pycom04g05880

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015060
Physical Location & Seq
Forward (+)
5734080 .. 5737890
3811 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1260 bp
ATGGATTCTAAAAATCACTATCCGAACTCATTGGGTCAGTGGGGTTGGATATGTAAATATCAAAAGCTCAGGCCCTACAAACAAATAAATAGGACCTTCTCCAAGCCCTTCGAAATAAACAGCAGCAGGGCAATTTTCCCTCCAAATCGAAGCAAGAAGGCAAAAAGAGGGCTGTTCGGGAGAGGGTTTTCAGTTCCAGTCCATCTTCTTCGTGAGCCGCCACGACCTCTCCTTCTCTGTCGCAGCCATCAGCAGACGGCAGCAGCAATGGCGTTGTCTTCTTCTTCGTCAGCCTACGACATGCCGTGGGTGGAGAAGTACAGGCCCACCAAGGTCGCCGACATTGTGGGCAACGAAGACGCCGTGTCCAGAATCCAGGTCATCGCTCGCGACGGCAACATGCCCAACCTCATCTTAGCCGGTCCTCCTGGAACTGGTAAGACCACTAGCATACTGGCTCTTGCACATGAGCTTTTGGGACCAAACTATAAGGAGGCTGTTTTAGAGCTGAATGCATCAGATGACAGGGGAATCGATGTTGTGAGGAACAAAATTAAAATGTTTGCTCAAAAGAAAGTTACACTGCCTTCCGGACGCCATAAAGTTGTCATATTGGATGAAGCTGACAGCATGACATCTGGAGCACAACAAGCTTTGAGGCGAACAATGGAAATATATTCAAATTCTACTCGTTTCGCTCTTGCGTGCAATACATCTTCCAAAATCATTGAGCCTATTCAGAGTAGATGTGCCCTTGTCCGGTTTTCTAGATTGACTGATGAGGAGATACTTGGTCGCCTAATGATTGTCGTTCAAGCTGAGAAGGTTCCATATGTTCCAGAAGGTCTTGAAGCCATTATTTTCACCGCTGATGGTGATATGAGGCAGGCACTGAATAACTTGCAAGCTACAAACAGTGGATTCCGGTTTGTGAACCAAGAAAATGTTTTCAAGGTCTGTGACCAGCCTCACCCATTGCATGTAAAGAATATGGTGCGCAACATACTGGAAGGGAAATTTGATGATGCTTGTTCGGGTCTCAAGCAGCTCTACGATCTGGGTTACTCCCCAACAGACATAATCACAACCTTTTTCCGTATCATAAAAAACTATGATATGGCTGAGTTTTTGAAATTGGAGTTCATGAAGGAAACTGGATTTGCTCACATGAGAATCTGTGATGGAGTTGGCTCGTACCTTCAGATGTGTGGTCTTCTGGCTAAGCTTTCCCTAGTTCGTGAAACCGCGAAGGAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000723 GO:0000731 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0030554 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031390 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033260 GO:0033554 GO:0033683 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0036094 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097367 GO:1900262 GO:1900264 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000112 GO:2000278 GO:2000573
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA Rep_fac_C

Protein Analysis

420

Amino Acids

47.04

Weight (kDa)

9.36

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
nSTAND_NTPase5 PF25199 121 - 239 3.5e-06 Novel STAND NTPase 5
AAA_14 PF13173 136 - 235 2.5e-06 AAA domain
AAA PF00004 137 - 254 8.6e-14 ATPase family associated with various cellular activities (AAA)
DNA_pol3_delta2 PF13177 177 - 258 2.4e-08 DNA polymerase III, delta subunit
Rep_fac_C PF08542 323 - 408 9.9e-24 Replication factor C C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63160
fragaria_vesca FvH4_5g01730 FvH4_5g01730
malus_domestica MD04G1065200.v1.1
prunus_persica Prupe.8G039600_v2.0.a1
pyrus_communis pycom04g05880
rosa_chinensis RchiOBHm_Chr7g0198991
rosa_laevigata RLG00000003826
rosa_multiflora Rmu_sc0006266.1_g000025 Rmu_sc0007328.1_g000009
rosa_roxburghii Rroxscaffold_3G00257230
rosa_rugosa Rorug07G0052000
rosa_samantha Rh7AG175000 Rh7BG178200 Rh7DG177600
rosa_wichuraiana Rw7G015350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 998
AccII CGCG 2 cut(s) 390, 1247
AccIII TCCGGA 1 cut(s) 590
AciI CCGC 3 cut(s) 218, 867, 1245
AcsI RAATTY 2 cut(s) 682, 1016
AcuI CTGAAG 1 cut(s) 1184
AcyI GRCGYC 2 cut(s) 360, 595
AfaI GTAC 2 cut(s) 320, 1196
AfiI CCNNNNNNNGG 2 cut(s) 434, 759
AgsI TTSAA 5 cut(s) 681, 815, 851, 952, 1132
AhdI GACNNNNNGTC 1 cut(s) 364
AjnI CCWGG 2 cut(s) 375, 427
AluBI AGCT 9 cut(s) 67, 472, 508, 623, 653, 818, 908, 1048, 1225
AluI AGCT 9 cut(s) 67, 472, 508, 623, 653, 818, 908, 1048, 1225
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 892
Aor13HI TCCGGA 1 cut(s) 590
AoxI GGCC 2 cut(s) 71, 323
ApeKI GCWGC 5 cut(s) 123, 243, 260, 263, 1045
ApoI RAATTY 2 cut(s) 682, 1016
AspLEI GCGC 1 cut(s) 999
AspS9I GGNCC 5 cut(s) 72, 93, 324, 422, 479
AsuHPI GGTGA 3 cut(s) 856, 887, 962
AsuII TTCGAA 1 cut(s) 111
AvaII GGWCC 3 cut(s) 93, 422, 479
BaeGI GKGCMC 1 cut(s) 754
BbsI GAAGAC 3 cut(s) 270, 363, 1205
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 5 cut(s) 135, 255, 272, 275, 1057
BccI CCATC 4 cut(s) 210, 255, 866, 1175
BceAI ACGGC 4 cut(s) 273, 289, 347, 409
BciT130I CCWGG 2 cut(s) 377, 429
BcoDI GTCTC 1 cut(s) 1043
BfaI CTAG 3 cut(s) 447, 768, 1232
BisI GCNGC 6 cut(s) 124, 218, 244, 261, 264, 1046
BlpI GCTNAGC 1 cut(s) 1221
BlsI GCNGC 6 cut(s) 125, 219, 245, 262, 265, 1047
Bme1390I CCNGG 2 cut(s) 377, 429
Bme18I GGWCC 3 cut(s) 93, 422, 479
BmeRI GACNNNNNGTC 1 cut(s) 364
BmgT120I GGNCC 5 cut(s) 72, 93, 324, 422, 479
BmiI GGNNCC 2 cut(s) 480, 828
BmrFI CCNGG 2 cut(s) 377, 429
BmsI GCATC 2 cut(s) 524, 1015
BpiI GAAGAC 3 cut(s) 270, 363, 1205
BpmI CTGGAG 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 68
Bpu1102I GCTNAGC 1 cut(s) 1221
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 1025
Bsa29I ATCGAT 1 cut(s) 534
BsaHI GRCGYC 2 cut(s) 360, 595
BsaI GGTCTC 1 cut(s) 1043
BsaJI CCNNGG 2 cut(s) 305, 330
BsaWI WCCGGW 3 cut(s) 590, 759, 924
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 759
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 5 cut(s) 197, 439, 459, 1011, 1159
Bse3DI GCAATG 2 cut(s) 273, 974
BseAI TCCGGA 1 cut(s) 590
BseBI CCWGG 2 cut(s) 377, 429
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 2 cut(s) 305, 330
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 2 cut(s) 434, 759
BseMI GCAATG 2 cut(s) 273, 974
BseMII CTCAG 3 cut(s) 82, 810, 1113
BseNI ACTGG 5 cut(s) 197, 439, 459, 1011, 1159
BseRI GAGGAG 1 cut(s) 797
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 5 cut(s) 135, 255, 272, 275, 1057
Bsh1236I CGCG 2 cut(s) 390, 1247
BshFI GGCC 2 cut(s) 73, 325
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 4 cut(s) 420, 591, 760, 925
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 2 cut(s) 434, 759
BsmAI GTCTC 1 cut(s) 1043
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 2 cut(s) 73, 325
Bso31I GGTCTC 1 cut(s) 1043
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 646, 754
Bsp13I TCCGGA 1 cut(s) 590
Bsp143I GATC 1 cut(s) 1054
Bsp1720I GCTNAGC 1 cut(s) 1221
Bsp68I TCGCGA 1 cut(s) 390
BspACI CCGC 3 cut(s) 218, 867, 1245
BspANI GGCC 2 cut(s) 73, 325
BspCNI CTCAG 3 cut(s) 81, 811, 1114
BspDI ATCGAT 1 cut(s) 534
BspEI TCCGGA 1 cut(s) 590
BspFNI CGCG 2 cut(s) 390, 1247
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 2 cut(s) 480, 828
BspT104I TTCGAA 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 1043
BsrDI GCAATG 2 cut(s) 273, 974
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 5 cut(s) 197, 439, 459, 1011, 1159
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 305, 330
BssMI GATC 1 cut(s) 1054
BssNI GRCGYC 2 cut(s) 360, 595
BssT1I CCWWGG 1 cut(s) 330
Bst2UI CCWGG 2 cut(s) 377, 429
Bst4CI ACNGT 1 cut(s) 917
BstACI GRCGYC 2 cut(s) 360, 595
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 4 cut(s) 388, 706, 888, 906
BstDEI CTNAG 5 cut(s) 68, 415, 819, 1122, 1221
BstDSI CCRYGG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 622
BstFNI CGCG 2 cut(s) 390, 1247
BstHHI GCGC 1 cut(s) 999
BstKTI GATC 1 cut(s) 1057
BstMAI GTCTC 1 cut(s) 1043
BstMBI GATC 1 cut(s) 1054
BstMWI GCNNNNNNNGC 2 cut(s) 269, 650
BstNI CCWGG 2 cut(s) 377, 429
BstNSI RCATGY 3 cut(s) 304, 403, 983
BstSCI CCNGG 2 cut(s) 375, 427
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 2 cut(s) 390, 1247
BstV1I GCAGC 5 cut(s) 135, 255, 272, 275, 1057
BstV2I GAAGAC 3 cut(s) 270, 363, 1205
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 2 cut(s) 73, 325
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 305
BtgZI GCGATG 1 cut(s) 367
BtsCI GGATG 1 cut(s) 622
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 4 cut(s) 44, 581, 890, 922
BtuMI TCGCGA 1 cut(s) 390
Cac8I GCNNGC 4 cut(s) 388, 706, 888, 906
CaiI CAGNNNCTG 1 cut(s) 892
CciI TCATGA 1 cut(s) 1143
CfoI GCGC 1 cut(s) 999
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 5 cut(s) 72, 93, 324, 422, 479
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 2 cut(s) 368, 603
Csp6I GTAC 2 cut(s) 319, 1195
CviAII CATG 7 cut(s) 301, 400, 467, 631, 980, 1144, 1168
CviQI GTAC 2 cut(s) 319, 1195
DdeI CTNAG 5 cut(s) 68, 415, 819, 1122, 1221
DpnI GATC 1 cut(s) 1056
DpnII GATC 1 cut(s) 1054
DriI GACNNNNNGTC 1 cut(s) 364
Eam1105I GACNNNNNGTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 1043
Eco47I GGWCC 3 cut(s) 93, 422, 479
Eco57I CTGAAG 1 cut(s) 1184
EcoO109I RGGNCCY 2 cut(s) 72, 93
EcoRII CCWGG 2 cut(s) 375, 427
EcoT14I CCWWGG 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 7 cut(s) 304, 403, 470, 634, 983, 1147, 1171
FaqI GGGAC 1 cut(s) 492
FatI CATG 7 cut(s) 300, 399, 466, 630, 979, 1143, 1167
FauNDI CATATG 1 cut(s) 832
Fnu4HI GCNGC 6 cut(s) 124, 218, 244, 261, 264, 1046
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 6 cut(s) 124, 218, 244, 261, 264, 1046
FspBI CTAG 3 cut(s) 447, 768, 1232
FspI TGCGCA 1 cut(s) 998
GlaI GCGC 1 cut(s) 998
GluI GCNGC 6 cut(s) 124, 218, 244, 261, 264, 1046
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 2 cut(s) 73, 325
HapII CCGG 4 cut(s) 420, 591, 760, 925
HgaI GACGC 2 cut(s) 368, 603
HhaI GCGC 1 cut(s) 999
Hin1I GRCGYC 2 cut(s) 360, 595
Hin1II CATG 7 cut(s) 304, 403, 470, 634, 983, 1147, 1171
Hin6I GCGC 1 cut(s) 997
HinP1I GCGC 1 cut(s) 997
HindIII AAGCTT 2 cut(s) 651, 1223
HinfI GANTC 5 cut(s) 5, 372, 531, 921, 1173
HpaII CCGG 4 cut(s) 420, 591, 760, 925
HphI GGTGA 3 cut(s) 856, 887, 962
Hpy166II GTNNAC 1 cut(s) 934
Hpy188I TCNGA 4 cut(s) 24, 520, 741, 1203
Hpy8I GTNNAC 1 cut(s) 934
Hpy99I CGWCG 1 cut(s) 395
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4V TGCA 5 cut(s) 464, 515, 708, 904, 979
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 650
HpyF3I CTNAG 5 cut(s) 68, 415, 819, 1122, 1221
Hsp92I GRCGYC 2 cut(s) 360, 595
Hsp92II CATG 7 cut(s) 304, 403, 470, 634, 983, 1147, 1171
HspAI GCGC 1 cut(s) 997
Kpn2I TCCGGA 1 cut(s) 590
Kzo9I GATC 1 cut(s) 1054
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 5 cut(s) 135, 255, 272, 275, 1057
LweI GCATC 2 cut(s) 524, 1015
MaeI CTAG 3 cut(s) 447, 768, 1232
MaeIII GTNAC 3 cut(s) 577, 959, 1061
MalI GATC 1 cut(s) 1056
MboI GATC 1 cut(s) 1054
MboII GAAGA 8 cut(s) 197, 200, 270, 273, 276, 368, 708, 1205
MhlI GDGCHC 2 cut(s) 646, 754
MluCI AATT 5 cut(s) 132, 552, 682, 1016, 1133
MmeI TCCRAC 1 cut(s) 26
Mph1103I ATGCAT 1 cut(s) 517
MroI TCCGGA 1 cut(s) 590
MseI TTAA 1 cut(s) 555
MspA1I CMGCKG 1 cut(s) 869
MspI CCGG 4 cut(s) 420, 591, 760, 925
MspR9I CCNGG 2 cut(s) 377, 429
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 2 cut(s) 377, 429
MvnI CGCG 2 cut(s) 390, 1247
MwoI GCNNNNNNNGC 2 cut(s) 269, 650
NdeI CATATG 1 cut(s) 832
NdeII GATC 1 cut(s) 1054
NlaIII CATG 7 cut(s) 304, 403, 470, 634, 983, 1147, 1171
NlaIV GGNNCC 2 cut(s) 480, 828
NmuCI GTSAC 1 cut(s) 959
NruI TCGCGA 1 cut(s) 390
NsbI TGCGCA 1 cut(s) 998
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 3 cut(s) 304, 403, 983
NspV TTCGAA 1 cut(s) 111
PagI TCATGA 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 5 cut(s) 5, 372, 531, 921, 1173
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 6 cut(s) 125, 219, 245, 262, 265, 1047
PpuMI RGGWCCY 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 93
Psp6I CCWGG 2 cut(s) 375, 427
PspGI CCWGG 2 cut(s) 375, 427
PspN4I GGNNCC 2 cut(s) 480, 828
PspPI GGNCC 5 cut(s) 72, 93, 324, 422, 479
PspPPI RGGWCCY 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 892
RruI TCGCGA 1 cut(s) 390
RsaI GTAC 2 cut(s) 320, 1196
RsaNI GTAC 2 cut(s) 319, 1195
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 6 cut(s) 124, 218, 244, 261, 264, 1046
Sau3AI GATC 1 cut(s) 1054
Sau96I GGNCC 5 cut(s) 72, 93, 324, 422, 479
ScrFI CCNGG 2 cut(s) 377, 429
SduI GDGCHC 2 cut(s) 646, 754
SfaNI GCATC 2 cut(s) 524, 1015
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 3 cut(s) 93, 422, 479
SmlI CTYRAG 1 cut(s) 1040
SmoI CTYRAG 1 cut(s) 1040
Sse9I AATT 5 cut(s) 132, 552, 682, 1016, 1133
SsiI CCGC 3 cut(s) 218, 867, 1245
SspMI CTAG 3 cut(s) 447, 768, 1232
StyD4I CCNGG 2 cut(s) 375, 427
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 917
TaqI TCGA 3 cut(s) 111, 148, 534
TasI AATT 5 cut(s) 132, 552, 682, 1016, 1133
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 5 cut(s) 5, 372, 531, 921, 1173
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 4 cut(s) 44, 588, 897, 922
TseFI GTSAC 1 cut(s) 959
TseI GCWGC 5 cut(s) 123, 243, 260, 263, 1045
Tsp45I GTSAC 1 cut(s) 959
TspDTI ATGAA 3 cut(s) 633, 1132, 1160
TspGWI ACGGA 1 cut(s) 1085
TspRI CASTG 4 cut(s) 44, 588, 897, 922
VpaK11BI GGWCC 3 cut(s) 93, 422, 479
XapI RAATTY 2 cut(s) 682, 1016
XbaI TCTAGA 1 cut(s) 767
XceI RCATGY 3 cut(s) 304, 403, 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XspI CTAG 3 cut(s) 447, 768, 1232
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.