pycom05g32650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0021117
Physical Location & Seq
Forward (+)
31974677 .. 31975291
615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32650.2

Sequence Viewer

Length: 537 bp
ATGCTTTTGTCAATCTTTACCCACACCCTTGACAGGAAGTACATGTTTCTTATTTGTAATGAAATCCTTGCTTTTCTTGCAAAAACTTCGATTAATTCTTGCGGTTGCAGTTATAATATTGGGTCGTCTCTCGGCCAGGATGACATCGCTTATGTGGCTGACGAAGGAGAAGAAGAACCAACGTCGACTTCAGAAGAAGAAGAAGTTGGACAACTACAAGATCTAGAAAAACAACAAAATGGTTCGTTATATACAGAGAACCATGAAGAAATGCAGCCTGAAGCTTTCATTGCAGCAGCAGAAGATGAAGAAGAAGAAGAAGAAGAAGATGAAGAAGTCAGTCTGTCTTTGATGGAGCAAGGAGGAGAAGAAATGGAAACACAAGAGCTAGCAACAAATGTTGACGTTGGAGATGATCAGTACGGCTCGAAGATGAGCAGTACAGATGAATTGAACAAGAAATTCGAAGAGTTTATAAGAAAAATGAAGGAGGAAATCAGGATTGAAGCCCAACGCCAGCCCATTATTGCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.14

Weight (kDa)

4.05

Isoelectric Point (pI)

72.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021117)

Species Orthologous Gene IDs
malus_domestica MD00G1057200.v1.1 MD10G1337100.v1.1
prunus_persica Prupe.4G006100_v2.0.a1
pyrus_communis pycom05g32650 pycom10g28650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 114, 476
AccI GTMKAC 1 cut(s) 185
AciI CCGC 2 cut(s) 102, 531
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 1 cut(s) 461
AcuI CTGAAG 2 cut(s) 174, 300
AfaI GTAC 3 cut(s) 41, 422, 442
AfiI CCNNNNNNNGG 1 cut(s) 33
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 2 cut(s) 454, 506
AjnI CCWGG 1 cut(s) 135
AjuI GAANNNNNNNTTGG 4 cut(s) 172, 189, 204, 221
AluBI AGCT 2 cut(s) 284, 388
AluI AGCT 2 cut(s) 284, 388
Alw26I GTCTC 1 cut(s) 132
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 274, 293, 296
ApoI RAATTY 1 cut(s) 461
AseI ATTAAT 1 cut(s) 93
AsuII TTCGAA 1 cut(s) 465
AsuNHI GCTAGC 1 cut(s) 388
BbvI GCAGC 3 cut(s) 286, 305, 308
BccI CCATC 1 cut(s) 346
BceAI ACGGC 1 cut(s) 439
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciT130I CCWGG 1 cut(s) 137
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 2 cut(s) 224, 389
BglII AGATCT 1 cut(s) 220
BisI GCNGC 4 cut(s) 275, 294, 297, 531
BlsI GCNGC 4 cut(s) 276, 295, 298, 532
Bme1390I CCNGG 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 137
BmtI GCTAGC 1 cut(s) 392
Bpu14I TTCGAA 1 cut(s) 465
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 137
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 286, 305, 308
BshFI GGCC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 132
BsmBI CGTCTC 1 cut(s) 132
BsnI GGCC 1 cut(s) 135
Bsp119I TTCGAA 1 cut(s) 465
Bsp143I GATC 2 cut(s) 220, 415
BspACI CCGC 2 cut(s) 102, 531
BspANI GGCC 1 cut(s) 135
BspOI GCTAGC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 288
BssMI GATC 2 cut(s) 220, 415
Bst2UI CCWGG 1 cut(s) 137
Bst6I CTCTTC 1 cut(s) 462
BstBI TTCGAA 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 390, 518
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 2 cut(s) 223, 418
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 220, 415
BstMWI GCNNNNNNNGC 3 cut(s) 77, 155, 290
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 135
BstV1I GCAGC 3 cut(s) 286, 305, 308
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 390, 518
Csp6I GTAC 3 cut(s) 40, 421, 441
CviAII CATG 2 cut(s) 43, 263
CviJI RGCY 8 cut(s) 135, 158, 277, 284, 388, 426, 509, 520
CviKI_1 RGCY 8 cut(s) 135, 158, 277, 284, 388, 426, 509, 520
CviQI GTAC 3 cut(s) 40, 421, 441
DpnI GATC 2 cut(s) 222, 417
DpnII GATC 2 cut(s) 220, 415
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco57I CTGAAG 2 cut(s) 174, 300
EcoRII CCWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 2 cut(s) 46, 266
FaiI YATR 7 cut(s) 44, 114, 153, 250, 252, 264, 476
FatI CATG 2 cut(s) 42, 262
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 4 cut(s) 275, 294, 297, 531
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 4 cut(s) 275, 294, 297, 531
FspBI CTAG 2 cut(s) 224, 389
GluI GCNGC 4 cut(s) 275, 294, 297, 531
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 2 cut(s) 46, 266
HincII GTYRAC 2 cut(s) 186, 403
HindII GTYRAC 2 cut(s) 186, 403
HindIII AAGCTT 1 cut(s) 282
Hpy166II GTNNAC 2 cut(s) 186, 403
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 2 cut(s) 224, 499
Hpy8I GTNNAC 2 cut(s) 186, 403
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 158, 481
HpyCH4IV ACGT 2 cut(s) 182, 405
HpyCH4V TGCA 4 cut(s) 80, 108, 274, 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 155, 290
HpySE526I ACGT 2 cut(s) 182, 405
Hsp92II CATG 2 cut(s) 46, 266
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 2 cut(s) 220, 415
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 6 cut(s) 19, 122, 149, 291, 484, 530
Lsp1109I GCAGC 3 cut(s) 286, 305, 308
MaeI CTAG 2 cut(s) 224, 389
MaeII ACGT 2 cut(s) 182, 405
MalI GATC 2 cut(s) 222, 417
MboI GATC 2 cut(s) 220, 415
MflI RGATCY 1 cut(s) 220
MluCI AATT 3 cut(s) 94, 449, 461
MmeI TCCRAC 2 cut(s) 187, 388
MnlI CCTC 2 cut(s) 356, 484
MseI TTAA 2 cut(s) 93, 535
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 77, 155, 290
NdeII GATC 2 cut(s) 220, 415
NheI GCTAGC 1 cut(s) 388
NlaIII CATG 2 cut(s) 46, 266
NmeAIII GCCGAG 1 cut(s) 111
NspI RCATGY 1 cut(s) 46
NspV TTCGAA 1 cut(s) 465
PciI ACATGT 1 cut(s) 42
PkrI GCNGC 4 cut(s) 276, 295, 298, 532
PscI ACATGT 1 cut(s) 42
PshBI ATTAAT 1 cut(s) 93
PsiI TTATAA 2 cut(s) 114, 476
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 3 cut(s) 41, 422, 442
RsaNI GTAC 3 cut(s) 40, 421, 441
SalI GTCGAC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 93, 535
SatI GCNGC 4 cut(s) 275, 294, 297, 531
Sau3AI GATC 2 cut(s) 220, 415
ScrFI CCNGG 1 cut(s) 137
SetI ASST 4 cut(s) 185, 286, 390, 408
SfuI TTCGAA 1 cut(s) 465
Sse9I AATT 3 cut(s) 94, 449, 461
SsiI CCGC 2 cut(s) 102, 531
SspI AATATT 1 cut(s) 118
SspMI CTAG 2 cut(s) 224, 389
StyD4I CCNGG 1 cut(s) 135
TaiI ACGT 2 cut(s) 185, 408
TaqI TCGA 4 cut(s) 89, 185, 428, 465
TasI AATT 3 cut(s) 94, 449, 461
TatI WGTACW 2 cut(s) 39, 440
TauI GCSGC 1 cut(s) 533
Tru1I TTAA 2 cut(s) 93, 535
Tru9I TTAA 2 cut(s) 93, 535
TseI GCWGC 3 cut(s) 274, 293, 296
TspDTI ATGAA 7 cut(s) 75, 277, 279, 321, 345, 462, 500
VspI ATTAAT 1 cut(s) 93
XapI RAATTY 1 cut(s) 461
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 185
XspI CTAG 2 cut(s) 224, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.