RchiOBHm_Chr5g0055371

Glucomannan 4-beta-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0022545
Physical Location & Seq
Reverse (-)
58357799 .. 58359519
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33238

Sequence Viewer

Length: 798 bp
ATGGCTGAGAGTACCAAAGTTCTGATACCAGAGACATTCCAGGGTGGGAGTAGCTTTGACATTGCAGGCCAAATTGGGCTGATTTGGGAGCTAATCAAAGCGCCATTGATAGTTCCTCTGCTTAGAGTGGGAGTCTATGTCTCACTAGCCATGGTTCTCATGCTCTTTGTAGAGAGGGTCTACATGGGTATTGTCATTGTGTTGGTCAAAATGTTCTGGAAAAAGCCAGAGAAGCGCTACAACTATGAGCCCATTCAGGAGGATTTGGGATTGGGTAGTTCCAATTTCCCAATGGTGCTTGTTCAAATCCCCATGTTCAATGAAAGAGAGGTCTACAAGATCTCCATTGGAGCTGCATGTGGGCTGTCGTGGCCGTCCGATCGTCTTGTGATCCAAGTCCTAGATGATTCAACTGACCCTACAATCAAGAAAATGGTAGAGGCAGAGTGTCAGAGGTGGGCGAGAAAAGGCATAAACATAGTGTACCAAATCAGAGAAACTAGAGGAGGATACAAAGCAGGTGCATTGAAAGAAGGTTTGAAACGAAGCTACGTGAAACACTGCGAGTACGTGGCTATAATGGACGCCGATTTCCGACCCGAGCCAGACTTTCTCAGGCGAGCCATCCCTTTCTTGGTTCAGAACCCCGAAATTGCTTTGGTTCAAGCACGTTGGAGATTCGCTAAGGATGATACGCCACTAATGAAGGTAGTTTATCGGATTTACAAAAGGTGGACAGTCATGGAAGTGATGGAACCCATTAGCTTTAGTTTATGCAGAGTTTGCATAATTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3 Glycos_transf_2

Protein Analysis

265

Amino Acids

30.45

Weight (kDa)

8.99

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 97 - 248 5.3e-06 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 102 - 233 2.6e-11 Glycosyl transferase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022545)

Species Orthologous Gene IDs
pyrus_communis pycom07g05490
rosa_chinensis RchiOBHm_Chr5g0055371
rosa_multiflora Rmu_sc0001673.1_g000013 Rmu_sc0018900.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 509
AccI GTMKAC 2 cut(s) 180, 333
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 3 cut(s) 13, 485, 569
AfeI AGCGCT 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 634
AgsI TTSAA 6 cut(s) 305, 319, 411, 529, 541, 665
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 5 cut(s) 54, 91, 353, 549, 765
AluI AGCT 5 cut(s) 54, 91, 353, 549, 765
Alw26I GTCTC 2 cut(s) 26, 145
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 599
Aor51HI AGCGCT 1 cut(s) 236
AoxI GGCC 2 cut(s) 67, 371
ApeKI GCWGC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 103, 237
AvaI CYCGRG 1 cut(s) 599
BanII GRGCYC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 632, 745
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 1 cut(s) 41
BciVI GTATCC 1 cut(s) 503
BcoDI GTCTC 2 cut(s) 26, 145
BfaI CTAG 3 cut(s) 146, 401, 501
BfoI RGCGCY 2 cut(s) 104, 238
BfuAI ACCTGC 1 cut(s) 509
BfuI GTATCC 1 cut(s) 503
BglII AGATCT 1 cut(s) 339
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme1390I CCNGG 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 599
BmiI GGNNCC 1 cut(s) 756
BmrFI CCNGG 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 684
BsaAI YACGTR 2 cut(s) 553, 571
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 40, 150
BsaXI ACNNNNNCTCC 4 cut(s) 123, 153, 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 40, 150
BseGI GGATG 2 cut(s) 624, 694
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 628
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 382
BshFI GGCC 2 cut(s) 69, 373
BsiEI CGRYCG 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 599
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 2 cut(s) 26, 145
BsnI GGCC 2 cut(s) 69, 373
BsoBI CYCGRG 1 cut(s) 599
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 3 cut(s) 339, 379, 390
Bsp19I CCATGG 1 cut(s) 150
BspANI GGCC 2 cut(s) 69, 373
BspCNI CTCAG 1 cut(s) 627
BspLI GGNNCC 1 cut(s) 756
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 1 cut(s) 385
BsrDI GCAATG 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 40, 150
BssMI GATC 3 cut(s) 339, 379, 390
BssNI GRCGYC 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 739
BstACI GRCGYC 1 cut(s) 585
BstAPI GCANNNNNTGC 1 cut(s) 783
BstBAI YACGTR 2 cut(s) 553, 571
BstC8I GCNNGC 2 cut(s) 67, 621
BstDEI CTNAG 4 cut(s) 6, 122, 614, 684
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 624, 694
BstH2I RGCGCY 2 cut(s) 104, 238
BstHHI GCGC 2 cut(s) 103, 237
BstKTI GATC 3 cut(s) 342, 382, 393
BstMAI GTCTC 2 cut(s) 26, 145
BstMBI GATC 3 cut(s) 339, 379, 390
BstMCI CGRYCG 1 cut(s) 382
BstMWI GCNNNNNNNGC 3 cut(s) 232, 370, 783
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
BsuI GTATCC 1 cut(s) 503
BsuRI GGCC 2 cut(s) 69, 373
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 624, 694
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 1 cut(s) 559
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 2 cut(s) 67, 621
CfoI GCGC 2 cut(s) 103, 237
CseI GACGC 1 cut(s) 593
Csp6I GTAC 3 cut(s) 12, 484, 568
CviAII CATG 6 cut(s) 151, 160, 184, 313, 357, 742
CviQI GTAC 3 cut(s) 12, 484, 568
DdeI CTNAG 4 cut(s) 6, 122, 614, 684
DpnI GATC 3 cut(s) 341, 381, 392
DpnII GATC 3 cut(s) 339, 379, 390
EaeI YGGCCR 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 252
Eco47III AGCGCT 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 599
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 6 cut(s) 154, 163, 187, 316, 360, 745
FatI CATG 6 cut(s) 150, 159, 183, 312, 356, 741
FblI GTMKAC 2 cut(s) 180, 333
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 2 cut(s) 611, 701
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 354
FspBI CTAG 3 cut(s) 146, 401, 501
GlaI GCGC 2 cut(s) 102, 236
GluI GCNGC 1 cut(s) 354
HaeII RGCGCY 2 cut(s) 104, 238
HaeIII GGCC 2 cut(s) 69, 373
HgaI GACGC 1 cut(s) 593
HhaI GCGC 2 cut(s) 103, 237
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 6 cut(s) 154, 163, 187, 316, 360, 745
Hin6I GCGC 2 cut(s) 101, 235
HinP1I GCGC 2 cut(s) 101, 235
HinfI GANTC 3 cut(s) 132, 407, 678
Hpy166II GTNNAC 4 cut(s) 181, 334, 484, 735
Hpy188I TCNGA 7 cut(s) 24, 379, 453, 494, 596, 642, 720
Hpy188III TCNNGA 3 cut(s) 217, 257, 427
Hpy8I GTNNAC 4 cut(s) 181, 334, 484, 735
HpyAV CCTTC 2 cut(s) 527, 700
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4IV ACGT 3 cut(s) 552, 570, 670
HpyCH4V TGCA 5 cut(s) 65, 356, 524, 777, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 232, 370, 783
HpyF3I CTNAG 4 cut(s) 6, 122, 614, 684
HpySE526I ACGT 3 cut(s) 552, 570, 670
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 6 cut(s) 154, 163, 187, 316, 360, 745
HspAI GCGC 2 cut(s) 101, 235
Kzo9I GATC 3 cut(s) 339, 379, 390
LmnI GCTCC 2 cut(s) 88, 350
Lsp1109I GCAGC 1 cut(s) 340
MaeI CTAG 3 cut(s) 146, 401, 501
MaeII ACGT 3 cut(s) 552, 570, 670
MalI GATC 3 cut(s) 341, 381, 392
MboI GATC 3 cut(s) 339, 379, 390
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 4 cut(s) 72, 283, 651, 789
MlyI GAGTC 1 cut(s) 141
MmeI TCCRAC 2 cut(s) 619, 653
MnlI CCTC 8 cut(s) 126, 168, 253, 322, 433, 447, 497, 500
MslI CAYNNNNRTG 1 cut(s) 746
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 3 cut(s) 232, 370, 783
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 339, 379, 390
NlaIII CATG 6 cut(s) 154, 163, 187, 316, 360, 745
NlaIV GGNNCC 1 cut(s) 756
NspI RCATGY 1 cut(s) 360
PaqCI CACCTGC 1 cut(s) 509
PfeI GAWTC 2 cut(s) 407, 678
PkrI GCNGC 1 cut(s) 355
Ple19I CGATCG 1 cut(s) 382
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Ppu21I YACGTR 2 cut(s) 553, 571
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 756
PsuI RGATCY 1 cut(s) 339
PvuI CGATCG 1 cut(s) 382
RsaI GTAC 3 cut(s) 13, 485, 569
RsaNI GTAC 3 cut(s) 12, 484, 568
RseI CAYNNNNRTG 1 cut(s) 746
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 3 cut(s) 339, 379, 390
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 746
Sse9I AATT 4 cut(s) 72, 283, 651, 789
SspMI CTAG 3 cut(s) 146, 401, 501
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 739
TaiI ACGT 3 cut(s) 555, 573, 673
TasI AATT 4 cut(s) 72, 283, 651, 789
TfiI GAWTC 2 cut(s) 407, 678
TscAI CASTG 1 cut(s) 566
TseI GCWGC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 336, 719, 783
TspRI CASTG 1 cut(s) 566
XceI RCATGY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 2 cut(s) 289, 631
XmiI GTMKAC 2 cut(s) 180, 333
XspI CTAG 3 cut(s) 146, 401, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.