pycom05g13250

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0022425
Physical Location & Seq
Forward (+)
16834241 .. 16836221
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGAGGATTGGGATAAATTTGAAGACGAAGGATTCGCTTGTGTCAAGGAGCTCACTCTTGATGTGTCAAATGTCTTGGCACCTCCAAAACAGAAATCATCATCAGTTCAGAAAGAAAAAGCTCCCCCAGTTGAGAGTCCAACAACTGCTTATTCACCTAAAGTTGATGTTAAGTCAGAAAAGCCTCAAAGTACGGATGCCAAGGTTGTTGAAAATGGAGCAGCATATGATAAAAATGAGGACGAGTCAGCAAAAAGTGCTCCGAACAGTCCATTTGCCAGCAGTACTGTTGGAAGCCCATCTAGAGAATTTTCAGATTCTAACTATGGAAAGACGACAGACGCAGATGCCTCACCCTGTAATAAGGAATCTCAAAGGTATATTTCTAACTATGGTGACAAAGGTTCCGATGAACCAGCATGGGGGACATTTGACACTAATGATGACATTGACTCGGTGTGGGGTCTCAATGCAGTTAGTACCACCAAGGATATGGATCGGGAGATTAATAAGGATCACTACTTTTCTGGCCCTGGGGAGTTCTGGCCTCAACCCTATCAGGACTGGGTCATCACAAGGAGGCGGCTTTTCCCAGAAGAGCCGCCCTTTTACTTTTGACGATTCTCTTCCTAGCACACCATTCTCAGCCTTCGATTCAGGGTATTCACCGCCAAGGTACAAGGATAGCTCAGAACCTTCATTTGACACCTTCTCGAGGTTTGATTCGTTCAGAAGCAGACAAGATACTGGATTTTTCCCTCAACTAGAAACTCTCGGAAGATTTGATTCTATGCGCAGCAGTAGAGATTTTGATCGGGGTAATGGTTTTCCAACATTTGATGACATCCCAGACCCTTTCGGCTCTTCGGCGCCATTTGGGTCATTGCTGGACAGTCAAACTCCAAGAAGAGACTCAGACCCCTTTGGATCTTCAGGTCCATTTCGGACGTTACTGGACAGTCAAACTCCAAGAAGAGATTCGGACCCATTTGGGTCTTCAGGGCCTTTCCGGACGTCATTGGAGACTCCGAGAAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

347

Amino Acids

39.46

Weight (kDa)

10.86

Isoelectric Point (pI)

57.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022425)

Species Orthologous Gene IDs
malus_domestica MD05G1136400.v1.1 MD05G1136500.v1.1
pyrus_communis pycom05g13250
rosa_multiflora Rmu_sc0011374.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1016
Acc16I TGCGCA 1 cut(s) 794
AccB1I GGYRCC 2 cut(s) 78, 868
AccIII TCCGGA 1 cut(s) 1008
AciI CCGC 3 cut(s) 582, 601, 668
AclWI GGATC 3 cut(s) 503, 521, 934
AcsI RAATTY 2 cut(s) 16, 307
AcuI CTGAAG 2 cut(s) 915, 981
AcyI GRCGYC 2 cut(s) 869, 1013
AfaI GTAC 4 cut(s) 192, 285, 480, 677
AfiI CCNNNNNNNGG 2 cut(s) 421, 714
AgsI TTSAA 2 cut(s) 22, 211
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 961, 993
AluBI AGCT 3 cut(s) 51, 121, 687
AluI AGCT 3 cut(s) 51, 121, 687
Alw21I GWGCWC 2 cut(s) 53, 261
Alw26I GTCTC 3 cut(s) 469, 903, 1016
AlwI GGATC 3 cut(s) 503, 521, 934
Ama87I CYCGRG 1 cut(s) 712
Aor13HI TCCGGA 1 cut(s) 1008
AoxI GGCC 3 cut(s) 528, 544, 1001
ApeKI GCWGC 2 cut(s) 220, 795
ApoI RAATTY 2 cut(s) 16, 307
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AseI ATTAAT 1 cut(s) 506
Asp700I GAANNNNTTC 1 cut(s) 976
AspLEI GCGC 2 cut(s) 795, 871
AspS9I GGNCC 4 cut(s) 529, 935, 982, 1001
AsuHPI GGTGA 4 cut(s) 146, 344, 406, 657
AvaI CYCGRG 1 cut(s) 712
AvaII GGWCC 2 cut(s) 935, 982
BanI GGYRCC 2 cut(s) 78, 868
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 29, 987
Bbv12I GWGCWC 2 cut(s) 53, 261
BbvI GCAGC 2 cut(s) 232, 807
BccI CCATC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 533
BcoDI GTCTC 3 cut(s) 469, 903, 1016
BfaI CTAG 3 cut(s) 302, 630, 764
BfoI RGCGCY 1 cut(s) 872
BisI GCNGC 4 cut(s) 221, 583, 601, 796
BlsI GCNGC 4 cut(s) 222, 584, 602, 797
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 2 cut(s) 935, 982
BmeT110I CYCGRG 1 cut(s) 712
BmgT120I GGNCC 4 cut(s) 529, 935, 982, 1001
BmiI GGNNCC 4 cut(s) 80, 405, 870, 984
BmrFI CCNGG 1 cut(s) 533
BmrI ACTGGG 2 cut(s) 121, 573
BmsI GCATC 2 cut(s) 186, 336
BmuI ACTGGG 2 cut(s) 121, 573
BpiI GAAGAC 2 cut(s) 29, 987
BsaBI GATNNNNATC 2 cut(s) 494, 810
BsaHI GRCGYC 2 cut(s) 869, 1013
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 5 cut(s) 200, 485, 531, 532, 671
BsaWI WCCGGW 1 cut(s) 1008
Bsc4I CCNNNNNNNGG 2 cut(s) 421, 714
Bse1I ACTGG 4 cut(s) 127, 568, 751, 957
Bse3DI GCAATG 1 cut(s) 881
Bse8I GATNNNNATC 2 cut(s) 494, 810
BseAI TCCGGA 1 cut(s) 1008
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 5 cut(s) 200, 485, 531, 532, 671
BseGI GGATG 2 cut(s) 201, 843
BseJI GATNNNNATC 2 cut(s) 494, 810
BseLI CCNNNNNNNGG 2 cut(s) 421, 714
BseMI GCAATG 1 cut(s) 881
BseMII CTCAG 3 cut(s) 657, 702, 927
BseNI ACTGG 4 cut(s) 127, 568, 751, 957
BseXI GCAGC 2 cut(s) 232, 807
BshFI GGCC 3 cut(s) 530, 546, 1003
BshNI GGYRCC 2 cut(s) 78, 868
BsiHKAI GWGCWC 2 cut(s) 53, 261
BsiHKCI CYCGRG 1 cut(s) 712
BsiSI CCGG 1 cut(s) 1009
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 2 cut(s) 421, 714
BsmAI GTCTC 3 cut(s) 469, 903, 1016
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 3 cut(s) 530, 546, 1003
Bso31I GGTCTC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 712
Bsp1286I GDGCHC 2 cut(s) 53, 261
Bsp13I TCCGGA 1 cut(s) 1008
Bsp143I GATC 4 cut(s) 495, 513, 811, 926
BspACI CCGC 3 cut(s) 582, 601, 668
BspANI GGCC 3 cut(s) 530, 546, 1003
BspCNI CTCAG 3 cut(s) 656, 701, 926
BspEI TCCGGA 1 cut(s) 1008
BspLI GGNNCC 4 cut(s) 80, 405, 870, 984
BspPI GGATC 3 cut(s) 503, 521, 934
BspQI GCTCTTC 2 cut(s) 590, 868
BspT107I GGYRCC 2 cut(s) 78, 868
BspTNI GGTCTC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 881
BsrI ACTGG 4 cut(s) 127, 568, 751, 957
BssECI CCNNGG 5 cut(s) 200, 485, 531, 532, 671
BssMI GATC 4 cut(s) 495, 513, 811, 926
BssNI GRCGYC 2 cut(s) 869, 1013
BssT1I CCWWGG 3 cut(s) 200, 485, 671
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 4 cut(s) 268, 288, 893, 959
Bst6I CTCTTC 5 cut(s) 590, 630, 868, 901, 967
BstACI GRCGYC 2 cut(s) 869, 1013
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 3 cut(s) 643, 688, 913
BstENI CCTNNNNNAGG 1 cut(s) 712
BstF5I GGATG 2 cut(s) 201, 843
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 2 cut(s) 795, 871
BstKTI GATC 4 cut(s) 498, 516, 814, 929
BstMAI GTCTC 3 cut(s) 469, 903, 1016
BstMBI GATC 4 cut(s) 495, 513, 811, 926
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNI CCWGG 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 531
BstV1I GCAGC 2 cut(s) 232, 807
BstV2I GAAGAC 2 cut(s) 29, 987
BstX2I RGATCY 1 cut(s) 926
BstXI CCANNNNNNTGG 1 cut(s) 492
BstYI RGATCY 1 cut(s) 926
BsuRI GGCC 3 cut(s) 530, 546, 1003
BtsCI GGATG 2 cut(s) 201, 843
Cac8I GCNNGC 1 cut(s) 279
CfoI GCGC 2 cut(s) 795, 871
Cfr13I GGNCC 4 cut(s) 529, 935, 982, 1001
CseI GACGC 1 cut(s) 349
Csp6I GTAC 4 cut(s) 191, 284, 479, 676
CviAII CATG 1 cut(s) 419
CviQI GTAC 4 cut(s) 191, 284, 479, 676
DdeI CTNAG 3 cut(s) 643, 688, 913
DinI GGCGCC 1 cut(s) 870
DpnI GATC 4 cut(s) 497, 515, 813, 928
DpnII GATC 4 cut(s) 495, 513, 811, 926
Eam1104I CTCTTC 5 cut(s) 590, 630, 868, 901, 967
EarI CTCTTC 5 cut(s) 590, 630, 868, 901, 967
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 3 cut(s) 200, 485, 671
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 2 cut(s) 935, 982
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 915, 981
Eco88I CYCGRG 1 cut(s) 712
EcoICRI GAGCTC 1 cut(s) 51
EcoNI CCTNNNNNAGG 1 cut(s) 712
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 3 cut(s) 200, 485, 671
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 870
EheI GGCGCC 1 cut(s) 870
ErhI CCWWGG 3 cut(s) 200, 485, 671
FaeI CATG 1 cut(s) 422
FaiI YATR 8 cut(s) 225, 227, 326, 380, 392, 420, 493, 791
FaqI GGGAC 1 cut(s) 438
FatI CATG 1 cut(s) 418
FauNDI CATATG 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 221, 583, 601, 796
FokI GGATG 2 cut(s) 208, 830
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 4 cut(s) 221, 583, 601, 796
FspBI CTAG 3 cut(s) 302, 630, 764
FspI TGCGCA 1 cut(s) 794
GlaI GCGC 2 cut(s) 794, 870
GluI GCNGC 4 cut(s) 221, 583, 601, 796
HaeII RGCGCY 1 cut(s) 872
HaeIII GGCC 3 cut(s) 530, 546, 1003
HapII CCGG 1 cut(s) 1009
HgaI GACGC 1 cut(s) 349
HhaI GCGC 2 cut(s) 795, 871
Hin1I GRCGYC 2 cut(s) 869, 1013
Hin1II CATG 1 cut(s) 422
Hin6I GCGC 2 cut(s) 793, 869
HinP1I GCGC 2 cut(s) 793, 869
HpaII CCGG 1 cut(s) 1009
HphI GGTGA 4 cut(s) 146, 344, 406, 657
Hpy188III TCNNGA 6 cut(s) 58, 302, 499, 559, 712, 1009
HpyAV CCTTC 4 cut(s) 22, 658, 705, 718
HpyCH4III ACNGT 4 cut(s) 268, 288, 893, 959
HpyCH4IV ACGT 2 cut(s) 947, 1013
HpyCH4V TGCA 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 3 cut(s) 643, 688, 913
HpySE526I ACGT 2 cut(s) 947, 1013
Hsp92I GRCGYC 2 cut(s) 869, 1013
Hsp92II CATG 1 cut(s) 422
HspAI GCGC 2 cut(s) 793, 869
KasI GGCGCC 1 cut(s) 868
Kpn2I TCCGGA 1 cut(s) 1008
Kzo9I GATC 4 cut(s) 495, 513, 811, 926
LguI GCTCTTC 2 cut(s) 590, 868
LmnI GCTCC 4 cut(s) 48, 126, 217, 264
Lsp1109I GCAGC 2 cut(s) 232, 807
LweI GCATC 2 cut(s) 186, 336
MaeI CTAG 3 cut(s) 302, 630, 764
MaeII ACGT 2 cut(s) 947, 1013
MaeIII GTNAC 2 cut(s) 394, 948
MalI GATC 4 cut(s) 497, 515, 813, 928
MboI GATC 4 cut(s) 495, 513, 811, 926
MboII GAAGA 9 cut(s) 34, 607, 617, 789, 855, 918, 921, 984, 987
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 2 cut(s) 53, 261
MluCI AATT 2 cut(s) 16, 307
Mly113I GGCGCC 1 cut(s) 869
MlyI GAGTC 5 cut(s) 144, 253, 445, 905, 1018
MmeI TCCRAC 3 cut(s) 163, 270, 854
MnlI CCTC 8 cut(s) 92, 194, 231, 360, 557, 572, 708, 768
MroI TCCGGA 1 cut(s) 1008
MroXI GAANNNNTTC 1 cut(s) 976
MseI TTAA 2 cut(s) 170, 506
MspI CCGG 1 cut(s) 1009
MspR9I CCNGG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 1 cut(s) 256
NarI GGCGCC 1 cut(s) 869
NdeI CATATG 1 cut(s) 225
NdeII GATC 4 cut(s) 495, 513, 811, 926
NlaIII CATG 1 cut(s) 422
NlaIV GGNNCC 4 cut(s) 80, 405, 870, 984
NmuCI GTSAC 1 cut(s) 394
NsbI TGCGCA 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 712
PasI CCCWGGG 1 cut(s) 532
PciSI GCTCTTC 2 cut(s) 590, 868
PdmI GAANNNNTTC 1 cut(s) 976
PfeI GAWTC 8 cut(s) 32, 316, 367, 620, 653, 722, 785, 977
PflFI GACNNNGTC 1 cut(s) 565
PkrI GCNGC 4 cut(s) 222, 584, 602, 797
PleI GAGTC 5 cut(s) 143, 252, 445, 905, 1018
PluTI GGCGCC 1 cut(s) 872
PpsI GAGTC 5 cut(s) 143, 252, 445, 905, 1018
PshBI ATTAAT 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 4 cut(s) 80, 405, 870, 984
PspPI GGNCC 4 cut(s) 529, 935, 982, 1001
PsuI RGATCY 1 cut(s) 926
PsyI GACNNNGTC 1 cut(s) 565
RsaI GTAC 4 cut(s) 192, 285, 480, 677
RsaNI GTAC 4 cut(s) 191, 284, 479, 676
SacI GAGCTC 1 cut(s) 53
SapI GCTCTTC 2 cut(s) 590, 868
SaqAI TTAA 2 cut(s) 170, 506
SatI GCNGC 4 cut(s) 221, 583, 601, 796
Sau3AI GATC 4 cut(s) 495, 513, 811, 926
Sau96I GGNCC 4 cut(s) 529, 935, 982, 1001
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 5 cut(s) 144, 253, 445, 905, 1018
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 2 cut(s) 53, 261
SfaNI GCATC 2 cut(s) 186, 336
SfoI GGCGCC 1 cut(s) 870
Sfr274I CTCGAG 1 cut(s) 712
SinI GGWCC 2 cut(s) 935, 982
SlaI CTCGAG 1 cut(s) 712
SmlI CTYRAG 1 cut(s) 712
SmoI CTYRAG 1 cut(s) 712
Sse9I AATT 2 cut(s) 16, 307
SsiI CCGC 3 cut(s) 582, 601, 668
SspDI GGCGCC 1 cut(s) 868
SspMI CTAG 3 cut(s) 302, 630, 764
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 3 cut(s) 200, 485, 671
TaaI ACNGT 4 cut(s) 268, 288, 893, 959
TaiI ACGT 2 cut(s) 950, 1016
TaqI TCGA 2 cut(s) 651, 713
TasI AATT 2 cut(s) 16, 307
TatI WGTACW 1 cut(s) 283
TauI GCSGC 2 cut(s) 585, 603
TfiI GAWTC 8 cut(s) 32, 316, 367, 620, 653, 722, 785, 977
Tru1I TTAA 2 cut(s) 170, 506
Tru9I TTAA 2 cut(s) 170, 506
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 2 cut(s) 220, 795
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 2 cut(s) 425, 687
TspGWI ACGGA 1 cut(s) 208
Tth111I GACNNNGTC 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 935, 982
VspI ATTAAT 1 cut(s) 506
XagI CCTNNNNNAGG 1 cut(s) 712
XapI RAATTY 2 cut(s) 16, 307
XbaI TCTAGA 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 489
XhoI CTCGAG 1 cut(s) 712
XmnI GAANNNNTTC 1 cut(s) 976
XspI CTAG 3 cut(s) 302, 630, 764
ZraI GACGTC 1 cut(s) 1014
ZrmI AGTACT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.