Rmu_sc0001673.1_g000013

Glucomannan 4-beta-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001673.1
OG0022545
Physical Location & Seq
Reverse (-)
54180 .. 55210
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001673.1_g000013.1.cds

Sequence Viewer

Length: 654 bp
atggctgagagtaccaaagttctgataccagagacattccagggtgggagtagctttgacattgcaggccaaattgggctgatttgggagctaatcaaagcgccattgatagttcctctgcttagagttggagtctatgtctcactagccatggttctcatgctctttgtagagagggtctacatgggtattgtcattgttttggtcaaaatgttctggaaaaagccagagaagcgctacaactatgagcccattcaggaggatttgggattgggtagttccaatttcccaatggtcttcaagatctccattggagctgcatgtgggctgtcgtggccgtccgatcgtcttgtgatccaagtcctggatgattcaactgaccctacaatcaagaaaatggtggaggcagagtgtcagaggtgggcgagaaaaggcataaacatagtgtaccaaatcagagaaactagaggaggatacaaagcaggggcattgaaagaaggtctgaaacgaagctacgttaaacactgcgagtacgtggctataatggacgccgatttccgacccgagccagactatctcaggcgagccatccctttcttggttcagaaccccgaaattgctttggttcaagcacgttggagattcggtaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3 Glycos_transf_2

Protein Analysis

217

Amino Acids

24.66

Weight (kDa)

9.06

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022545)

Species Orthologous Gene IDs
pyrus_communis pycom07g05490
rosa_chinensis RchiOBHm_Chr5g0055371
rosa_multiflora Rmu_sc0001673.1_g000013 Rmu_sc0018900.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 364
AccI GTMKAC 1 cut(s) 180
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 3 cut(s) 13, 449, 533
AfeI AGCGCT 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 364, 598
AgsI TTSAA 4 cut(s) 301, 375, 493, 629
AjnI CCWGG 2 cut(s) 39, 363
AluBI AGCT 4 cut(s) 54, 91, 317, 513
AluI AGCT 4 cut(s) 54, 91, 317, 513
Alw26I GTCTC 2 cut(s) 26, 145
AlwI GGATC 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 563
Aor51HI AGCGCT 1 cut(s) 236
AoxI GGCC 2 cut(s) 67, 335
ApeKI GCWGC 1 cut(s) 317
AspLEI GCGC 2 cut(s) 103, 237
AvaI CYCGRG 1 cut(s) 563
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 304
BccI CCATC 1 cut(s) 596
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 2 cut(s) 41, 365
BciVI GTATCC 1 cut(s) 467
BcoDI GTCTC 2 cut(s) 26, 145
BfaI CTAG 2 cut(s) 146, 465
BfoI RGCGCY 2 cut(s) 104, 238
BfuI GTATCC 1 cut(s) 467
BglII AGATCT 1 cut(s) 303
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
Bme1390I CCNGG 2 cut(s) 41, 365
BmeT110I CYCGRG 1 cut(s) 563
BmrFI CCNGG 2 cut(s) 41, 365
BpiI GAAGAC 1 cut(s) 289
BsaAI YACGTR 1 cut(s) 535
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 40, 150
BsaXI ACNNNNNCTCC 5 cut(s) 123, 153, 395, 425, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 364, 598
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 41, 365
BseDI CCNNGG 2 cut(s) 40, 150
BseGI GGATG 2 cut(s) 373, 588
BseLI CCNNNNNNNGG 2 cut(s) 364, 598
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 592
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 2 cut(s) 69, 337
BsiEI CGRYCG 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 563
BslI CCNNNNNNNGG 2 cut(s) 364, 598
BsmAI GTCTC 2 cut(s) 26, 145
BsnI GGCC 2 cut(s) 69, 337
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 3 cut(s) 303, 343, 354
Bsp19I CCATGG 1 cut(s) 150
BspANI GGCC 2 cut(s) 69, 337
BspCNI CTCAG 1 cut(s) 591
BspPI GGATC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 40, 150
BssMI GATC 3 cut(s) 303, 343, 354
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 41, 365
BstACI GRCGYC 1 cut(s) 549
BstBAI YACGTR 1 cut(s) 535
BstC8I GCNNGC 2 cut(s) 67, 585
BstDEI CTNAG 3 cut(s) 6, 122, 578
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 373, 588
BstH2I RGCGCY 2 cut(s) 104, 238
BstHHI GCGC 2 cut(s) 103, 237
BstKTI GATC 3 cut(s) 306, 346, 357
BstMAI GTCTC 2 cut(s) 26, 145
BstMBI GATC 3 cut(s) 303, 343, 354
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 2 cut(s) 232, 334
BstNI CCWGG 2 cut(s) 41, 365
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 39, 363
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 289
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuI GTATCC 1 cut(s) 467
BsuRI GGCC 2 cut(s) 69, 337
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 373, 588
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 523
Cac8I GCNNGC 2 cut(s) 67, 585
CfoI GCGC 2 cut(s) 103, 237
CseI GACGC 1 cut(s) 557
Csp6I GTAC 3 cut(s) 12, 448, 532
CviAII CATG 4 cut(s) 151, 160, 184, 321
CviQI GTAC 3 cut(s) 12, 448, 532
DdeI CTNAG 3 cut(s) 6, 122, 578
DpnI GATC 3 cut(s) 305, 345, 356
DpnII GATC 3 cut(s) 303, 343, 354
EaeI YGGCCR 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 252
Eco47III AGCGCT 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 39, 363
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 4 cut(s) 154, 163, 187, 324
FaiI YATR 9 cut(s) 138, 152, 161, 185, 246, 322, 437, 443, 542
FatI CATG 4 cut(s) 150, 159, 183, 320
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 318
FokI GGATG 2 cut(s) 380, 575
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 318
FspBI CTAG 2 cut(s) 146, 465
GlaI GCGC 2 cut(s) 102, 236
GluI GCNGC 1 cut(s) 318
HaeII RGCGCY 2 cut(s) 104, 238
HaeIII GGCC 2 cut(s) 69, 337
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 103, 237
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 4 cut(s) 154, 163, 187, 324
Hin6I GCGC 2 cut(s) 101, 235
HinP1I GCGC 2 cut(s) 101, 235
HinfI GANTC 3 cut(s) 132, 371, 642
Hpy166II GTNNAC 2 cut(s) 181, 448
Hpy188I TCNGA 7 cut(s) 24, 343, 417, 458, 504, 560, 606
Hpy188III TCNNGA 4 cut(s) 217, 257, 301, 391
Hpy8I GTNNAC 2 cut(s) 181, 448
HpyAV CCTTC 1 cut(s) 491
HpyCH4IV ACGT 3 cut(s) 516, 534, 634
HpyCH4V TGCA 2 cut(s) 65, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 334
HpyF3I CTNAG 3 cut(s) 6, 122, 578
HpySE526I ACGT 3 cut(s) 516, 534, 634
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 4 cut(s) 154, 163, 187, 324
HspAI GCGC 2 cut(s) 101, 235
Kzo9I GATC 3 cut(s) 303, 343, 354
LmnI GCTCC 2 cut(s) 88, 314
Lsp1109I GCAGC 1 cut(s) 304
MaeI CTAG 2 cut(s) 146, 465
MaeII ACGT 3 cut(s) 516, 534, 634
MalI GATC 3 cut(s) 305, 345, 356
MboI GATC 3 cut(s) 303, 343, 354
MboII GAAGA 1 cut(s) 289
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 4 cut(s) 72, 283, 615, 649
MlyI GAGTC 1 cut(s) 141
MmeI TCCRAC 3 cut(s) 109, 583, 617
MnlI CCTC 7 cut(s) 126, 168, 253, 397, 411, 461, 464
MseI TTAA 2 cut(s) 519, 652
MspR9I CCNGG 2 cut(s) 41, 365
MvaI CCWGG 2 cut(s) 41, 365
MwoI GCNNNNNNNGC 2 cut(s) 232, 334
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 303, 343, 354
NlaIII CATG 4 cut(s) 154, 163, 187, 324
NspI RCATGY 1 cut(s) 324
PfeI GAWTC 2 cut(s) 371, 642
PflMI CCANNNNNTGG 1 cut(s) 364
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 1 cut(s) 319
Ple19I CGATCG 1 cut(s) 346
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Ppu21I YACGTR 1 cut(s) 535
Psp6I CCWGG 2 cut(s) 39, 363
PspGI CCWGG 2 cut(s) 39, 363
PsuI RGATCY 1 cut(s) 303
PvuI CGATCG 1 cut(s) 346
RsaI GTAC 3 cut(s) 13, 449, 533
RsaNI GTAC 3 cut(s) 12, 448, 532
SaqAI TTAA 2 cut(s) 519, 652
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 3 cut(s) 303, 343, 354
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 2 cut(s) 41, 365
SduI GDGCHC 1 cut(s) 252
SetI ASST 9 cut(s) 56, 93, 319, 422, 502, 515, 519, 537, 637
Sse9I AATT 4 cut(s) 72, 283, 615, 649
SspMI CTAG 2 cut(s) 146, 465
StyD4I CCNGG 2 cut(s) 39, 363
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 3 cut(s) 519, 537, 637
TasI AATT 4 cut(s) 72, 283, 615, 649
TfiI GAWTC 2 cut(s) 371, 642
Tru1I TTAA 2 cut(s) 519, 652
Tru9I TTAA 2 cut(s) 519, 652
TscAI CASTG 1 cut(s) 530
TseI GCWGC 1 cut(s) 317
TspRI CASTG 1 cut(s) 530
Van91I CCANNNNNTGG 1 cut(s) 364
XceI RCATGY 1 cut(s) 324
XcmI CCANNNNNNNNNTGG 2 cut(s) 289, 595
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 2 cut(s) 146, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.