pycom03g23050

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013022
Physical Location & Seq
Forward (+)
23018321 .. 23020631
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23050.8

Sequence Viewer

Length: 1533 bp
ATGGCTCTCTCTTCCACACTTGGACTCCTCCTCCTCCTCTCTTACTCCACCACTCTCCTCCTCCTCTGCAATGCCAACCCCATTCCCGGCCGCCACCGCAGCCCAATCAACCGCCACCCTCGCTCCGCCAGCCACAACTACAGAGATGCCCTCACCAAGTCCATCCTCTTTTTCGAAGGCCAGAGGTCCGGAAAGCTCCCTCCTAACCAGAGGGTTTCCTGGAGGAGAGATTCTGGCCTCTCCGACGGCGCCGCCGCACACGTTGATTTGGTTGGAGGGTACTATGATGCAGGGGACAATGTGAAGTTTGGGTTTCCCATGGCGTTCACCACCACCATGCTTTCATGGAGTGTGATTGAGTTCGGCGGGTTGATGAAGGGTGAGCTGCAGAATGCCCGGCAGGCCATCCGGTGGGCCACCGACTACCTCCTCAAAGCTACTGCGCACCCAGGCACCATTTATGTGCAGGTTGGTGATGCTACAAAGGACCATGCTTGTTGGGAGAGACCAGAAGATATGGACACCCCAAGAACCGTGTTGAAGATTGACCAAAACACCCCTGGTTCTGAAGTTGCAGCTGAAACTGCTGCTGCTCTTGCTTCTGCCTCTTTGGTCTTCAGGAGATGTGATCCCACTTACTCCAAGCTTCTGGTCAGGAGGGCAATGAGCGTGTTTGCGTTTGCCGACAAGCACAGAGGACCTTACAGCATCGGATTGAAGAAATTTGTGTGCCCCTACTATTGTGATTACTCTGGCTTTCAGGATGAGCTGCTGTGGGGTGCTGCTTGGCTGCACAAGGCCACCAAGAATCCGATGTATCTCAGTTACATTCAGGTTAACGGTCAGATTCTCGGGGCGGCGGAGTTTGACAACACATTCGGATGGGATAACAAGCACGTCGGAGCAAGAATTCTTCTCTCCAAGGCATTCCTCGTCCAAAAGGTGAAATCCCTCCATGACTACAAAGGGCATGCAGATAGTTTCATCTGTTCAATCATTCCAGGAGCCTCTTACTCTTCAGCCCAATACACTCCAGGTGGTCTTCTGTTCAAAATGAATGATAGCAACATGCAGTACGTGACATCCACCTCGTTTCTGATCTTAAGCTATGCCAAGTACTTAACCAGTTCCCGCCAGGTGGTTAACTGCGGCGGCACCGCCATCACCCCGAAGCGGCTCCGAGCAATTGCCAAGAGACAGGTGGATTATCTACTAGGAGACAACCCAATGAAGATGTCCTACATGGTGGGGTACGGCCCAAGGTATCCACGGAGGATCGATCACAGGGGCTCATCGCTGCCGTGCATCGCCGCACACCCAGCAAAGATCCAATGCTCGTCCGGCTTCAGCGTGATGAGCTCCCAATCCCCCAACCCCAACATCCTGGTGGGGGCAGTGATCGGTGGGCCCGACAAGAACGACAAGTTCCCAGATCAACGGTCAGATTATGAGCAGTCGGAGCCAGCCACTTACATCAACGCACCCCTCGTAGGATCACTGGCTTATCTTGCCCACTCATTTGGCCAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0007275 GO:0007389 GO:0008150 GO:0008810 GO:0009605 GO:0009607 GO:0009624 GO:0016787 GO:0016798 GO:0032501 GO:0032502 GO:0043207 GO:0048856 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

511

Amino Acids

56.18

Weight (kDa)

9.27

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02800 AT4G02290 AT4G02290
fragaria_vesca FvH4_2g25990 FvH4_2g25990
malus_domestica MD03G1291200.v1.1 MD11G1307100.v1.1
prunus_persica Prupe.8G264200_v2.0.a1
pyrus_communis pycom03g23050 pycom11g26870
rosa_chinensis RchiOBHm_Chr6g0294351
rosa_laevigata RLG00000011878
rosa_multiflora Rmu_sc0005369.1_g000011
rosa_roxburghii Rroxscaffold_7G00173450
rosa_rugosa Rorug06G0243700
rosa_samantha Rh6AG356300 Rh6CG370300
rosa_wichuraiana Rw6G031050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 457
AccB1I GGYRCC 3 cut(s) 248, 452, 1154
AccB7I CCANNNNNTGG 1 cut(s) 411
AccIII TCCGGA 1 cut(s) 188
AclWI GGATC 4 cut(s) 623, 1283, 1321, 1501
AcoI YGGCCR 2 cut(s) 88, 1522
AcsI RAATTY 2 cut(s) 722, 909
AcuI CTGAAG 4 cut(s) 588, 601, 1002, 1330
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 4 cut(s) 281, 1076, 1118, 1253
AfiI CCNNNNNNNGG 6 cut(s) 86, 411, 1138, 1173, 1390, 1490
AflII CTTAAG 1 cut(s) 1102
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 4 cut(s) 541, 718, 993, 1051
AjiI CACGTC 1 cut(s) 898
AjnI CCWGG 7 cut(s) 218, 448, 559, 1000, 1033, 1134, 1383
AluBI AGCT 9 cut(s) 196, 385, 437, 578, 646, 769, 1107, 1359, 1528
AluI AGCT 9 cut(s) 196, 385, 437, 578, 646, 769, 1107, 1359, 1528
Alw21I GWGCWC 1 cut(s) 1361
Alw26I GTCTC 3 cut(s) 499, 1189, 1212
AlwI GGATC 4 cut(s) 623, 1283, 1321, 1501
Ama87I CYCGRG 1 cut(s) 851
Aor13HI TCCGGA 1 cut(s) 188
AoxI GGCC 9 cut(s) 88, 178, 235, 402, 414, 798, 1255, 1406, 1522
ApaI GGGCCC 1 cut(s) 1410
ApeKI GCWGC 9 cut(s) 99, 385, 575, 587, 590, 769, 782, 790, 1297
ApoI RAATTY 2 cut(s) 722, 909
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
AspLEI GCGC 2 cut(s) 251, 445
AspS9I GGNCC 7 cut(s) 186, 414, 487, 698, 1256, 1406, 1407
AsuC2I CCSGG 2 cut(s) 87, 397
AsuHPI GGTGA 6 cut(s) 145, 319, 392, 485, 955, 1156
AsuII TTCGAA 1 cut(s) 174
AvaI CYCGRG 1 cut(s) 851
AvaII GGWCC 3 cut(s) 186, 487, 698
BaeGI GKGCMC 2 cut(s) 734, 1410
BalI TGGCCA 1 cut(s) 1524
BanI GGYRCC 3 cut(s) 248, 452, 1154
BanII GRGCYC 3 cut(s) 1292, 1361, 1410
BarI GAAGNNNNNNTAC 2 cut(s) 1223, 1255
BbsI GAAGAC 2 cut(s) 607, 1034
Bbv12I GWGCWC 1 cut(s) 1361
BbvI GCAGC 9 cut(s) 111, 372, 574, 577, 587, 756, 769, 777, 1284
BccI CCATC 4 cut(s) 170, 413, 876, 1169
BceAI ACGGC 3 cut(s) 262, 1270, 1285
BciT130I CCWGG 7 cut(s) 220, 450, 561, 1002, 1035, 1136, 1385
BciVI GTATCC 1 cut(s) 1275
BcnI CCSGG 2 cut(s) 87, 397
BcoDI GTCTC 3 cut(s) 499, 1189, 1212
BfaI CTAG 1 cut(s) 1214
BfmI CTRYAG 2 cut(s) 139, 386
BfoI RGCGCY 1 cut(s) 252
BfrI CTTAAG 1 cut(s) 1102
BfuAI ACCTGC 1 cut(s) 457
BfuI GTATCC 1 cut(s) 1275
BglI GCCNNNNNGGC 1 cut(s) 401
BmcAI AGTACT 1 cut(s) 1118
Bme1390I CCNGG 9 cut(s) 87, 220, 397, 450, 561, 1002, 1035, 1136, 1385
Bme18I GGWCC 3 cut(s) 186, 487, 698
BmeT110I CYCGRG 1 cut(s) 851
BmgBI CACGTC 1 cut(s) 898
BmgT120I GGNCC 7 cut(s) 186, 414, 487, 698, 1256, 1406, 1407
BmiI GGNNCC 7 cut(s) 250, 454, 1006, 1156, 1178, 1408, 1461
BmrFI CCNGG 9 cut(s) 87, 220, 397, 450, 561, 1002, 1035, 1136, 1385
BmsI GCATC 5 cut(s) 136, 277, 466, 717, 1314
BpiI GAAGAC 2 cut(s) 607, 1034
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 2 cut(s) 241, 1017
Bpu14I TTCGAA 1 cut(s) 174
BpuMI CCSGG 2 cut(s) 87, 397
Bsa29I ATCGAT 1 cut(s) 1278
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 6 cut(s) 318, 448, 559, 921, 1259, 1268
BsaWI WCCGGW 2 cut(s) 188, 408
BsaXI ACNNNNNCTCC 6 cut(s) 9, 15, 39, 45, 107, 137
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 411, 1138, 1173, 1390, 1490
Bse1I ACTGG 2 cut(s) 1125, 1503
Bse3DI GCAATG 2 cut(s) 76, 669
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 7 cut(s) 220, 450, 561, 1002, 1035, 1136, 1385
BseCI ATCGAT 1 cut(s) 1278
BseDI CCNNGG 6 cut(s) 318, 448, 559, 921, 1259, 1268
BseGI GGATG 6 cut(s) 162, 405, 769, 887, 1082, 1380
BseLI CCNNNNNNNGG 6 cut(s) 86, 411, 1138, 1173, 1390, 1490
BseMI GCAATG 2 cut(s) 76, 669
BseMII CTCAG 1 cut(s) 835
BseNI ACTGG 2 cut(s) 1125, 1503
BseRI GAGGAG 9 cut(s) 17, 20, 23, 26, 47, 50, 53, 238, 419
BseSI GKGCMC 2 cut(s) 734, 1410
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 9 cut(s) 111, 372, 574, 577, 587, 756, 769, 777, 1284
BseYI CCCAGC 1 cut(s) 1318
BsgI GTGCAG 2 cut(s) 485, 776
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 9 cut(s) 90, 180, 237, 404, 416, 800, 1257, 1408, 1524
BshNI GGYRCC 3 cut(s) 248, 452, 1154
BshVI ATCGAT 1 cut(s) 1278
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 1361
BsiHKCI CYCGRG 1 cut(s) 851
BsiSI CCGG 5 cut(s) 87, 189, 397, 409, 1341
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 6 cut(s) 86, 411, 1138, 1173, 1390, 1490
BsmAI GTCTC 3 cut(s) 499, 1189, 1212
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 2 cut(s) 397, 926
BsnI GGCC 9 cut(s) 90, 180, 237, 404, 416, 800, 1257, 1408, 1524
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 851
Bsp119I TTCGAA 1 cut(s) 174
Bsp120I GGGCCC 1 cut(s) 1406
Bsp1286I GDGCHC 4 cut(s) 734, 1292, 1361, 1410
Bsp13I TCCGGA 1 cut(s) 188
Bsp143I GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
Bsp19I CCATGG 1 cut(s) 318
BspANI GGCC 9 cut(s) 90, 180, 237, 404, 416, 800, 1257, 1408, 1524
BspCNI CTCAG 1 cut(s) 834
BspDI ATCGAT 1 cut(s) 1278
BspEI TCCGGA 1 cut(s) 188
BspLI GGNNCC 7 cut(s) 250, 454, 1006, 1156, 1178, 1408, 1461
BspMAI CTGCAG 1 cut(s) 390
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 4 cut(s) 623, 1283, 1321, 1501
BspT104I TTCGAA 1 cut(s) 174
BspT107I GGYRCC 3 cut(s) 248, 452, 1154
BspTI CTTAAG 1 cut(s) 1102
BspTNI GGTCTC 1 cut(s) 499
BsrDI GCAATG 2 cut(s) 76, 669
BsrI ACTGG 2 cut(s) 1125, 1503
BssECI CCNNGG 6 cut(s) 318, 448, 559, 921, 1259, 1268
BssMI GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 3 cut(s) 318, 921, 1259
Bst2UI CCWGG 7 cut(s) 220, 450, 561, 1002, 1035, 1136, 1385
Bst4CI ACNGT 3 cut(s) 535, 842, 1440
Bst6I CTCTTC 2 cut(s) 16, 1021
BstACI GRCGYC 1 cut(s) 249
BstAFI CTTAAG 1 cut(s) 1102
BstBAI YACGTR 1 cut(s) 1078
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 5 cut(s) 130, 402, 972, 1464, 1526
BstDEI CTNAG 1 cut(s) 821
BstDSI CCRYGG 2 cut(s) 318, 1268
BstF5I GGATG 6 cut(s) 162, 405, 769, 887, 1082, 1380
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 2 cut(s) 251, 445
BstKTI GATC 8 cut(s) 631, 1101, 1278, 1282, 1329, 1401, 1435, 1496
BstMAI GTCTC 3 cut(s) 499, 1189, 1212
BstMBI GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
BstMCI CGRYCG 1 cut(s) 91
BstNI CCWGG 7 cut(s) 220, 450, 561, 1002, 1035, 1136, 1385
BstNSI RCATGY 2 cut(s) 974, 1072
BstSCI CCNGG 9 cut(s) 85, 218, 395, 448, 559, 1000, 1033, 1134, 1383
BstSFI CTRYAG 2 cut(s) 139, 386
BstSLI GKGCMC 2 cut(s) 734, 1410
BstV1I GCAGC 9 cut(s) 111, 372, 574, 577, 587, 756, 769, 777, 1284
BstV2I GAAGAC 2 cut(s) 607, 1034
BstX2I RGATCY 1 cut(s) 1326
BstXI CCANNNNNNTGG 3 cut(s) 649, 1384, 1520
BstYI RGATCY 1 cut(s) 1326
BstZI CGGCCG 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 1278
BsuI GTATCC 1 cut(s) 1275
BsuRI GGCC 9 cut(s) 90, 180, 237, 404, 416, 800, 1257, 1408, 1524
BsuTUI ATCGAT 1 cut(s) 1278
BtgI CCRYGG 2 cut(s) 318, 1268
BtgZI GCGATG 2 cut(s) 1278, 1291
BtrI CACGTC 1 cut(s) 898
BtsCI GGATG 6 cut(s) 162, 405, 769, 887, 1082, 1380
BtsI GCAGTG 1 cut(s) 1401
BtsIMutI CAGTG 2 cut(s) 1401, 1496
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 5 cut(s) 130, 402, 972, 1464, 1526
CfoI GCGC 2 cut(s) 251, 445
Cfr13I GGNCC 7 cut(s) 186, 414, 487, 698, 1256, 1406, 1407
ClaI ATCGAT 1 cut(s) 1278
Csp6I GTAC 4 cut(s) 280, 1075, 1117, 1252
CviAII CATG 8 cut(s) 319, 337, 345, 491, 956, 971, 1069, 1243
CviQI GTAC 4 cut(s) 280, 1075, 1117, 1252
DdeI CTNAG 1 cut(s) 821
DinI GGCGCC 1 cut(s) 250
DpnI GATC 8 cut(s) 630, 1100, 1277, 1281, 1328, 1400, 1434, 1495
DpnII GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
EaeI YGGCCR 2 cut(s) 88, 1522
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 16, 1021
EarI CTCTTC 2 cut(s) 16, 1021
EciI GGCGGA 2 cut(s) 115, 875
Ecl136II GAGCTC 1 cut(s) 1359
EclXI CGGCCG 1 cut(s) 88
Eco130I CCWWGG 3 cut(s) 318, 921, 1259
Eco24I GRGCYC 3 cut(s) 1292, 1361, 1410
Eco31I GGTCTC 1 cut(s) 499
Eco47I GGWCC 3 cut(s) 186, 487, 698
Eco52I CGGCCG 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 1359
Eco57I CTGAAG 4 cut(s) 588, 601, 1002, 1330
Eco88I CYCGRG 1 cut(s) 851
EcoICRI GAGCTC 1 cut(s) 1359
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 7 cut(s) 218, 448, 559, 1000, 1033, 1134, 1383
EcoT14I CCWWGG 3 cut(s) 318, 921, 1259
EcoT38I GRGCYC 3 cut(s) 1292, 1361, 1410
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
ErhI CCWWGG 3 cut(s) 318, 921, 1259
FaeI CATG 8 cut(s) 322, 340, 348, 494, 959, 974, 1072, 1246
FaqI GGGAC 1 cut(s) 308
FatI CATG 8 cut(s) 318, 336, 344, 490, 955, 970, 1068, 1242
FauI CCCGC 2 cut(s) 359, 1139
FokI GGATG 6 cut(s) 149, 392, 776, 894, 1069, 1367
FriOI GRGCYC 3 cut(s) 1292, 1361, 1410
FspBI CTAG 1 cut(s) 1214
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 2 cut(s) 250, 444
GsaI CCCAGC 1 cut(s) 1322
GsuI CTGGAG 2 cut(s) 241, 1017
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 9 cut(s) 90, 180, 237, 404, 416, 800, 1257, 1408, 1524
HapII CCGG 5 cut(s) 87, 189, 397, 409, 1341
HhaI GCGC 2 cut(s) 251, 445
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 8 cut(s) 322, 340, 348, 494, 959, 974, 1072, 1246
Hin6I GCGC 2 cut(s) 249, 443
HinP1I GCGC 2 cut(s) 249, 443
HincII GTYRAC 2 cut(s) 838, 1144
HindII GTYRAC 2 cut(s) 838, 1144
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 4 cut(s) 24, 230, 808, 847
HpaI GTTAAC 2 cut(s) 838, 1144
HpaII CCGG 5 cut(s) 87, 189, 397, 409, 1341
HphI GGTGA 6 cut(s) 145, 319, 392, 485, 955, 1156
Hpy166II GTNNAC 3 cut(s) 327, 838, 1144
Hpy188III TCNNGA 4 cut(s) 189, 619, 655, 761
Hpy8I GTNNAC 3 cut(s) 327, 838, 1144
Hpy99I CGWCG 2 cut(s) 248, 902
HpyAV CCTTC 2 cut(s) 170, 370
HpyCH4III ACNGT 3 cut(s) 535, 842, 1440
HpyCH4IV ACGT 3 cut(s) 261, 897, 1077
HpyCH4V TGCA 9 cut(s) 69, 290, 388, 466, 575, 793, 974, 1072, 1305
HpyF3I CTNAG 1 cut(s) 821
HpySE526I ACGT 3 cut(s) 261, 897, 1077
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 8 cut(s) 322, 340, 348, 494, 959, 974, 1072, 1246
HspAI GCGC 2 cut(s) 249, 443
KasI GGCGCC 1 cut(s) 248
Kpn2I TCCGGA 1 cut(s) 188
KspAI GTTAAC 2 cut(s) 838, 1144
Kzo9I GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
LmnI GCTCC 7 cut(s) 128, 201, 902, 1004, 1182, 1364, 1459
Lsp1109I GCAGC 9 cut(s) 111, 372, 574, 577, 587, 756, 769, 777, 1284
LweI GCATC 5 cut(s) 136, 277, 466, 717, 1314
MaeI CTAG 1 cut(s) 1214
MaeII ACGT 3 cut(s) 261, 897, 1077
MaeIII GTNAC 2 cut(s) 824, 1078
MalI GATC 8 cut(s) 630, 1100, 1277, 1281, 1328, 1400, 1434, 1495
MboI GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
MboII GAAGA 9 cut(s) 3, 524, 553, 607, 730, 905, 1008, 1034, 1243
MfeI CAATTG 1 cut(s) 1185
MflI RGATCY 1 cut(s) 1326
MhlI GDGCHC 4 cut(s) 734, 1292, 1361, 1410
MlsI TGGCCA 1 cut(s) 1524
MluCI AATT 3 cut(s) 722, 909, 1185
MluNI TGGCCA 1 cut(s) 1524
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 4 cut(s) 253, 267, 880, 1437
Mox20I TGGCCA 1 cut(s) 1524
MroI TCCGGA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 1524
MseI TTAA 4 cut(s) 837, 1103, 1121, 1143
MslI CAYNNNNRTG 4 cut(s) 335, 461, 880, 1385
Msp20I TGGCCA 1 cut(s) 1524
MspA1I CMGCKG 1 cut(s) 578
MspCI CTTAAG 1 cut(s) 1102
MspI CCGG 5 cut(s) 87, 189, 397, 409, 1341
MspR9I CCNGG 9 cut(s) 87, 220, 397, 450, 561, 1002, 1035, 1136, 1385
MunI CAATTG 1 cut(s) 1185
Mva1269I GAATGC 2 cut(s) 397, 926
MvaI CCWGG 7 cut(s) 220, 450, 561, 1002, 1035, 1136, 1385
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 2 cut(s) 87, 397
NcoI CCATGG 1 cut(s) 318
NdeII GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
NlaIII CATG 8 cut(s) 322, 340, 348, 494, 959, 974, 1072, 1246
NlaIV GGNNCC 7 cut(s) 250, 454, 1006, 1156, 1178, 1408, 1461
NmuCI GTSAC 1 cut(s) 1078
NsbI TGCGCA 1 cut(s) 444
NspI RCATGY 2 cut(s) 974, 1072
NspV TTCGAA 1 cut(s) 174
PaeI GCATGC 1 cut(s) 974
PcsI WCGNNNNNNNCGW 2 cut(s) 1407, 1485
PctI GAATGC 2 cut(s) 397, 926
PfeI GAWTC 3 cut(s) 230, 808, 847
PflMI CCANNNNNTGG 1 cut(s) 411
PfoI TCCNGGA 2 cut(s) 218, 1000
PleI GAGTC 1 cut(s) 18
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 18
Ppu21I YACGTR 1 cut(s) 1078
PpuMI RGGWCCY 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 1361
Psp5II RGGWCCY 1 cut(s) 698
Psp6I CCWGG 7 cut(s) 218, 448, 559, 1000, 1033, 1134, 1383
PspFI CCCAGC 1 cut(s) 1318
PspGI CCWGG 7 cut(s) 218, 448, 559, 1000, 1033, 1134, 1383
PspN4I GGNNCC 7 cut(s) 250, 454, 1006, 1156, 1178, 1408, 1461
PspOMI GGGCCC 1 cut(s) 1406
PspPI GGNCC 7 cut(s) 186, 414, 487, 698, 1256, 1406, 1407
PspPPI RGGWCCY 1 cut(s) 698
PstI CTGCAG 1 cut(s) 390
PsuI RGATCY 1 cut(s) 1326
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 4 cut(s) 281, 1076, 1118, 1253
RsaNI GTAC 4 cut(s) 280, 1075, 1117, 1252
RseI CAYNNNNRTG 4 cut(s) 335, 461, 880, 1385
SacI GAGCTC 1 cut(s) 1361
SaqAI TTAA 4 cut(s) 837, 1103, 1121, 1143
Sau3AI GATC 8 cut(s) 628, 1098, 1275, 1279, 1326, 1398, 1432, 1493
Sau96I GGNCC 7 cut(s) 186, 414, 487, 698, 1256, 1406, 1407
ScaI AGTACT 1 cut(s) 1118
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 9 cut(s) 87, 220, 397, 450, 561, 1002, 1035, 1136, 1385
SduI GDGCHC 4 cut(s) 734, 1292, 1361, 1410
SfaNI GCATC 5 cut(s) 136, 277, 466, 717, 1314
SfcI CTRYAG 2 cut(s) 139, 386
SfoI GGCGCC 1 cut(s) 250
SfuI TTCGAA 1 cut(s) 174
SinI GGWCC 3 cut(s) 186, 487, 698
SmiMI CAYNNNNRTG 4 cut(s) 335, 461, 880, 1385
SmlI CTYRAG 1 cut(s) 1102
SmoI CTYRAG 1 cut(s) 1102
SphI GCATGC 1 cut(s) 974
Sse9I AATT 3 cut(s) 722, 909, 1185
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 1 cut(s) 1214
SstI GAGCTC 1 cut(s) 1361
StyD4I CCNGG 9 cut(s) 85, 218, 395, 448, 559, 1000, 1033, 1134, 1383
StyI CCWWGG 3 cut(s) 318, 921, 1259
TaaI ACNGT 3 cut(s) 535, 842, 1440
TaiI ACGT 3 cut(s) 264, 900, 1080
TaqI TCGA 2 cut(s) 174, 1278
TasI AATT 3 cut(s) 722, 909, 1185
TatI WGTACW 1 cut(s) 1116
TauI GCSGC 8 cut(s) 93, 254, 257, 860, 1152, 1155, 1177, 1313
TfiI GAWTC 3 cut(s) 230, 808, 847
Tru1I TTAA 4 cut(s) 837, 1103, 1121, 1143
Tru9I TTAA 4 cut(s) 837, 1103, 1121, 1143
TscAI CASTG 2 cut(s) 1401, 1503
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 9 cut(s) 99, 385, 575, 587, 590, 769, 782, 790, 1297
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 5 cut(s) 333, 389, 973, 1070, 1244
TspGWI ACGGA 1 cut(s) 1285
TspRI CASTG 2 cut(s) 1401, 1503
Van91I CCANNNNNTGG 1 cut(s) 411
Vha464I CTTAAG 1 cut(s) 1102
VpaK11BI GGWCC 3 cut(s) 186, 487, 698
XapI RAATTY 2 cut(s) 722, 909
XceI RCATGY 2 cut(s) 974, 1072
XcmI CCANNNNNNNNNTGG 3 cut(s) 557, 1198, 1384
XspI CTAG 1 cut(s) 1214
ZrmI AGTACT 1 cut(s) 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.