pycom04g03880

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0018331
Physical Location & Seq
Reverse (-)
3481959 .. 3487988
6030 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1770 bp
ATGGCGATGGACGATCGGCTGGCGGAGCTCAATCCAAAGCCCAGAACCTGCGTCGTCTTAGGCGGCCGAGGATTCCTCGGGCGATCGCTCGTCCTCCGGCTCTTGAATATCGGAAAGTGGATCGTTCGCGTTGCCGATTCGACTCCGTCCCTCCAGCTCGATCCCTCCGAGCGCGACTCCCTACTCTCGCAAGCTCTCTCCTCCGGCCGCGCTTCCTTACACGGCGTCGATGTCCGCGACACGTCTCAAATCATCACTGCCATTGAAGGTTCGTCTGTAGTGTTCTATTTGGATGGAGATGATTTACCCACACATGATTTCTACCAGTGCTATATGATTGTTGTTCAGGGTGCAAAAAATGTCATCAGTGCTTGTCGAGAGTGCAAAGTTAGGCGGTTAATATACAATAGTTCTGCTGATGTAGTTTTTGATGGATCAAAAGATATATTAAATGGAGACGAATCTTTGGCATACCCTTGGAAATTTGATGATATATTGAGTGACCTCAAGGCTCAAGCAGAATCACTTATCCTGCTTTCTAATGACATTGATGCTCTTTTAACATGTGTGCTTCGTCCTAGCAATGTATTTGGACCTGGAGACACACATCTTGTGCCATTTTTAGTGAATTTGGCAAAATCTGGCTGGGGAAAGTTTATTATAGGGAGTGGTGAAAACATGTCAGATTTCACATACGTTGAGAATGTTACTCATGCTCATATCTGTGCAGAAGAAGCTTTAGAATTTCGGATGGTCTCTGCTGCTGGAAAGGCCTTTTTCATCACAAATCTCAAGCCTGCAAATTTTTGGGTATTTGTATTACTAATCTTGGAATGCCTGGGATGTCAAAGGCCATTACTGAAAGTTCCTGCTAAGATGGCTTGGTATATTCTTTTGTTCATCAAACGGATAGCCGAAAAGTATGGAAGGATAAAATACAACGATTCTATGTCAGCGCATTACATTCAATTAGCTTCACGTAGTAGAACGTTCAACTGCACTGCGGCTCAAAACCAGATTGGGTATTCACCAGTTGTCTCACTGGAAGAAGGTGTCAAATTAACCGTTGAGTCATTCTCTAATATAGCCAGAAACTCACCCATCCCAACTTTCAGAAACTTTAATGAGGAATCAAAAGTGGAGAAGCTGCTAGGCAGTGGAGAAGTTGCAGACATTCTGCTATGGCGTGATGAGAAGAAGTCTTTTACATATTTCCTTGCTTTGAGTATGTTGTACTATTGGTTTTTCCTTTCCGGAAGAACTTTTGCCTCGTCTATGTCCAAGCTTCTGCTGTTCGTTTCATTTGGCCTCTATGGATACGGGATTCTACCACCCAAGATGTTGGGTTTTACCTTTAAGACACTATCTTTATCTTGTTTTGAGATCCCTGAAATGGTAGTGAAAGAGAAGATTGCAACGATAGCATATCTGTGGAACAGAGGGGTTTGCTATTTGAGACTACTGGCCCAGGGAGATGGGTGGGATATTTTCTTCAAGTTTGCAGCTTCCCTCGTTTTCCTGAAGTTGATTTTATCTCATGCTTCGACTGTGTCGTTAGGTGTAGCTCTGGTGTTGGCTTTCACGCTATTTTTCGTTTGTGAGCAATATGAATCGGAAATAGAAGGATTGGCAAAGCTACTGTTCAACAGGTTCATATCAGTTTCTGGGTCGTTGAAAACAAATGTACCCGCTTCTGTGCAACAATATCTTCAAAAACGTGGAATCTTGCATCACGATAAAGGAGCTGCAACAGTAAAGCATCAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01130 map00100 map01100 map01130
Pfam Domains
Protein Families
3Beta_HSD Reticulon

Protein Analysis

590

Amino Acids

65.78

Weight (kDa)

8.44

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 18 - 285 2.5e-72 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 18 - 246 1.6e-21 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 19 - 273 6.8e-07 GDP-mannose 4,6 dehydratase
Reticulon PF02453 389 - 545 3.5e-25 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 56
AccII CGCG 4 cut(s) 129, 174, 210, 237
AccIII TCCGGA 1 cut(s) 1253
AciI CCGC 7 cut(s) 23, 63, 208, 235, 394, 1004, 1689
AclI AACGTT 1 cut(s) 989
AclWI GGATC 4 cut(s) 128, 155, 442, 1378
AcoI YGGCCR 2 cut(s) 64, 205
AcsI RAATTY 4 cut(s) 482, 628, 743, 802
AcuI CTGAAG 1 cut(s) 1541
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 2 cut(s) 1235, 1686
AfiI CCNNNNNNNGG 1 cut(s) 1393
AflIII ACRYGT 3 cut(s) 240, 563, 678
AgsI TTSAA 8 cut(s) 106, 266, 968, 994, 1495, 1645, 1675, 1712
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 3 cut(s) 595, 837, 1467
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 6 cut(s) 249, 450, 594, 760, 1042, 1450
AlwI GGATC 4 cut(s) 128, 155, 442, 1378
AlwNI CAGNNNCTG 2 cut(s) 48, 1664
Ama87I CYCGRG 1 cut(s) 77
Aor13HI TCCGGA 1 cut(s) 1253
AoxI GGCC 6 cut(s) 64, 205, 771, 851, 1306, 1464
ApeKI GCWGC 4 cut(s) 761, 1147, 1502, 1745
ApoI RAATTY 4 cut(s) 482, 628, 743, 802
AsiSI GCGATCGC 1 cut(s) 86
AspLEI GCGC 3 cut(s) 174, 212, 958
AspS9I GGNCC 2 cut(s) 593, 1465
AsuHPI GGTGA 3 cut(s) 683, 1020, 1089
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 1 cut(s) 593
BaeI ACNNNNGTAYC 2 cut(s) 1668, 1701
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 4 cut(s) 748, 1134, 1514, 1732
BccI CCATC 6 cut(s) 287, 425, 745, 871, 1109, 1469
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 3 cut(s) 597, 839, 1469
BciVI GTATCC 1 cut(s) 1310
BcoDI GTCTC 6 cut(s) 249, 450, 594, 760, 1042, 1450
BfaI CTAG 2 cut(s) 579, 1151
BfmI CTRYAG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 56
BfuI GTATCC 1 cut(s) 1310
BisI GCNGC 7 cut(s) 64, 208, 762, 1005, 1148, 1503, 1746
BlsI GCNGC 7 cut(s) 65, 209, 763, 1006, 1149, 1504, 1747
Bme1390I CCNGG 3 cut(s) 597, 839, 1469
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 77
BmgBI CACGTC 1 cut(s) 243
BmgT120I GGNCC 2 cut(s) 593, 1465
BmrFI CCNGG 3 cut(s) 597, 839, 1469
BmsI GCATC 2 cut(s) 541, 1738
BplI GAGNNNNNCTC 6 cut(s) 60, 92, 161, 193, 1061, 1093
BpmI CTGGAG 2 cut(s) 137, 618
BpuEI CTTGAG 3 cut(s) 491, 498, 776
BsaAI YACGTR 1 cut(s) 980
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 760
BsaJI CCNNGG 6 cut(s) 67, 76, 476, 838, 1467, 1468
BsaWI WCCGGW 1 cut(s) 1253
Bsc4I CCNNNNNNNGG 1 cut(s) 1393
Bse1I ACTGG 4 cut(s) 325, 1031, 1047, 1467
Bse3DI GCAATG 1 cut(s) 589
BseAI TCCGGA 1 cut(s) 1253
BseBI CCWGG 3 cut(s) 597, 839, 1469
BseDI CCNNGG 6 cut(s) 67, 76, 476, 838, 1467, 1468
BseGI GGATG 4 cut(s) 298, 756, 848, 1101
BseLI CCNNNNNNNGG 1 cut(s) 1393
BseMI GCAATG 1 cut(s) 589
BseNI ACTGG 4 cut(s) 325, 1031, 1047, 1467
BseRI GAGGAG 1 cut(s) 190
BseX3I CGGCCG 2 cut(s) 64, 205
BseXI GCAGC 4 cut(s) 748, 1134, 1514, 1732
BseYI CCCAGC 1 cut(s) 645
BsgI GTGCAG 2 cut(s) 747, 982
Bsh1236I CGCG 4 cut(s) 129, 174, 210, 237
Bsh1285I CGRYCG 4 cut(s) 16, 67, 86, 208
BshFI GGCC 6 cut(s) 66, 207, 773, 853, 1308, 1466
BsiEI CGRYCG 4 cut(s) 16, 67, 86, 208
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 3 cut(s) 97, 204, 1254
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 1393
BsmAI GTCTC 6 cut(s) 249, 450, 594, 760, 1042, 1450
BsmBI CGTCTC 2 cut(s) 249, 450
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 6 cut(s) 66, 207, 773, 853, 1308, 1466
Bso31I GGTCTC 1 cut(s) 760
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 30
Bsp13I TCCGGA 1 cut(s) 1253
Bsp143I GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
BspACI CCGC 7 cut(s) 23, 63, 208, 235, 394, 1004, 1689
BspANI GGCC 6 cut(s) 66, 207, 773, 853, 1308, 1466
BspEI TCCGGA 1 cut(s) 1253
BspFNI CGCG 4 cut(s) 129, 174, 210, 237
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 4 cut(s) 128, 155, 442, 1378
BspTNI GGTCTC 1 cut(s) 760
BsrDI GCAATG 1 cut(s) 589
BsrI ACTGG 4 cut(s) 325, 1031, 1047, 1467
BssECI CCNNGG 6 cut(s) 67, 76, 476, 838, 1467, 1468
BssMI GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 3 cut(s) 597, 839, 1469
Bst4CI ACNGT 4 cut(s) 1066, 1549, 1641, 1753
BstACI GRCGYC 1 cut(s) 225
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 3 cut(s) 21, 192, 798
BstDEI CTNAG 2 cut(s) 58, 873
BstF5I GGATG 4 cut(s) 298, 756, 848, 1101
BstFNI CGCG 4 cut(s) 129, 174, 210, 237
BstHHI GCGC 3 cut(s) 174, 212, 958
BstKTI GATC 6 cut(s) 16, 86, 123, 163, 437, 1386
BstMAI GTCTC 6 cut(s) 249, 450, 594, 760, 1042, 1450
BstMBI GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
BstMCI CGRYCG 4 cut(s) 16, 67, 86, 208
BstMWI GCNNNNNNNGC 5 cut(s) 25, 734, 770, 878, 1421
BstNI CCWGG 3 cut(s) 597, 839, 1469
BstNSI RCATGY 2 cut(s) 567, 682
BstSCI CCNGG 3 cut(s) 595, 837, 1467
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 4 cut(s) 129, 174, 210, 237
BstV1I GCAGC 4 cut(s) 748, 1134, 1514, 1732
BstX2I RGATCY 1 cut(s) 1383
BstXI CCANNNNNNTGG 2 cut(s) 1342, 1475
BstYI RGATCY 1 cut(s) 1383
BstZI CGGCCG 2 cut(s) 64, 205
BsuI GTATCC 1 cut(s) 1310
BsuRI GGCC 6 cut(s) 66, 207, 773, 853, 1308, 1466
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 4 cut(s) 298, 756, 848, 1101
BtsI GCAGTG 3 cut(s) 255, 999, 1162
BtsIMutI CAGTG 6 cut(s) 255, 332, 373, 999, 1040, 1162
BveI ACCTGC 1 cut(s) 56
Cac8I GCNNGC 3 cut(s) 21, 192, 798
CaiI CAGNNNCTG 2 cut(s) 48, 1664
CfoI GCGC 3 cut(s) 174, 212, 958
Cfr13I GGNCC 2 cut(s) 593, 1465
CseI GACGC 2 cut(s) 40, 214
Csp6I GTAC 2 cut(s) 1234, 1685
CviAII CATG 5 cut(s) 314, 564, 679, 713, 1538
CviQI GTAC 2 cut(s) 1234, 1685
DdeI CTNAG 2 cut(s) 58, 873
DpnI GATC 6 cut(s) 15, 85, 122, 162, 436, 1385
DpnII GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
EaeI YGGCCR 2 cut(s) 64, 205
EagI CGGCCG 2 cut(s) 64, 205
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 28
EclXI CGGCCG 2 cut(s) 64, 205
Eco130I CCWWGG 1 cut(s) 476
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 760
Eco47I GGWCC 1 cut(s) 593
Eco52I CGGCCG 2 cut(s) 64, 205
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 1541
Eco88I CYCGRG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 28
EcoRII CCWGG 3 cut(s) 595, 837, 1467
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 476
Esp3I CGTCTC 2 cut(s) 249, 450
FaeI CATG 5 cut(s) 317, 567, 682, 716, 1541
FaqI GGGAC 1 cut(s) 133
FatI CATG 5 cut(s) 313, 563, 678, 712, 1537
FauI CCCGC 1 cut(s) 1696
Fnu4HI GCNGC 7 cut(s) 64, 208, 762, 1005, 1148, 1503, 1746
FokI GGATG 4 cut(s) 305, 763, 855, 1088
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 7 cut(s) 64, 208, 762, 1005, 1148, 1503, 1746
FspBI CTAG 2 cut(s) 579, 1151
GlaI GCGC 3 cut(s) 173, 211, 957
GluI GCNGC 7 cut(s) 64, 208, 762, 1005, 1148, 1503, 1746
GsaI CCCAGC 1 cut(s) 649
GsuI CTGGAG 2 cut(s) 137, 618
HaeIII GGCC 6 cut(s) 66, 207, 773, 853, 1308, 1466
HapII CCGG 3 cut(s) 97, 204, 1254
HgaI GACGC 2 cut(s) 40, 214
HhaI GCGC 3 cut(s) 174, 212, 958
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 5 cut(s) 317, 567, 682, 716, 1541
Hin6I GCGC 3 cut(s) 172, 210, 956
HinP1I GCGC 3 cut(s) 172, 210, 956
HindIII AAGCTT 2 cut(s) 735, 1283
HpaII CCGG 3 cut(s) 97, 204, 1254
HphI GGTGA 3 cut(s) 683, 1020, 1089
Hpy188I TCNGA 7 cut(s) 113, 169, 685, 750, 1115, 1615, 1764
Hpy188III TCNNGA 5 cut(s) 103, 377, 1254, 1519, 1733
Hpy99I CGWCG 2 cut(s) 56, 230
HpyAV CCTTC 4 cut(s) 260, 921, 1043, 1616
HpyCH4III ACNGT 4 cut(s) 1066, 1549, 1641, 1753
HpyCH4IV ACGT 5 cut(s) 242, 696, 979, 989, 1717
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 734, 770, 878, 1421
HpyF3I CTNAG 2 cut(s) 58, 873
HpySE526I ACGT 5 cut(s) 242, 696, 979, 989, 1717
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 5 cut(s) 317, 567, 682, 716, 1541
HspAI GCGC 3 cut(s) 172, 210, 956
Kpn2I TCCGGA 1 cut(s) 1253
Kzo9I GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
LmnI GCTCC 2 cut(s) 25, 1742
Lsp1109I GCAGC 4 cut(s) 748, 1134, 1514, 1732
LweI GCATC 2 cut(s) 541, 1738
MaeI CTAG 2 cut(s) 579, 1151
MaeII ACGT 5 cut(s) 242, 696, 979, 989, 1717
MaeIII GTNAC 2 cut(s) 500, 706
MalI GATC 6 cut(s) 15, 85, 122, 162, 436, 1385
MboI GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
MboII GAAGA 7 cut(s) 743, 1058, 1207, 1269, 1420, 1483, 1700
MflI RGATCY 1 cut(s) 1383
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 6 cut(s) 482, 628, 743, 802, 968, 1058
MlyI GAGTC 3 cut(s) 136, 170, 1079
MroI TCCGGA 1 cut(s) 1253
MseI TTAA 6 cut(s) 398, 449, 560, 1061, 1122, 1356
MslI CAYNNNNRTG 2 cut(s) 723, 1429
MspI CCGG 3 cut(s) 97, 204, 1254
MspR9I CCNGG 3 cut(s) 597, 839, 1469
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 3 cut(s) 597, 839, 1469
MvnI CGCG 4 cut(s) 129, 174, 210, 237
MwoI GCNNNNNNNGC 5 cut(s) 25, 734, 770, 878, 1421
NdeII GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
NlaIII CATG 5 cut(s) 317, 567, 682, 716, 1541
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 2 cut(s) 567, 682
PasI CCCWGGG 1 cut(s) 1468
PceI AGGCCT 1 cut(s) 773
PciI ACATGT 2 cut(s) 563, 678
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 234, 1550
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 9 cut(s) 72, 137, 461, 521, 944, 1130, 1324, 1610, 1722
PflFI GACNNNGTC 2 cut(s) 145, 1549
PkrI GCNGC 7 cut(s) 65, 209, 763, 1006, 1149, 1504, 1747
Ple19I CGATCG 2 cut(s) 16, 86
PleI GAGTC 3 cut(s) 136, 170, 1078
PpsI GAGTC 3 cut(s) 136, 170, 1078
Ppu21I YACGTR 1 cut(s) 980
PscI ACATGT 2 cut(s) 563, 678
Psp124BI GAGCTC 1 cut(s) 30
Psp1406I AACGTT 1 cut(s) 989
Psp6I CCWGG 3 cut(s) 595, 837, 1467
PspFI CCCAGC 1 cut(s) 645
PspGI CCWGG 3 cut(s) 595, 837, 1467
PspPI GGNCC 2 cut(s) 593, 1465
PstNI CAGNNNCTG 2 cut(s) 48, 1664
PsuI RGATCY 1 cut(s) 1383
PsyI GACNNNGTC 2 cut(s) 145, 1549
PvuI CGATCG 2 cut(s) 16, 86
RgaI GCGATCGC 1 cut(s) 86
RsaI GTAC 2 cut(s) 1235, 1686
RsaNI GTAC 2 cut(s) 1234, 1685
RseI CAYNNNNRTG 2 cut(s) 723, 1429
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 6 cut(s) 398, 449, 560, 1061, 1122, 1356
SatI GCNGC 7 cut(s) 64, 208, 762, 1005, 1148, 1503, 1746
Sau3AI GATC 6 cut(s) 13, 83, 120, 160, 434, 1383
Sau96I GGNCC 2 cut(s) 593, 1465
SchI GAGTC 3 cut(s) 136, 170, 1079
ScrFI CCNGG 3 cut(s) 597, 839, 1469
SduI GDGCHC 1 cut(s) 30
SfaAI GCGATCGC 1 cut(s) 86
SfaNI GCATC 2 cut(s) 541, 1738
SfcI CTRYAG 1 cut(s) 276
SgfI GCGATCGC 1 cut(s) 86
SinI GGWCC 1 cut(s) 593
SmiMI CAYNNNNRTG 2 cut(s) 723, 1429
SmlI CTYRAG 3 cut(s) 506, 513, 791
SmoI CTYRAG 3 cut(s) 506, 513, 791
Sse9I AATT 6 cut(s) 482, 628, 743, 802, 968, 1058
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 7 cut(s) 23, 63, 208, 235, 394, 1004, 1689
SspMI CTAG 2 cut(s) 579, 1151
SstI GAGCTC 1 cut(s) 30
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 3 cut(s) 595, 837, 1467
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 4 cut(s) 1066, 1549, 1641, 1753
TaiI ACGT 5 cut(s) 245, 699, 982, 992, 1720
TaqI TCGA 5 cut(s) 140, 159, 228, 376, 1544
TasI AATT 6 cut(s) 482, 628, 743, 802, 968, 1058
TatI WGTACW 1 cut(s) 1233
TauI GCSGC 3 cut(s) 66, 210, 1007
TfiI GAWTC 9 cut(s) 72, 137, 461, 521, 944, 1130, 1324, 1610, 1722
Tru1I TTAA 6 cut(s) 398, 449, 560, 1061, 1122, 1356
Tru9I TTAA 6 cut(s) 398, 449, 560, 1061, 1122, 1356
TscAI CASTG 6 cut(s) 262, 332, 373, 1006, 1047, 1162
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 4 cut(s) 761, 1147, 1502, 1745
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 5 cut(s) 769, 889, 1290, 1623, 1642
TspGWI ACGGA 2 cut(s) 135, 922
TspRI CASTG 6 cut(s) 262, 332, 373, 1006, 1047, 1162
Tth111I GACNNNGTC 2 cut(s) 145, 1549
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 4 cut(s) 482, 628, 743, 802
XceI RCATGY 2 cut(s) 567, 682
XspI CTAG 2 cut(s) 579, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.