pycom03g01650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0022362
Physical Location & Seq
Forward (+)
1220479 .. 1223131
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g01650.1

Sequence Viewer

Length: 1083 bp
ATGAGAATTCGAAATAGCCGGTCAAATTTTTCTCAGATGACTCCACCTGAGACCACACCCAGGGCTGCTCATCTTTGTTCACCAGAAGGTTCAGATGATCGCTTTTTAGTTGAAGAAGAGAATCGGCGCATTGGATTGTCCTGCCATACAAAAAAACAATTTCCTGCTCAAGTTTCAAGCGAAGATCAATTGCATGATGAATTGGAAGCAGCAAGCAGTACAGAAATTAGGCCTTTGAACTGTGTTGATAAGGGGAAAGATGCGAAACATTGCATGCAAATGGATACAAATCCGGCCTCAGAGGGGCAGAGCGAAGGAGAAGCAGATAAAGGGAAACAATTTACTGCATGTGCTGGTTCTCAGGGGGGTGGAGAAAACGTAACTTCTCCAGTTACAGCTGGGATCGCTCGTCCTTGTTCTTTTTCTCATCTAGGTCAGAAACTTCCGTTTAAGAAAAGAAGACCTTATAGTCACACAGAGACTGAAGTAGAGATGGAAAGAGAGAAAGTAGAAGATGTCTATCTAGAAGTAAAAGTGGAAGATGCGGTAATGGAAGCAAAAGAAAAATGCATGCCAGTAGAAATTGAAAAAGAAAATCATTTTGCAGAAGTTAAAGAAGATGAGCCAGTTAAAGCAGAAGCTGAAGATGCAGTAGCTGATAAGAAAGAGGAATCACTGGAGGAAGGCGAGATATGTGAAGAAAATGCGGAGGAGACAGAAAAAACAAGTGAGCTTGAACAGGTCAATGAAGGGTTGTATGTATCTCGTCTGACCATTGATGAGAACCAGCAGGAGAGCAAAAATGAAGAACTTAACCTAGAAGAGACAGCAAGTGAACCAAATGAGAAGCCAAGAAGGGGAAGGAAAACGCCCAGATCTAGTGCTAGCACTAGCAGCGATGAACCTCATCAGGGAAGGCAGAAGCTAAAGAGAGGAAGTCCAAAGAGGATGCAAGATCCAGCTGAGGCAAATCAAGGGTTTTGTCACATCCCTCCCCCCCCCTATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACGTGTGTGTGTGTGTGTGTTTGGACATGGATTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

361

Amino Acids

40.09

Weight (kDa)

4.98

Isoelectric Point (pI)

59.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022362)

Species Orthologous Gene IDs
malus_domestica MD03G1018900.v1.1 MD11G1020900.v1.1
pyrus_communis pycom03g01650 pycom11g01680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 468
AciI CCGC 2 cut(s) 545, 707
AclWI GGATC 2 cut(s) 410, 950
AcsI RAATTY 2 cut(s) 6, 25
AcuI CTGAAG 2 cut(s) 504, 663
AcvI CACGTG 1 cut(s) 1046
AfaI GTAC 1 cut(s) 220
AfiI CCNNNNNNNGG 4 cut(s) 60, 303, 857, 911
AflIII ACRYGT 1 cut(s) 1045
AgsI TTSAA 5 cut(s) 113, 177, 238, 587, 737
AjnI CCWGG 1 cut(s) 59
AleI CACNNNNGTG 1 cut(s) 1048
AluBI AGCT 6 cut(s) 398, 641, 656, 733, 925, 962
AluI AGCT 6 cut(s) 398, 641, 656, 733, 925, 962
Alw26I GTCTC 4 cut(s) 44, 473, 707, 818
AlwI GGATC 2 cut(s) 410, 950
AlwNI CAGNNNCTG 3 cut(s) 482, 641, 656
AoxI GGCC 2 cut(s) 230, 294
ApeKI GCWGC 3 cut(s) 65, 209, 894
ApoI RAATTY 2 cut(s) 6, 25
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 72
AsuII TTCGAA 1 cut(s) 10
AsuNHI GCTAGC 1 cut(s) 884
BbrPI CACGTG 1 cut(s) 1046
BbsI GAAGAC 1 cut(s) 466
BbvCI CCTCAGC 1 cut(s) 963
BbvI GCAGC 3 cut(s) 52, 221, 906
BccI CCATC 1 cut(s) 487
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 277
BcoDI GTCTC 4 cut(s) 44, 473, 707, 818
BfaI CTAG 6 cut(s) 431, 524, 818, 879, 885, 891
BfuI GTATCC 1 cut(s) 277
BglII AGATCT 1 cut(s) 875
BisI GCNGC 3 cut(s) 66, 210, 895
BlsI GCNGC 3 cut(s) 67, 211, 896
Bme1390I CCNGG 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 4 cut(s) 250, 532, 637, 939
BmtI GCTAGC 1 cut(s) 888
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 2 cut(s) 372, 698
Bpu10I CCTNAGC 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 1046
BsaBI GATNNNNATC 2 cut(s) 288, 519
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 59, 60
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 303, 857, 911
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 4 cut(s) 389, 575, 626, 681
Bse3DI GCAATG 1 cut(s) 268
Bse8I GATNNNNATC 2 cut(s) 288, 519
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 59, 60
BseGI GGATG 2 cut(s) 954, 987
BseJI GATNNNNATC 2 cut(s) 288, 519
BseLI CCNNNNNNNGG 4 cut(s) 60, 303, 857, 911
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 5 cut(s) 39, 47, 312, 374, 954
BseNI ACTGG 4 cut(s) 389, 575, 626, 681
BseRI GAGGAG 1 cut(s) 725
BseXI GCAGC 3 cut(s) 52, 221, 906
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 2 cut(s) 232, 296
BsiSI CCGG 2 cut(s) 19, 293
BslI CCNNNNNNNGG 4 cut(s) 60, 303, 857, 911
BsmAI GTCTC 4 cut(s) 44, 473, 707, 818
BsnI GGCC 2 cut(s) 232, 296
Bso31I GGTCTC 1 cut(s) 44
Bsp119I TTCGAA 1 cut(s) 10
Bsp143I GATC 5 cut(s) 97, 184, 402, 875, 955
BspACI CCGC 2 cut(s) 545, 707
BspANI GGCC 2 cut(s) 232, 296
BspCNI CTCAG 5 cut(s) 40, 46, 311, 373, 955
BspOI GCTAGC 1 cut(s) 888
BspPI GGATC 2 cut(s) 410, 950
BspT104I TTCGAA 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 268
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 4 cut(s) 389, 575, 626, 681
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 2 cut(s) 59, 60
BssMI GATC 5 cut(s) 97, 184, 402, 875, 955
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 2 cut(s) 111, 816
BstBAI YACGTR 1 cut(s) 1046
BstBI TTCGAA 1 cut(s) 10
BstC8I GCNNGC 4 cut(s) 214, 275, 572, 886
BstDEI CTNAG 5 cut(s) 33, 48, 298, 360, 963
BstF5I GGATG 2 cut(s) 954, 987
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 100, 187, 405, 878, 958
BstMAI GTCTC 4 cut(s) 44, 473, 707, 818
BstMBI GATC 5 cut(s) 97, 184, 402, 875, 955
BstMWI GCNNNNNNNGC 3 cut(s) 404, 647, 894
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 3 cut(s) 277, 351, 574
BstSCI CCNGG 1 cut(s) 59
BstV1I GCAGC 3 cut(s) 52, 221, 906
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 2 cut(s) 875, 955
BstYI RGATCY 2 cut(s) 875, 955
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 2 cut(s) 232, 296
BtgZI GCGATG 1 cut(s) 912
BtsCI GGATG 2 cut(s) 954, 987
BtsIMutI CAGTG 1 cut(s) 674
Cac8I GCNNGC 4 cut(s) 214, 275, 572, 886
CaiI CAGNNNCTG 3 cut(s) 482, 641, 656
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 18
Csp6I GTAC 1 cut(s) 219
CviAII CATG 5 cut(s) 194, 274, 348, 571, 1070
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 5 cut(s) 33, 48, 298, 360, 963
DpnI GATC 5 cut(s) 99, 186, 404, 877, 957
DpnII GATC 5 cut(s) 97, 184, 402, 875, 955
DrdI GACNNNNNNGTC 1 cut(s) 468
DseDI GACNNNNNNGTC 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 111, 816
EarI CTCTTC 2 cut(s) 111, 816
Eco147I AGGCCT 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 504, 663
Eco72I CACGTG 1 cut(s) 1046
EcoRI GAATTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 572
FaeI CATG 5 cut(s) 197, 277, 351, 574, 1073
FaiI YATR 9 cut(s) 147, 195, 275, 349, 468, 572, 694, 759, 1071
FalI AAGNNNNNCTT 2 cut(s) 448, 480
FatI CATG 5 cut(s) 193, 273, 347, 570, 1069
Fnu4HI GCNGC 3 cut(s) 66, 210, 895
FokI GGATG 2 cut(s) 961, 974
Fsp4HI GCNGC 3 cut(s) 66, 210, 895
FspBI CTAG 6 cut(s) 431, 524, 818, 879, 885, 891
GlaI GCGC 1 cut(s) 128
GluI GCNGC 3 cut(s) 66, 210, 895
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 372, 698
HaeIII GGCC 2 cut(s) 232, 296
HapII CCGG 2 cut(s) 19, 293
HhaI GCGC 1 cut(s) 129
Hin1II CATG 5 cut(s) 197, 277, 351, 574, 1073
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 3 cut(s) 40, 121, 671
HpaII CCGG 2 cut(s) 19, 293
HphI GGTGA 1 cut(s) 72
Hpy166II GTNNAC 2 cut(s) 80, 836
Hpy188I TCNGA 5 cut(s) 36, 94, 301, 438, 771
Hpy188III TCNNGA 1 cut(s) 524
Hpy8I GTNNAC 2 cut(s) 80, 836
HpyAV CCTTC 7 cut(s) 80, 308, 677, 743, 849, 855, 909
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 2 cut(s) 378, 1045
HpyCH4V TGCA 8 cut(s) 193, 273, 277, 347, 570, 605, 650, 952
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 647, 894
HpyF3I CTNAG 5 cut(s) 33, 48, 298, 360, 963
HpySE526I ACGT 2 cut(s) 378, 1045
Hsp92II CATG 5 cut(s) 197, 277, 351, 574, 1073
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 5 cut(s) 97, 184, 402, 875, 955
Lsp1109I GCAGC 3 cut(s) 52, 221, 906
LweI GCATC 4 cut(s) 250, 532, 637, 939
MaeI CTAG 6 cut(s) 431, 524, 818, 879, 885, 891
MaeII ACGT 2 cut(s) 378, 1045
MaeIII GTNAC 4 cut(s) 379, 391, 470, 983
MalI GATC 5 cut(s) 99, 186, 404, 877, 957
MboI GATC 5 cut(s) 97, 184, 402, 875, 955
MfeI CAATTG 1 cut(s) 188
MflI RGATCY 2 cut(s) 875, 955
MluCI AATT 8 cut(s) 6, 25, 158, 188, 200, 225, 338, 582
MlyI GAGTC 1 cut(s) 34
Mph1103I ATGCAT 1 cut(s) 572
MseI TTAA 4 cut(s) 450, 612, 630, 813
MslI CAYNNNNRTG 2 cut(s) 278, 1048
MspA1I CMGCKG 2 cut(s) 398, 962
MspI CCGG 2 cut(s) 19, 293
MspR9I CCNGG 1 cut(s) 61
MunI CAATTG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 3 cut(s) 404, 647, 894
NdeII GATC 5 cut(s) 97, 184, 402, 875, 955
NheI GCTAGC 1 cut(s) 884
NlaIII CATG 5 cut(s) 197, 277, 351, 574, 1073
NmuCI GTSAC 2 cut(s) 470, 983
NsiI ATGCAT 1 cut(s) 572
NspI RCATGY 3 cut(s) 277, 351, 574
NspV TTCGAA 1 cut(s) 10
OliI CACNNNNGTG 1 cut(s) 1048
PaeI GCATGC 2 cut(s) 277, 574
PasI CCCWGGG 1 cut(s) 60
PceI AGGCCT 1 cut(s) 232
PfeI GAWTC 2 cut(s) 121, 671
PkrI GCNGC 3 cut(s) 67, 211, 896
PleI GAGTC 1 cut(s) 34
PmaCI CACGTG 1 cut(s) 1046
PmlI CACGTG 1 cut(s) 1046
PpsI GAGTC 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 1046
Psp6I CCWGG 1 cut(s) 59
PspCI CACGTG 1 cut(s) 1046
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 59
PstNI CAGNNNCTG 3 cut(s) 482, 641, 656
PsuI RGATCY 2 cut(s) 875, 955
PvuII CAGCTG 2 cut(s) 398, 962
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
RseI CAYNNNNRTG 2 cut(s) 278, 1048
SaqAI TTAA 4 cut(s) 450, 612, 630, 813
SatI GCNGC 3 cut(s) 66, 210, 895
Sau3AI GATC 5 cut(s) 97, 184, 402, 875, 955
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 61
SfaNI GCATC 4 cut(s) 250, 532, 637, 939
SfuI TTCGAA 1 cut(s) 10
SmiMI CAYNNNNRTG 2 cut(s) 278, 1048
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
SphI GCATGC 2 cut(s) 277, 574
Sse9I AATT 8 cut(s) 6, 25, 158, 188, 200, 225, 338, 582
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 2 cut(s) 545, 707
SspMI CTAG 6 cut(s) 431, 524, 818, 879, 885, 891
StuI AGGCCT 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 59
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 2 cut(s) 381, 1048
TaqI TCGA 1 cut(s) 10
TasI AATT 8 cut(s) 6, 25, 158, 188, 200, 225, 338, 582
TatI WGTACW 1 cut(s) 218
TfiI GAWTC 2 cut(s) 121, 671
Tru1I TTAA 4 cut(s) 450, 612, 630, 813
Tru9I TTAA 4 cut(s) 450, 612, 630, 813
TscAI CASTG 1 cut(s) 681
TseFI GTSAC 2 cut(s) 470, 983
TseI GCWGC 3 cut(s) 65, 209, 894
Tsp45I GTSAC 2 cut(s) 470, 983
TspDTI ATGAA 4 cut(s) 213, 762, 819, 915
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 1 cut(s) 681
XapI RAATTY 2 cut(s) 6, 25
XbaI TCTAGA 1 cut(s) 523
XceI RCATGY 3 cut(s) 277, 351, 574
XspI CTAG 6 cut(s) 431, 524, 818, 879, 885, 891
Zsp2I ATGCAT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.