pycom05g06190

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009094
Physical Location & Seq
Forward (+)
8113874 .. 8116624
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGGCGATCACTCTCCACTTCGGCCGAATTCTACCGCGTAACGATCTCTTCCTCTCAGAGAGCCTCGCCGGGAGGGTATCTCTCAGTTGCCGGAAACCTTTCACCGTCCGATGCTCCGGCGACTCCTCTTCCTTCGCCGTCGAGTCCGACTTCGACGCCAAGGTCTTCCGCAAGAACCTCACTCGAAGCAAGAACTACAACCGCAGAGGCTTCGGCCACAAAGAGGTGACATTGGAGCTCATGAACCGCGAGTACACAAGTGAGAAAGCTGAGAACGGGTTCGAGTACACCTGGGGAAACGTGACGGTGAAGCTCGCGGAGGCGTACGGATTCTGTTGGGGCGTTGAGAGAGCCGTGCAGATTGCTTACGAGGCCAGAAAGCAGTTTCCCGATGATAAGATTTGGATCACCAACGAAATCATCCACAATCCAACGGTCAATAAGAGGTTGGAGGAGATGAAGGTGGAGAACATTCCGATAGAGGAAGGTCAGAAGCAGTTTGAGGTGGTGAACAAGGACGATGTGGTCATTTTGCCTGCTTTCGGAGCCGGAGTGGATGAGATGCTCGACTTGAGTAACAGAGATGTTCAAATTGTTGACACTACTTGCCCCTGGGTTTCCAAGGTTTGGAATACTGTGGAGAAGCACAAGAAAGGAGATTACACTTCGATCATTCATGGGAAATATTCTCATGAGGAGACTGTGGCCACTGCTTCTTTTGCCGGGAAGTACGTTATAGTGAAAAACATGGATGAGGCAACGTATGTGTGTGACTATATTCTTGGCGGGGAACTTAATGGGTCTAGCTCAACTAAAGAGGCGTTTATGGAGAAATTTAAAAAGGCACTCTCTCCGGGGTTTGATCCAGACAGAGACCTGGTAAAAGTTGGGATTGCAAATCAGACAACAATGCTTAAGGGGGAAACGGAAGAGATTGGAAAATTAGTAGAGAAGACCATGATGAGTAAGCACGGAGTGGAAAATATAAATGACCACTTTGTGAGCTTCAACACAATATGTGATGCAACTCAGGAGCGACAAGATGCAATGTACAAGCTCGTTGAGGAAAAGCTAGATCTTTTGTTAGTCATTGGCGGGTGGAACTCAAGTAACACCTCTCACCTACAAGAGATTGCAGAAGAACGCGGAATTCCCTCGTATTGGATTGATAGTGAGAAGAGAATAGGCCCTGGAAACAGAATAGCTTATAAGCTCAATCATGGCGAGTTGGTCGAGAAAGAAAACTGGCTGCCAGAAGGTCCTGTCACTATTGGAGTGACATCTGGGGCATCTACTCCCGATAAGGTTGTTGAAGACGCTCTTATCAGGGTGTTCGACCTCAAGCGTGAAGAAGTCCTGCAACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
LYTB

Protein Analysis

457

Amino Acids

51.5

Weight (kDa)

5.37

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LYTB PF02401 103 - 440 3.5e-80 LytB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1209
AasI GACNNNNNNGTC 2 cut(s) 161, 524
AccB7I CCANNNNNTGG 1 cut(s) 627
AccII CGCG 4 cut(s) 37, 249, 317, 1146
AciI CCGC 8 cut(s) 35, 169, 202, 247, 317, 786, 1095, 1146
AclWI GGATC 2 cut(s) 413, 857
AcoI YGGCCR 3 cut(s) 22, 214, 705
AcsI RAATTY 3 cut(s) 27, 833, 1149
AcyI GRCGYC 1 cut(s) 156
AdeI CACNNNGTG 2 cut(s) 976, 1000
AfaI GTAC 5 cut(s) 254, 287, 326, 731, 1052
AfiI CCNNNNNNNGG 4 cut(s) 223, 542, 627, 1161
AflII CTTAAG 1 cut(s) 914
AgsI TTSAA 3 cut(s) 590, 1009, 1313
AjnI CCWGG 4 cut(s) 290, 611, 876, 1189
AluBI AGCT 9 cut(s) 238, 269, 313, 807, 1005, 1057, 1072, 1205, 1213
AluI AGCT 9 cut(s) 238, 269, 313, 807, 1005, 1057, 1072, 1205, 1213
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 2 cut(s) 692, 867
AlwI GGATC 2 cut(s) 413, 857
AoxI GGCC 5 cut(s) 22, 214, 372, 705, 1186
ApeKI GCWGC 1 cut(s) 1249
ApoI RAATTY 3 cut(s) 27, 833, 1149
Asp700I GAANNNNTTC 2 cut(s) 98, 278
AspS9I GGNCC 2 cut(s) 1187, 1259
AsuC2I CCSGG 3 cut(s) 70, 724, 855
AsuHPI GGTGA 6 cut(s) 94, 238, 319, 400, 520, 1112
AvaII GGWCC 1 cut(s) 1259
BalI TGGCCA 1 cut(s) 707
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 3 cut(s) 157, 959, 1320
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 1236
BceAI ACGGC 2 cut(s) 122, 338
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 4 cut(s) 292, 613, 878, 1191
BcnI CCSGG 3 cut(s) 70, 724, 855
BcoDI GTCTC 2 cut(s) 692, 867
BfaI CTAG 3 cut(s) 804, 1073, 1364
BfrI CTTAAG 1 cut(s) 914
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 1 cut(s) 1250
BlsI GCNGC 1 cut(s) 1251
Bme1390I CCNGG 7 cut(s) 70, 292, 613, 724, 855, 878, 1191
Bme18I GGWCC 1 cut(s) 1259
BmgT120I GGNCC 2 cut(s) 1187, 1259
BmiI GGNNCC 1 cut(s) 547
BmrFI CCNGG 7 cut(s) 70, 292, 613, 724, 855, 878, 1191
BmsI GCATC 5 cut(s) 101, 552, 1012, 1033, 1298
BpiI GAAGAC 3 cut(s) 157, 959, 1320
BplI GAGNNNNNCTC 2 cut(s) 64, 96
BpuEI CTTGAG 3 cut(s) 592, 1090, 1325
BpuMI CCSGG 3 cut(s) 70, 724, 855
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 867
BsaJI CCNNGG 7 cut(s) 159, 291, 611, 612, 621, 854, 1189
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 542, 627, 1161
Bse1I ACTGG 1 cut(s) 1250
Bse3DI GCAATG 1 cut(s) 1053
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 4 cut(s) 292, 613, 878, 1191
BseDI CCNNGG 7 cut(s) 159, 291, 611, 612, 621, 854, 1189
BseGI GGATG 3 cut(s) 420, 562, 757
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 4 cut(s) 223, 542, 627, 1161
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 4 cut(s) 69, 97, 261, 1043
BseNI ACTGG 1 cut(s) 1250
BseRI GAGGAG 3 cut(s) 115, 467, 710
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 1236
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 4 cut(s) 37, 249, 317, 1146
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 24, 216, 374, 707, 1188
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 6 cut(s) 69, 91, 117, 549, 723, 854
BsiWI CGTACG 1 cut(s) 324
BslI CCNNNNNNNGG 4 cut(s) 223, 542, 627, 1161
BsmAI GTCTC 2 cut(s) 692, 867
BsnI GGCC 5 cut(s) 24, 216, 374, 707, 1188
Bso31I GGTCTC 1 cut(s) 867
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
BspACI CCGC 8 cut(s) 35, 169, 202, 247, 317, 786, 1095, 1146
BspANI GGCC 5 cut(s) 24, 216, 374, 707, 1188
BspCNI CTCAG 4 cut(s) 68, 96, 262, 1042
BspFNI CGCG 4 cut(s) 37, 249, 317, 1146
BspHI TCATGA 2 cut(s) 240, 691
BspLI GGNNCC 1 cut(s) 547
BspPI GGATC 2 cut(s) 413, 857
BspTI CTTAAG 1 cut(s) 914
BspTNI GGTCTC 1 cut(s) 867
BsrDI GCAATG 1 cut(s) 1053
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 1 cut(s) 1250
BssECI CCNNGG 7 cut(s) 159, 291, 611, 612, 621, 854, 1189
BssMI GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 159, 621
Bst2UI CCWGG 4 cut(s) 292, 613, 878, 1191
Bst4CI ACNGT 5 cut(s) 106, 307, 436, 637, 703
Bst6I CTCTTC 4 cut(s) 53, 133, 924, 1172
BstACI GRCGYC 1 cut(s) 156
BstAFI CTTAAG 1 cut(s) 914
BstAUI TGTACA 1 cut(s) 1050
BstC8I GCNNGC 2 cut(s) 315, 537
BstDEI CTNAG 4 cut(s) 55, 83, 270, 1029
BstF5I GGATG 3 cut(s) 420, 562, 757
BstFNI CGCG 4 cut(s) 37, 249, 317, 1146
BstKTI GATC 6 cut(s) 9, 46, 408, 672, 865, 1078
BstMAI GTCTC 2 cut(s) 692, 867
BstMBI GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 371, 545, 719
BstNI CCWGG 4 cut(s) 292, 613, 878, 1191
BstSCI CCNGG 7 cut(s) 68, 290, 611, 722, 853, 876, 1189
BstUI CGCG 4 cut(s) 37, 249, 317, 1146
BstV1I GCAGC 1 cut(s) 1236
BstV2I GAAGAC 3 cut(s) 157, 959, 1320
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 5 cut(s) 24, 216, 374, 707, 1188
BtsCI GGATG 3 cut(s) 420, 562, 757
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 1 cut(s) 708
Cac8I GCNNGC 2 cut(s) 315, 537
CciI TCATGA 2 cut(s) 240, 691
Cfr13I GGNCC 2 cut(s) 1187, 1259
CseI GACGC 2 cut(s) 164, 1325
CsiI ACCWGGT 1 cut(s) 876
Csp6I GTAC 5 cut(s) 253, 286, 325, 730, 1051
CviAII CATG 6 cut(s) 241, 677, 692, 748, 958, 1220
CviQI GTAC 5 cut(s) 253, 286, 325, 730, 1051
DdeI CTNAG 4 cut(s) 55, 83, 270, 1029
DpnI GATC 6 cut(s) 8, 45, 407, 671, 864, 1077
DpnII GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
DraI TTTAAA 1 cut(s) 838
DraIII CACNNNGTG 2 cut(s) 976, 1000
DrdI GACNNNNNNGTC 2 cut(s) 161, 524
DseDI GACNNNNNNGTC 2 cut(s) 161, 524
EaeI YGGCCR 3 cut(s) 22, 214, 705
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 4 cut(s) 53, 133, 924, 1172
EarI CTCTTC 4 cut(s) 53, 133, 924, 1172
Ecl136II GAGCTC 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 159, 621
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 1259
Eco52I CGGCCG 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 2 cut(s) 1187, 1259
EcoRI GAATTC 2 cut(s) 27, 1149
EcoRII CCWGG 4 cut(s) 290, 611, 876, 1189
EcoT14I CCWWGG 2 cut(s) 159, 621
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 2 cut(s) 159, 621
FaeI CATG 6 cut(s) 244, 680, 695, 751, 961, 1223
FalI AAGNNNNNCTT 2 cut(s) 1305, 1337
FatI CATG 6 cut(s) 240, 676, 691, 747, 957, 1219
FauI CCCGC 2 cut(s) 779, 1088
Fnu4HI GCNGC 1 cut(s) 1250
FokI GGATG 3 cut(s) 407, 569, 764
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 1250
FspBI CTAG 3 cut(s) 804, 1073, 1364
GluI GCNGC 1 cut(s) 1250
HaeIII GGCC 5 cut(s) 24, 216, 374, 707, 1188
HapII CCGG 6 cut(s) 69, 91, 117, 549, 723, 854
HgaI GACGC 2 cut(s) 164, 1325
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 6 cut(s) 244, 680, 695, 751, 961, 1223
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 3 cut(s) 122, 143, 330
HpaII CCGG 6 cut(s) 69, 91, 117, 549, 723, 854
HphI GGTGA 6 cut(s) 94, 238, 319, 400, 520, 1112
Hpy166II GTNNAC 4 cut(s) 255, 288, 511, 598
Hpy188I TCNGA 7 cut(s) 58, 110, 148, 477, 492, 545, 903
Hpy188III TCNNGA 7 cut(s) 241, 389, 692, 866, 1031, 1234, 1298
Hpy8I GTNNAC 4 cut(s) 255, 288, 511, 598
Hpy99I CGWCG 2 cut(s) 143, 158
HpyAV CCTTC 4 cut(s) 142, 454, 479, 1250
HpyCH4III ACNGT 5 cut(s) 106, 307, 436, 637, 703
HpyCH4IV ACGT 3 cut(s) 300, 732, 761
HpyCH4V TGCA 6 cut(s) 358, 896, 1025, 1046, 1136, 1360
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 545, 719
HpyF3I CTNAG 4 cut(s) 55, 83, 270, 1029
HpySE526I ACGT 3 cut(s) 300, 732, 761
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 6 cut(s) 244, 680, 695, 751, 961, 1223
Kzo9I GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
LmnI GCTCC 4 cut(s) 119, 235, 545, 1033
Lsp1109I GCAGC 1 cut(s) 1236
LweI GCATC 5 cut(s) 101, 552, 1012, 1033, 1298
MabI ACCWGGT 1 cut(s) 876
MaeI CTAG 3 cut(s) 804, 1073, 1364
MaeII ACGT 3 cut(s) 300, 732, 761
MaeIII GTNAC 8 cut(s) 38, 226, 301, 575, 770, 1109, 1264, 1276
MalI GATC 6 cut(s) 8, 45, 407, 671, 864, 1077
MboI GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
MboII GAAGA 9 cut(s) 40, 120, 157, 941, 964, 1151, 1189, 1325, 1361
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 707
MluCI AATT 5 cut(s) 27, 591, 833, 941, 1149
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 2 cut(s) 116, 152
MmeI TCCRAC 3 cut(s) 171, 429, 455
Mox20I TGGCCA 1 cut(s) 707
MroXI GAANNNNTTC 2 cut(s) 98, 278
MscI TGGCCA 1 cut(s) 707
MseI TTAA 3 cut(s) 795, 837, 915
Msp20I TGGCCA 1 cut(s) 707
MspCI CTTAAG 1 cut(s) 914
MspI CCGG 6 cut(s) 69, 91, 117, 549, 723, 854
MspR9I CCNGG 7 cut(s) 70, 292, 613, 724, 855, 878, 1191
MvaI CCWGG 4 cut(s) 292, 613, 878, 1191
MvnI CGCG 4 cut(s) 37, 249, 317, 1146
MwoI GCNNNNNNNGC 3 cut(s) 371, 545, 719
NciI CCSGG 3 cut(s) 70, 724, 855
NdeII GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
NlaIII CATG 6 cut(s) 244, 680, 695, 751, 961, 1223
NlaIV GGNNCC 1 cut(s) 547
NmuCI GTSAC 5 cut(s) 226, 301, 770, 1264, 1276
PagI TCATGA 2 cut(s) 240, 691
PasI CCCWGGG 1 cut(s) 612
PdmI GAANNNNTTC 2 cut(s) 98, 278
PfeI GAWTC 1 cut(s) 330
Pfl23II CGTACG 1 cut(s) 324
PflMI CCANNNNNTGG 1 cut(s) 627
PkrI GCNGC 1 cut(s) 1251
PleI GAGTC 2 cut(s) 116, 151
PpsI GAGTC 2 cut(s) 116, 151
PpuMI RGGWCCY 1 cut(s) 1259
PsiI TTATAA 1 cut(s) 1209
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 1259
Psp6I CCWGG 4 cut(s) 290, 611, 876, 1189
PspGI CCWGG 4 cut(s) 290, 611, 876, 1189
PspLI CGTACG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 547
PspPI GGNCC 2 cut(s) 1187, 1259
PspPPI RGGWCCY 1 cut(s) 1259
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PsuI RGATCY 1 cut(s) 1075
RsaI GTAC 5 cut(s) 254, 287, 326, 731, 1052
RsaNI GTAC 5 cut(s) 253, 286, 325, 730, 1051
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 795, 837, 915
SatI GCNGC 1 cut(s) 1250
Sau3AI GATC 6 cut(s) 6, 43, 405, 669, 862, 1075
Sau96I GGNCC 2 cut(s) 1187, 1259
SchI GAGTC 2 cut(s) 116, 152
ScrFI CCNGG 7 cut(s) 70, 292, 613, 724, 855, 878, 1191
SduI GDGCHC 1 cut(s) 240
SexAI ACCWGGT 1 cut(s) 876
SfaNI GCATC 5 cut(s) 101, 552, 1012, 1033, 1298
SinI GGWCC 1 cut(s) 1259
SmlI CTYRAG 4 cut(s) 571, 914, 1105, 1340
SmoI CTYRAG 4 cut(s) 571, 914, 1105, 1340
Sse9I AATT 5 cut(s) 27, 591, 833, 941, 1149
SsiI CCGC 8 cut(s) 35, 169, 202, 247, 317, 786, 1095, 1146
SspI AATATT 1 cut(s) 686
SspMI CTAG 3 cut(s) 804, 1073, 1364
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 7 cut(s) 68, 290, 611, 722, 853, 876, 1189
StyI CCWWGG 2 cut(s) 159, 621
TaaI ACNGT 5 cut(s) 106, 307, 436, 637, 703
TaiI ACGT 3 cut(s) 303, 735, 764
TaqI TCGA 8 cut(s) 141, 153, 184, 282, 567, 668, 1233, 1335
TasI AATT 5 cut(s) 27, 591, 833, 941, 1149
TatI WGTACW 3 cut(s) 252, 285, 1050
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 3 cut(s) 795, 837, 915
Tru9I TTAA 3 cut(s) 795, 837, 915
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 5 cut(s) 226, 301, 770, 1264, 1276
TseI GCWGC 1 cut(s) 1249
Tsp45I GTSAC 5 cut(s) 226, 301, 770, 1264, 1276
TspDTI ATGAA 3 cut(s) 257, 473, 665
TspGWI ACGGA 3 cut(s) 342, 941, 987
TspRI CASTG 1 cut(s) 715
Van91I CCANNNNNTGG 1 cut(s) 627
Vha464I CTTAAG 1 cut(s) 914
VpaK11BI GGWCC 1 cut(s) 1259
XapI RAATTY 3 cut(s) 27, 833, 1149
XmnI GAANNNNTTC 2 cut(s) 98, 278
XspI CTAG 3 cut(s) 804, 1073, 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.