Rw7G018740

Phospholipase A1-Ibeta2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014430
Physical Location & Seq
Forward (+)
20441499 .. 20443379
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018740.1

Sequence Viewer

Length: 1632 bp
ATGCTAGTTGGGTCTCAAAGCGCGAACCTGTTTCAGGCGACAAACCGGAGCTTCAAGAACTACACGTCACCGTTGAACCCCTTAACAAGAACTCCGTACCCCTTAACAAGAACTCCGTCTTCTTGTTCCGCCTCGAATTGTCTTTCTATTAAGTCATCGGCTGCAACCTCAATCTCAAAAACTGAGTTGACTCTATCCAATCTCGACAAGCTGCTCCCAAAGTCGGCTCCACCCCAACCTCCCCAGCTAGACCCGCAACCCGGGATTGAAGATCGGAAAACAAAAAGGCCTACGGAAAACAAGGGAAAGGGATTTCTGGAAGGGCTCAGTCTCGCCAGGCTTTGGCCGCAGGTGAAGGCAGCGGCGGAGGAAATGTCTCCGAGGCATTTGAAGACCCTCCGGAGGCTTCTGTCCAAGACAGCAGAGTATTCTCCCCGGAATAGTTTGAGCTCCAAGTGGAGGGAGTATCACGGTAGCAAAAACTGGGCCGGACTACTTGACCCTCTTGATGAGAATCTCCGGCGAGAGGTCGTCAGGTACGGTGAGTTCATCCAGGCGGCTTACCAATCATTTCACTCGAATCCGGCTATGTCGGTGGAGGAAACTCAGATGCCACGTAACGTGGCCTTGCCTGATAGGTCATATAAGGTAACCAAAAGTCTTTATGCGACGTCAGCAATCGGGTTGCCTGACTGGGTCGATGATGTGGCCCCGGATCTCGGGTGGATGACCCAGCGGTCGAGCTGGATCGGGTATGTGGCGGTCTGCGACGACAGGCGGGAGATTGCTCGGATGGGACGACGAGACATAGTCATCGCCCTCCGCGGAACCTCCACGTGTCTCGAGTGGGCCGAAAACATGAGGGCCCAACTCATTCAACTGCCCCCCACCGAAGAGGGTGGAGAGGAAGCACAGGCAAAGGTGGAGTGCGGGTTCCAAAGCCTCTACAAGACGCCCGGCGCCCACGTGCCGAGTCTATCAGAGTCCGTGGTCGAAGAAATCAGAAGATTGAAGGAGTTGTACAAAGGTGAGCAACTGAGCATAACCGTCACGGGCCACAGCCTTGGCGCAGCCATCGCCCTCTTAGTTGGTGACGAGCTTAAAACTTGCGCTGAGGATATGCCGCCCGTGGCGGTGTTCTCTTTCGGAGGACCCCGAGTAGGAAACAGAGGCTTCGCAAACCGAATGGAAGCAAAGAACGTGAAGGTCTTGCGGATCGTAAACTCCCAAGACTTGATCACAAGGGTTCCGGGCATCTTCATTGGGGAAGGAGTGCTAGAAGAGAAGCTCAAGAACTCGAAATTCGGAGACATGCTTGACAAGAATCTGCCCTTGGCTTACGCCCACGTGGGAACAGAGTTGAGGGTCGACACAAAAATGTCACCCTACTTGAAGCCCAACGCTGATGTGGCTTGCTGCCATGACTTGGAGGCATACTTGCATTTGGTGGATGGGTTCTTGGCGTCTAACTGTCCTTTTAGAAAGAATGCTAAGAGGAGTTTAGTAAGGTTGCTTCAAGATCAAGGATCTAATGTGAAAAAATTGTATATCAGTAAGGCGAACAACTTGAGTAGTTTGAATCTTGAAAGAGAGGGATTTATGGCTAGTTGTTTGCCTAGTCCCTCTTCTTAA

Protein Analysis

543

Amino Acids

60.11

Weight (kDa)

9.03

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 271 - 419 5.3e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16820
fragaria_vesca FvH4_5g19980
malus_domestica MD04G1049800.v1.1 MD06G1050100.v1.1
prunus_persica Prupe.5G060400_v2.0.a1
pyrus_communis pycom04g04380 pycom06g04250
rosa_chinensis RchiOBHm_Chr7g0204811
rosa_laevigata RLG00000003431
rosa_multiflora Rmu_sc0003221.1_g000039
rosa_roxburghii Rroxscaffold_3G00252770
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216100 Rh7BG213200 Rh7CG229400 Rh7DG223800
rosa_wichuraiana Rw7G018740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
AasI GACNNNNNNGTC 1 cut(s) 736
AatII GACGTC 1 cut(s) 674
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 959
AccB7I CCANNNNNTGG 2 cut(s) 342, 1426
AccI GTMKAC 1 cut(s) 1368
AccII CGCG 2 cut(s) 23, 825
AccIII TCCGGA 1 cut(s) 399
AclWI GGATC 4 cut(s) 723, 755, 1223, 1534
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 1 cut(s) 1301
AcvI CACGTG 3 cut(s) 837, 967, 1348
AcyI GRCGYC 4 cut(s) 671, 953, 960, 1463
AfaI GTAC 3 cut(s) 98, 539, 1022
AflIII ACRYGT 2 cut(s) 63, 836
AjiI CACGTC 1 cut(s) 66
AjnI CCWGG 2 cut(s) 335, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 1316, 1348
AloI GAACNNNNNNTCC 2 cut(s) 917, 949
AluBI AGCT 7 cut(s) 51, 211, 247, 450, 744, 1099, 1288
AluI AGCT 7 cut(s) 51, 211, 247, 450, 744, 1099, 1288
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 6 cut(s) 18, 335, 381, 798, 845, 1302
AlwI GGATC 4 cut(s) 723, 755, 1223, 1534
Ama87I CYCGRG 4 cut(s) 260, 719, 842, 1155
Aor13HI TCCGGA 1 cut(s) 399
AoxI GGCC 8 cut(s) 287, 344, 486, 624, 708, 849, 864, 1054
ApaI GGGCCC 1 cut(s) 868
ApeKI GCWGC 5 cut(s) 161, 211, 359, 1070, 1416
ApoI RAATTY 1 cut(s) 1301
AspLEI GCGC 4 cut(s) 23, 962, 1070, 1112
AspS9I GGNCC 7 cut(s) 486, 709, 849, 864, 865, 1054, 1151
AsuC2I CCSGG 6 cut(s) 261, 262, 436, 713, 957, 1251
AsuHPI GGTGA 6 cut(s) 60, 364, 554, 1040, 1103, 1374
AvaI CYCGRG 4 cut(s) 260, 719, 842, 1155
AvaII GGWCC 1 cut(s) 1151
BaeGI GKGCMC 1 cut(s) 868
BanI GGYRCC 1 cut(s) 959
BanII GRGCYC 3 cut(s) 327, 452, 868
BarI GAAGNNNNNNTAC 4 cut(s) 1004, 1036, 1497, 1529
BbrPI CACGTG 3 cut(s) 837, 967, 1348
BbsI GAAGAC 2 cut(s) 111, 398
Bbv12I GWGCWC 1 cut(s) 452
BbvCI CCTCAGC 1 cut(s) 1113
BbvI GCAGC 5 cut(s) 148, 198, 371, 1082, 1403
BccI CCATC 3 cut(s) 787, 1082, 1445
BciT130I CCWGG 2 cut(s) 337, 554
BclI TGATCA 1 cut(s) 1236
BcnI CCSGG 6 cut(s) 261, 262, 436, 713, 957, 1251
BcoDI GTCTC 6 cut(s) 18, 335, 381, 798, 845, 1302
BfaI CTAG 5 cut(s) 5, 248, 1277, 1605, 1617
BfoI RGCGCY 1 cut(s) 963
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 9 cut(s) 162, 212, 347, 360, 363, 558, 1071, 1124, 1417
BlsI GCNGC 9 cut(s) 163, 213, 348, 361, 364, 559, 1072, 1125, 1418
Bme1390I CCNGG 8 cut(s) 261, 262, 337, 436, 554, 713, 957, 1251
Bme18I GGWCC 1 cut(s) 1151
BmeT110I CYCGRG 4 cut(s) 260, 719, 842, 1155
BmgBI CACGTC 1 cut(s) 66
BmgT120I GGNCC 7 cut(s) 486, 709, 849, 864, 865, 1054, 1151
BmiI GGNNCC 8 cut(s) 228, 711, 829, 866, 935, 961, 1153, 1248
BmrFI CCNGG 8 cut(s) 261, 262, 337, 436, 554, 713, 957, 1251
BmrI ACTGGG 2 cut(s) 493, 703
BmsI GCATC 2 cut(s) 600, 1263
BmuI ACTGGG 2 cut(s) 493, 703
BpiI GAAGAC 2 cut(s) 111, 398
Bpu10I CCTNAGC 1 cut(s) 1113
BpuEI CTTGAG 2 cut(s) 1274, 1588
BpuMI CCSGG 6 cut(s) 261, 262, 436, 713, 957, 1251
BsaAI YACGTR 4 cut(s) 617, 837, 967, 1348
BsaBI GATNNNNATC 1 cut(s) 513
BsaHI GRCGYC 4 cut(s) 671, 953, 960, 1463
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 9 cut(s) 260, 380, 434, 711, 823, 987, 1063, 1128, 1332
BsaWI WCCGGW 2 cut(s) 45, 399
BsaXI ACNNNNNCTCC 8 cut(s) 76, 97, 106, 127, 359, 389, 917, 947
Bse1I ACTGG 2 cut(s) 488, 698
Bse8I GATNNNNATC 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 399
BseBI CCWGG 2 cut(s) 337, 554
BseDI CCNNGG 9 cut(s) 260, 380, 434, 711, 823, 987, 1063, 1128, 1332
BseGI GGATG 4 cut(s) 549, 732, 798, 1456
BseJI GATNNNNATC 1 cut(s) 513
BseMII CTCAG 5 cut(s) 174, 340, 620, 1028, 1104
BseNI ACTGG 2 cut(s) 488, 698
BseRI GAGGAG 1 cut(s) 1510
BseSI GKGCMC 1 cut(s) 868
BseXI GCAGC 5 cut(s) 148, 198, 371, 1082, 1403
BseYI CCCAGC 2 cut(s) 243, 732
Bsh1236I CGCG 2 cut(s) 23, 825
Bsh1285I CGRYCG 1 cut(s) 740
BshFI GGCC 8 cut(s) 289, 346, 488, 626, 710, 851, 866, 1056
BshNI GGYRCC 1 cut(s) 959
BsiEI CGRYCG 1 cut(s) 740
BsiHKAI GWGCWC 1 cut(s) 452
BsiHKCI CYCGRG 4 cut(s) 260, 719, 842, 1155
BslFI GGGAC 2 cut(s) 810, 1605
BsmAI GTCTC 6 cut(s) 18, 335, 381, 798, 845, 1302
BsmFI GGGAC 2 cut(s) 810, 1605
BsmI GAATGC 1 cut(s) 1492
BsnI GGCC 8 cut(s) 289, 346, 488, 626, 710, 851, 866, 1056
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 4 cut(s) 260, 719, 842, 1155
Bsp120I GGGCCC 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 327, 452, 868
Bsp13I TCCGGA 1 cut(s) 399
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
BspANI GGCC 8 cut(s) 289, 346, 488, 626, 710, 851, 866, 1056
BspCNI CTCAG 5 cut(s) 175, 339, 619, 1029, 1105
BspEI TCCGGA 1 cut(s) 399
BspFNI CGCG 2 cut(s) 23, 825
BspLI GGNNCC 8 cut(s) 228, 711, 829, 866, 935, 961, 1153, 1248
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 4 cut(s) 723, 755, 1223, 1534
BspT107I GGYRCC 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 2 cut(s) 488, 698
BssECI CCNNGG 9 cut(s) 260, 380, 434, 711, 823, 987, 1063, 1128, 1332
BssMI GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
BssNI GRCGYC 4 cut(s) 671, 953, 960, 1463
BssT1I CCWWGG 2 cut(s) 1063, 1332
Bst2UI CCWGG 2 cut(s) 337, 554
Bst4CI ACNGT 5 cut(s) 72, 473, 542, 1048, 1472
Bst6I CTCTTC 2 cut(s) 888, 1275
BstACI GRCGYC 4 cut(s) 671, 953, 960, 1463
BstAUI TGTACA 1 cut(s) 1020
BstBAI YACGTR 4 cut(s) 617, 837, 967, 1348
BstC8I GCNNGC 1 cut(s) 1414
BstDEI CTNAG 7 cut(s) 183, 326, 606, 1037, 1084, 1113, 1491
BstDSI CCRYGG 3 cut(s) 823, 987, 1128
BstEII GGTNACC 1 cut(s) 649
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 549, 732, 798, 1456
BstFNI CGCG 2 cut(s) 23, 825
BstH2I RGCGCY 1 cut(s) 963
BstHHI GCGC 4 cut(s) 23, 962, 1070, 1112
BstKTI GATC 7 cut(s) 274, 718, 750, 1218, 1239, 1522, 1529
BstMAI GTCTC 6 cut(s) 18, 335, 381, 798, 845, 1302
BstMBI GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
BstMCI CGRYCG 1 cut(s) 740
BstMWI GCNNNNNNNGC 5 cut(s) 253, 346, 674, 1076, 1409
BstNI CCWGG 2 cut(s) 337, 554
BstNSI RCATGY 1 cut(s) 1315
BstPI GGTNACC 1 cut(s) 649
BstSCI CCNGG 8 cut(s) 259, 260, 335, 434, 552, 711, 955, 1249
BstSLI GKGCMC 1 cut(s) 868
BstUI CGCG 2 cut(s) 23, 825
BstV1I GCAGC 5 cut(s) 148, 198, 371, 1082, 1403
BstV2I GAAGAC 2 cut(s) 111, 398
BstX2I RGATCY 2 cut(s) 715, 1526
BstXI CCANNNNNNTGG 1 cut(s) 1064
BstYI RGATCY 2 cut(s) 715, 1526
BsuRI GGCC 8 cut(s) 289, 346, 488, 626, 710, 851, 866, 1056
BtgI CCRYGG 3 cut(s) 823, 987, 1128
BtgZI GCGATG 2 cut(s) 799, 1060
BtrI CACGTC 1 cut(s) 66
BtsCI GGATG 4 cut(s) 549, 732, 798, 1456
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 1414
CfoI GCGC 4 cut(s) 23, 962, 1070, 1112
Cfr13I GGNCC 7 cut(s) 486, 709, 849, 864, 865, 1054, 1151
Cfr42I CCGCGG 1 cut(s) 826
Cfr9I CCCGGG 1 cut(s) 260
CseI GACGC 2 cut(s) 961, 1452
Csp6I GTAC 3 cut(s) 97, 538, 1021
CviAII CATG 3 cut(s) 859, 1312, 1421
CviQI GTAC 3 cut(s) 97, 538, 1021
DdeI CTNAG 7 cut(s) 183, 326, 606, 1037, 1084, 1113, 1491
DinI GGCGCC 1 cut(s) 961
DpnI GATC 7 cut(s) 273, 717, 749, 1217, 1238, 1521, 1528
DpnII GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
DrdI GACNNNNNNGTC 1 cut(s) 736
DseDI GACNNNNNNGTC 1 cut(s) 736
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 888, 1275
EarI CTCTTC 2 cut(s) 888, 1275
EciI GGCGGA 2 cut(s) 118, 380
Ecl136II GAGCTC 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 1063, 1332
Eco147I AGGCCT 1 cut(s) 289
Eco24I GRGCYC 3 cut(s) 327, 452, 868
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 1151
Eco53kI GAGCTC 1 cut(s) 450
Eco72I CACGTG 3 cut(s) 837, 967, 1348
Eco88I CYCGRG 4 cut(s) 260, 719, 842, 1155
Eco91I GGTNACC 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 450
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoO109I RGGNCCY 2 cut(s) 864, 1151
EcoO65I GGTNACC 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 335, 552
EcoT14I CCWWGG 2 cut(s) 1063, 1332
EcoT38I GRGCYC 3 cut(s) 327, 452, 868
EgeI GGCGCC 1 cut(s) 961
EheI GGCGCC 1 cut(s) 961
ErhI CCWWGG 2 cut(s) 1063, 1332
FaeI CATG 3 cut(s) 862, 1315, 1424
FaqI GGGAC 2 cut(s) 810, 1605
FatI CATG 3 cut(s) 858, 1311, 1420
FauI CCCGC 3 cut(s) 261, 771, 923
FbaI TGATCA 1 cut(s) 1236
FblI GTMKAC 1 cut(s) 1368
Fnu4HI GCNGC 9 cut(s) 162, 212, 347, 360, 363, 558, 1071, 1124, 1417
FokI GGATG 4 cut(s) 536, 739, 805, 1463
FriOI GRGCYC 3 cut(s) 327, 452, 868
Fsp4HI GCNGC 9 cut(s) 162, 212, 347, 360, 363, 558, 1071, 1124, 1417
FspBI CTAG 5 cut(s) 5, 248, 1277, 1605, 1617
GlaI GCGC 4 cut(s) 22, 961, 1069, 1111
GluI GCNGC 9 cut(s) 162, 212, 347, 360, 363, 558, 1071, 1124, 1417
GsaI CCCAGC 2 cut(s) 247, 736
HaeII RGCGCY 1 cut(s) 963
HaeIII GGCC 8 cut(s) 289, 346, 488, 626, 710, 851, 866, 1056
HgaI GACGC 2 cut(s) 961, 1452
HhaI GCGC 4 cut(s) 23, 962, 1070, 1112
Hin1I GRCGYC 4 cut(s) 671, 953, 960, 1463
Hin1II CATG 3 cut(s) 862, 1315, 1424
Hin6I GCGC 4 cut(s) 21, 960, 1068, 1110
HinP1I GCGC 4 cut(s) 21, 960, 1068, 1110
HincII GTYRAC 2 cut(s) 189, 1369
HindII GTYRAC 2 cut(s) 189, 1369
HinfI GANTC 7 cut(s) 190, 514, 580, 973, 983, 1324, 1579
HphI GGTGA 6 cut(s) 60, 364, 554, 1040, 1103, 1374
Hpy166II GTNNAC 3 cut(s) 189, 1222, 1369
Hpy188I TCNGA 8 cut(s) 276, 381, 609, 792, 982, 1004, 1148, 1307
Hpy188III TCNNGA 9 cut(s) 55, 203, 317, 400, 506, 842, 1291, 1517, 1583
Hpy8I GTNNAC 3 cut(s) 189, 1222, 1369
Hpy99I CGWCG 3 cut(s) 673, 773, 804
HpyAV CCTTC 5 cut(s) 314, 349, 1006, 1198, 1262
HpyCH4III ACNGT 5 cut(s) 72, 473, 542, 1048, 1472
HpyCH4IV ACGT 8 cut(s) 65, 616, 621, 671, 836, 966, 1200, 1347
HpyCH4V TGCA 2 cut(s) 164, 1441
HpyF10VI GCNNNNNNNGC 5 cut(s) 253, 346, 674, 1076, 1409
HpyF3I CTNAG 7 cut(s) 183, 326, 606, 1037, 1084, 1113, 1491
HpySE526I ACGT 8 cut(s) 65, 616, 621, 671, 836, 966, 1200, 1347
Hsp92I GRCGYC 4 cut(s) 671, 953, 960, 1463
Hsp92II CATG 3 cut(s) 862, 1315, 1424
HspAI GCGC 4 cut(s) 21, 960, 1068, 1110
KasI GGCGCC 1 cut(s) 959
Kpn2I TCCGGA 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 1236
KspI CCGCGG 1 cut(s) 826
Kzo9I GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
LmnI GCTCC 4 cut(s) 48, 219, 232, 455
Lsp1109I GCAGC 5 cut(s) 148, 198, 371, 1082, 1403
LweI GCATC 2 cut(s) 600, 1263
MaeI CTAG 5 cut(s) 5, 248, 1277, 1605, 1617
MaeII ACGT 8 cut(s) 65, 616, 621, 671, 836, 966, 1200, 1347
MaeIII GTNAC 6 cut(s) 66, 617, 649, 1048, 1091, 1380
MalI GATC 7 cut(s) 273, 717, 749, 1217, 1238, 1521, 1528
MboI GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
MboII GAAGA 9 cut(s) 111, 281, 403, 905, 1007, 1017, 1249, 1292, 1617
MflI RGATCY 2 cut(s) 715, 1526
MhlI GDGCHC 3 cut(s) 327, 452, 868
MluCI AATT 3 cut(s) 136, 1301, 1541
Mly113I GGCGCC 1 cut(s) 960
MlyI GAGTC 3 cut(s) 184, 982, 992
MroI TCCGGA 1 cut(s) 399
MseI TTAA 5 cut(s) 83, 104, 150, 1101, 1630
MslI CAYNNNNRTG 1 cut(s) 1376
MspA1I CMGCKG 3 cut(s) 362, 736, 825
MspR9I CCNGG 8 cut(s) 261, 262, 337, 436, 554, 713, 957, 1251
Mva1269I GAATGC 1 cut(s) 1492
MvaI CCWGG 2 cut(s) 337, 554
MvnI CGCG 2 cut(s) 23, 825
MwoI GCNNNNNNNGC 5 cut(s) 253, 346, 674, 1076, 1409
NarI GGCGCC 1 cut(s) 960
NciI CCSGG 6 cut(s) 261, 262, 436, 713, 957, 1251
NdeII GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
NlaIII CATG 3 cut(s) 862, 1315, 1424
NlaIV GGNNCC 8 cut(s) 228, 711, 829, 866, 935, 961, 1153, 1248
NmeAIII GCCGAG 1 cut(s) 996
NmuCI GTSAC 4 cut(s) 66, 1048, 1091, 1380
NspI RCATGY 1 cut(s) 1315
PaeR7I CTCGAG 1 cut(s) 842
PaqCI CACCTGC 1 cut(s) 340
PceI AGGCCT 1 cut(s) 289
PcsI WCGNNNNNNNCGW 2 cut(s) 796, 849
PctI GAATGC 1 cut(s) 1492
PfeI GAWTC 4 cut(s) 514, 580, 1324, 1579
PflFI GACNNNGTC 2 cut(s) 695, 809
PflMI CCANNNNNTGG 2 cut(s) 342, 1426
PkrI GCNGC 9 cut(s) 163, 213, 348, 361, 364, 559, 1072, 1125, 1418
PleI GAGTC 3 cut(s) 184, 981, 991
PluTI GGCGCC 1 cut(s) 963
PmaCI CACGTG 3 cut(s) 837, 967, 1348
PmlI CACGTG 3 cut(s) 837, 967, 1348
PpsI GAGTC 3 cut(s) 184, 981, 991
Ppu21I YACGTR 4 cut(s) 617, 837, 967, 1348
PpuMI RGGWCCY 1 cut(s) 1151
Psp124BI GAGCTC 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 1151
Psp6I CCWGG 2 cut(s) 335, 552
PspCI CACGTG 3 cut(s) 837, 967, 1348
PspEI GGTNACC 1 cut(s) 649
PspFI CCCAGC 2 cut(s) 243, 732
PspGI CCWGG 2 cut(s) 335, 552
PspN4I GGNNCC 8 cut(s) 228, 711, 829, 866, 935, 961, 1153, 1248
PspOMI GGGCCC 1 cut(s) 864
PspPI GGNCC 7 cut(s) 486, 709, 849, 864, 865, 1054, 1151
PspPPI RGGWCCY 1 cut(s) 1151
PsrI GAACNNNNNNTAC 2 cut(s) 530, 562
PsuI RGATCY 2 cut(s) 715, 1526
PsyI GACNNNGTC 2 cut(s) 695, 809
RsaI GTAC 3 cut(s) 98, 539, 1022
RsaNI GTAC 3 cut(s) 97, 538, 1021
RseI CAYNNNNRTG 1 cut(s) 1376
SacI GAGCTC 1 cut(s) 452
SacII CCGCGG 1 cut(s) 826
SalI GTCGAC 1 cut(s) 1367
SaqAI TTAA 5 cut(s) 83, 104, 150, 1101, 1630
SatI GCNGC 9 cut(s) 162, 212, 347, 360, 363, 558, 1071, 1124, 1417
Sau3AI GATC 7 cut(s) 271, 715, 747, 1215, 1236, 1519, 1526
Sau96I GGNCC 7 cut(s) 486, 709, 849, 864, 865, 1054, 1151
SchI GAGTC 3 cut(s) 184, 982, 992
ScrFI CCNGG 8 cut(s) 261, 262, 337, 436, 554, 713, 957, 1251
SduI GDGCHC 3 cut(s) 327, 452, 868
SfaNI GCATC 2 cut(s) 600, 1263
SfoI GGCGCC 1 cut(s) 961
Sfr274I CTCGAG 1 cut(s) 842
Sfr303I CCGCGG 1 cut(s) 826
SgrBI CCGCGG 1 cut(s) 826
SinI GGWCC 1 cut(s) 1151
SlaI CTCGAG 1 cut(s) 842
SmaI CCCGGG 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 1376
SmlI CTYRAG 3 cut(s) 842, 1289, 1567
SmoI CTYRAG 3 cut(s) 842, 1289, 1567
Sse9I AATT 3 cut(s) 136, 1301, 1541
SseBI AGGCCT 1 cut(s) 289
SspDI GGCGCC 1 cut(s) 959
SspMI CTAG 5 cut(s) 5, 248, 1277, 1605, 1617
SstI GAGCTC 1 cut(s) 452
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 8 cut(s) 259, 260, 335, 434, 552, 711, 955, 1249
StyI CCWWGG 2 cut(s) 1063, 1332
TaaI ACNGT 5 cut(s) 72, 473, 542, 1048, 1472
TaiI ACGT 8 cut(s) 68, 619, 624, 674, 839, 969, 1203, 1350
TaqI TCGA 9 cut(s) 134, 204, 578, 699, 740, 843, 993, 1298, 1368
TasI AATT 3 cut(s) 136, 1301, 1541
TatI WGTACW 1 cut(s) 1020
TauI GCSGC 4 cut(s) 349, 365, 560, 1126
TfiI GAWTC 4 cut(s) 514, 580, 1324, 1579
Tru1I TTAA 5 cut(s) 83, 104, 150, 1101, 1630
Tru9I TTAA 5 cut(s) 83, 104, 150, 1101, 1630
TseFI GTSAC 4 cut(s) 66, 1048, 1091, 1380
TseI GCWGC 5 cut(s) 161, 211, 359, 1070, 1416
Tsp45I GTSAC 4 cut(s) 66, 1048, 1091, 1380
TspDTI ATGAA 2 cut(s) 538, 1249
TspGWI ACGGA 4 cut(s) 84, 105, 308, 976
TspMI CCCGGG 1 cut(s) 260
Tth111I GACNNNGTC 2 cut(s) 695, 809
Van91I CCANNNNNTGG 2 cut(s) 342, 1426
VpaK11BI GGWCC 1 cut(s) 1151
XagI CCTNNNNNAGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 1301
XceI RCATGY 1 cut(s) 1315
XcmI CCANNNNNNNNNTGG 1 cut(s) 1405
XhoI CTCGAG 1 cut(s) 842
XmaI CCCGGG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 1368
XspI CTAG 5 cut(s) 5, 248, 1277, 1605, 1617
ZraI GACGTC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.