pycom05g04660

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008325
Physical Location & Seq
Reverse (-)
5491109 .. 5492989
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
ATGGCTTCTGCATCTTTCCTCAAGTCTTCTCCGGTTCTCGACAAGTCTGAGTGGGTGAAGGGCCAGACCCTCCGCCAGCCTTCCGCCTCCTCGGTCGTCAGATGCCTACCCACTGCCCCAACCGGTCTAACAATTCGTGCCGGTTCCTATGCTGATGAGCTTGTTAAGACTGCTAAAACTGTTGCATCCCCAGGCCGTGGAATCTTGGCCATGGATGAGTCCAATGCAACCTGTGGCAAGCGTCTTGCCTCAATCGGGCTAGAGAACACTGAGGCCAACCGCCAAGCCTACCGTACCCTCCTTGTGAGCGCCCCTGGCCTTGGCCAGTACGTTTCAGGTGCCATTCTCTTTGAGGAGACTCTCTACCAATCCACTGTTGATGGAAAGAAGATCGTTGATGTGCTCATTGAGCAAGGCATCGTCCCCGGGATTAAAGTCGACAAGGGTTTGGTGCCCCTAACTGGTTCCAACGATGAGTCCTGGTGCCAAGGACTTGATGGTCTTGCCTCTCGCACAGCTGCCTACTACCAGCAGGGAGCTCGTTTCGCCAAATGGCGTACAGTTGTGAGCATCCCCAATGGCCCATCAGCTCTTGCAGTAAAGGAAGCAGCATGGGGTCTCGCTCGCTACGCTTCCATTGCACAAGACAGTGGTTTGGTCCCAATTGTTGAGCCAGAGATCTTGCTTGACGGTGAACACACCATTGAAAGGACTTTCGAAGTCGCCTTGAAGGTGTGGGCCGAGGTTTTCTTCTACCTAGCCGAGAACAATGTCTTGTTCGAGGGTATCCTCCTCAAGCCAAGTATGGTTACTCCTGGTGCAGAGGCCAAGGAAAGAGCCACCCCTCAACAGGTTGCGGACTACACCCTCAAGCTCCTCCACAGGAGAATCCCCCCAGCTGTGCCCGGGATCATGTTTTTGTCCGGTGGACAATCTGAGGTCGAGGCAACCCTGAACTTGAACGCGATGAACCAATCGCCAAACCCATGGCATGTGTCGTTCTCCTACGCCAGAGCTCTCCAAAACACCTGTTTGAAGATATGGGGAGGCAGACCCGAGAACGTGAAGGCAGCTCAGGATGCTCTGCTCATCCGTGCCAAGGCCAACTCACTCGCACAGCTTGGCAAGTACACTGGAGAAGGAGAGTCCGAGGAGGCAAAGAAGGAATTGTTCGTCAAGGGCTATGTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

398

Amino Acids

42.82

Weight (kDa)

7.6

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 53 - 397 1.4e-157 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 498
AccB1I GGYRCC 3 cut(s) 338, 451, 483
AccB7I CCANNNNNTGG 1 cut(s) 197
AccI GTMKAC 1 cut(s) 438
AccII CGCG 1 cut(s) 965
AciI CCGC 4 cut(s) 73, 84, 280, 857
AclWI GGATC 1 cut(s) 917
AcoI YGGCCR 2 cut(s) 207, 322
AfaI GTAC 5 cut(s) 295, 329, 559, 1130, 1190
AfiI CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 4 cut(s) 707, 730, 961, 1036
AjnI CCWGG 4 cut(s) 190, 313, 479, 814
AluBI AGCT 9 cut(s) 160, 518, 539, 590, 874, 899, 1016, 1073, 1120
AluI AGCT 9 cut(s) 160, 518, 539, 590, 874, 899, 1016, 1073, 1120
Alw21I GWGCWC 3 cut(s) 405, 541, 1018
Alw26I GTCTC 2 cut(s) 350, 623
AlwI GGATC 1 cut(s) 917
Ama87I CYCGRG 3 cut(s) 425, 905, 1055
ApeKI GCWGC 3 cut(s) 518, 608, 1070
AsiGI ACCGGT 1 cut(s) 122
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 4 cut(s) 61, 581, 658, 738
AsuC2I CCSGG 4 cut(s) 426, 427, 906, 907
AsuHPI GGTGA 2 cut(s) 67, 704
AsuII TTCGAA 1 cut(s) 717
AvaI CYCGRG 3 cut(s) 425, 905, 1055
AvaII GGWCC 1 cut(s) 658
BaeGI GKGCMC 2 cut(s) 456, 906
BalI TGGCCA 2 cut(s) 209, 324
BanI GGYRCC 3 cut(s) 338, 451, 483
BanII GRGCYC 2 cut(s) 541, 1018
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 3 cut(s) 405, 541, 1018
BbvI GCAGC 3 cut(s) 505, 620, 1082
BccI CCATC 3 cut(s) 374, 491, 592
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 4 cut(s) 192, 315, 481, 816
BciVI GTATCC 1 cut(s) 797
BcnI CCSGG 4 cut(s) 426, 427, 906, 907
BcoDI GTCTC 2 cut(s) 350, 623
BfaI CTAG 2 cut(s) 260, 758
BfoI RGCGCY 1 cut(s) 312
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 1 cut(s) 678
BisI GCNGC 3 cut(s) 519, 609, 1071
BlsI GCNGC 3 cut(s) 520, 610, 1072
Bme1390I CCNGG 8 cut(s) 192, 315, 426, 427, 481, 816, 906, 907
Bme18I GGWCC 1 cut(s) 658
BmeT110I CYCGRG 3 cut(s) 425, 905, 1055
BmgT120I GGNCC 4 cut(s) 61, 581, 658, 738
BmiI GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
BmrFI CCNGG 8 cut(s) 192, 315, 426, 427, 481, 816, 906, 907
BmsI GCATC 6 cut(s) 20, 92, 194, 426, 579, 1069
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 1155
Bpu10I CCTNAGC 1 cut(s) 1074
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 3 cut(s) 5, 779, 854
BpuMI CCSGG 4 cut(s) 426, 427, 906, 907
BsaI GGTCTC 1 cut(s) 623
BsaWI WCCGGW 3 cut(s) 31, 122, 923
Bsc4I CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
Bse118I RCCGGY 2 cut(s) 122, 140
Bse1I ACTGG 3 cut(s) 325, 466, 1138
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 4 cut(s) 192, 315, 481, 816
BseGI GGATG 5 cut(s) 185, 220, 570, 1084, 1089
BseLI CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 4 cut(s) 39, 261, 927, 1088
BseNI ACTGG 3 cut(s) 325, 466, 1138
BseRI GAGGAG 5 cut(s) 79, 368, 782, 866, 1166
BseSI GKGCMC 2 cut(s) 456, 906
BseXI GCAGC 3 cut(s) 505, 620, 1082
BseYI CCCAGC 1 cut(s) 895
BsgI GTGCAG 1 cut(s) 840
Bsh1236I CGCG 1 cut(s) 965
Bsh1285I CGRYCG 1 cut(s) 96
BshNI GGYRCC 3 cut(s) 338, 451, 483
BshTI ACCGGT 1 cut(s) 122
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 3 cut(s) 405, 541, 1018
BsiHKCI CYCGRG 3 cut(s) 425, 905, 1055
BsiSI CCGG 6 cut(s) 32, 123, 141, 426, 906, 924
BslFI GGGAC 2 cut(s) 407, 644
BslI CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
BsmAI GTCTC 2 cut(s) 350, 623
BsmFI GGGAC 2 cut(s) 407, 644
Bso31I GGTCTC 1 cut(s) 623
BsoBI CYCGRG 3 cut(s) 425, 905, 1055
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 5 cut(s) 405, 456, 541, 906, 1018
Bsp143I GATC 3 cut(s) 390, 678, 909
Bsp19I CCATGG 2 cut(s) 210, 986
BspACI CCGC 4 cut(s) 73, 84, 280, 857
BspCNI CTCAG 4 cut(s) 40, 262, 928, 1087
BspFNI CGCG 1 cut(s) 965
BspLI GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
BspPI GGATC 1 cut(s) 917
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 3 cut(s) 338, 451, 483
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 2 cut(s) 122, 140
BsrI ACTGG 3 cut(s) 325, 466, 1138
BssAI RCCGGY 2 cut(s) 122, 140
BssMI GATC 3 cut(s) 390, 678, 909
BssT1I CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
Bst2UI CCWGG 4 cut(s) 192, 315, 481, 816
Bst4CI ACNGT 6 cut(s) 181, 293, 376, 562, 650, 692
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 3 cut(s) 77, 239, 625
BstDEI CTNAG 4 cut(s) 48, 270, 936, 1074
BstDSI CCRYGG 3 cut(s) 196, 210, 986
BstF5I GGATG 5 cut(s) 185, 220, 570, 1084, 1089
BstFNI CGCG 1 cut(s) 965
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 3 cut(s) 393, 681, 912
BstMAI GTCTC 2 cut(s) 350, 623
BstMBI GATC 3 cut(s) 390, 678, 909
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 6 cut(s) 315, 409, 545, 629, 638, 1079
BstNI CCWGG 4 cut(s) 192, 315, 481, 816
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 8 cut(s) 190, 313, 424, 425, 479, 814, 904, 905
BstSLI GKGCMC 2 cut(s) 456, 906
BstUI CGCG 1 cut(s) 965
BstV1I GCAGC 3 cut(s) 505, 620, 1082
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 1 cut(s) 678
BsuI GTATCC 1 cut(s) 797
BtgI CCRYGG 3 cut(s) 196, 210, 986
BtgZI GCGATG 1 cut(s) 980
BtsCI GGATG 5 cut(s) 185, 220, 570, 1084, 1089
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 5 cut(s) 111, 267, 372, 655, 1131
Cac8I GCNNGC 3 cut(s) 77, 239, 625
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 2 cut(s) 122, 140
Cfr13I GGNCC 4 cut(s) 61, 581, 658, 738
Cfr9I CCCGGG 2 cut(s) 425, 905
CseI GACGC 1 cut(s) 230
Csp6I GTAC 5 cut(s) 294, 328, 558, 1129, 1189
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 5 cut(s) 211, 612, 913, 987, 992
CviQI GTAC 5 cut(s) 294, 328, 558, 1129, 1189
DdeI CTNAG 4 cut(s) 48, 270, 936, 1074
DpnI GATC 3 cut(s) 392, 680, 911
DpnII GATC 3 cut(s) 390, 678, 909
DrdI GACNNNNNNGTC 1 cut(s) 498
DseDI GACNNNNNNGTC 1 cut(s) 498
EaeI YGGCCR 2 cut(s) 207, 322
EciI GGCGGA 2 cut(s) 62, 73
Ecl136II GAGCTC 2 cut(s) 539, 1016
Eco130I CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
Eco24I GRGCYC 2 cut(s) 541, 1018
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 658
Eco53kI GAGCTC 2 cut(s) 539, 1016
Eco88I CYCGRG 3 cut(s) 425, 905, 1055
EcoICRI GAGCTC 2 cut(s) 539, 1016
EcoRII CCWGG 4 cut(s) 190, 313, 479, 814
EcoT14I CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
EcoT38I GRGCYC 2 cut(s) 541, 1018
ErhI CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
FaeI CATG 5 cut(s) 214, 615, 916, 990, 995
FaiI YATR 9 cut(s) 150, 212, 613, 806, 914, 988, 993, 1042, 1185
FaqI GGGAC 2 cut(s) 407, 644
FatI CATG 5 cut(s) 210, 611, 912, 986, 991
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 519, 609, 1071
FokI GGATG 5 cut(s) 172, 227, 557, 1076, 1091
FriOI GRGCYC 2 cut(s) 541, 1018
Fsp4HI GCNGC 3 cut(s) 519, 609, 1071
FspBI CTAG 2 cut(s) 260, 758
GlaI GCGC 1 cut(s) 310
GluI GCNGC 3 cut(s) 519, 609, 1071
GsaI CCCAGC 1 cut(s) 899
GsuI CTGGAG 1 cut(s) 1155
HaeII RGCGCY 1 cut(s) 312
HapII CCGG 6 cut(s) 32, 123, 141, 426, 906, 924
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 311
Hin1II CATG 5 cut(s) 214, 615, 916, 990, 995
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 6 cut(s) 201, 218, 358, 476, 888, 1145
HpaII CCGG 6 cut(s) 32, 123, 141, 426, 906, 924
HphI GGTGA 2 cut(s) 67, 704
Hpy166II GTNNAC 5 cut(s) 439, 695, 929, 1131, 1189
Hpy188I TCNGA 4 cut(s) 49, 101, 937, 1150
Hpy188III TCNNGA 2 cut(s) 38, 1076
Hpy8I GTNNAC 5 cut(s) 439, 695, 929, 1131, 1189
HpyAV CCTTC 6 cut(s) 52, 90, 724, 1060, 1133, 1156
HpyCH4III ACNGT 6 cut(s) 181, 293, 376, 562, 650, 692
HpyCH4IV ACGT 2 cut(s) 330, 1062
HpyCH4V TGCA 6 cut(s) 11, 185, 227, 596, 641, 821
HpyF10VI GCNNNNNNNGC 6 cut(s) 315, 409, 545, 629, 638, 1079
HpyF3I CTNAG 4 cut(s) 48, 270, 936, 1074
HpySE526I ACGT 2 cut(s) 330, 1062
Hsp92II CATG 5 cut(s) 214, 615, 916, 990, 995
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 3 cut(s) 390, 678, 909
LmnI GCTCC 2 cut(s) 536, 879
Lsp1109I GCAGC 3 cut(s) 505, 620, 1082
LweI GCATC 6 cut(s) 20, 92, 194, 426, 579, 1069
MaeI CTAG 2 cut(s) 260, 758
MaeII ACGT 2 cut(s) 330, 1062
MaeIII GTNAC 1 cut(s) 808
MalI GATC 3 cut(s) 392, 680, 911
MboI GATC 3 cut(s) 390, 678, 909
MboII GAAGA 4 cut(s) 18, 400, 742, 1048
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 5 cut(s) 405, 456, 541, 906, 1018
MlsI TGGCCA 2 cut(s) 209, 324
MluCI AATT 3 cut(s) 132, 663, 1166
MluNI TGGCCA 2 cut(s) 209, 324
MlyI GAGTC 4 cut(s) 227, 352, 485, 1154
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 2 cut(s) 209, 324
MscI TGGCCA 2 cut(s) 209, 324
MseI TTAA 2 cut(s) 165, 432
Msp20I TGGCCA 2 cut(s) 209, 324
MspA1I CMGCKG 2 cut(s) 518, 899
MspI CCGG 6 cut(s) 32, 123, 141, 426, 906, 924
MspR9I CCNGG 8 cut(s) 192, 315, 426, 427, 481, 816, 906, 907
MunI CAATTG 1 cut(s) 663
MvaI CCWGG 4 cut(s) 192, 315, 481, 816
MvnI CGCG 1 cut(s) 965
MwoI GCNNNNNNNGC 6 cut(s) 315, 409, 545, 629, 638, 1079
NciI CCSGG 4 cut(s) 426, 427, 906, 907
NcoI CCATGG 2 cut(s) 210, 986
NdeII GATC 3 cut(s) 390, 678, 909
NlaIII CATG 5 cut(s) 214, 615, 916, 990, 995
NlaIV GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
NmeAIII GCCGAG 2 cut(s) 766, 787
NspI RCATGY 1 cut(s) 995
NspV TTCGAA 1 cut(s) 717
PfeI GAWTC 2 cut(s) 201, 888
PflMI CCANNNNNTGG 1 cut(s) 197
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 3 cut(s) 520, 610, 1072
PleI GAGTC 4 cut(s) 226, 352, 484, 1153
PpsI GAGTC 4 cut(s) 226, 352, 484, 1153
Psp124BI GAGCTC 2 cut(s) 541, 1018
Psp6I CCWGG 4 cut(s) 190, 313, 479, 814
PspFI CCCAGC 1 cut(s) 895
PspGI CCWGG 4 cut(s) 190, 313, 479, 814
PspN4I GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
PspPI GGNCC 4 cut(s) 61, 581, 658, 738
PsuI RGATCY 1 cut(s) 678
PvuII CAGCTG 2 cut(s) 518, 899
RsaI GTAC 5 cut(s) 295, 329, 559, 1130, 1190
RsaNI GTAC 5 cut(s) 294, 328, 558, 1129, 1189
SacI GAGCTC 2 cut(s) 541, 1018
SalI GTCGAC 1 cut(s) 437
SaqAI TTAA 2 cut(s) 165, 432
SatI GCNGC 3 cut(s) 519, 609, 1071
Sau3AI GATC 3 cut(s) 390, 678, 909
Sau96I GGNCC 4 cut(s) 61, 581, 658, 738
SchI GAGTC 4 cut(s) 227, 352, 485, 1154
ScrFI CCNGG 8 cut(s) 192, 315, 426, 427, 481, 816, 906, 907
SduI GDGCHC 5 cut(s) 405, 456, 541, 906, 1018
SfaNI GCATC 6 cut(s) 20, 92, 194, 426, 579, 1069
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 1 cut(s) 658
SmaI CCCGGG 2 cut(s) 427, 907
SmlI CTYRAG 3 cut(s) 20, 794, 869
SmoI CTYRAG 3 cut(s) 20, 794, 869
Sse9I AATT 3 cut(s) 132, 663, 1166
SsiI CCGC 4 cut(s) 73, 84, 280, 857
SspMI CTAG 2 cut(s) 260, 758
SstI GAGCTC 2 cut(s) 541, 1018
StyD4I CCNGG 8 cut(s) 190, 313, 424, 425, 479, 814, 904, 905
StyI CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
TaaI ACNGT 6 cut(s) 181, 293, 376, 562, 650, 692
TaiI ACGT 2 cut(s) 333, 1065
TaqI TCGA 5 cut(s) 39, 438, 717, 780, 942
TaqII GACCGA 1 cut(s) 82
TasI AATT 3 cut(s) 132, 663, 1166
TatI WGTACW 2 cut(s) 1128, 1188
TfiI GAWTC 2 cut(s) 201, 888
Tru1I TTAA 2 cut(s) 165, 432
Tru9I TTAA 2 cut(s) 165, 432
TscAI CASTG 5 cut(s) 118, 274, 379, 655, 1138
TseI GCWGC 3 cut(s) 518, 608, 1070
TspDTI ATGAA 1 cut(s) 983
TspGWI ACGGA 1 cut(s) 1082
TspMI CCCGGG 2 cut(s) 425, 905
TspRI CASTG 5 cut(s) 118, 274, 379, 655, 1138
Van91I CCANNNNNTGG 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 658
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmaI CCCGGG 2 cut(s) 425, 905
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 2 cut(s) 260, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.