pycom05g17420
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0020214
Physical Location & Seq
Forward (+)
20578421 .. 20578861
441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17420.1

Sequence Viewer

Length: 441 bp
ATGTTGTTGATGATGCCTGCTAGTGGGAATTGTTACTTGAAAGCTTCATCTGTGGCTTTACCTGTTTCTAGTTACACCATCATTCTTGCTGCTTCCTTTTGCTTCCAGCCATCTCTCTATAAAATGGCACACGGTCCAGGTGGAGGAGGAACTAAAAACATGAAAGTTTCTAGCATCTCTGTCTGTTGGAAAATCCAGTGTTCGAAGCCATTGAAGGGAATCACAAGCGCTGGGTCTGAGGTCAAGCTTGTTGGCAATCAAAGCAGTGCATCCTCAATGGGTTATTGCCGCGGTTCATATCTTTTGAGGCAGTTGTTTTGGAAGCTGAAGTCACAATGGAAACAAGGTTTGGGATGGCAAAAGAGCTACAACCGGTACAGCTATGATCTTCAGAGCTACTCTCTCAACTTCGACAACGGGTTTGATGACCATCGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

147

Amino Acids

16.11

Weight (kDa)

9.6

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44005
prunus_persica Prupe.8G216600_v2.0.a1
pyrus_communis pycom05g17420
rosa_chinensis RchiOBHm_Chr6g0286891
rosa_rugosa Rorug06G0184600
rosa_samantha Rh6BG301100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 291
AciI CCGC 2 cut(s) 289, 291
AcuI CTGAAG 2 cut(s) 347, 374
AfaI GTAC 1 cut(s) 377
AfeI AGCGCT 1 cut(s) 229
AfiI CCNNNNNNNGG 3 cut(s) 23, 143, 215
AgeI ACCGGT 1 cut(s) 372
AgsI TTSAA 2 cut(s) 40, 214
AjnI CCWGG 1 cut(s) 136
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 6 cut(s) 44, 247, 325, 366, 381, 396
AluI AGCT 6 cut(s) 44, 247, 325, 366, 381, 396
Aor51HI AGCGCT 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AsiGI ACCGGT 1 cut(s) 372
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 203
AvaII GGWCC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 76
BccI CCATC 4 cut(s) 86, 118, 348, 438
BciT130I CCWGG 1 cut(s) 138
BfaI CTAG 3 cut(s) 21, 69, 171
BfoI RGCGCY 1 cut(s) 231
BisI GCNGC 2 cut(s) 90, 289
BlsI GCNGC 2 cut(s) 91, 290
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 3 cut(s) 3, 183, 278
BplI GAGNNNNNCTC 2 cut(s) 385, 417
Bpu14I TTCGAA 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 429
BsaJI CCNNGG 1 cut(s) 289
BsaWI WCCGGW 1 cut(s) 372
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 143, 215
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 196
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 2 cut(s) 269, 359
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 23, 143, 215
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 291
BshTI ACCGGT 1 cut(s) 372
BsiSI CCGG 1 cut(s) 373
BslI CCNNNNNNNGG 3 cut(s) 23, 143, 215
Bsp119I TTCGAA 1 cut(s) 203
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 2 cut(s) 289, 291
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 1 cut(s) 291
BspT104I TTCGAA 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 237
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 269, 359
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 416
BtsCI GGATG 2 cut(s) 269, 359
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 203, 271
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 1 cut(s) 230
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 134
Cfr42I CCGCGG 1 cut(s) 292
Csp6I GTAC 1 cut(s) 376
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 1 cut(s) 160
CviJI RGCY 9 cut(s) 44, 56, 109, 208, 247, 325, 366, 381, 396
CviKI_1 RGCY 9 cut(s) 44, 56, 109, 208, 247, 325, 366, 381, 396
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 1 cut(s) 237
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 347, 374
EcoRII CCWGG 1 cut(s) 136
FaeI CATG 1 cut(s) 163
FaiI YATR 5 cut(s) 120, 161, 298, 384, 439
FatI CATG 1 cut(s) 159
Fnu4HI GCNGC 2 cut(s) 90, 289
FokI GGATG 2 cut(s) 256, 366
Fsp4HI GCNGC 2 cut(s) 90, 289
FspBI CTAG 3 cut(s) 21, 69, 171
GlaI GCGC 1 cut(s) 229
GluI GCNGC 2 cut(s) 90, 289
GsaI CCCAGC 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 231
HapII CCGG 1 cut(s) 373
HhaI GCGC 1 cut(s) 230
Hin1II CATG 1 cut(s) 163
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HindIII AAGCTT 2 cut(s) 42, 245
HinfI GANTC 1 cut(s) 219
HpaII CCGG 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 238, 393
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 1 cut(s) 134
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 1 cut(s) 237
Hsp92II CATG 1 cut(s) 163
HspAI GCGC 1 cut(s) 228
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 1 cut(s) 385
LpnPI CCDG 8 cut(s) 30, 75, 119, 123, 150, 209, 216, 386
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 3 cut(s) 3, 183, 278
MaeI CTAG 3 cut(s) 21, 69, 171
MaeIII GTNAC 3 cut(s) 32, 71, 330
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 1 cut(s) 380
MluCI AATT 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 5 cut(s) 137, 140, 232, 283, 300
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 1 cut(s) 385
NlaIII CATG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 330
NspV TTCGAA 1 cut(s) 203
PfeI GAWTC 1 cut(s) 219
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 2 cut(s) 91, 290
Psp6I CCWGG 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 136
PspPI GGNCC 1 cut(s) 134
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SacII CCGCGG 1 cut(s) 292
SatI GCNGC 2 cut(s) 90, 289
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 138
SfaNI GCATC 3 cut(s) 3, 183, 278
Sfr303I CCGCGG 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 203
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 134
Sse9I AATT 1 cut(s) 28
SsiI CCGC 2 cut(s) 289, 291
SspMI CTAG 3 cut(s) 21, 69, 171
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 134
TaqI TCGA 2 cut(s) 203, 411
TasI AATT 1 cut(s) 28
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 1 cut(s) 219
TscAI CASTG 2 cut(s) 203, 271
TseFI GTSAC 1 cut(s) 330
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 1 cut(s) 330
TspDTI ATGAA 3 cut(s) 36, 176, 285
TspRI CASTG 2 cut(s) 203, 271
VpaK11BI GGWCC 1 cut(s) 134
XspI CTAG 3 cut(s) 21, 69, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.