pycom06g04250

Phospholipase A1-Ibeta2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014430
Physical Location & Seq
Reverse (-)
5336564 .. 5338033
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1470 bp
ATGCTCGTCGGATCAACGCTCCCAGCTCACAACCTCAACCTGTTTCCGGCCAGAAGAGCCAACTTCAGAAACTACACGTCAGCATTAAACCCGTCAACGAGAGCTCCATCCTCTTGTTCTTATTCAGCTGTCTCCATAAAACCCGGTTCAAACTCCACCGAGTTGACTCGGCTCCACCTCGCCAACCTCGACAAGCTCCTCCAAACCCCACCTCCGCCCCAAACCCAACTCGACTCACAACCCGATATCCAAATAGACAAAGACCCGAATGATAGTGGGTCGCTCGAAAACAAGAGAAGGAGGCTTATGGAAGGGCTCAATCTTTCTCAGTTGTGGACGCAAGTGAAAGGCGCCGCCGAAGACATGTCTCCGAGACACCTCAATCGCCTGCAGCGCCTCCTGTCCAAGACTGAAGAGTACTCTCCCAGAAATAATCTCAGCACCCGGTGGCGCGAGTATCACGGTTCAAACAACTGGGCCGGACTACTTGACCCGCTGGATGAGAATCTCCGGCGAGAGGTCGTCAGATACGGTGAGTTTATCCAGGCGGCTTATCATTCCTTTCATTCAAACCCGGCCATGTCGGCAGAGGAGGCTCAGGTGCCTCGCCAAGTGGCGCTTCCCGATAGGTCTTATAAGGTAACCAAGAGCCTTTACGCCACGTCATCAATCGGGTTGCCCGATTGGGTCGATGAAGCGAGGCCGGATCTCGGTTGGATGACCCAGCGGTCGAGCTGGGTTGGGTACGTGGCCGTTTGTGATGATAAGAGGGAGATCGCACGGATGGGCAGACGCGACATTGTCATTGCCCTCCGTGGAACCTCCACGTGTCTTGAATGGGCGGAGAATTTAAGGGCTCAGCTCATTCAGATGCCCTCGACCGAGGAAGGTGAGCAAAGGCAAGCAAGAGTGGAATGCGGATTTTTGAGCCTCTACAAAACACGCGGGGCTCACGTACCGAGCCTTGCCGAGTCGGTGGTTGAAGAGGTCAGGAGGCTGATGGAGCAATACAAAGGAGAGACATTAAGCATAACTGTGACAGGACACAGCCTTGGTGCTGCCTTAGCCTTGTTAGTAGGCGATGAGCTTAAAACATGCGCCGAGAACATGCCGTCGGTGGCGGTTTTCTCCTTTGGAGGCCCCCGCGTAGGTAACCGAGGATTCACAAAGCGAATGGACGACAAGAATGTCAAGGTATTGAGGATTGTCAACTCCCAAGATGTGATCACGAGGGTTCCGGGTATTTTCGTAGGCGAAGGAGTACTCGAGGAGAAGCTGAACAACGCAAAGGCCGGAGGATTCATTGACATGATCGACAGGAACATGCCGTTGGCTTACTCACATGTTGGGACAGAACTACGGGTTGACACAAAAATGTCGCCTTACTTGAAGCCCAACGCAGATGTGGCTTGCTGCCATGATTTGGAGGCATATTTGCATTTGGTGGATGGGTTTTTGACATCTAACTGA

Protein Analysis

490

Amino Acids

54.6

Weight (kDa)

7.17

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 268 - 415 3.3e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16820
fragaria_vesca FvH4_5g19980
malus_domestica MD04G1049800.v1.1 MD06G1050100.v1.1
prunus_persica Prupe.5G060400_v2.0.a1
pyrus_communis pycom04g04380 pycom06g04250
rosa_chinensis RchiOBHm_Chr7g0204811
rosa_laevigata RLG00000003431
rosa_multiflora Rmu_sc0003221.1_g000039
rosa_roxburghii Rroxscaffold_3G00252770
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216100 Rh7BG213200 Rh7CG229400 Rh7DG223800
rosa_wichuraiana Rw7G018740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AasI GACNNNNNNGTC 2 cut(s) 727, 1187
AccB1I GGYRCC 2 cut(s) 350, 601
AccB7I CCANNNNNTGG 1 cut(s) 1423
AccII CGCG 4 cut(s) 453, 795, 945, 1146
AclWI GGATC 2 cut(s) 19, 714
AcoI YGGCCR 3 cut(s) 48, 576, 750
AcsI RAATTY 1 cut(s) 847
AcuI CTGAAG 2 cut(s) 49, 432
AcvI CACGTG 1 cut(s) 828
AcyI GRCGYC 1 cut(s) 351
AdeI CACNNNGTG 1 cut(s) 447
AfaI GTAC 4 cut(s) 419, 746, 957, 1263
AfiI CCNNNNNNNGG 5 cut(s) 46, 517, 710, 1148, 1423
AflIII ACRYGT 4 cut(s) 75, 363, 827, 1342
AgsI TTSAA 6 cut(s) 150, 468, 570, 836, 983, 1390
AjiI CACGTC 2 cut(s) 78, 663
AjnI CCWGG 1 cut(s) 543
AjuI GAANNNNNNNTTGG 4 cut(s) 603, 635, 1313, 1345
AluBI AGCT 8 cut(s) 26, 104, 128, 196, 735, 862, 1087, 1276
AluI AGCT 8 cut(s) 26, 104, 128, 196, 735, 862, 1087, 1276
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 4 cut(s) 136, 367, 372, 1013
AlwI GGATC 2 cut(s) 19, 714
Ama87I CYCGRG 1 cut(s) 1265
AoxI GGCC 7 cut(s) 48, 477, 576, 701, 750, 1138, 1290
ApeKI GCWGC 3 cut(s) 391, 1058, 1413
ApoI RAATTY 1 cut(s) 847
AspLEI GCGC 5 cut(s) 353, 396, 453, 619, 1100
AspS9I GGNCC 2 cut(s) 477, 1139
AsuC2I CCSGG 4 cut(s) 144, 445, 575, 1239
AsuHPI GGTGA 2 cut(s) 545, 902
AvaI CYCGRG 1 cut(s) 1265
BanI GGYRCC 2 cut(s) 350, 601
BanII GRGCYC 4 cut(s) 106, 318, 859, 952
BauI CACGAG 1 cut(s) 1228
BbrPI CACGTG 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 3 cut(s) 403, 1045, 1400
BccI CCATC 4 cut(s) 115, 778, 994, 1442
BceAI ACGGC 3 cut(s) 737, 1096, 1312
BciT130I CCWGG 1 cut(s) 545
BclI TGATCA 1 cut(s) 1224
BcnI CCSGG 4 cut(s) 144, 445, 575, 1239
BcoDI GTCTC 4 cut(s) 136, 367, 372, 1013
BfmI CTRYAG 1 cut(s) 389
BfoI RGCGCY 3 cut(s) 354, 397, 620
BglI GCCNNNNNGGC 1 cut(s) 584
BisI GCNGC 5 cut(s) 354, 392, 549, 1059, 1414
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 5 cut(s) 355, 393, 550, 1060, 1415
BmcAI AGTACT 2 cut(s) 419, 1263
Bme1390I CCNGG 5 cut(s) 144, 445, 545, 575, 1239
BmeT110I CYCGRG 1 cut(s) 1265
BmgBI CACGTC 2 cut(s) 78, 663
BmgT120I GGNCC 2 cut(s) 477, 1139
BmiI GGNNCC 6 cut(s) 173, 352, 603, 820, 1141, 1236
BmrFI CCNGG 5 cut(s) 144, 445, 545, 575, 1239
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 1 cut(s) 861
BmuI ACTGGG 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 366
Bpu10I CCTNAGC 2 cut(s) 597, 1063
Bpu1102I GCTNAGC 1 cut(s) 858
BpuMI CCSGG 4 cut(s) 144, 445, 575, 1239
BsaAI YACGTR 3 cut(s) 748, 828, 955
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 814, 882, 1051, 1156
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 196, 226
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 517, 710, 1148, 1423
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 4 cut(s) 814, 882, 1051, 1156
BseGI GGATG 5 cut(s) 107, 505, 723, 789, 1453
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 5 cut(s) 46, 517, 710, 1148, 1423
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 4 cut(s) 341, 451, 611, 872
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 3 cut(s) 188, 605, 1283
BseXI GCAGC 3 cut(s) 403, 1045, 1400
BseYI CCCAGC 3 cut(s) 22, 723, 735
Bsh1236I CGCG 4 cut(s) 453, 795, 945, 1146
Bsh1285I CGRYCG 2 cut(s) 731, 882
BshFI GGCC 7 cut(s) 50, 479, 578, 703, 752, 1140, 1292
BshNI GGYRCC 2 cut(s) 350, 601
BsiEI CGRYCG 2 cut(s) 731, 882
BsiHKAI GWGCWC 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 1265
BsiSI CCGG 9 cut(s) 47, 144, 445, 480, 511, 575, 704, 1238, 1293
BslFI GGGAC 1 cut(s) 1363
BslI CCNNNNNNNGG 5 cut(s) 46, 517, 710, 1148, 1423
BsmAI GTCTC 4 cut(s) 136, 367, 372, 1013
BsmFI GGGAC 1 cut(s) 1363
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 7 cut(s) 50, 479, 578, 703, 752, 1140, 1292
BsoBI CYCGRG 1 cut(s) 1265
Bsp1286I GDGCHC 4 cut(s) 106, 318, 859, 952
Bsp143I GATC 5 cut(s) 11, 706, 774, 1224, 1311
Bsp1720I GCTNAGC 1 cut(s) 858
BspANI GGCC 7 cut(s) 50, 479, 578, 703, 752, 1140, 1292
BspCNI CTCAG 4 cut(s) 340, 450, 610, 871
BspFNI CGCG 4 cut(s) 453, 795, 945, 1146
BspLI GGNNCC 6 cut(s) 173, 352, 603, 820, 1141, 1236
BspMAI CTGCAG 1 cut(s) 393
BspPI GGATC 2 cut(s) 19, 714
BspQI GCTCTTC 1 cut(s) 49
BspT107I GGYRCC 2 cut(s) 350, 601
BsrDI GCAATG 1 cut(s) 804
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 4 cut(s) 814, 882, 1051, 1156
BssMI GATC 5 cut(s) 11, 706, 774, 1224, 1311
BssNI GRCGYC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 1228
BssT1I CCWWGG 1 cut(s) 1051
Bst2BI CACGAG 1 cut(s) 1228
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 3 cut(s) 464, 533, 1036
Bst6I CTCTTC 3 cut(s) 49, 408, 978
BstACI GRCGYC 1 cut(s) 351
BstBAI YACGTR 3 cut(s) 748, 828, 955
BstC8I GCNNGC 3 cut(s) 389, 903, 1411
BstDEI CTNAG 5 cut(s) 327, 437, 597, 858, 1063
BstDSI CCRYGG 1 cut(s) 814
BstEII GGTNACC 2 cut(s) 640, 1151
BstF5I GGATG 5 cut(s) 107, 505, 723, 789, 1453
BstFNI CGCG 4 cut(s) 453, 795, 945, 1146
BstH2I RGCGCY 3 cut(s) 354, 397, 620
BstHHI GCGC 5 cut(s) 353, 396, 453, 619, 1100
BstKTI GATC 5 cut(s) 14, 709, 777, 1227, 1314
BstMAI GTCTC 4 cut(s) 136, 367, 372, 1013
BstMBI GATC 5 cut(s) 11, 706, 774, 1224, 1311
BstMCI CGRYCG 2 cut(s) 731, 882
BstMWI GCNNNNNNNGC 7 cut(s) 56, 393, 584, 593, 1003, 1064, 1406
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 5 cut(s) 367, 1098, 1111, 1327, 1346
BstPI GGTNACC 2 cut(s) 640, 1151
BstSCI CCNGG 5 cut(s) 142, 443, 543, 573, 1237
BstSFI CTRYAG 1 cut(s) 389
BstUI CGCG 4 cut(s) 453, 795, 945, 1146
BstV1I GCAGC 3 cut(s) 403, 1045, 1400
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
BsuRI GGCC 7 cut(s) 50, 479, 578, 703, 752, 1140, 1292
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 1 cut(s) 1095
BtrI CACGTC 2 cut(s) 78, 663
BtsCI GGATG 5 cut(s) 107, 505, 723, 789, 1453
Cac8I GCNNGC 3 cut(s) 389, 903, 1411
CfoI GCGC 5 cut(s) 353, 396, 453, 619, 1100
Cfr13I GGNCC 2 cut(s) 477, 1139
CseI GACGC 2 cut(s) 346, 801
Csp6I GTAC 4 cut(s) 418, 745, 956, 1262
CspCI CAANNNNNGTGG 2 cut(s) 814, 849
CviAII CATG 8 cut(s) 364, 580, 1095, 1108, 1309, 1324, 1343, 1418
CviQI GTAC 4 cut(s) 418, 745, 956, 1262
DdeI CTNAG 5 cut(s) 327, 437, 597, 858, 1063
DinI GGCGCC 1 cut(s) 352
DpnI GATC 5 cut(s) 13, 708, 776, 1226, 1313
DpnII GATC 5 cut(s) 11, 706, 774, 1224, 1311
DraIII CACNNNGTG 1 cut(s) 447
DrdI GACNNNNNNGTC 2 cut(s) 727, 1187
DseDI GACNNNNNNGTC 2 cut(s) 727, 1187
EaeI YGGCCR 3 cut(s) 48, 576, 750
Eam1104I CTCTTC 3 cut(s) 49, 408, 978
EarI CTCTTC 3 cut(s) 49, 408, 978
EciI GGCGGA 2 cut(s) 204, 857
Ecl136II GAGCTC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 1051
Eco24I GRGCYC 4 cut(s) 106, 318, 859, 952
Eco32I GATATC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 104
Eco57I CTGAAG 2 cut(s) 49, 432
Eco72I CACGTG 1 cut(s) 828
Eco88I CYCGRG 1 cut(s) 1265
Eco91I GGTNACC 2 cut(s) 640, 1151
EcoICRI GAGCTC 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 1139
EcoO65I GGTNACC 2 cut(s) 640, 1151
EcoRII CCWGG 1 cut(s) 543
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 1051
EcoT38I GRGCYC 4 cut(s) 106, 318, 859, 952
EgeI GGCGCC 1 cut(s) 352
EheI GGCGCC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 1051
FaeI CATG 8 cut(s) 367, 583, 1098, 1111, 1312, 1327, 1346, 1421
FalI AAGNNNNNCTT 2 cut(s) 603, 635
FaqI GGGAC 1 cut(s) 1363
FatI CATG 8 cut(s) 363, 579, 1094, 1107, 1308, 1323, 1342, 1417
FauI CCCGC 3 cut(s) 501, 938, 1151
FbaI TGATCA 1 cut(s) 1224
Fnu4HI GCNGC 5 cut(s) 354, 392, 549, 1059, 1414
FokI GGATG 5 cut(s) 94, 512, 730, 796, 1460
FriOI GRGCYC 4 cut(s) 106, 318, 859, 952
Fsp4HI GCNGC 5 cut(s) 354, 392, 549, 1059, 1414
GlaI GCGC 5 cut(s) 352, 395, 452, 618, 1099
GluI GCNGC 5 cut(s) 354, 392, 549, 1059, 1414
GsaI CCCAGC 3 cut(s) 26, 727, 739
HaeII RGCGCY 3 cut(s) 354, 397, 620
HaeIII GGCC 7 cut(s) 50, 479, 578, 703, 752, 1140, 1292
HapII CCGG 9 cut(s) 47, 144, 445, 480, 511, 575, 704, 1238, 1293
HgaI GACGC 2 cut(s) 346, 801
HhaI GCGC 5 cut(s) 353, 396, 453, 619, 1100
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 8 cut(s) 367, 583, 1098, 1111, 1312, 1327, 1346, 1421
Hin6I GCGC 5 cut(s) 351, 394, 451, 617, 1098
HinP1I GCGC 5 cut(s) 351, 394, 451, 617, 1098
HincII GTYRAC 4 cut(s) 96, 165, 1210, 1366
HindII GTYRAC 4 cut(s) 96, 165, 1210, 1366
HinfI GANTC 6 cut(s) 166, 233, 505, 971, 1161, 1299
HpaII CCGG 9 cut(s) 47, 144, 445, 480, 511, 575, 704, 1238, 1293
HphI GGTGA 2 cut(s) 545, 902
Hpy166II GTNNAC 5 cut(s) 96, 165, 336, 1210, 1366
Hpy188I TCNGA 5 cut(s) 11, 68, 372, 527, 870
Hpy188III TCNNGA 4 cut(s) 623, 833, 991, 1228
Hpy8I GTNNAC 5 cut(s) 96, 165, 336, 1210, 1366
Hpy99I CGWCG 2 cut(s) 11, 1117
HpyAV CCTTC 4 cut(s) 291, 305, 881, 1250
HpyCH4III ACNGT 3 cut(s) 464, 533, 1036
HpyCH4IV ACGT 5 cut(s) 77, 662, 747, 827, 954
HpyCH4V TGCA 2 cut(s) 391, 1438
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 393, 584, 593, 1003, 1064, 1406
HpyF3I CTNAG 5 cut(s) 327, 437, 597, 858, 1063
HpySE526I ACGT 5 cut(s) 77, 662, 747, 827, 954
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 8 cut(s) 367, 583, 1098, 1111, 1312, 1327, 1346, 1421
HspAI GCGC 5 cut(s) 351, 394, 451, 617, 1098
KasI GGCGCC 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 1224
Kzo9I GATC 5 cut(s) 11, 706, 774, 1224, 1311
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 5 cut(s) 24, 109, 177, 201, 1003
Lsp1109I GCAGC 3 cut(s) 403, 1045, 1400
LweI GCATC 1 cut(s) 861
MaeII ACGT 5 cut(s) 77, 662, 747, 827, 954
MaeIII GTNAC 3 cut(s) 640, 1036, 1151
MalI GATC 5 cut(s) 13, 708, 776, 1226, 1313
MboI GATC 5 cut(s) 11, 706, 774, 1224, 1311
MboII GAAGA 4 cut(s) 66, 371, 425, 995
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 4 cut(s) 106, 318, 859, 952
MluCI AATT 1 cut(s) 847
Mly113I GGCGCC 1 cut(s) 351
MlyI GAGTC 3 cut(s) 160, 227, 980
MmeI TCCRAC 1 cut(s) 695
MseI TTAA 4 cut(s) 86, 851, 1025, 1089
MslI CAYNNNNRTG 4 cut(s) 869, 1034, 1307, 1373
MspA1I CMGCKG 3 cut(s) 128, 496, 727
MspI CCGG 9 cut(s) 47, 144, 445, 480, 511, 575, 704, 1238, 1293
MspR9I CCNGG 5 cut(s) 144, 445, 545, 575, 1239
Mva1269I GAATGC 1 cut(s) 920
MvaI CCWGG 1 cut(s) 545
MvnI CGCG 4 cut(s) 453, 795, 945, 1146
MwoI GCNNNNNNNGC 7 cut(s) 56, 393, 584, 593, 1003, 1064, 1406
NarI GGCGCC 1 cut(s) 351
NciI CCSGG 4 cut(s) 144, 445, 575, 1239
NdeII GATC 5 cut(s) 11, 706, 774, 1224, 1311
NlaIII CATG 8 cut(s) 367, 583, 1098, 1111, 1312, 1327, 1346, 1421
NlaIV GGNNCC 6 cut(s) 173, 352, 603, 820, 1141, 1236
NmeAIII GCCGAG 3 cut(s) 148, 994, 1126
NmuCI GTSAC 1 cut(s) 1036
NspI RCATGY 5 cut(s) 367, 1098, 1111, 1327, 1346
PaeR7I CTCGAG 1 cut(s) 1265
PciI ACATGT 2 cut(s) 363, 1342
PciSI GCTCTTC 1 cut(s) 49
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 678
PctI GAATGC 1 cut(s) 920
PfeI GAWTC 3 cut(s) 505, 1161, 1299
PflFI GACNNNGTC 1 cut(s) 800
PflMI CCANNNNNTGG 1 cut(s) 1423
PkrI GCNGC 5 cut(s) 355, 393, 550, 1060, 1415
PleI GAGTC 3 cut(s) 160, 227, 979
PluTI GGCGCC 1 cut(s) 354
PmaCI CACGTG 1 cut(s) 828
PmlI CACGTG 1 cut(s) 828
PpsI GAGTC 3 cut(s) 160, 227, 979
Ppu21I YACGTR 3 cut(s) 748, 828, 955
PscI ACATGT 2 cut(s) 363, 1342
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 543
PspCI CACGTG 1 cut(s) 828
PspEI GGTNACC 2 cut(s) 640, 1151
PspFI CCCAGC 3 cut(s) 22, 723, 735
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 6 cut(s) 173, 352, 603, 820, 1141, 1236
PspPI GGNCC 2 cut(s) 477, 1139
PspXI VCTCGAGB 1 cut(s) 1265
PstI CTGCAG 1 cut(s) 393
PsuI RGATCY 1 cut(s) 706
PsyI GACNNNGTC 1 cut(s) 800
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 4 cut(s) 419, 746, 957, 1263
RsaNI GTAC 4 cut(s) 418, 745, 956, 1262
RseI CAYNNNNRTG 4 cut(s) 869, 1034, 1307, 1373
SacI GAGCTC 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 4 cut(s) 86, 851, 1025, 1089
SatI GCNGC 5 cut(s) 354, 392, 549, 1059, 1414
Sau3AI GATC 5 cut(s) 11, 706, 774, 1224, 1311
Sau96I GGNCC 2 cut(s) 477, 1139
ScaI AGTACT 2 cut(s) 419, 1263
SchI GAGTC 3 cut(s) 160, 227, 980
ScrFI CCNGG 5 cut(s) 144, 445, 545, 575, 1239
SduI GDGCHC 4 cut(s) 106, 318, 859, 952
SfaNI GCATC 1 cut(s) 861
SfcI CTRYAG 1 cut(s) 389
SfoI GGCGCC 1 cut(s) 352
Sfr274I CTCGAG 1 cut(s) 1265
SlaI CTCGAG 1 cut(s) 1265
SmiMI CAYNNNNRTG 4 cut(s) 869, 1034, 1307, 1373
SmlI CTYRAG 1 cut(s) 1265
SmoI CTYRAG 1 cut(s) 1265
Sse9I AATT 1 cut(s) 847
SspDI GGCGCC 1 cut(s) 350
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 5 cut(s) 142, 443, 543, 573, 1237
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 3 cut(s) 464, 533, 1036
TaiI ACGT 5 cut(s) 80, 665, 750, 830, 957
TaqI TCGA 8 cut(s) 189, 231, 285, 690, 731, 878, 1266, 1314
TaqII GACCGA 1 cut(s) 896
TasI AATT 1 cut(s) 847
TatI WGTACW 2 cut(s) 417, 1261
TauI GCSGC 2 cut(s) 356, 551
TfiI GAWTC 3 cut(s) 505, 1161, 1299
Tru1I TTAA 4 cut(s) 86, 851, 1025, 1089
Tru9I TTAA 4 cut(s) 86, 851, 1025, 1089
TseFI GTSAC 1 cut(s) 1036
TseI GCWGC 3 cut(s) 391, 1058, 1413
Tsp45I GTSAC 1 cut(s) 1036
TspDTI ATGAA 3 cut(s) 554, 708, 1291
TspGWI ACGGA 2 cut(s) 796, 803
Tth111I GACNNNGTC 1 cut(s) 800
Van91I CCANNNNNTGG 1 cut(s) 1423
XapI RAATTY 1 cut(s) 847
XceI RCATGY 5 cut(s) 367, 1098, 1111, 1327, 1346
XcmI CCANNNNNNNNNTGG 1 cut(s) 1402
XhoI CTCGAG 1 cut(s) 1265
ZrmI AGTACT 2 cut(s) 419, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.