pycom05g23100

acyltransferase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016182
Physical Location & Seq
Forward (+)
25128779 .. 25132727
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1575 bp
ATGGCGATGCTTTTGGAGGATATTCTGAAGTCGGTGGAGCTCTGGTTAAAGCTGATCAAGAAGCCCCAGCCTTACGTGGACCCAAATCTCGACCCGGTTCTGCTAGTTCCGGGCATCGCCGGTTCGGTCTTGAACGCCGTCGATGAAGAGAGTGGTGCGGAGGAGCGCGTTTGGATTCGAATCCTCGGTGCTGACTACACGTTCCGGACCAAGCTTTGGTCTCGTTTCGATCCTTCAACAGGTAAAACTGAGTCTATGGATCCAAACACAAGGATAGTGGTTCCTGAAGGTAGATGTGGACTTGAGGCAATCGACGCCTTGGACCCTGACATGGTCATTGGACAGGAGTGTGTATACTATTTCCATGACATGATTGTTGAATTGATCAAGTGGGGTTTCCAAGAGGGAACGACACTTTTTGGGTTTGGTTATGATTTCCGCCAAAGCAACAGGTTGCAGGAGACATTGGATCGCTTAGCTGCAAAACTAGAGGCAGTGTATAATGCTTCTGGAGGGAAAAAGATAACCATCATAACTCATTCTATGGGGGGCCTTCTAGTGAAATGTTTCATGTGCTTGCATAGTGATGTTTTTGAGAAATATGTGAACAAGTGGATTGCAATTGCTGCACCATTCCAGGGTGCGCCTGGATACATCACATCTACTTTTTTAAATGGAATGTCATTTGTTGACGGGTGGGAACAGAATTTTTTTATATCAAAATGGAGCATGCACCAACTGCTTATGGAGTGCCCATCAGTATATGAATTGATGGCTTGTCTTGATTTTCAATGGGAACATCCTCCGCTTCTGGAAATGTGGAGAGGTAGGCCAGATGGTGATGAGAAATCTCAAATTCTGCTAGAATCTTACCCCCTGGTAGAGAGCGTACAGATTTTTACAGAAGCTCTTGCAAATAATACGGTCAATTATAACGGTGAAGATATTCCCCTACCATTTAATATGGAAATCTTGAAATGGGCTAATGAAACACGCAACATTATATCTCGGGCGAAACTTCCTCCTCAAGTTAAATTCTACAATATATATGGGATGAATCTTGAGACACCTCATAGTGTTTGCTATGGAAACGAGGAAACACCGGTCACAGATCTACAACAGCTACGCTATTTCCAGCCTAAGTATGTATGCGTCGATGGTGATGGGACAGTTCCAGTAGAATCAGCTAAGGCAGATGGTCTCGATGCTGTAGCAAGGGTTGGAGTCCCTGGCGAGCACCGAGGAATTCTCTGCGAGCACCATGTGTTCCGGATACTCAAGCACTGGTTGAAGGCGGACCATGACCCGTACTACAACCCCGTAAACGACTACGTGATTCTACCCACTGCATTGGAAATGGAGAGGCACAAAGAGAGAGGTCGAGAAGTAACATCTGTAAAAGAGGAGTGGGAAATCATCTCCGAATGCCAAGACGCCGATGCAAAGCCTGTGGTGAGTTCCATATCGGTTTCGGACGCCCGAGCCGAGGCCCGCGCAACTCTCACTGTTCACCCTCGGAACGAGGGAAAGCAACATGTGGAGCTGAATGCTCGAAGCGTCTCAGTCGATGCATGA

Protein Analysis

525

Amino Acids

59.39

Weight (kDa)

5.02

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 76 - 397 2.8e-27 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19860
fragaria_vesca FvH4_3g12200
malus_domestica MD05G1252800.v1.1 MD10G1233900.v1.1
prunus_persica Prupe.4G111100_v2.0.a1
pyrus_communis pycom05g23100 pycom10g19620
rosa_chinensis RchiOBHm_Chr5g0020001
rosa_laevigata RLG00000032492
rosa_multiflora Rmu_sc0002250.1_g000005
rosa_roxburghii Rroxscaffold_1G00058190
rosa_rugosa Rorug05G0056100
rosa_samantha Rh5AG146300 Rh5BG145800
rosa_wichuraiana Rw5G012920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
AccB7I CCANNNNNTGG 1 cut(s) 216
AccI GTMKAC 1 cut(s) 354
AccII CGCG 2 cut(s) 168, 1494
AccIII TCCGGA 2 cut(s) 204, 1269
AciI CCGC 5 cut(s) 158, 439, 806, 1295, 1492
AclWI GGATC 4 cut(s) 224, 254, 267, 477
AcsI RAATTY 4 cut(s) 706, 855, 1034, 1245
AcuI CTGAAG 2 cut(s) 47, 306
AcyI GRCGYC 3 cut(s) 315, 1434, 1476
AdeI CACNNNGTG 1 cut(s) 1264
AfaI GTAC 2 cut(s) 891, 1310
AfiI CCNNNNNNNGG 5 cut(s) 216, 239, 331, 638, 1486
AflIII ACRYGT 2 cut(s) 198, 1534
AgeI ACCGGT 1 cut(s) 1102
AgsI TTSAA 6 cut(s) 133, 237, 380, 791, 976, 1291
AjnI CCWGG 4 cut(s) 636, 646, 876, 1228
AluBI AGCT 8 cut(s) 40, 52, 214, 479, 908, 1123, 1187, 1543
AluI AGCT 8 cut(s) 40, 52, 214, 479, 908, 1123, 1187, 1543
Alw21I GWGCWC 3 cut(s) 42, 1239, 1260
Alw26I GTCTC 5 cut(s) 225, 455, 1058, 1205, 1564
AlwI GGATC 4 cut(s) 224, 254, 267, 477
Ama87I CYCGRG 2 cut(s) 1008, 1479
Aor13HI TCCGGA 2 cut(s) 204, 1269
AoxI GGCC 3 cut(s) 550, 830, 1488
ApeKI GCWGC 2 cut(s) 479, 626
ApoI RAATTY 4 cut(s) 706, 855, 1034, 1245
AsiGI ACCGGT 1 cut(s) 1102
Asp700I GAANNNNTTC 2 cut(s) 566, 945
AspLEI GCGC 3 cut(s) 168, 646, 1496
AspS9I GGNCC 6 cut(s) 79, 207, 322, 550, 1297, 1489
AsuC2I CCSGG 2 cut(s) 95, 111
AsuHPI GGTGA 5 cut(s) 851, 950, 1172, 1465, 1502
AsuII TTCGAA 1 cut(s) 178
AvaI CYCGRG 2 cut(s) 1008, 1479
AvaII GGWCC 4 cut(s) 79, 207, 322, 1297
BaeGI GKGCMC 1 cut(s) 755
BamHI GGATCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 3 cut(s) 42, 1239, 1260
BbvI GCAGC 2 cut(s) 466, 613
BccI CCATC 7 cut(s) 536, 763, 766, 830, 1151, 1157, 1190
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 4 cut(s) 638, 648, 878, 1230
BciVI GTATCC 2 cut(s) 644, 1266
BclI TGATCA 2 cut(s) 54, 384
BcnI CCSGG 2 cut(s) 95, 111
BcoDI GTCTC 5 cut(s) 225, 455, 1058, 1205, 1564
BfaI CTAG 4 cut(s) 104, 488, 557, 863
BfmI CTRYAG 1 cut(s) 1209
BfuI GTATCC 2 cut(s) 644, 1266
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 2 cut(s) 480, 627
BlpI GCTNAGC 1 cut(s) 475
BlsI GCNGC 2 cut(s) 481, 628
Bme1390I CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
Bme18I GGWCC 4 cut(s) 79, 207, 322, 1297
BmeT110I CYCGRG 2 cut(s) 1008, 1479
BmgT120I GGNCC 6 cut(s) 79, 207, 322, 550, 1297, 1489
BmiI GGNNCC 5 cut(s) 81, 261, 282, 324, 551
BmrFI CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
BmsI GCATC 4 cut(s) 123, 1195, 1429, 1558
BplI GAGNNNNNCTC 2 cut(s) 1233, 1265
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 1188
Bpu1102I GCTNAGC 1 cut(s) 475
Bpu14I TTCGAA 1 cut(s) 178
BpuEI CTTGAG 4 cut(s) 323, 1011, 1082, 1262
BpuMI CCSGG 2 cut(s) 95, 111
BsaAI YACGTR 2 cut(s) 76, 1333
BsaBI GATNNNNATC 2 cut(s) 179, 527
BsaHI GRCGYC 3 cut(s) 315, 1434, 1476
BsaI GGTCTC 2 cut(s) 225, 1205
BsaJI CCNNGG 8 cut(s) 184, 318, 637, 876, 1228, 1240, 1485, 1514
BsaWI WCCGGW 3 cut(s) 204, 1102, 1269
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 239, 331, 638, 1486
Bse118I RCCGGY 2 cut(s) 119, 1102
Bse1I ACTGG 2 cut(s) 1175, 1289
Bse8I GATNNNNATC 2 cut(s) 179, 527
BseAI TCCGGA 2 cut(s) 204, 1269
BseBI CCWGG 4 cut(s) 638, 648, 878, 1230
BseDI CCNNGG 8 cut(s) 184, 318, 637, 876, 1228, 1240, 1485, 1514
BseGI GGATG 2 cut(s) 799, 1059
BseJI GATNNNNATC 2 cut(s) 179, 527
BseLI CCNNNNNNNGG 5 cut(s) 216, 239, 331, 638, 1486
BseMII CTCAG 2 cut(s) 240, 1575
BseNI ACTGG 2 cut(s) 1175, 1289
BseRI GAGGAG 3 cut(s) 176, 1014, 1418
BseSI GKGCMC 1 cut(s) 755
BseXI GCAGC 2 cut(s) 466, 613
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 2 cut(s) 168, 1494
BshFI GGCC 3 cut(s) 552, 832, 1490
BshTI ACCGGT 1 cut(s) 1102
BsiHKAI GWGCWC 3 cut(s) 42, 1239, 1260
BsiHKCI CYCGRG 2 cut(s) 1008, 1479
BsiSI CCGG 6 cut(s) 95, 110, 120, 205, 1103, 1270
BslFI GGGAC 2 cut(s) 1180, 1211
BslI CCNNNNNNNGG 5 cut(s) 216, 239, 331, 638, 1486
BsmAI GTCTC 5 cut(s) 225, 455, 1058, 1205, 1564
BsmBI CGTCTC 1 cut(s) 1564
BsmFI GGGAC 2 cut(s) 1180, 1211
BsmI GAATGC 2 cut(s) 1430, 1552
BsnI GGCC 3 cut(s) 552, 832, 1490
Bso31I GGTCTC 2 cut(s) 225, 1205
BsoBI CYCGRG 2 cut(s) 1008, 1479
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 4 cut(s) 42, 755, 1239, 1260
Bsp13I TCCGGA 2 cut(s) 204, 1269
Bsp143I GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
Bsp1720I GCTNAGC 1 cut(s) 475
BspACI CCGC 5 cut(s) 158, 439, 806, 1295, 1492
BspANI GGCC 3 cut(s) 552, 832, 1490
BspCNI CTCAG 2 cut(s) 241, 1574
BspEI TCCGGA 2 cut(s) 204, 1269
BspFNI CGCG 2 cut(s) 168, 1494
BspLI GGNNCC 5 cut(s) 81, 261, 282, 324, 551
BspPI GGATC 4 cut(s) 224, 254, 267, 477
BspT104I TTCGAA 1 cut(s) 178
BspTNI GGTCTC 2 cut(s) 225, 1205
BsrFI RCCGGY 2 cut(s) 119, 1102
BsrI ACTGG 2 cut(s) 1175, 1289
BssAI RCCGGY 2 cut(s) 119, 1102
BssECI CCNNGG 8 cut(s) 184, 318, 637, 876, 1228, 1240, 1485, 1514
BssMI GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
BssNAI GTATAC 1 cut(s) 355
BssNI GRCGYC 3 cut(s) 315, 1434, 1476
BssT1I CCWWGG 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 355
Bst2UI CCWGG 4 cut(s) 638, 648, 878, 1230
Bst4CI ACNGT 4 cut(s) 925, 938, 1171, 1507
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 3 cut(s) 315, 1434, 1476
BstAPI GCANNNNNTGC 2 cut(s) 626, 739
BstBAI YACGTR 2 cut(s) 76, 1333
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 5 cut(s) 578, 731, 1235, 1256, 1492
BstDEI CTNAG 5 cut(s) 249, 475, 1140, 1188, 1561
BstENI CCTNNNNNAGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 799, 1059
BstFNI CGCG 2 cut(s) 168, 1494
BstHHI GCGC 3 cut(s) 168, 646, 1496
BstKTI GATC 6 cut(s) 57, 232, 262, 387, 472, 1114
BstMAI GTCTC 5 cut(s) 225, 455, 1058, 1205, 1564
BstMBI GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 314, 626, 739
BstNI CCWGG 4 cut(s) 638, 648, 878, 1230
BstNSI RCATGY 2 cut(s) 733, 1538
BstSCI CCNGG 6 cut(s) 93, 109, 636, 646, 876, 1228
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 1 cut(s) 755
BstUI CGCG 2 cut(s) 168, 1494
BstV1I GCAGC 2 cut(s) 466, 613
BstX2I RGATCY 2 cut(s) 259, 1111
BstXI CCANNNNNNTGG 1 cut(s) 1351
BstYI RGATCY 2 cut(s) 259, 1111
BstZ17I GTATAC 1 cut(s) 355
BsuI GTATCC 2 cut(s) 644, 1266
BsuRI GGCC 3 cut(s) 552, 832, 1490
BtgZI GCGATG 2 cut(s) 20, 100
BtsCI GGATG 2 cut(s) 799, 1059
BtsI GCAGTG 2 cut(s) 501, 1344
BtsIMutI CAGTG 4 cut(s) 501, 1282, 1344, 1503
Cac8I GCNNGC 5 cut(s) 578, 731, 1235, 1256, 1492
CfoI GCGC 3 cut(s) 168, 646, 1496
Cfr10I RCCGGY 2 cut(s) 119, 1102
Cfr13I GGNCC 6 cut(s) 79, 207, 322, 550, 1297, 1489
CseI GACGC 5 cut(s) 323, 1141, 1442, 1484, 1546
Csp6I GTAC 2 cut(s) 890, 1309
CspAI ACCGGT 1 cut(s) 1102
CspCI CAANNNNNGTGG 4 cut(s) 258, 293, 1431, 1466
CviAII CATG 9 cut(s) 331, 365, 370, 571, 730, 1262, 1301, 1535, 1572
CviQI GTAC 2 cut(s) 890, 1309
DdeI CTNAG 5 cut(s) 249, 475, 1140, 1188, 1561
DpnI GATC 6 cut(s) 56, 231, 261, 386, 471, 1113
DpnII GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
DraI TTTAAA 1 cut(s) 672
DraIII CACNNNGTG 1 cut(s) 1264
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 2 cut(s) 428, 1310
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 2 cut(s) 225, 1205
Eco47I GGWCC 4 cut(s) 79, 207, 322, 1297
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 47, 306
Eco88I CYCGRG 2 cut(s) 1008, 1479
EcoICRI GAGCTC 1 cut(s) 40
EcoNI CCTNNNNNAGG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 550
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 4 cut(s) 636, 646, 876, 1228
EcoT14I CCWWGG 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 1573
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 1564
FaeI CATG 9 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1538, 1575
FaqI GGGAC 2 cut(s) 1180, 1211
FatI CATG 9 cut(s) 330, 364, 369, 570, 729, 1261, 1300, 1534, 1571
FauI CCCGC 1 cut(s) 1499
FbaI TGATCA 2 cut(s) 54, 384
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 480, 627
FokI GGATG 2 cut(s) 786, 1066
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 2 cut(s) 480, 627
FspBI CTAG 4 cut(s) 104, 488, 557, 863
GlaI GCGC 3 cut(s) 167, 645, 1495
GluI GCNGC 2 cut(s) 480, 627
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 3 cut(s) 552, 832, 1490
HapII CCGG 6 cut(s) 95, 110, 120, 205, 1103, 1270
HgaI GACGC 5 cut(s) 323, 1141, 1442, 1484, 1546
HhaI GCGC 3 cut(s) 168, 646, 1496
Hin1I GRCGYC 3 cut(s) 315, 1434, 1476
Hin1II CATG 9 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1538, 1575
Hin6I GCGC 3 cut(s) 166, 644, 1494
HinP1I GCGC 3 cut(s) 166, 644, 1494
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 8 cut(s) 175, 180, 251, 866, 1057, 1181, 1224, 1336
HpaII CCGG 6 cut(s) 95, 110, 120, 205, 1103, 1270
HphI GGTGA 5 cut(s) 851, 950, 1172, 1465, 1502
Hpy166II GTNNAC 7 cut(s) 79, 299, 355, 607, 691, 1324, 1510
Hpy188I TCNGA 4 cut(s) 27, 1423, 1474, 1518
Hpy8I GTNNAC 7 cut(s) 79, 299, 355, 607, 691, 1324, 1510
Hpy99I CGWCG 3 cut(s) 143, 317, 1157
HpyAV CCTTC 4 cut(s) 243, 281, 563, 1285
HpyCH4III ACNGT 4 cut(s) 925, 938, 1171, 1507
HpyCH4IV ACGT 3 cut(s) 75, 200, 1332
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 626, 739
HpyF3I CTNAG 5 cut(s) 249, 475, 1140, 1188, 1561
HpySE526I ACGT 3 cut(s) 75, 200, 1332
Hsp92I GRCGYC 3 cut(s) 315, 1434, 1476
Hsp92II CATG 9 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1538, 1575
HspAI GCGC 3 cut(s) 166, 644, 1494
Kpn2I TCCGGA 2 cut(s) 204, 1269
Ksp22I TGATCA 2 cut(s) 54, 384
Kzo9I GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
LmnI GCTCC 4 cut(s) 37, 163, 726, 1540
Lsp1109I GCAGC 2 cut(s) 466, 613
LweI GCATC 4 cut(s) 123, 1195, 1429, 1558
MaeI CTAG 4 cut(s) 104, 488, 557, 863
MaeII ACGT 3 cut(s) 75, 200, 1332
MaeIII GTNAC 2 cut(s) 1105, 1387
MalI GATC 6 cut(s) 56, 231, 261, 386, 471, 1113
MboI GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
MboII GAAGA 2 cut(s) 158, 953
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 2 cut(s) 259, 1111
MhlI GDGCHC 4 cut(s) 42, 755, 1239, 1260
MluCI AATT 8 cut(s) 380, 621, 706, 767, 855, 928, 1034, 1245
MlyI GAGTC 2 cut(s) 260, 1233
MmeI TCCRAC 1 cut(s) 1201
Mph1103I ATGCAT 1 cut(s) 1573
MroI TCCGGA 2 cut(s) 204, 1269
MroXI GAANNNNTTC 2 cut(s) 566, 945
MseI TTAA 4 cut(s) 47, 671, 960, 1032
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 6 cut(s) 95, 110, 120, 205, 1103, 1270
MspR9I CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 2 cut(s) 1430, 1552
MvaI CCWGG 4 cut(s) 638, 648, 878, 1230
MvnI CGCG 2 cut(s) 168, 1494
MwoI GCNNNNNNNGC 3 cut(s) 314, 626, 739
NciI CCSGG 2 cut(s) 95, 111
NdeII GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
NlaIII CATG 9 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1538, 1575
NlaIV GGNNCC 5 cut(s) 81, 261, 282, 324, 551
NmeAIII GCCGAG 1 cut(s) 1510
NmuCI GTSAC 1 cut(s) 1105
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 2 cut(s) 733, 1538
NspV TTCGAA 1 cut(s) 178
PaeI GCATGC 1 cut(s) 733
PciI ACATGT 1 cut(s) 1534
PcsI WCGNNNNNNNCGW 1 cut(s) 1482
PctI GAATGC 2 cut(s) 1430, 1552
PdmI GAANNNNTTC 2 cut(s) 566, 945
PfeI GAWTC 6 cut(s) 175, 180, 866, 1057, 1181, 1336
PflFI GACNNNGTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 216
PinAI ACCGGT 1 cut(s) 1102
PkrI GCNGC 2 cut(s) 481, 628
PleI GAGTC 2 cut(s) 259, 1232
PpsI GAGTC 2 cut(s) 259, 1232
Ppu21I YACGTR 2 cut(s) 76, 1333
PscI ACATGT 1 cut(s) 1534
PsiI TTATAA 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 4 cut(s) 636, 646, 876, 1228
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 4 cut(s) 636, 646, 876, 1228
PspN4I GGNNCC 5 cut(s) 81, 261, 282, 324, 551
PspPI GGNCC 6 cut(s) 79, 207, 322, 550, 1297, 1489
PsuI RGATCY 2 cut(s) 259, 1111
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 2 cut(s) 891, 1310
RsaNI GTAC 2 cut(s) 890, 1309
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 47, 671, 960, 1032
SatI GCNGC 2 cut(s) 480, 627
Sau3AI GATC 6 cut(s) 54, 229, 259, 384, 469, 1111
Sau96I GGNCC 6 cut(s) 79, 207, 322, 550, 1297, 1489
SchI GAGTC 2 cut(s) 260, 1233
ScrFI CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
SduI GDGCHC 4 cut(s) 42, 755, 1239, 1260
SfaNI GCATC 4 cut(s) 123, 1195, 1429, 1558
SfcI CTRYAG 1 cut(s) 1209
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 4 cut(s) 79, 207, 322, 1297
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 4 cut(s) 302, 1026, 1061, 1277
SmoI CTYRAG 4 cut(s) 302, 1026, 1061, 1277
SphI GCATGC 1 cut(s) 733
Sse9I AATT 8 cut(s) 380, 621, 706, 767, 855, 928, 1034, 1245
SsiI CCGC 5 cut(s) 158, 439, 806, 1295, 1492
SspMI CTAG 4 cut(s) 104, 488, 557, 863
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 6 cut(s) 93, 109, 636, 646, 876, 1228
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 4 cut(s) 925, 938, 1171, 1507
TaiI ACGT 3 cut(s) 78, 203, 1335
TaqII GACCGA 1 cut(s) 115
TasI AATT 8 cut(s) 380, 621, 706, 767, 855, 928, 1034, 1245
TfiI GAWTC 6 cut(s) 175, 180, 866, 1057, 1181, 1336
Tru1I TTAA 4 cut(s) 47, 671, 960, 1032
Tru9I TTAA 4 cut(s) 47, 671, 960, 1032
TscAI CASTG 4 cut(s) 501, 1289, 1351, 1510
TseFI GTSAC 1 cut(s) 1105
TseI GCWGC 2 cut(s) 479, 626
Tsp45I GTSAC 1 cut(s) 1105
TspDTI ATGAA 5 cut(s) 159, 559, 780, 1002, 1070
TspRI CASTG 4 cut(s) 501, 1289, 1351, 1510
Tth111I GACNNNGTC 1 cut(s) 332
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 4 cut(s) 79, 207, 322, 1297
XagI CCTNNNNNAGG 1 cut(s) 237
XapI RAATTY 4 cut(s) 706, 855, 1034, 1245
XceI RCATGY 2 cut(s) 733, 1538
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 354
XmnI GAANNNNTTC 2 cut(s) 566, 945
XspI CTAG 4 cut(s) 104, 488, 557, 863
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.