pycom02g20000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022447
Physical Location & Seq
Reverse (-)
18126973 .. 18127317
345 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 345 bp
ATGAGCAGCAGAGGGCCGTCGCCACTTGTACCTTCTCTATTGGTGGGTGCATTGGGTTTGATCGTCTGCAGACCTACCATAATATCCCTCCTGGAAATCTCAGTGTCTGTGTTCCAAATAGGTGCAGAGACAGAGCGGAGAACCACCTTTGCTTTTGTGATGCTTCTTCTTCTCTTAGTTTTACTGGTTGTGCACGTTCTTGCTTCATTTTTTCCATCGTTTCAGATGTCATCCCCCGAGGTTCATCACACGAGTACCTCGGAACACGGTGGTGACGGGCTCGGGTTCGGAGTGGGATCGTTTCTTCTACTTGTACTCTTCCTTGTTCTATTTCATCTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

115

Amino Acids

12.23

Weight (kDa)

6.15

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022447)

Species Orthologous Gene IDs
prunus_persica Prupe.2G107100_v2.0.a1
pyrus_communis pycom02g20000
rosa_laevigata RLG00000029044
rosa_roxburghii Rroxscaffold_4G00311480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 136
AciI CCGC 1 cut(s) 136
AclWI GGATC 1 cut(s) 304
AfaI GTAC 3 cut(s) 30, 256, 315
AjnI CCWGG 1 cut(s) 90
AleI CACNNNNGTG 1 cut(s) 270
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 122
Alw44I GTGCAC 1 cut(s) 191
AlwI GGATC 1 cut(s) 304
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 2 cut(s) 236, 281
AoxI GGCC 1 cut(s) 14
ApaLI GTGCAC 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 6
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 1 cut(s) 284
AvaI CYCGRG 2 cut(s) 236, 281
BaeGI GKGCMC 1 cut(s) 195
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BanII GRGCYC 1 cut(s) 282
BauI CACGAG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 18
BccI CCATC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 67
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 92
BmeT110I CYCGRG 2 cut(s) 236, 281
BmgT120I GGNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 237, 258
Bse1I ACTGG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 237, 258
BseGI GGATG 1 cut(s) 230
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 189
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 1 cut(s) 18
BsgI GTGCAG 1 cut(s) 144
BshFI GGCC 1 cut(s) 16
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 2 cut(s) 236, 281
BsmAI GTCTC 1 cut(s) 122
BsnI GGCC 1 cut(s) 16
BsoBI CYCGRG 2 cut(s) 236, 281
Bsp1286I GDGCHC 2 cut(s) 195, 282
Bsp143I GATC 2 cut(s) 60, 296
BspACI CCGC 1 cut(s) 136
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 1 cut(s) 113
BspMAI CTGCAG 1 cut(s) 71
BspPI GGATC 1 cut(s) 304
BsrBI CCGCTC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 189
BssECI CCNNGG 2 cut(s) 237, 258
BssMI GATC 2 cut(s) 60, 296
BssSI CACGAG 1 cut(s) 250
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 323
BstDEI CTNAG 2 cut(s) 100, 175
BstF5I GGATG 1 cut(s) 230
BstKTI GATC 2 cut(s) 63, 299
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 60, 296
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 18
BsuRI GGCC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 3 cut(s) 29, 255, 314
CviJI RGCY 2 cut(s) 16, 280
CviKI_1 RGCY 2 cut(s) 16, 280
CviQI GTAC 3 cut(s) 29, 255, 314
DdeI CTNAG 2 cut(s) 100, 175
DpnI GATC 2 cut(s) 62, 298
DpnII GATC 2 cut(s) 60, 296
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 236, 281
EcoRII CCWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 282
FaiI YATR 1 cut(s) 80
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 7
GluI GCNGC 1 cut(s) 7
HaeIII GGCC 1 cut(s) 16
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 225, 262, 290
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 1 cut(s) 42
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 4 cut(s) 50, 69, 125, 193
HpyF3I CTNAG 2 cut(s) 100, 175
HpySE526I ACGT 1 cut(s) 195
Kzo9I GATC 2 cut(s) 60, 296
LpnPI CCDG 4 cut(s) 77, 104, 170, 323
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 150
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 272
MalI GATC 2 cut(s) 62, 298
MbiI CCGCTC 1 cut(s) 136
MboI GATC 2 cut(s) 60, 296
MboII GAAGA 4 cut(s) 158, 161, 296, 310
MhlI GDGCHC 2 cut(s) 195, 282
MnlI CCTC 4 cut(s) 5, 98, 232, 268
MslI CAYNNNNRTG 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
NdeII GATC 2 cut(s) 60, 296
NmuCI GTSAC 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspPI GGNCC 1 cut(s) 14
PstI CTGCAG 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 107
RsaI GTAC 3 cut(s) 30, 256, 315
RsaNI GTAC 3 cut(s) 29, 255, 314
RseI CAYNNNNRTG 1 cut(s) 270
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 2 cut(s) 60, 296
Sau96I GGNCC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 195, 282
SetI ASST 7 cut(s) 34, 76, 124, 149, 198, 243, 260
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 270
SsiI CCGC 1 cut(s) 136
StyD4I CCNGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 1 cut(s) 198
TatI WGTACW 1 cut(s) 313
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 3 cut(s) 195, 233, 323
TspRI CASTG 1 cut(s) 108
VneI GTGCAC 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.