pycom07g05490

Glucomannan 4-beta-mannosyltransferase 9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0022545
Physical Location & Seq
Reverse (-)
4776887 .. 4778797
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGAGGCAATGCAAGGAGGGCAAATTGGGTTGGTTTTGCAGCAAGCTAAGGCGCCGCTAATCGTACCGTTGCTGAAGTTGGTGGTGGTTTTGTGCTTGGGTATGTCTGTGATGCTTTTTGTGGAGAAGGTGTACATGGGCATTGTCATAACGTTGATTAAGCTGTTTAGAAGAAAGCCTGAGAAGAAGTGGAAATGGGAGGCCGTTAAGGATGATGTTGAGCTTGGAAACTCAGCTTATCCTATGGTTCTTGTGCAAATTCCAATGTACAATGAAAAAGAGGTTTACCAGCTATCAATTGGAGCGGCATGTGGACTTTCCTGGCCATCTGACAGGATTATCATTCAAGTTCTGGATGATTCAACAGATCCAGCCATCAAGGATTTGGTGGAACTCGAAGTGCAAAGATGGGCAAGCAAAGGTATAAACATAAGGTATGAGATCAGAGGCAACAGAAATGGGTACAAGGCAGGGGCTCTGAAAGAAGGAATGAAGCACAGTTATGTCAAACACTGTGATTATGTTGCCATCTTTGATGCTGACTTCCAACCTGAGCCTGATTTTCTCTGGCGCACCATTCCATTTCTCATCCACAACCCTGAAGTTGGTCTTGTCCAAACCCGTTGGAAATTTGGTAATCATCGTTATTTCTTCTTAATTACTATTATTATCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3

Protein Analysis

226

Amino Acids

25.83

Weight (kDa)

8.65

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_2 PF00535 86 - 216 1.1e-11 Glycosyl transferase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022545)

Species Orthologous Gene IDs
pyrus_communis pycom07g05490
rosa_chinensis RchiOBHm_Chr5g0055371
rosa_multiflora Rmu_sc0001673.1_g000013 Rmu_sc0018900.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 52
AccBSI CCGCTC 1 cut(s) 305
AciI CCGC 2 cut(s) 56, 305
AclI AACGTT 1 cut(s) 152
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 2 cut(s) 258, 629
AcuI CTGAAG 2 cut(s) 95, 621
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 4 cut(s) 66, 134, 269, 464
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 2 cut(s) 347, 363
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 5 cut(s) 47, 163, 223, 236, 292
AluI AGCT 5 cut(s) 47, 163, 223, 236, 292
AlwI GGATC 1 cut(s) 362
AoxI GGCC 2 cut(s) 201, 323
ApeKI GCWGC 1 cut(s) 40
ApoI RAATTY 2 cut(s) 258, 629
AspLEI GCGC 2 cut(s) 55, 573
BalI TGGCCA 1 cut(s) 325
BanI GGYRCC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 52
BccI CCATC 4 cut(s) 334, 383, 402, 536
BceAI ACGGC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 3 cut(s) 41, 56, 306
BlsI GCNGC 3 cut(s) 42, 57, 307
Bme1390I CCNGG 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 2 cut(s) 102, 526
Bpu10I CCTNAGC 2 cut(s) 48, 552
BsaHI GRCGYC 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 1 cut(s) 322
BseGI GGATG 3 cut(s) 217, 361, 588
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 171, 246, 543
BseXI GCAGC 1 cut(s) 52
BshFI GGCC 2 cut(s) 203, 325
BshNI GGYRCC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 605
BsnI GGCC 2 cut(s) 203, 325
Bsp1286I GDGCHC 1 cut(s) 478
Bsp1407I TGTACA 2 cut(s) 132, 267
Bsp143I GATC 2 cut(s) 367, 441
BspACI CCGC 2 cut(s) 56, 305
BspANI GGCC 2 cut(s) 203, 325
BspCNI CTCAG 3 cut(s) 172, 245, 544
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 52
BsrBI CCGCTC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 15
BsrGI TGTACA 2 cut(s) 132, 267
BssMI GATC 2 cut(s) 367, 441
BssNI GRCGYC 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 3 cut(s) 69, 500, 515
BstACI GRCGYC 1 cut(s) 53
BstAUI TGTACA 2 cut(s) 132, 267
BstC8I GCNNGC 2 cut(s) 45, 415
BstDEI CTNAG 4 cut(s) 48, 180, 232, 552
BstF5I GGATG 3 cut(s) 217, 361, 588
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 2 cut(s) 55, 573
BstKTI GATC 2 cut(s) 370, 444
BstMBI GATC 2 cut(s) 367, 441
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 2 cut(s) 203, 325
BtsCI GGATG 3 cut(s) 217, 361, 588
BtsIMutI CAGTG 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 45, 415
CfoI GCGC 2 cut(s) 55, 573
Csp6I GTAC 4 cut(s) 65, 133, 268, 463
CviAII CATG 2 cut(s) 136, 309
CviQI GTAC 4 cut(s) 65, 133, 268, 463
DdeI CTNAG 4 cut(s) 48, 180, 232, 552
DinI GGCGCC 1 cut(s) 54
DpnI GATC 2 cut(s) 369, 443
DpnII GATC 2 cut(s) 367, 441
EaeI YGGCCR 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 478
Eco57I CTGAAG 2 cut(s) 95, 621
EcoRII CCWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 478
EgeI GGCGCC 1 cut(s) 54
EheI GGCGCC 1 cut(s) 54
FaeI CATG 2 cut(s) 139, 312
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FatI CATG 2 cut(s) 135, 308
Fnu4HI GCNGC 3 cut(s) 41, 56, 306
FokI GGATG 3 cut(s) 224, 368, 575
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 3 cut(s) 41, 56, 306
GlaI GCGC 2 cut(s) 54, 572
GluI GCNGC 3 cut(s) 41, 56, 306
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 203, 325
HhaI GCGC 2 cut(s) 55, 573
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 2 cut(s) 139, 312
Hin6I GCGC 2 cut(s) 53, 571
HinP1I GCGC 2 cut(s) 53, 571
HinfI GANTC 1 cut(s) 359
Hpy166II GTNNAC 3 cut(s) 133, 286, 314
Hpy188I TCNGA 3 cut(s) 331, 446, 480
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 3 cut(s) 133, 286, 314
HpyAV CCTTC 2 cut(s) 121, 479
HpyCH4III ACNGT 3 cut(s) 69, 500, 515
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 4 cut(s) 13, 40, 256, 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 4 cut(s) 48, 180, 232, 552
HpySE526I ACGT 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 2 cut(s) 139, 312
HspAI GCGC 2 cut(s) 53, 571
KasI GGCGCC 1 cut(s) 52
Kzo9I GATC 2 cut(s) 367, 441
LmnI GCTCC 1 cut(s) 302
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 2 cut(s) 102, 526
MaeII ACGT 1 cut(s) 152
MalI GATC 2 cut(s) 369, 443
MbiI CCGCTC 1 cut(s) 305
MboI GATC 2 cut(s) 367, 441
MboII GAAGA 3 cut(s) 183, 196, 643
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 478
MlsI TGGCCA 1 cut(s) 325
MluCI AATT 5 cut(s) 24, 258, 297, 629, 657
MluNI TGGCCA 1 cut(s) 325
Mly113I GGCGCC 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 571, 605
MnlI CCTC 4 cut(s) 11, 193, 274, 440
Mox20I TGGCCA 1 cut(s) 325
MscI TGGCCA 1 cut(s) 325
MseI TTAA 3 cut(s) 159, 207, 656
MslI CAYNNNNRTG 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 325
MspR9I CCNGG 1 cut(s) 322
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 19
NarI GGCGCC 1 cut(s) 53
NdeII GATC 2 cut(s) 367, 441
NlaIII CATG 2 cut(s) 139, 312
NlaIV GGNNCC 1 cut(s) 54
NspI RCATGY 1 cut(s) 312
PfeI GAWTC 1 cut(s) 359
PkrI GCNGC 3 cut(s) 42, 57, 307
PluTI GGCGCC 1 cut(s) 56
Psp1406I AACGTT 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 54
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 4 cut(s) 66, 134, 269, 464
RsaNI GTAC 4 cut(s) 65, 133, 268, 463
RseI CAYNNNNRTG 1 cut(s) 501
SaqAI TTAA 3 cut(s) 159, 207, 656
SatI GCNGC 3 cut(s) 41, 56, 306
Sau3AI GATC 2 cut(s) 367, 441
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 1 cut(s) 478
SfaNI GCATC 2 cut(s) 102, 526
SfoI GGCGCC 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 501
Sse9I AATT 5 cut(s) 24, 258, 297, 629, 657
SsiI CCGC 2 cut(s) 56, 305
SspDI GGCGCC 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 69, 500, 515
TaiI ACGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 396
TasI AATT 5 cut(s) 24, 258, 297, 629, 657
TatI WGTACW 2 cut(s) 132, 267
TauI GCSGC 2 cut(s) 58, 308
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 3 cut(s) 159, 207, 656
Tru9I TTAA 3 cut(s) 159, 207, 656
TscAI CASTG 1 cut(s) 518
TseI GCWGC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 288, 506
TspRI CASTG 1 cut(s) 518
XapI RAATTY 2 cut(s) 258, 629
XceI RCATGY 1 cut(s) 312
XcmI CCANNNNNNNNNTGG 2 cut(s) 296, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.