTC 2 cut(s) 108, 249
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 315
EcoT14I CCWWGG 2 cut(s) 153, 272
EcoT38I GRGCYC 2 cut(s) 110, 251
ErhI CCWWGG 2 cut(s) 153, 272
FaeI CATG 5 cut(s) 13, 86, 235, 302, 309
FaiI YATR 6 cut(s) 11, 84, 192, 233, 300, 307
FatI CATG 5 cut(s) 9, 82, 231, 298, 305
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 57, 147, 241, 311
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 2 cut(s) 110, 251
Fsp4HI GCNGC 4 cut(s) 57, 147, 241, 311
FspBI CTAG 2 cut(s) 267, 273
GluI GCNGC 4 cut(s) 57, 147, 241, 311
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 1 cut(s) 81
HgaI GACGC 1 cut(s) 186
Hin1II CATG 5 cut(s) 13, 86, 235, 302, 309
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 2 cut(s) 98, 323
HphI GGTGA 1 cut(s) 129
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 52, 176
Hpy188III TCNNGA 3 cut(s) 10, 95, 254
Hpy8I GTNNAC 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 146, 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 4 cut(s) 49, 96, 173, 339
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 5 cut(s) 13, 86, 235, 302, 309
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 113, 246
LpnPI CCDG 7 cut(s) 48, 108, 229, 267, 273, 302, 329
Lsp1109I GCAGC 4 cut(s) 43, 158, 252, 297
MaeI CTAG 2 cut(s) 267, 273
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 99
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 1 cut(s) 79
MhlI GDGCHC 2 cut(s) 110, 251
MlsI TGGCCA 1 cut(s) 81
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 2 cut(s) 107, 317
MnlI CCTC 1 cut(s) 313
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 2 cut(s) 210, 222
Msp20I TGGCCA 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 1 cut(s) 317
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 1 cut(s) 69
NlaIII CATG 5 cut(s) 13, 86, 235, 302, 309
NlaIV GGNNCC 1 cut(s) 278
NmuCI GTSAC 1 cut(s) 99
PagI TCATGA 1 cut(s) 9
PctI GAATGC 1 cut(s) 180
PkrI GCNGC 4 cut(s) 58, 148, 242, 312
PleI GAGTC 2 cut(s) 106, 317
PpsI GAGTC 2 cut(s) 106, 317
PpuMI RGGWCCY 1 cut(s) 276
Psp124BI GAGCTC 2 cut(s) 110, 251
Psp5II RGGWCCY 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 315
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 1 cut(s) 278
PspPI GGNCC 1 cut(s) 276
PspPPI RGGWCCY 1 cut(s) 276
PstI CTGCAG 1 cut(s) 148
PstNI CAGNNNCTG 2 cut(s) 56, 260
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
SacI GAGCTC 2 cut(s) 110, 251
SaqAI TTAA 2 cut(s) 210, 222
SatI GCNGC 4 cut(s) 57, 147, 241, 311
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 276
ScaI AGTACT 1 cut(s) 217
SchI GAGTC 2 cut(s) 107, 317
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 2 cut(s) 110, 251
SetI ASST 9 cut(s) 25, 58, 110, 126, 151, 210, 229, 251, 262
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
SpeI ACTAGT 1 cut(s) 266
SspMI CTAG 2 cut(s) 267, 273
SstI GAGCTC 2 cut(s) 110, 251
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 2 cut(s) 153, 272
TaiI ACGT 1 cut(s) 126
TatI WGTACW 1 cut(s) 215
Tru1I TTAA 2 cut(s) 210, 222
Tru9I TTAA 2 cut(s) 210, 222
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 99
TseI GCWGC 4 cut(s) 56, 146, 240, 310
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 2 cut(s) 99, 220
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 276
XmaJI CCTAGG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 2 cut(s) 267, 273
ZrmI AGTACT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.