pycom04g04380

Phospholipase A1-Ibeta2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014430
Physical Location & Seq
Forward (+)
3964236 .. 3965867
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04380.1

Sequence Viewer

Length: 1632 bp
ATGATGCTCGTCGGACCAACGCTCCCAGCTCACAACGTCAACCTGTTTCAGGCCAGAAGCGCCAGCTTCAGAAACCACACGTCCCCATTAAACCCATCAACGATAGCTCCATCCTCTTCTTCTTATTCACCCGCCTCCATTAAACCCGGTTCTAACTCCACCAAGATGACTCGGCTCCACCTCGCCAACCTCGACAAGCTCCTCCAAACCCCACCTCCGCCCCAAACCCAACTCGACCCACAACCCGATCTCGAAAAGGACAAAGATCCGCATAAAAATGGGTCACGAGAAAACAGGGGAAGGGGGCTTCTGGGAGGGCTCAAACTTTCTCGGTTATGGACGCAAGTGAAAGGCGCCGCCGAAGACATGTCTCCGAGACACCTCAAGCGCCTGCAGCGCCTCCTGTCCAAGACTGGAGAGTACTCTCCCAGAAATAATTTGGGCACCCGGTGGCGCGAGTTTCACGGTTCGAACAACTGGGCCGGACTACTTGACCCACTGGATGAGAATCTCCGGCGAGAGGTCGTCAGATACGGCGAGTTTATTCAGGCGGCTTATCAATCGTTTCATTCGAATCCAGCCATGTCGGCGGAGGAGGTTCAGATGCCTCGCAACGTGGCGCTTCCGGATAGATCATATAAGGTAACGAAGAGCCTTTACGCCACGTCATCAATCGGGTTGCCCGATTGGGTCGATGAAGTGAGGCCGGATCTCAGTTGGATGACCCAGCGGTCGAGCTGGGTCGGGTACGTGGCAGTTTGTGATGATAAGGGGGAGATTGCACGGATGGGCAGACGCGATATTGTCATTGCCCTCCGTGGGACCTCCACGTGTCTTGAATGGGCCGAGAATTTAAGAGCTCAACTAATTCAGATGCCGTCGACCGAGCAAGGTCAACAGGAACGCCAAGCAAAAGTGGAATGCGGATTTTTGAGTCTCTACAAAACACGCGGGGCCGACGTGCCGAGCCTTGCCGAGTCAGTGGTTGAAGAGGTCAAGAGGCTGATAGAGCAATACAAGGGAGAAACATTAAGCATAACTGTGACAGGACACAGCCTTGGTGCTGCCTTAGCCTTGTTAGTAGGCGATGAGCTCAAAACATGCGCCGAGAATATGCCGTCGGTGGCGGTTTTCTCCTTTGGAGGCCCGCGCGTAGGTAACCGAGGATTCACAAATCGAATGGACGACAAGGATGTGAAGGTATTGAGGATCGTCAACTCTCAAGACGTGATCACTAGGGTTCCCGGTATTTTCGTGGGCGAAGGAGCGCTCGAGGAGAAGTTGAAGAACGCAAAGGCCGGAGGATTCATCGACATGATCGACAGGAACATGCCGTTGGCTTACACACATGTTGGGACAGAATTGAGAGTCGACACAAAAATGTCACCTTATTTGAAGCCCGATGCAGATGTGGCTTGCTGCCATGATTTGGAGGCATATTTGCATTTGGTGGATGGGTTTTTGGCATCTAATTGTCCTTTTAGAGAAAATGCTAAAAGGAGCTTAGTGAGGTTGCTTCAGGATCAAAGATCTAATGTGAAAAAATTGTATACAAGCAAGGCAAATAATTTGACTAGTTTAAAACTGGAAAGAACGGGACTGATGGTTAGTTGTTTACCTAGTCCATCATAA

Protein Analysis

544

Amino Acids

60.28

Weight (kDa)

9.08

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 269 - 417 3.5e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16820
fragaria_vesca FvH4_5g19980
malus_domestica MD04G1049800.v1.1 MD06G1050100.v1.1
prunus_persica Prupe.5G060400_v2.0.a1
pyrus_communis pycom04g04380 pycom06g04250
rosa_chinensis RchiOBHm_Chr7g0204811
rosa_laevigata RLG00000003431
rosa_multiflora Rmu_sc0003221.1_g000039
rosa_roxburghii Rroxscaffold_3G00252770
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216100 Rh7BG213200 Rh7CG229400 Rh7DG223800
rosa_wichuraiana Rw7G018740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 730
AccB1I GGYRCC 2 cut(s) 353, 443
AccB7I CCANNNNNTGG 1 cut(s) 1429
AccI GTMKAC 3 cut(s) 881, 1371, 1550
AccII CGCG 5 cut(s) 456, 798, 951, 1150, 1152
AccIII TCCGGA 1 cut(s) 625
AclWI GGATC 4 cut(s) 260, 717, 1217, 1530
AcsI RAATTY 1 cut(s) 850
AcuI CTGAAG 2 cut(s) 52, 1502
AcvI CACGTG 1 cut(s) 831
AcyI GRCGYC 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 450
AfaI GTAC 2 cut(s) 422, 749
AfeI AGCGCT 1 cut(s) 1269
AfiI CCNNNNNNNGG 5 cut(s) 49, 520, 819, 1154, 1429
AflIII ACRYGT 4 cut(s) 78, 366, 830, 1348
AgsI TTSAA 4 cut(s) 839, 989, 1285, 1396
AhlI ACTAGT 1 cut(s) 1574
AjiI CACGTC 4 cut(s) 81, 666, 961, 1228
AjuI GAANNNNNNNTTGG 2 cut(s) 1319, 1351
AluBI AGCT 8 cut(s) 29, 66, 107, 199, 738, 860, 1093, 1503
AluI AGCT 8 cut(s) 29, 66, 107, 199, 738, 860, 1093, 1503
Alw21I GWGCWC 2 cut(s) 862, 1095
Alw26I GTCTC 3 cut(s) 370, 375, 941
AlwI GGATC 4 cut(s) 260, 717, 1217, 1530
Ama87I CYCGRG 1 cut(s) 1271
Aor13HI TCCGGA 1 cut(s) 625
Aor51HI AGCGCT 1 cut(s) 1269
AoxI GGCC 7 cut(s) 51, 480, 704, 843, 954, 1144, 1296
ApeKI GCWGC 3 cut(s) 394, 1064, 1419
ApoI RAATTY 1 cut(s) 850
AspLEI GCGC 9 cut(s) 62, 356, 390, 399, 456, 622, 1106, 1152, 1270
AspS9I GGNCC 6 cut(s) 14, 480, 822, 843, 954, 1145
AsuC2I CCSGG 3 cut(s) 147, 448, 1245
AsuHPI GGTGA 2 cut(s) 120, 1377
AsuII TTCGAA 2 cut(s) 470, 572
AvaI CYCGRG 1 cut(s) 1271
AvaII GGWCC 2 cut(s) 14, 822
BaeGI GKGCMC 1 cut(s) 446
BanI GGYRCC 2 cut(s) 353, 443
BanII GRGCYC 3 cut(s) 321, 862, 1095
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BauI CACGAG 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 2 cut(s) 862, 1095
BbvI GCAGC 3 cut(s) 406, 1051, 1406
BccI CCATC 5 cut(s) 103, 118, 781, 1448, 1597
BceAI ACGGC 4 cut(s) 550, 862, 1102, 1318
BclI TGATCA 1 cut(s) 1230
BcnI CCSGG 3 cut(s) 147, 448, 1245
BcoDI GTCTC 3 cut(s) 370, 375, 941
BcuI ACTAGT 1 cut(s) 1574
BfaI CTAG 3 cut(s) 1236, 1575, 1620
BfmI CTRYAG 1 cut(s) 392
BfoI RGCGCY 6 cut(s) 63, 357, 391, 400, 623, 1271
BglI GCCNNNNNGGC 1 cut(s) 587
BglII AGATCT 1 cut(s) 1529
BisI GCNGC 5 cut(s) 357, 395, 552, 1065, 1420
BlsI GCNGC 5 cut(s) 358, 396, 553, 1066, 1421
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 3 cut(s) 147, 448, 1245
Bme18I GGWCC 2 cut(s) 14, 822
BmeT110I CYCGRG 1 cut(s) 1271
BmgBI CACGTC 4 cut(s) 81, 666, 961, 1228
BmgT120I GGNCC 6 cut(s) 14, 480, 822, 843, 954, 1145
BmiI GGNNCC 6 cut(s) 176, 355, 445, 823, 955, 1242
BmrFI CCNGG 3 cut(s) 147, 448, 1245
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 4 cut(s) 594, 864, 1393, 1475
BmuI ACTGGG 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 1069
Bpu14I TTCGAA 2 cut(s) 470, 572
BpuEI CTTGAG 2 cut(s) 368, 1206
BpuMI CCSGG 3 cut(s) 147, 448, 1245
BsaAI YACGTR 2 cut(s) 751, 831
BsaBI GATNNNNATC 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 817, 1057, 1162
BsaWI WCCGGW 1 cut(s) 625
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 91, 121, 199, 229
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 520, 819, 1154, 1429
Bse1I ACTGG 4 cut(s) 418, 482, 504, 1590
Bse3DI GCAATG 1 cut(s) 807
Bse8I GATNNNNATC 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 625
BseDI CCNNGG 3 cut(s) 817, 1057, 1162
BseGI GGATG 6 cut(s) 110, 508, 726, 792, 1198, 1459
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 5 cut(s) 49, 520, 819, 1154, 1429
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 1 cut(s) 727
BseNI ACTGG 4 cut(s) 418, 482, 504, 1590
BseRI GAGGAG 3 cut(s) 191, 608, 1289
BseSI GKGCMC 1 cut(s) 446
BseXI GCAGC 3 cut(s) 406, 1051, 1406
BseYI CCCAGC 3 cut(s) 25, 726, 738
Bsh1236I CGCG 5 cut(s) 456, 798, 951, 1150, 1152
Bsh1285I CGRYCG 2 cut(s) 734, 885
BshFI GGCC 7 cut(s) 53, 482, 706, 845, 956, 1146, 1298
BshNI GGYRCC 2 cut(s) 353, 443
BsiEI CGRYCG 2 cut(s) 734, 885
BsiHKAI GWGCWC 2 cut(s) 862, 1095
BsiHKCI CYCGRG 1 cut(s) 1271
BsiSI CCGG 8 cut(s) 147, 448, 483, 514, 626, 707, 1245, 1299
BslFI GGGAC 4 cut(s) 67, 835, 1369, 1611
BslI CCNNNNNNNGG 5 cut(s) 49, 520, 819, 1154, 1429
BsmAI GTCTC 3 cut(s) 370, 375, 941
BsmFI GGGAC 4 cut(s) 67, 835, 1369, 1611
BsmI GAATGC 1 cut(s) 926
BsnI GGCC 7 cut(s) 53, 482, 706, 845, 956, 1146, 1298
BsoBI CYCGRG 1 cut(s) 1271
Bsp119I TTCGAA 2 cut(s) 470, 572
Bsp1286I GDGCHC 4 cut(s) 321, 446, 862, 1095
Bsp13I TCCGGA 1 cut(s) 625
Bsp143I GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
BspANI GGCC 7 cut(s) 53, 482, 706, 845, 956, 1146, 1298
BspCNI CTCAG 1 cut(s) 726
BspEI TCCGGA 1 cut(s) 625
BspFNI CGCG 5 cut(s) 456, 798, 951, 1150, 1152
BspLI GGNNCC 6 cut(s) 176, 355, 445, 823, 955, 1242
BspMAI CTGCAG 1 cut(s) 396
BspPI GGATC 4 cut(s) 260, 717, 1217, 1530
BspQI GCTCTTC 1 cut(s) 644
BspT104I TTCGAA 2 cut(s) 470, 572
BspT107I GGYRCC 2 cut(s) 353, 443
BsrDI GCAATG 1 cut(s) 807
BsrI ACTGG 4 cut(s) 418, 482, 504, 1590
BssECI CCNNGG 3 cut(s) 817, 1057, 1162
BssMI GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
BssNAI GTATAC 1 cut(s) 1551
BssNI GRCGYC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 1057
Bst1107I GTATAC 1 cut(s) 1551
Bst2BI CACGAG 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 467, 1042
Bst6I CTCTTC 3 cut(s) 121, 644, 984
BstACI GRCGYC 1 cut(s) 354
BstBAI YACGTR 2 cut(s) 751, 831
BstBI TTCGAA 2 cut(s) 470, 572
BstC8I GCNNGC 4 cut(s) 64, 392, 1148, 1417
BstDEI CTNAG 3 cut(s) 713, 1069, 1504
BstDSI CCRYGG 1 cut(s) 817
BstEII GGTNACC 1 cut(s) 1157
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 6 cut(s) 110, 508, 726, 792, 1198, 1459
BstFNI CGCG 5 cut(s) 456, 798, 951, 1150, 1152
BstH2I RGCGCY 6 cut(s) 63, 357, 391, 400, 623, 1271
BstHHI GCGC 9 cut(s) 62, 356, 390, 399, 456, 622, 1106, 1152, 1270
BstKTI GATC 9 cut(s) 250, 268, 635, 712, 1212, 1233, 1320, 1525, 1532
BstMAI GTCTC 3 cut(s) 370, 375, 941
BstMBI GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
BstMCI CGRYCG 2 cut(s) 734, 885
BstMWI GCNNNNNNNGC 7 cut(s) 59, 394, 396, 587, 1009, 1070, 1412
BstNSI RCATGY 4 cut(s) 370, 1104, 1333, 1352
BstPI GGTNACC 1 cut(s) 1157
BstSCI CCNGG 3 cut(s) 145, 446, 1243
BstSFI CTRYAG 1 cut(s) 392
BstSLI GKGCMC 1 cut(s) 446
BstUI CGCG 5 cut(s) 456, 798, 951, 1150, 1152
BstV1I GCAGC 3 cut(s) 406, 1051, 1406
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 3 cut(s) 265, 709, 1529
BstYI RGATCY 3 cut(s) 265, 709, 1529
BstZ17I GTATAC 1 cut(s) 1551
BsuRI GGCC 7 cut(s) 53, 482, 706, 845, 956, 1146, 1298
BtgI CCRYGG 1 cut(s) 817
BtgZI GCGATG 1 cut(s) 1101
BtrI CACGTC 4 cut(s) 81, 666, 961, 1228
BtsCI GGATG 6 cut(s) 110, 508, 726, 792, 1198, 1459
BtsIMutI CAGTG 2 cut(s) 497, 987
Cac8I GCNNGC 4 cut(s) 64, 392, 1148, 1417
CfoI GCGC 9 cut(s) 62, 356, 390, 399, 456, 622, 1106, 1152, 1270
Cfr13I GGNCC 6 cut(s) 14, 480, 822, 843, 954, 1145
CseI GACGC 2 cut(s) 349, 804
Csp6I GTAC 2 cut(s) 421, 748
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 7 cut(s) 367, 583, 1101, 1315, 1330, 1349, 1424
CviQI GTAC 2 cut(s) 421, 748
DdeI CTNAG 3 cut(s) 713, 1069, 1504
DinI GGCGCC 1 cut(s) 355
DpnI GATC 9 cut(s) 249, 267, 634, 711, 1211, 1232, 1319, 1524, 1531
DpnII GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
DraI TTTAAA 1 cut(s) 1581
DraIII CACNNNGTG 1 cut(s) 450
DrdI GACNNNNNNGTC 1 cut(s) 730
DseDI GACNNNNNNGTC 1 cut(s) 730
Eam1104I CTCTTC 3 cut(s) 121, 644, 984
EarI CTCTTC 3 cut(s) 121, 644, 984
EciI GGCGGA 2 cut(s) 207, 605
Ecl136II GAGCTC 2 cut(s) 860, 1093
Eco130I CCWWGG 1 cut(s) 1057
Eco24I GRGCYC 3 cut(s) 321, 862, 1095
Eco47I GGWCC 2 cut(s) 14, 822
Eco47III AGCGCT 1 cut(s) 1269
Eco53kI GAGCTC 2 cut(s) 860, 1093
Eco57I CTGAAG 2 cut(s) 52, 1502
Eco72I CACGTG 1 cut(s) 831
Eco88I CYCGRG 1 cut(s) 1271
Eco91I GGTNACC 1 cut(s) 1157
EcoICRI GAGCTC 2 cut(s) 860, 1093
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 822
EcoO65I GGTNACC 1 cut(s) 1157
EcoT14I CCWWGG 1 cut(s) 1057
EcoT38I GRGCYC 3 cut(s) 321, 862, 1095
EgeI GGCGCC 1 cut(s) 355
EheI GGCGCC 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 1057
FaeI CATG 7 cut(s) 370, 586, 1104, 1318, 1333, 1352, 1427
FaqI GGGAC 4 cut(s) 67, 835, 1369, 1611
FatI CATG 7 cut(s) 366, 582, 1100, 1314, 1329, 1348, 1423
FauI CCCGC 3 cut(s) 139, 944, 1155
FbaI TGATCA 1 cut(s) 1230
FblI GTMKAC 3 cut(s) 881, 1371, 1550
Fnu4HI GCNGC 5 cut(s) 357, 395, 552, 1065, 1420
FokI GGATG 6 cut(s) 97, 515, 733, 799, 1205, 1466
FriOI GRGCYC 3 cut(s) 321, 862, 1095
Fsp4HI GCNGC 5 cut(s) 357, 395, 552, 1065, 1420
FspBI CTAG 3 cut(s) 1236, 1575, 1620
GlaI GCGC 9 cut(s) 61, 355, 389, 398, 455, 621, 1105, 1151, 1269
GluI GCNGC 5 cut(s) 357, 395, 552, 1065, 1420
GsaI CCCAGC 3 cut(s) 29, 730, 742
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 6 cut(s) 63, 357, 391, 400, 623, 1271
HaeIII GGCC 7 cut(s) 53, 482, 706, 845, 956, 1146, 1298
HapII CCGG 8 cut(s) 147, 448, 483, 514, 626, 707, 1245, 1299
HgaI GACGC 2 cut(s) 349, 804
HhaI GCGC 9 cut(s) 62, 356, 390, 399, 456, 622, 1106, 1152, 1270
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 7 cut(s) 370, 586, 1104, 1318, 1333, 1352, 1427
Hin6I GCGC 9 cut(s) 60, 354, 388, 397, 454, 620, 1104, 1150, 1268
HinP1I GCGC 9 cut(s) 60, 354, 388, 397, 454, 620, 1104, 1150, 1268
HincII GTYRAC 5 cut(s) 40, 882, 896, 1216, 1372
HindII GTYRAC 5 cut(s) 40, 882, 896, 1216, 1372
HinfI GANTC 8 cut(s) 169, 508, 574, 934, 977, 1167, 1305, 1368
HpaII CCGG 8 cut(s) 147, 448, 483, 514, 626, 707, 1245, 1299
HphI GGTGA 2 cut(s) 120, 1377
Hpy166II GTNNAC 7 cut(s) 40, 882, 896, 1216, 1372, 1551, 1616
Hpy188I TCNGA 6 cut(s) 14, 71, 375, 530, 603, 873
Hpy188III TCNNGA 7 cut(s) 251, 285, 626, 836, 997, 1223, 1520
Hpy8I GTNNAC 7 cut(s) 40, 882, 896, 1216, 1372, 1551, 1616
Hpy99I CGWCG 4 cut(s) 14, 883, 962, 1123
HpyAV CCTTC 3 cut(s) 294, 1192, 1256
HpyCH4III ACNGT 2 cut(s) 467, 1042
HpyCH4IV ACGT 8 cut(s) 36, 80, 615, 665, 750, 830, 960, 1227
HpyCH4V TGCA 4 cut(s) 394, 782, 1406, 1444
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 394, 396, 587, 1009, 1070, 1412
HpyF3I CTNAG 3 cut(s) 713, 1069, 1504
HpySE526I ACGT 8 cut(s) 36, 80, 615, 665, 750, 830, 960, 1227
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 7 cut(s) 370, 586, 1104, 1318, 1333, 1352, 1427
HspAI GCGC 9 cut(s) 60, 354, 388, 397, 454, 620, 1104, 1150, 1268
KasI GGCGCC 1 cut(s) 353
Kpn2I TCCGGA 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 1230
Kzo9I GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
LguI GCTCTTC 1 cut(s) 644
LmnI GCTCC 6 cut(s) 27, 112, 180, 204, 1265, 1500
Lsp1109I GCAGC 3 cut(s) 406, 1051, 1406
LweI GCATC 4 cut(s) 594, 864, 1393, 1475
MaeI CTAG 3 cut(s) 1236, 1575, 1620
MaeII ACGT 8 cut(s) 36, 80, 615, 665, 750, 830, 960, 1227
MaeIII GTNAC 5 cut(s) 282, 643, 1042, 1157, 1383
MalI GATC 9 cut(s) 249, 267, 634, 711, 1211, 1232, 1319, 1524, 1531
MboI GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
MboII GAAGA 6 cut(s) 108, 111, 374, 661, 1001, 1297
MflI RGATCY 3 cut(s) 265, 709, 1529
MhlI GDGCHC 4 cut(s) 321, 446, 862, 1095
MluCI AATT 7 cut(s) 436, 850, 867, 1361, 1471, 1544, 1567
Mly113I GGCGCC 1 cut(s) 354
MlyI GAGTC 4 cut(s) 163, 943, 986, 1377
MmeI TCCRAC 1 cut(s) 698
MroI TCCGGA 1 cut(s) 625
MseI TTAA 5 cut(s) 89, 141, 854, 1031, 1580
MslI CAYNNNNRTG 5 cut(s) 164, 276, 1040, 1313, 1379
MspA1I CMGCKG 1 cut(s) 730
MspI CCGG 8 cut(s) 147, 448, 483, 514, 626, 707, 1245, 1299
MspR9I CCNGG 3 cut(s) 147, 448, 1245
Mva1269I GAATGC 1 cut(s) 926
MvnI CGCG 5 cut(s) 456, 798, 951, 1150, 1152
MwoI GCNNNNNNNGC 7 cut(s) 59, 394, 396, 587, 1009, 1070, 1412
NarI GGCGCC 1 cut(s) 354
NciI CCSGG 3 cut(s) 147, 448, 1245
NdeII GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
NlaIII CATG 7 cut(s) 370, 586, 1104, 1318, 1333, 1352, 1427
NlaIV GGNNCC 6 cut(s) 176, 355, 445, 823, 955, 1242
NmeAIII GCCGAG 5 cut(s) 151, 871, 990, 1000, 1132
NmuCI GTSAC 3 cut(s) 282, 1042, 1383
NspI RCATGY 4 cut(s) 370, 1104, 1333, 1352
NspV TTCGAA 2 cut(s) 470, 572
PaeR7I CTCGAG 1 cut(s) 1271
PciI ACATGT 2 cut(s) 366, 1348
PciSI GCTCTTC 1 cut(s) 644
PcsI WCGNNNNNNNCGW 4 cut(s) 189, 569, 681, 1317
PctI GAATGC 1 cut(s) 926
PfeI GAWTC 4 cut(s) 508, 574, 1167, 1305
PflMI CCANNNNNTGG 1 cut(s) 1429
PkrI GCNGC 5 cut(s) 358, 396, 553, 1066, 1421
PleI GAGTC 4 cut(s) 163, 942, 985, 1376
PluTI GGCGCC 1 cut(s) 357
PmaCI CACGTG 1 cut(s) 831
PmlI CACGTG 1 cut(s) 831
PpsI GAGTC 4 cut(s) 163, 942, 985, 1376
Ppu21I YACGTR 2 cut(s) 751, 831
PpuMI RGGWCCY 1 cut(s) 822
PscI ACATGT 2 cut(s) 366, 1348
Psp124BI GAGCTC 2 cut(s) 862, 1095
Psp5II RGGWCCY 1 cut(s) 822
PspCI CACGTG 1 cut(s) 831
PspEI GGTNACC 1 cut(s) 1157
PspFI CCCAGC 3 cut(s) 25, 726, 738
PspN4I GGNNCC 6 cut(s) 176, 355, 445, 823, 955, 1242
PspPI GGNCC 6 cut(s) 14, 480, 822, 843, 954, 1145
PspPPI RGGWCCY 1 cut(s) 822
PspXI VCTCGAGB 1 cut(s) 1271
PstI CTGCAG 1 cut(s) 396
PsuI RGATCY 3 cut(s) 265, 709, 1529
RsaI GTAC 2 cut(s) 422, 749
RsaNI GTAC 2 cut(s) 421, 748
RseI CAYNNNNRTG 5 cut(s) 164, 276, 1040, 1313, 1379
SacI GAGCTC 2 cut(s) 862, 1095
SalI GTCGAC 2 cut(s) 880, 1370
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 5 cut(s) 89, 141, 854, 1031, 1580
SatI GCNGC 5 cut(s) 357, 395, 552, 1065, 1420
Sau3AI GATC 9 cut(s) 247, 265, 632, 709, 1209, 1230, 1317, 1522, 1529
Sau96I GGNCC 6 cut(s) 14, 480, 822, 843, 954, 1145
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 4 cut(s) 163, 943, 986, 1377
ScrFI CCNGG 3 cut(s) 147, 448, 1245
SduI GDGCHC 4 cut(s) 321, 446, 862, 1095
SfaNI GCATC 4 cut(s) 594, 864, 1393, 1475
SfcI CTRYAG 1 cut(s) 392
SfoI GGCGCC 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 1271
SfuI TTCGAA 2 cut(s) 470, 572
SinI GGWCC 2 cut(s) 14, 822
SlaI CTCGAG 1 cut(s) 1271
SmiMI CAYNNNNRTG 5 cut(s) 164, 276, 1040, 1313, 1379
SmlI CTYRAG 3 cut(s) 383, 1221, 1271
SmoI CTYRAG 3 cut(s) 383, 1221, 1271
SpeI ACTAGT 1 cut(s) 1574
Sse9I AATT 7 cut(s) 436, 850, 867, 1361, 1471, 1544, 1567
SspDI GGCGCC 1 cut(s) 353
SspMI CTAG 3 cut(s) 1236, 1575, 1620
SstI GAGCTC 2 cut(s) 862, 1095
StyD4I CCNGG 3 cut(s) 145, 446, 1243
StyI CCWWGG 1 cut(s) 1057
TaaI ACNGT 2 cut(s) 467, 1042
TaiI ACGT 8 cut(s) 39, 83, 618, 668, 753, 833, 963, 1230
TaqII GACCGA 1 cut(s) 899
TasI AATT 7 cut(s) 436, 850, 867, 1361, 1471, 1544, 1567
TatI WGTACW 1 cut(s) 420
TauI GCSGC 2 cut(s) 359, 554
TfiI GAWTC 4 cut(s) 508, 574, 1167, 1305
Tru1I TTAA 5 cut(s) 89, 141, 854, 1031, 1580
Tru9I TTAA 5 cut(s) 89, 141, 854, 1031, 1580
TscAI CASTG 2 cut(s) 504, 987
TseFI GTSAC 3 cut(s) 282, 1042, 1383
TseI GCWGC 3 cut(s) 394, 1064, 1419
Tsp45I GTSAC 3 cut(s) 282, 1042, 1383
TspDTI ATGAA 3 cut(s) 557, 711, 1297
TspGWI ACGGA 2 cut(s) 799, 806
TspRI CASTG 2 cut(s) 504, 987
Van91I CCANNNNNTGG 1 cut(s) 1429
VpaK11BI GGWCC 2 cut(s) 14, 822
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 1 cut(s) 850
XceI RCATGY 4 cut(s) 370, 1104, 1333, 1352
XcmI CCANNNNNNNNNTGG 1 cut(s) 436
XhoI CTCGAG 1 cut(s) 1271
XmiI GTMKAC 3 cut(s) 881, 1371, 1550
XspI CTAG 3 cut(s) 1236, 1575, 1620
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.