Ubiquitin-conjugating enzyme E2 variant
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom03g10570 pycom11g14450 |
| rosa_multiflora | Rmu_co8203264.1_g000001 |
| rosa_samantha | Rh3BG227700 Rh3DG222900 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 152 |
| AccB1I | GGYRCC | 1 cut(s) | 152 |
| AclWI | GGATC | 2 cut(s) | 15, 28 |
| AcsI | RAATTY | 1 cut(s) | 43 |
| AfaI | GTAC | 2 cut(s) | 97, 154 |
| AluBI | AGCT | 1 cut(s) | 105 |
| AluI | AGCT | 1 cut(s) | 105 |
| AlwI | GGATC | 2 cut(s) | 15, 28 |
| Ama87I | CYCGRG | 1 cut(s) | 65 |
| ApoI | RAATTY | 1 cut(s) | 43 |
| Asp718I | GGTACC | 1 cut(s) | 152 |
| AspLEI | GCGC | 1 cut(s) | 141 |
| AspS9I | GGNCC | 1 cut(s) | 164 |
| AvaI | CYCGRG | 1 cut(s) | 65 |
| AvaII | GGWCC | 1 cut(s) | 164 |
| BamHI | GGATCC | 1 cut(s) | 20 |
| BanI | GGYRCC | 1 cut(s) | 152 |
| BccI | CCATC | 2 cut(s) | 86, 113 |
| Bme18I | GGWCC | 1 cut(s) | 164 |
| BmeT110I | CYCGRG | 1 cut(s) | 65 |
| BmgT120I | GGNCC | 1 cut(s) | 164 |
| BmiI | GGNNCC | 2 cut(s) | 22, 154 |
| BmsI | GCATC | 1 cut(s) | 36 |
| BseGI | GGATG | 1 cut(s) | 121 |
| BseYI | CCCAGC | 1 cut(s) | 199 |
| BshNI | GGYRCC | 1 cut(s) | 152 |
| BsiHKCI | CYCGRG | 1 cut(s) | 65 |
| BsiSI | CCGG | 1 cut(s) | 18 |
| BsoBI | CYCGRG | 1 cut(s) | 65 |
| Bsp143I | GATC | 1 cut(s) | 20 |
| BspLI | GGNNCC | 2 cut(s) | 22, 154 |
| BspPI | GGATC | 2 cut(s) | 15, 28 |
| BspT107I | GGYRCC | 1 cut(s) | 152 |
| BssMI | GATC | 1 cut(s) | 20 |
| Bst4CI | ACNGT | 1 cut(s) | 100 |
| BstF5I | GGATG | 1 cut(s) | 121 |
| BstHHI | GCGC | 1 cut(s) | 141 |
| BstKTI | GATC | 1 cut(s) | 23 |
| BstMBI | GATC | 1 cut(s) | 20 |
| BstNSI | RCATGY | 1 cut(s) | 139 |
| BstX2I | RGATCY | 1 cut(s) | 20 |
| BstYI | RGATCY | 1 cut(s) | 20 |
| BtsCI | GGATG | 1 cut(s) | 121 |
| CfoI | GCGC | 1 cut(s) | 141 |
| Cfr13I | GGNCC | 1 cut(s) | 164 |
| Csp6I | GTAC | 2 cut(s) | 96, 153 |
| CviAII | CATG | 1 cut(s) | 136 |
| CviJI | RGCY | 3 cut(s) | 17, 105, 203 |
| CviKI_1 | RGCY | 3 cut(s) | 17, 105, 203 |
| CviQI | GTAC | 2 cut(s) | 96, 153 |
| DpnI | GATC | 1 cut(s) | 22 |
| DpnII | GATC | 1 cut(s) | 20 |
| Eco47I | GGWCC | 1 cut(s) | 164 |
| Eco88I | CYCGRG | 1 cut(s) | 65 |
| EcoT22I | ATGCAT | 1 cut(s) | 227 |
| FaeI | CATG | 1 cut(s) | 139 |
| FaiI | YATR | 6 cut(s) | 86, 108, 137, 158, 223, 236 |
| FatI | CATG | 1 cut(s) | 135 |
| FokI | GGATG | 1 cut(s) | 128 |
| GlaI | GCGC | 1 cut(s) | 140 |
| GsaI | CCCAGC | 1 cut(s) | 203 |
| HapII | CCGG | 1 cut(s) | 18 |
| HhaI | GCGC | 1 cut(s) | 141 |
| Hin1II | CATG | 1 cut(s) | 139 |
| Hin6I | GCGC | 1 cut(s) | 139 |
| HinP1I | GCGC | 1 cut(s) | 139 |
| HpaII | CCGG | 1 cut(s) | 18 |
| Hpy166II | GTNNAC | 1 cut(s) | 167 |
| Hpy8I | GTNNAC | 1 cut(s) | 167 |
| HpyAV | CCTTC | 1 cut(s) | 73 |
| HpyCH4III | ACNGT | 1 cut(s) | 100 |
| HpyCH4V | TGCA | 1 cut(s) | 225 |
| Hsp92II | CATG | 1 cut(s) | 139 |
| HspAI | GCGC | 1 cut(s) | 139 |
| KpnI | GGTACC | 1 cut(s) | 156 |
| Kzo9I | GATC | 1 cut(s) | 20 |
| LpnPI | CCDG | 5 cut(s) | 31, 37, 41, 213, 221 |
| LweI | GCATC | 1 cut(s) | 36 |
| MalI | GATC | 1 cut(s) | 22 |
| MboI | GATC | 1 cut(s) | 20 |
| MboII | GAAGA | 2 cut(s) | 71, 206 |
| MflI | RGATCY | 1 cut(s) | 20 |
| MluCI | AATT | 2 cut(s) | 43, 159 |
| MnlI | CCTC | 2 cut(s) | 197, 214 |
| Mph1103I | ATGCAT | 1 cut(s) | 227 |
| MslI | CAYNNNNRTG | 2 cut(s) | 32, 134 |
| MspI | CCGG | 1 cut(s) | 18 |
| NdeII | GATC | 1 cut(s) | 20 |
| NlaIII | CATG | 1 cut(s) | 139 |
| NlaIV | GGNNCC | 2 cut(s) | 22, 154 |
| NmeAIII | GCCGAG | 1 cut(s) | 63 |
| NsiI | ATGCAT | 1 cut(s) | 227 |
| NspI | RCATGY | 1 cut(s) | 139 |
| PaeR7I | CTCGAG | 1 cut(s) | 65 |
| PspFI | CCCAGC | 1 cut(s) | 199 |
| PspN4I | GGNNCC | 2 cut(s) | 22, 154 |
| PspPI | GGNCC | 1 cut(s) | 164 |
| PspXI | VCTCGAGB | 1 cut(s) | 65 |
| PsuI | RGATCY | 1 cut(s) | 20 |
| RsaI | GTAC | 2 cut(s) | 97, 154 |
| RsaNI | GTAC | 2 cut(s) | 96, 153 |
| RseI | CAYNNNNRTG | 2 cut(s) | 32, 134 |
| Sau3AI | GATC | 1 cut(s) | 20 |
| Sau96I | GGNCC | 1 cut(s) | 164 |
| SetI | ASST | 2 cut(s) | 53, 107 |
| SfaNI | GCATC | 1 cut(s) | 36 |
| Sfr274I | CTCGAG | 1 cut(s) | 65 |
| SinI | GGWCC | 1 cut(s) | 164 |
| SlaI | CTCGAG | 1 cut(s) | 65 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 32, 134 |
| SmlI | CTYRAG | 1 cut(s) | 65 |
| SmoI | CTYRAG | 1 cut(s) | 65 |
| Sse9I | AATT | 2 cut(s) | 43, 159 |
| TaaI | ACNGT | 1 cut(s) | 100 |
| TaqI | TCGA | 1 cut(s) | 66 |
| TasI | AATT | 2 cut(s) | 43, 159 |
| VpaK11BI | GGWCC | 1 cut(s) | 164 |
| XapI | RAATTY | 1 cut(s) | 43 |
| XceI | RCATGY | 1 cut(s) | 139 |
| XhoI | CTCGAG | 1 cut(s) | 65 |
| Zsp2I | ATGCAT | 1 cut(s) | 227 |