pycom05g22020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018329
Physical Location & Seq
Forward (+)
24562455 .. 24562832
378 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22020.1

Sequence Viewer

Length: 378 bp
ATGGCTAGTAATTATGCAAAATCACCTACCACCACTTTTACAACCCTCCTCTTCTTTCTTCTCATCACATTTCAAATCACCATGGCTAAAAAGAAGCATAGCTTCCCAATCCCACCTGTCAGTAGTACTTCCTCGAGATCACATGGAGTTGGAGTCATCAGTAAGAAAAATGTTATCGATGAATATGCTACAAGAAGATCCTCACATGGCATCATCGATTCAAAGGCTGATCACGAGTTTACAGGCGAATCGGGACGAAGGAGAGGACTAGGGTTTCGGGTTCGTCGTGGGAGGCAGCCATTCCCATGGAAAGAAAATATGTTCAGAGCCGGTGCACATGAAGTTCCAAGTGGTCCGAATCCCATTTCAAATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.98

Weight (kDa)

11.19

Isoelectric Point (pI)

61.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34925
fragaria_vesca FvH4_3g13250
pyrus_communis pycom05g22020 pycom10g18950
rosa_chinensis RchiOBHm_Chr5g0021721
rosa_roxburghii Rroxscaffold_1G00057000
rosa_samantha Rh5AG157200 Rh5BG156900 Rh5CG171000 Rh5DG157000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 192
AfaI GTAC 1 cut(s) 127
AgsI TTSAA 3 cut(s) 74, 222, 369
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 337
Alw44I GTGCAC 1 cut(s) 333
AlwI GGATC 1 cut(s) 192
Ama87I CYCGRG 1 cut(s) 133
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 1 cut(s) 295
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 2 cut(s) 15, 70
AvaI CYCGRG 1 cut(s) 133
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 337
BauI CACGAG 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BclI TGATCA 1 cut(s) 229
BfaI CTAG 2 cut(s) 6, 269
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
BmcAI AGTACT 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 353
BmsI GCATC 1 cut(s) 219
Bsa29I ATCGAT 2 cut(s) 177, 216
BsaJI CCNNGG 2 cut(s) 81, 305
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 329
BseCI ATCGAT 2 cut(s) 177, 216
BseDI CCNNGG 2 cut(s) 81, 305
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 1 cut(s) 307
BshVI ATCGAT 2 cut(s) 177, 216
BsiHKAI GWGCWC 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 3 cut(s) 137, 197, 229
Bsp19I CCATGG 2 cut(s) 81, 305
BspDI ATCGAT 2 cut(s) 177, 216
BspPI GGATC 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 81, 305
BssMI GATC 3 cut(s) 137, 197, 229
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 81, 305
Bst2BI CACGAG 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 56
BstDSI CCRYGG 2 cut(s) 81, 305
BstKTI GATC 3 cut(s) 140, 200, 232
BstMBI GATC 3 cut(s) 137, 197, 229
BstSLI GKGCMC 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 307
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 1 cut(s) 197
Bsu15I ATCGAT 2 cut(s) 177, 216
BsuTUI ATCGAT 2 cut(s) 177, 216
BtgI CCRYGG 2 cut(s) 81, 305
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 1 cut(s) 353
ClaI ATCGAT 2 cut(s) 177, 216
Csp6I GTAC 1 cut(s) 126
CviAII CATG 5 cut(s) 82, 143, 206, 306, 338
CviJI RGCY 6 cut(s) 5, 86, 102, 227, 298, 329
CviKI_1 RGCY 6 cut(s) 5, 86, 102, 227, 298, 329
CviQI GTAC 1 cut(s) 126
DpnI GATC 3 cut(s) 139, 199, 231
DpnII GATC 3 cut(s) 137, 197, 229
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 81, 305
Eco47I GGWCC 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 133
EcoT14I CCWWGG 2 cut(s) 81, 305
ErhI CCWWGG 2 cut(s) 81, 305
FaeI CATG 5 cut(s) 85, 146, 209, 309, 341
FaiI YATR 9 cut(s) 15, 83, 99, 144, 186, 207, 307, 320, 339
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 1 cut(s) 267
FatI CATG 5 cut(s) 81, 142, 205, 305, 337
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 2 cut(s) 6, 269
GluI GCNGC 1 cut(s) 296
HapII CCGG 1 cut(s) 330
Hin1II CATG 5 cut(s) 85, 146, 209, 309, 341
HinfI GANTC 4 cut(s) 153, 218, 248, 358
HpaII CCGG 1 cut(s) 330
HphI GGTGA 2 cut(s) 15, 70
Hpy166II GTNNAC 2 cut(s) 240, 335
Hpy188I TCNGA 2 cut(s) 326, 357
Hpy188III TCNNGA 3 cut(s) 135, 233, 252
Hpy8I GTNNAC 2 cut(s) 240, 335
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 17, 335
Hsp92II CATG 5 cut(s) 85, 146, 209, 309, 341
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 3 cut(s) 137, 197, 229
LpnPI CCDG 3 cut(s) 129, 228, 343
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 1 cut(s) 219
MaeI CTAG 2 cut(s) 6, 269
MalI GATC 3 cut(s) 139, 199, 231
MboI GATC 3 cut(s) 137, 197, 229
MboII GAAGA 3 cut(s) 43, 50, 207
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 1 cut(s) 10
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 6 cut(s) 56, 59, 142, 211, 257, 285
MslI CAYNNNNRTG 1 cut(s) 304
MspI CCGG 1 cut(s) 330
NcoI CCATGG 2 cut(s) 81, 305
NdeII GATC 3 cut(s) 137, 197, 229
NlaIII CATG 5 cut(s) 85, 146, 209, 309, 341
PaeR7I CTCGAG 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PfeI GAWTC 3 cut(s) 218, 248, 358
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PspPI GGNCC 1 cut(s) 353
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 1 cut(s) 304
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 3 cut(s) 137, 197, 229
Sau96I GGNCC 1 cut(s) 353
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 1 cut(s) 162
SduI GDGCHC 1 cut(s) 337
SetI ASST 3 cut(s) 28, 104, 118
SfaNI GCATC 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 133
SinI GGWCC 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
Sse9I AATT 1 cut(s) 10
SspMI CTAG 2 cut(s) 6, 269
StyI CCWWGG 2 cut(s) 81, 305
TaqI TCGA 3 cut(s) 134, 177, 216
TasI AATT 1 cut(s) 10
TatI WGTACW 1 cut(s) 125
TfiI GAWTC 3 cut(s) 218, 248, 358
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 195, 354
VneI GTGCAC 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 133
XspI CTAG 2 cut(s) 6, 269
ZrmI AGTACT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.