pycom02g12890

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0021253
Physical Location & Seq
Forward (+)
9465444 .. 9466658
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12890.3

Sequence Viewer

Length: 759 bp
ATGGCAACGGTAAATGAGTTGATTAATCCAAGTGCAAAAAGTTGGAAGATTAATATGCTAGAAAACCTCTTCTCTACTGAGGAGGTAAATTTGATCCGGACAATTCCATTAAGTGCTCGAAATCCGCCGGATCACTCGTTGTGGCATCACGAGAGACATGGAAGGTTTACGGTCTGGTCGGCCTACCATGTGGCGAGGAATTGGCTTCGGCCCACAAACCAGGCAGCATCATCCTCTAACGCTAATGGTTCTAAGATTTGGGCAAAAGTGTGGAACGCAAACGTGCCACCGAAAGCAAGCTTTGATGTGTTTCTGATGGTTTGTTGGGCATTATGGGGGGCACGGAACAAGAAACTCTGGGACGATAAGCTGGTGTCTCCTGAGATATGCACTGCTAGTGCTATTCAATGGTGGTTGGAGTTTACCAAAATCACAAATCCAGAGAGCTTAGTTGCTGGTTTCGGTGCCATTATCAGGGATGGTGATGGTCTATTTGTGGCTGCTAGTATTGGAAGTTTTGAAGACATAGCCTCCCTACTGCTCTCGGAAGCTATGGCAATGAGGGCAGGACAAGTTGTGGAGGATTGCAAAGCGTTACTCAATACAATCACTGAAGCAAATATCACCCACAGTCGCCGAAATGCAAACACTGCTGCTCACCACCTTGCAAAAGTCGGCCTGTCTTTGATGCAAAGCTGTGAATGGATCGATTCTCCACCTAGTATCATAACTGATATTATTGTTGAGGACTGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

253

Amino Acids

28.04

Weight (kDa)

6.24

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021253)

Species Orthologous Gene IDs
pyrus_communis pycom02g12890
rosa_multiflora Rmu_sc0011259.1_g000007
rosa_rugosa Rorug03G0130000.1 Rorug07G0003500
rosa_samantha Rh5BG567600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AccIII TCCGGA 1 cut(s) 96
AciI CCGC 1 cut(s) 125
AclWI GGATC 3 cut(s) 88, 138, 713
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 633
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 2 cut(s) 407, 521
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 5 cut(s) 300, 370, 447, 551, 696
AluI AGCT 5 cut(s) 300, 370, 447, 551, 696
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 148, 381
AlwI GGATC 3 cut(s) 88, 138, 713
Aor13HI TCCGGA 1 cut(s) 96
AoxI GGCC 3 cut(s) 180, 209, 676
ApeKI GCWGC 3 cut(s) 224, 500, 653
ApoI RAATTY 1 cut(s) 88
AseI ATTAAT 2 cut(s) 24, 51
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 494, 616, 650
BaeGI GKGCMC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 464
BauI CACGAG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 236, 487, 640
BccI CCATC 3 cut(s) 310, 473, 479
BciT130I CCWGG 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 148, 381
BfaI CTAG 4 cut(s) 59, 396, 504, 720
BisI GCNGC 3 cut(s) 225, 501, 654
BlsI GCNGC 3 cut(s) 226, 502, 655
Bme1390I CCNGG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 3 cut(s) 154, 236, 678
BpiI GAAGAC 1 cut(s) 528
Bsa29I ATCGAT 1 cut(s) 708
BsaWI WCCGGW 1 cut(s) 96
BsaXI ACNNNNNCTCC 2 cut(s) 515, 545
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 96
BseBI CCWGG 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 708
BseGI GGATG 2 cut(s) 230, 484
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 2 cut(s) 69, 372
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 3 cut(s) 236, 487, 640
BshFI GGCC 3 cut(s) 182, 211, 678
BshNI GGYRCC 1 cut(s) 464
BshVI ATCGAT 1 cut(s) 708
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 97, 128
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 2 cut(s) 148, 381
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 3 cut(s) 182, 211, 678
Bsp1286I GDGCHC 2 cut(s) 118, 343
Bsp13I TCCGGA 1 cut(s) 96
Bsp143I GATC 3 cut(s) 93, 130, 705
BspACI CCGC 1 cut(s) 125
BspANI GGCC 3 cut(s) 182, 211, 678
BspCNI CTCAG 2 cut(s) 70, 373
BspDI ATCGAT 1 cut(s) 708
BspEI TCCGGA 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 466
BspPI GGATC 3 cut(s) 88, 138, 713
BspT107I GGYRCC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 564
BssMI GATC 3 cut(s) 93, 130, 705
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 4 cut(s) 10, 172, 632, 752
Bst6I CTCTTC 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 4 cut(s) 78, 252, 381, 448
BstF5I GGATG 2 cut(s) 230, 484
BstKTI GATC 3 cut(s) 96, 133, 708
BstMAI GTCTC 2 cut(s) 148, 381
BstMBI GATC 3 cut(s) 93, 130, 705
BstMWI GCNNNNNNNGC 2 cut(s) 563, 650
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 343
BstV1I GCAGC 3 cut(s) 236, 487, 640
BstV2I GAAGAC 1 cut(s) 528
Bsu15I ATCGAT 1 cut(s) 708
BsuRI GGCC 3 cut(s) 182, 211, 678
BsuTUI ATCGAT 1 cut(s) 708
BtsCI GGATG 2 cut(s) 230, 484
BtsI GCAGTG 2 cut(s) 390, 648
BtsIMutI CAGTG 3 cut(s) 390, 609, 648
Cac8I GCNNGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 210
ClaI ATCGAT 1 cut(s) 708
CviAII CATG 2 cut(s) 158, 188
DdeI CTNAG 4 cut(s) 78, 252, 381, 448
DpnI GATC 3 cut(s) 95, 132, 707
DpnII GATC 3 cut(s) 93, 130, 705
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
EciI GGCGGA 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 633
EcoRII CCWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 161, 191
FaiI YATR 9 cut(s) 56, 159, 189, 334, 388, 527, 554, 728, 755
FaqI GGGAC 1 cut(s) 374
FatI CATG 2 cut(s) 157, 187
Fnu4HI GCNGC 3 cut(s) 225, 501, 654
FokI GGATG 2 cut(s) 217, 491
Fsp4HI GCNGC 3 cut(s) 225, 501, 654
FspBI CTAG 4 cut(s) 59, 396, 504, 720
GluI GCNGC 3 cut(s) 225, 501, 654
HaeIII GGCC 3 cut(s) 182, 211, 678
HapII CCGG 2 cut(s) 97, 128
Hin1II CATG 2 cut(s) 161, 191
HindIII AAGCTT 1 cut(s) 298
HinfI GANTC 1 cut(s) 710
HpaII CCGG 2 cut(s) 97, 128
HphI GGTGA 3 cut(s) 494, 616, 650
Hpy166II GTNNAC 2 cut(s) 168, 423
Hpy188I TCNGA 2 cut(s) 315, 547
Hpy188III TCNNGA 4 cut(s) 97, 149, 380, 440
Hpy8I GTNNAC 2 cut(s) 168, 423
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 4 cut(s) 10, 172, 632, 752
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 6 cut(s) 35, 390, 588, 644, 668, 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 650
HpyF3I CTNAG 4 cut(s) 78, 252, 381, 448
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 2 cut(s) 161, 191
Kpn2I TCCGGA 1 cut(s) 96
Kzo9I GATC 3 cut(s) 93, 130, 705
Lsp1109I GCAGC 3 cut(s) 236, 487, 640
LweI GCATC 3 cut(s) 154, 236, 678
MaeI CTAG 4 cut(s) 59, 396, 504, 720
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 594
MalI GATC 3 cut(s) 95, 132, 707
MboI GATC 3 cut(s) 93, 130, 705
MboII GAAGA 3 cut(s) 58, 61, 533
MhlI GDGCHC 2 cut(s) 118, 343
MluCI AATT 3 cut(s) 88, 102, 199
MmeI TCCRAC 2 cut(s) 23, 396
MnlI CCTC 9 cut(s) 73, 76, 77, 189, 244, 541, 555, 574, 739
MroI TCCGGA 1 cut(s) 96
MseI TTAA 3 cut(s) 24, 51, 110
MspI CCGG 2 cut(s) 97, 128
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 563, 650
NdeII GATC 3 cut(s) 93, 130, 705
NlaIII CATG 2 cut(s) 161, 191
NlaIV GGNNCC 1 cut(s) 466
PfeI GAWTC 1 cut(s) 710
PkrI GCNGC 3 cut(s) 226, 502, 655
PshBI ATTAAT 2 cut(s) 24, 51
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 466
PspPI GGNCC 1 cut(s) 210
SaqAI TTAA 3 cut(s) 24, 51, 110
SatI GCNGC 3 cut(s) 225, 501, 654
Sau3AI GATC 3 cut(s) 93, 130, 705
Sau96I GGNCC 1 cut(s) 210
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 2 cut(s) 118, 343
SfaNI GCATC 3 cut(s) 154, 236, 678
Sse9I AATT 3 cut(s) 88, 102, 199
SsiI CCGC 1 cut(s) 125
SspMI CTAG 4 cut(s) 59, 396, 504, 720
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 10, 172, 632, 752
TaiI ACGT 1 cut(s) 285
TaqI TCGA 2 cut(s) 118, 708
TasI AATT 3 cut(s) 88, 102, 199
TfiI GAWTC 1 cut(s) 710
Tru1I TTAA 3 cut(s) 24, 51, 110
Tru9I TTAA 3 cut(s) 24, 51, 110
TscAI CASTG 3 cut(s) 397, 616, 655
TseI GCWGC 3 cut(s) 224, 500, 653
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 3 cut(s) 397, 616, 655
VspI ATTAAT 2 cut(s) 24, 51
XapI RAATTY 1 cut(s) 88
XspI CTAG 4 cut(s) 59, 396, 504, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.