RchiOBHm_Chr7g0204811

Phospholipase A1-Ibeta2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014430
Physical Location & Seq
Forward (+)
22494074 .. 22495903
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18333

Sequence Viewer

Length: 1623 bp
ATGCTAGTTGGGTCTCAGAGCGTGAACCTGTTTCAGGCGACAAACCGGAGCTTCAAGAACTACACGTCACCGTTGAACCCCTTAACAAGAACTCCGTCTTCTTGTTCCTATTCAGCTTCTGCCTCGAATTGTCTTACTATTAAGTCATCGGCTGCAACCTCAATCTCAAAAACTGAGTTGTCTCTCGCCAATCTCGACAAGCTGCTCCCAAAGTCGGCTCCACCCCAACCTCCCCAGCTAGACCCGCAACCCGGGATTGAAGATCGGAAAACAAAAAGGCCTACGGAAAACAAGGGAAAGGGATTTCTAGAAGGGCTCAGTCTCGCCAGGCTTTGGCCGCAGGTGAAGGCAGCGGCGGAGGAAATGTCTCCGAGGCATTTAAAGACCCTCCGGAGGCTTCTGTCCAAGACAGCAGAGTATTCTCCCCGGAATAGTTTGAGCTCCAAGTGGAGGGAGTATCACGGTAGCAAAAACTGGGCCGGACTACTTGACCCTCTTGATGAGAATCTCCGGCGAGAGGTCGTCAGGTACGGTGAGTTCATCCAGGCGGCTTACCAATCATTTCACTCGAATCCGGCTATGTCGGTGGAGGAAACTCAGATGCCACGTAACGTGGCCTTGCCTGATAGGTCATATAAGGTAACCAAAAGTCTTTATGCGACGTCAGCAGTTGGGTTGCCTGACTGGGTCGATGATGTGGCCCCAGATCTCGGGTGGATGACCCAGCGGTCGAGCTGGATCGGGTATGTGGCGGTCTGCGACGACAGGCGGGAGATTGCTCGGATGGGACGACGAGACATAGTCATCGCCCTCCGCGGAACCTCCACGTGTCTCGAGTGGGCCGAAAACATGAGGGCCCAACTCATTCAAATGCCCCCCACCGAAGAGGGTGGAGAGGAAGCACAGGCAAAGGTCGAGTGCGGGTTCCAAAGCCTCTACAAGACACCCGGCGCCCACGTGCCGAGTCTATCAGAGTCCGTGGTCGAAGAAATCAAAAGATTGAAGGAGTTGTACAAAGGTGAGCAACTGAGCATAACCGTCACGGGCCACAGCCTTGGCGCAGCCATCGCCCTCTTAGTTGGTGACGAGCTTAAAACTTGCGCTGAGGATATGCCGCCCGTGGCGGTGTTCTCTTTTGGAGGACCCCGAGTAGGAAACAGAGGCTTCGCAAACCGAATGGAAGCAAAGAACGTGAAGGTCTTGCGGATCGTGAACTCCCAAGACTTGATCACAAGGGTTCCGGGCATCTTCATTGGGGAAGGAGCGCTAGAAGAGAAGCTCAAGAACTCGAAATTCGGAGACATGCTTGACAAGAATCTGCCCTTGGCCTACGCCCACGTGGGAACAGAGTTGAGGGTCGACACAAAAATGTCACCCTACTTGAAGCCCAACGCTGATGTGGCTTGCTGCCATGACTTGGAGGCATACTTGCATTTGGTGGATGGGTTCTTGGCGTCTAACTGTCCTTTTAGAAAGAATGCTAAGAGGAGTTTAGTAAGGTTGCTTCAAGATCAAGGATCTAATGTGAAAAAATTGTATATCAGTAAGGCAAACAACTTGAGTAGTTTAAATCTTGAAAGAGAGGGATTTATGGCTAGTTGTTTGCCTAGTCCCTCTGCTTAA

Protein Analysis

540

Amino Acids

59.64

Weight (kDa)

8.96

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 268 - 416 1.7e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16820
fragaria_vesca FvH4_5g19980
malus_domestica MD04G1049800.v1.1 MD06G1050100.v1.1
prunus_persica Prupe.5G060400_v2.0.a1
pyrus_communis pycom04g04380 pycom06g04250
rosa_chinensis RchiOBHm_Chr7g0204811
rosa_laevigata RLG00000003431
rosa_multiflora Rmu_sc0003221.1_g000039
rosa_roxburghii Rroxscaffold_3G00252770
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216100 Rh7BG213200 Rh7CG229400 Rh7DG223800
rosa_wichuraiana Rw7G018740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
AasI GACNNNNNNGTC 1 cut(s) 727
AatII GACGTC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 331
AccB1I GGYRCC 1 cut(s) 950
AccB7I CCANNNNNTGG 2 cut(s) 333, 1417
AccI GTMKAC 1 cut(s) 1359
AccII CGCG 1 cut(s) 816
AccIII TCCGGA 1 cut(s) 390
AclWI GGATC 3 cut(s) 746, 1214, 1525
AcoI YGGCCR 1 cut(s) 335
AcsI RAATTY 1 cut(s) 1292
AcvI CACGTG 3 cut(s) 828, 958, 1339
AcyI GRCGYC 3 cut(s) 662, 951, 1454
AfaI GTAC 2 cut(s) 530, 1013
AfeI AGCGCT 1 cut(s) 1266
AflIII ACRYGT 2 cut(s) 63, 827
AgsI TTSAA 8 cut(s) 55, 76, 260, 869, 1003, 1384, 1508, 1577
AjiI CACGTC 1 cut(s) 66
AjnI CCWGG 2 cut(s) 326, 543
AjuI GAANNNNNNNTTGG 2 cut(s) 1307, 1339
AluBI AGCT 8 cut(s) 51, 116, 202, 238, 441, 735, 1090, 1279
AluI AGCT 8 cut(s) 51, 116, 202, 238, 441, 735, 1090, 1279
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 7 cut(s) 18, 186, 326, 372, 789, 836, 1293
AlwI GGATC 3 cut(s) 746, 1214, 1525
AlwNI CAGNNNCTG 1 cut(s) 119
Ama87I CYCGRG 4 cut(s) 251, 710, 833, 1146
Aor13HI TCCGGA 1 cut(s) 390
Aor51HI AGCGCT 1 cut(s) 1266
AoxI GGCC 9 cut(s) 278, 335, 477, 615, 699, 840, 855, 1045, 1326
ApaI GGGCCC 1 cut(s) 859
ApeKI GCWGC 5 cut(s) 152, 202, 350, 1061, 1407
ApoI RAATTY 1 cut(s) 1292
AspLEI GCGC 4 cut(s) 953, 1061, 1103, 1267
AspS9I GGNCC 7 cut(s) 477, 700, 840, 855, 856, 1045, 1142
AsuC2I CCSGG 5 cut(s) 252, 253, 427, 948, 1242
AsuHPI GGTGA 6 cut(s) 60, 355, 545, 1031, 1094, 1365
AvaI CYCGRG 4 cut(s) 251, 710, 833, 1146
AvaII GGWCC 1 cut(s) 1142
BaeGI GKGCMC 1 cut(s) 859
BanI GGYRCC 1 cut(s) 950
BanII GRGCYC 3 cut(s) 318, 443, 859
BarI GAAGNNNNNNTAC 4 cut(s) 995, 1027, 1488, 1520
BbrPI CACGTG 3 cut(s) 828, 958, 1339
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 443
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 5 cut(s) 139, 189, 362, 1073, 1394
BccI CCATC 3 cut(s) 778, 1073, 1436
BciT130I CCWGG 2 cut(s) 328, 545
BclI TGATCA 1 cut(s) 1227
BcnI CCSGG 5 cut(s) 252, 253, 427, 948, 1242
BcoDI GTCTC 7 cut(s) 18, 186, 326, 372, 789, 836, 1293
BfaI CTAG 6 cut(s) 5, 239, 308, 1268, 1596, 1608
BfoI RGCGCY 2 cut(s) 954, 1268
BfuAI ACCTGC 1 cut(s) 331
BglII AGATCT 1 cut(s) 706
BisI GCNGC 9 cut(s) 153, 203, 338, 351, 354, 549, 1062, 1115, 1408
BlsI GCNGC 9 cut(s) 154, 204, 339, 352, 355, 550, 1063, 1116, 1409
Bme1390I CCNGG 7 cut(s) 252, 253, 328, 427, 545, 948, 1242
Bme18I GGWCC 1 cut(s) 1142
BmeT110I CYCGRG 4 cut(s) 251, 710, 833, 1146
BmgBI CACGTC 1 cut(s) 66
BmgT120I GGNCC 7 cut(s) 477, 700, 840, 855, 856, 1045, 1142
BmiI GGNNCC 8 cut(s) 219, 702, 820, 857, 926, 952, 1144, 1239
BmrFI CCNGG 7 cut(s) 252, 253, 328, 427, 545, 948, 1242
BmrI ACTGGG 2 cut(s) 484, 694
BmsI GCATC 2 cut(s) 591, 1254
BmuI ACTGGG 2 cut(s) 484, 694
BpiI GAAGAC 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 1104
BpuEI CTTGAG 2 cut(s) 1265, 1579
BpuMI CCSGG 5 cut(s) 252, 253, 427, 948, 1242
BsaAI YACGTR 4 cut(s) 608, 828, 958, 1339
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 3 cut(s) 662, 951, 1454
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 8 cut(s) 251, 371, 425, 814, 978, 1054, 1119, 1323
BsaWI WCCGGW 2 cut(s) 45, 390
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 350, 380
Bse1I ACTGG 2 cut(s) 479, 689
Bse8I GATNNNNATC 1 cut(s) 504
BseAI TCCGGA 1 cut(s) 390
BseBI CCWGG 2 cut(s) 328, 545
BseDI CCNNGG 8 cut(s) 251, 371, 425, 814, 978, 1054, 1119, 1323
BseGI GGATG 4 cut(s) 540, 723, 789, 1447
BseJI GATNNNNATC 1 cut(s) 504
BseMII CTCAG 6 cut(s) 29, 165, 331, 611, 1019, 1095
BseNI ACTGG 2 cut(s) 479, 689
BseRI GAGGAG 1 cut(s) 1501
BseSI GKGCMC 1 cut(s) 859
BseXI GCAGC 5 cut(s) 139, 189, 362, 1073, 1394
BseYI CCCAGC 2 cut(s) 234, 723
Bsh1236I CGCG 1 cut(s) 816
Bsh1285I CGRYCG 1 cut(s) 731
BshFI GGCC 9 cut(s) 280, 337, 479, 617, 701, 842, 857, 1047, 1328
BshNI GGYRCC 1 cut(s) 950
BsiEI CGRYCG 1 cut(s) 731
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 4 cut(s) 251, 710, 833, 1146
BsiSI CCGG 9 cut(s) 46, 252, 391, 427, 480, 511, 575, 948, 1241
BslFI GGGAC 2 cut(s) 801, 1596
BsmAI GTCTC 7 cut(s) 18, 186, 326, 372, 789, 836, 1293
BsmFI GGGAC 2 cut(s) 801, 1596
BsmI GAATGC 1 cut(s) 1483
BsnI GGCC 9 cut(s) 280, 337, 479, 617, 701, 842, 857, 1047, 1328
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 4 cut(s) 251, 710, 833, 1146
Bsp120I GGGCCC 1 cut(s) 855
Bsp1286I GDGCHC 3 cut(s) 318, 443, 859
Bsp13I TCCGGA 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 1011
Bsp143I GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
BspANI GGCC 9 cut(s) 280, 337, 479, 617, 701, 842, 857, 1047, 1328
BspCNI CTCAG 6 cut(s) 28, 166, 330, 610, 1020, 1096
BspEI TCCGGA 1 cut(s) 390
BspFNI CGCG 1 cut(s) 816
BspLI GGNNCC 8 cut(s) 219, 702, 820, 857, 926, 952, 1144, 1239
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 3 cut(s) 746, 1214, 1525
BspT107I GGYRCC 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 1011
BsrI ACTGG 2 cut(s) 479, 689
BssECI CCNNGG 8 cut(s) 251, 371, 425, 814, 978, 1054, 1119, 1323
BssMI GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
BssNI GRCGYC 3 cut(s) 662, 951, 1454
BssT1I CCWWGG 2 cut(s) 1054, 1323
Bst2UI CCWGG 2 cut(s) 328, 545
Bst4CI ACNGT 5 cut(s) 72, 464, 533, 1039, 1463
Bst6I CTCTTC 2 cut(s) 879, 1266
BstACI GRCGYC 3 cut(s) 662, 951, 1454
BstAUI TGTACA 1 cut(s) 1011
BstBAI YACGTR 4 cut(s) 608, 828, 958, 1339
BstC8I GCNNGC 1 cut(s) 1405
BstDEI CTNAG 8 cut(s) 15, 174, 317, 597, 1028, 1075, 1104, 1482
BstDSI CCRYGG 3 cut(s) 814, 978, 1119
BstEII GGTNACC 1 cut(s) 640
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 540, 723, 789, 1447
BstFNI CGCG 1 cut(s) 816
BstH2I RGCGCY 2 cut(s) 954, 1268
BstHHI GCGC 4 cut(s) 953, 1061, 1103, 1267
BstKTI GATC 7 cut(s) 265, 709, 741, 1209, 1230, 1513, 1520
BstMAI GTCTC 7 cut(s) 18, 186, 326, 372, 789, 836, 1293
BstMBI GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
BstMCI CGRYCG 1 cut(s) 731
BstMWI GCNNNNNNNGC 5 cut(s) 244, 337, 665, 1067, 1400
BstNI CCWGG 2 cut(s) 328, 545
BstNSI RCATGY 1 cut(s) 1306
BstPI GGTNACC 1 cut(s) 640
BstSCI CCNGG 7 cut(s) 250, 251, 326, 425, 543, 946, 1240
BstSLI GKGCMC 1 cut(s) 859
BstUI CGCG 1 cut(s) 816
BstV1I GCAGC 5 cut(s) 139, 189, 362, 1073, 1394
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 2 cut(s) 706, 1517
BstXI CCANNNNNNTGG 1 cut(s) 1055
BstYI RGATCY 2 cut(s) 706, 1517
BsuRI GGCC 9 cut(s) 280, 337, 479, 617, 701, 842, 857, 1047, 1328
BtgI CCRYGG 3 cut(s) 814, 978, 1119
BtgZI GCGATG 2 cut(s) 790, 1051
BtrI CACGTC 1 cut(s) 66
BtsCI GGATG 4 cut(s) 540, 723, 789, 1447
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 1405
CaiI CAGNNNCTG 1 cut(s) 119
CfoI GCGC 4 cut(s) 953, 1061, 1103, 1267
Cfr13I GGNCC 7 cut(s) 477, 700, 840, 855, 856, 1045, 1142
Cfr42I CCGCGG 1 cut(s) 817
Cfr9I CCCGGG 1 cut(s) 251
CseI GACGC 1 cut(s) 1443
Csp6I GTAC 2 cut(s) 529, 1012
CviAII CATG 3 cut(s) 850, 1303, 1412
CviQI GTAC 2 cut(s) 529, 1012
DdeI CTNAG 8 cut(s) 15, 174, 317, 597, 1028, 1075, 1104, 1482
DinI GGCGCC 1 cut(s) 952
DpnI GATC 7 cut(s) 264, 708, 740, 1208, 1229, 1512, 1519
DpnII GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
DraI TTTAAA 2 cut(s) 381, 1569
DrdI GACNNNNNNGTC 1 cut(s) 727
DseDI GACNNNNNNGTC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 335
Eam1104I CTCTTC 2 cut(s) 879, 1266
EarI CTCTTC 2 cut(s) 879, 1266
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 441
Eco130I CCWWGG 2 cut(s) 1054, 1323
Eco147I AGGCCT 1 cut(s) 280
Eco24I GRGCYC 3 cut(s) 318, 443, 859
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 1142
Eco47III AGCGCT 1 cut(s) 1266
Eco53kI GAGCTC 1 cut(s) 441
Eco72I CACGTG 3 cut(s) 828, 958, 1339
Eco88I CYCGRG 4 cut(s) 251, 710, 833, 1146
Eco91I GGTNACC 1 cut(s) 640
EcoICRI GAGCTC 1 cut(s) 441
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoO109I RGGNCCY 2 cut(s) 855, 1142
EcoO65I GGTNACC 1 cut(s) 640
EcoRII CCWGG 2 cut(s) 326, 543
EcoT14I CCWWGG 2 cut(s) 1054, 1323
EcoT38I GRGCYC 3 cut(s) 318, 443, 859
EgeI GGCGCC 1 cut(s) 952
EheI GGCGCC 1 cut(s) 952
ErhI CCWWGG 2 cut(s) 1054, 1323
FaeI CATG 3 cut(s) 853, 1306, 1415
FaqI GGGAC 2 cut(s) 801, 1596
FatI CATG 3 cut(s) 849, 1302, 1411
FauI CCCGC 3 cut(s) 252, 762, 914
FbaI TGATCA 1 cut(s) 1227
FblI GTMKAC 1 cut(s) 1359
Fnu4HI GCNGC 9 cut(s) 153, 203, 338, 351, 354, 549, 1062, 1115, 1408
FokI GGATG 4 cut(s) 527, 730, 796, 1454
FriOI GRGCYC 3 cut(s) 318, 443, 859
Fsp4HI GCNGC 9 cut(s) 153, 203, 338, 351, 354, 549, 1062, 1115, 1408
FspBI CTAG 6 cut(s) 5, 239, 308, 1268, 1596, 1608
GlaI GCGC 4 cut(s) 952, 1060, 1102, 1266
GluI GCNGC 9 cut(s) 153, 203, 338, 351, 354, 549, 1062, 1115, 1408
GsaI CCCAGC 2 cut(s) 238, 727
HaeII RGCGCY 2 cut(s) 954, 1268
HaeIII GGCC 9 cut(s) 280, 337, 479, 617, 701, 842, 857, 1047, 1328
HapII CCGG 9 cut(s) 46, 252, 391, 427, 480, 511, 575, 948, 1241
HgaI GACGC 1 cut(s) 1443
HhaI GCGC 4 cut(s) 953, 1061, 1103, 1267
Hin1I GRCGYC 3 cut(s) 662, 951, 1454
Hin1II CATG 3 cut(s) 853, 1306, 1415
Hin6I GCGC 4 cut(s) 951, 1059, 1101, 1265
HinP1I GCGC 4 cut(s) 951, 1059, 1101, 1265
HincII GTYRAC 1 cut(s) 1360
HindII GTYRAC 1 cut(s) 1360
HinfI GANTC 5 cut(s) 505, 571, 964, 974, 1315
HpaII CCGG 9 cut(s) 46, 252, 391, 427, 480, 511, 575, 948, 1241
HphI GGTGA 6 cut(s) 60, 355, 545, 1031, 1094, 1365
Hpy166II GTNNAC 3 cut(s) 25, 1213, 1360
Hpy188I TCNGA 7 cut(s) 18, 267, 372, 600, 783, 973, 1298
Hpy8I GTNNAC 3 cut(s) 25, 1213, 1360
Hpy99I CGWCG 3 cut(s) 664, 764, 795
HpyAV CCTTC 5 cut(s) 305, 340, 997, 1189, 1253
HpyCH4III ACNGT 5 cut(s) 72, 464, 533, 1039, 1463
HpyCH4IV ACGT 8 cut(s) 65, 607, 612, 662, 827, 957, 1191, 1338
HpyCH4V TGCA 2 cut(s) 155, 1432
HpyF10VI GCNNNNNNNGC 5 cut(s) 244, 337, 665, 1067, 1400
HpyF3I CTNAG 8 cut(s) 15, 174, 317, 597, 1028, 1075, 1104, 1482
HpySE526I ACGT 8 cut(s) 65, 607, 612, 662, 827, 957, 1191, 1338
Hsp92I GRCGYC 3 cut(s) 662, 951, 1454
Hsp92II CATG 3 cut(s) 853, 1306, 1415
HspAI GCGC 4 cut(s) 951, 1059, 1101, 1265
KasI GGCGCC 1 cut(s) 950
Kpn2I TCCGGA 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 1227
KspI CCGCGG 1 cut(s) 817
Kzo9I GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
LmnI GCTCC 5 cut(s) 48, 210, 223, 446, 1262
Lsp1109I GCAGC 5 cut(s) 139, 189, 362, 1073, 1394
LweI GCATC 2 cut(s) 591, 1254
MaeI CTAG 6 cut(s) 5, 239, 308, 1268, 1596, 1608
MaeII ACGT 8 cut(s) 65, 607, 612, 662, 827, 957, 1191, 1338
MaeIII GTNAC 6 cut(s) 66, 608, 640, 1039, 1082, 1371
MalI GATC 7 cut(s) 264, 708, 740, 1208, 1229, 1512, 1519
MboI GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
MboII GAAGA 6 cut(s) 90, 272, 896, 998, 1240, 1283
MflI RGATCY 2 cut(s) 706, 1517
MhlI GDGCHC 3 cut(s) 318, 443, 859
MluCI AATT 3 cut(s) 127, 1292, 1532
Mly113I GGCGCC 1 cut(s) 951
MlyI GAGTC 2 cut(s) 973, 983
MroI TCCGGA 1 cut(s) 390
MseI TTAA 6 cut(s) 83, 141, 380, 1092, 1568, 1621
MslI CAYNNNNRTG 2 cut(s) 869, 1367
MspA1I CMGCKG 3 cut(s) 353, 727, 816
MspI CCGG 9 cut(s) 46, 252, 391, 427, 480, 511, 575, 948, 1241
MspR9I CCNGG 7 cut(s) 252, 253, 328, 427, 545, 948, 1242
Mva1269I GAATGC 1 cut(s) 1483
MvaI CCWGG 2 cut(s) 328, 545
MvnI CGCG 1 cut(s) 816
MwoI GCNNNNNNNGC 5 cut(s) 244, 337, 665, 1067, 1400
NarI GGCGCC 1 cut(s) 951
NciI CCSGG 5 cut(s) 252, 253, 427, 948, 1242
NdeII GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
NlaIII CATG 3 cut(s) 853, 1306, 1415
NlaIV GGNNCC 8 cut(s) 219, 702, 820, 857, 926, 952, 1144, 1239
NmeAIII GCCGAG 1 cut(s) 987
NmuCI GTSAC 4 cut(s) 66, 1039, 1082, 1371
NspI RCATGY 1 cut(s) 1306
PaeR7I CTCGAG 1 cut(s) 833
PaqCI CACCTGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 280
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 787, 840
PctI GAATGC 1 cut(s) 1483
PfeI GAWTC 3 cut(s) 505, 571, 1315
PflFI GACNNNGTC 2 cut(s) 686, 800
PflMI CCANNNNNTGG 2 cut(s) 333, 1417
PkrI GCNGC 9 cut(s) 154, 204, 339, 352, 355, 550, 1063, 1116, 1409
PleI GAGTC 2 cut(s) 972, 982
PluTI GGCGCC 1 cut(s) 954
PmaCI CACGTG 3 cut(s) 828, 958, 1339
PmlI CACGTG 3 cut(s) 828, 958, 1339
PpsI GAGTC 2 cut(s) 972, 982
Ppu21I YACGTR 4 cut(s) 608, 828, 958, 1339
PpuMI RGGWCCY 1 cut(s) 1142
Psp124BI GAGCTC 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 1142
Psp6I CCWGG 2 cut(s) 326, 543
PspCI CACGTG 3 cut(s) 828, 958, 1339
PspEI GGTNACC 1 cut(s) 640
PspFI CCCAGC 2 cut(s) 234, 723
PspGI CCWGG 2 cut(s) 326, 543
PspN4I GGNNCC 8 cut(s) 219, 702, 820, 857, 926, 952, 1144, 1239
PspOMI GGGCCC 1 cut(s) 855
PspPI GGNCC 7 cut(s) 477, 700, 840, 855, 856, 1045, 1142
PspPPI RGGWCCY 1 cut(s) 1142
PsrI GAACNNNNNNTAC 2 cut(s) 521, 553
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 2 cut(s) 706, 1517
PsyI GACNNNGTC 2 cut(s) 686, 800
RsaI GTAC 2 cut(s) 530, 1013
RsaNI GTAC 2 cut(s) 529, 1012
RseI CAYNNNNRTG 2 cut(s) 869, 1367
SacI GAGCTC 1 cut(s) 443
SacII CCGCGG 1 cut(s) 817
SalI GTCGAC 1 cut(s) 1358
SaqAI TTAA 6 cut(s) 83, 141, 380, 1092, 1568, 1621
SatI GCNGC 9 cut(s) 153, 203, 338, 351, 354, 549, 1062, 1115, 1408
Sau3AI GATC 7 cut(s) 262, 706, 738, 1206, 1227, 1510, 1517
Sau96I GGNCC 7 cut(s) 477, 700, 840, 855, 856, 1045, 1142
SchI GAGTC 2 cut(s) 973, 983
ScrFI CCNGG 7 cut(s) 252, 253, 328, 427, 545, 948, 1242
SduI GDGCHC 3 cut(s) 318, 443, 859
SfaNI GCATC 2 cut(s) 591, 1254
SfoI GGCGCC 1 cut(s) 952
Sfr274I CTCGAG 1 cut(s) 833
Sfr303I CCGCGG 1 cut(s) 817
SgrBI CCGCGG 1 cut(s) 817
SinI GGWCC 1 cut(s) 1142
SlaI CTCGAG 1 cut(s) 833
SmaI CCCGGG 1 cut(s) 253
SmiMI CAYNNNNRTG 2 cut(s) 869, 1367
SmlI CTYRAG 3 cut(s) 833, 1280, 1558
SmoI CTYRAG 3 cut(s) 833, 1280, 1558
Sse9I AATT 3 cut(s) 127, 1292, 1532
SseBI AGGCCT 1 cut(s) 280
SspDI GGCGCC 1 cut(s) 950
SspMI CTAG 6 cut(s) 5, 239, 308, 1268, 1596, 1608
SstI GAGCTC 1 cut(s) 443
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 7 cut(s) 250, 251, 326, 425, 543, 946, 1240
StyI CCWWGG 2 cut(s) 1054, 1323
TaaI ACNGT 5 cut(s) 72, 464, 533, 1039, 1463
TaiI ACGT 8 cut(s) 68, 610, 615, 665, 830, 960, 1194, 1341
TasI AATT 3 cut(s) 127, 1292, 1532
TatI WGTACW 1 cut(s) 1011
TauI GCSGC 4 cut(s) 340, 356, 551, 1117
TfiI GAWTC 3 cut(s) 505, 571, 1315
Tru1I TTAA 6 cut(s) 83, 141, 380, 1092, 1568, 1621
Tru9I TTAA 6 cut(s) 83, 141, 380, 1092, 1568, 1621
TseFI GTSAC 4 cut(s) 66, 1039, 1082, 1371
TseI GCWGC 5 cut(s) 152, 202, 350, 1061, 1407
Tsp45I GTSAC 4 cut(s) 66, 1039, 1082, 1371
TspDTI ATGAA 2 cut(s) 529, 1240
TspGWI ACGGA 3 cut(s) 84, 299, 967
TspMI CCCGGG 1 cut(s) 251
Tth111I GACNNNGTC 2 cut(s) 686, 800
Van91I CCANNNNNTGG 2 cut(s) 333, 1417
VpaK11BI GGWCC 1 cut(s) 1142
XagI CCTNNNNNAGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 1292
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 1 cut(s) 1306
XcmI CCANNNNNNNNNTGG 2 cut(s) 711, 1396
XhoI CTCGAG 1 cut(s) 833
XmaI CCCGGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 1359
XspI CTAG 6 cut(s) 5, 239, 308, 1268, 1596, 1608
ZraI GACGTC 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.