pycom07g07160

3-isopropylmalate dehydrogenase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0024474
Physical Location & Seq
Reverse (-)
6497744 .. 6498887
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g07160.2

Sequence Viewer

Length: 786 bp
ATGCATGGAAATTTCTTCCATGGGACGATTCCATCAACTTTGAGTTCACTAAGAGTTCTTACAGAATTGTATCTTTATGACAACTTGTCAGGAGCAATTCCAGAATTCTTAGAACATTTGAATTTTTTGGAATCTCTGAATCTATCTTATAACAATTTTGAGGGCATGTTGCCGATCAAGGGAGTGTTCAAGAATGCACTGGTGACATCAGTTGAAGGAAATAGCAAGCTTTCGGGGGGCATAACTGAGTTTCATTTGCCAAACACGGCTTACAAAAGTTTTATTTATGAGTGCGAGGCTTTCCGAAATATTAGACACCGTAATCTTGTCAAAATAGTCTTAGTATGTTCTGGTTTTGATTATCGCGGTCACGACTTCAAGGCCTTAATTTACGAGTTCATGGCTAACGGGAGCTTGGAGGAATGGCTATATCCAATTCATACAATTGGTGAGACAAATGAGAGGCCAAGGACCTTAACAATTTCTCAGAGTTTGAATATTGTTTTTGATGTTGCTATGGCATTGGATTATCTCCATCGTCATTGTGAGATGCCAGTACTTCATTGCAACCTCAAACCTAGCAATATTCTTCTCAATGAGGATATGATTGCACATGTAGGTGACTTTGGGTTGGTGAGGTTCCTACCAAAAGTTGCTGAAACACTACGAAAAATATGGGCTTTGGTGTACACAAATGAAGAGTTTGTGTCTTTGAGAACACTAATATGGTGGAACAGTGTCCATTCAATAAAGGATACGATGTATTGTGCCCACCATGCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00660 ko01100 ko01110 ko01210 ko01230 map00290 map00660 map01100 map01110 map01210 map01230
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

262

Amino Acids

30.0

Weight (kDa)

6.26

Isoelectric Point (pI)

28.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024474)

Species Orthologous Gene IDs
malus_domestica MD07G1089500.v1.1
pyrus_communis pycom02g18010 pycom07g07160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AccII CGCG 1 cut(s) 366
AciI CCGC 1 cut(s) 366
AcsI RAATTY 3 cut(s) 10, 104, 121
AfaI GTAC 2 cut(s) 558, 689
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 613
AgsI TTSAA 6 cut(s) 121, 190, 215, 379, 496, 747
AhdI GACNNNNNGTC 1 cut(s) 85
AluBI AGCT 2 cut(s) 229, 414
AluI AGCT 2 cut(s) 229, 414
Alw26I GTCTC 1 cut(s) 446
AoxI GGCC 2 cut(s) 381, 464
ApoI RAATTY 3 cut(s) 10, 104, 121
AspS9I GGNCC 1 cut(s) 471
AsuHPI GGTGA 4 cut(s) 214, 461, 632, 646
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 772
BccI CCATC 2 cut(s) 40, 543
BceAI ACGGC 1 cut(s) 282
BciVI GTATCC 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 1 cut(s) 579
BfuI GTATCC 1 cut(s) 748
BmcAI AGTACT 1 cut(s) 558
Bme18I GGWCC 1 cut(s) 471
BmeRI GACNNNNNGTC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 471
BmiI GGNNCC 1 cut(s) 641
BmsI GCATC 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 19, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 2 cut(s) 204, 554
Bse3DI GCAATG 1 cut(s) 562
BseDI CCNNGG 2 cut(s) 19, 467
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 2 cut(s) 237, 500
BseNI ACTGG 2 cut(s) 204, 554
BseSI GKGCMC 1 cut(s) 772
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 383, 466
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 2 cut(s) 383, 466
Bsp1286I GDGCHC 1 cut(s) 772
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 1 cut(s) 174
Bsp19I CCATGG 1 cut(s) 19
BspACI CCGC 1 cut(s) 366
BspANI GGCC 2 cut(s) 383, 466
BspCNI CTCAG 2 cut(s) 238, 499
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 562
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 204, 554
BssECI CCNNGG 2 cut(s) 19, 467
BssMI GATC 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 19, 467
Bst4CI ACNGT 2 cut(s) 320, 737
Bst6I CTCTTC 1 cut(s) 693
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 5 cut(s) 50, 109, 246, 340, 486
BstDSI CCRYGG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 366
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 776
BstNSI RCATGY 2 cut(s) 169, 617
BstSLI GKGCMC 1 cut(s) 772
BstUI CGCG 1 cut(s) 366
BsuI GTATCC 1 cut(s) 748
BsuRI GGCC 2 cut(s) 383, 466
BtgI CCRYGG 1 cut(s) 19
BtsIMutI CAGTG 2 cut(s) 197, 742
Cac8I GCNNGC 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 471
Csp6I GTAC 2 cut(s) 557, 688
CviAII CATG 6 cut(s) 5, 20, 166, 400, 614, 776
CviJI RGCY 9 cut(s) 229, 269, 299, 383, 404, 414, 427, 466, 680
CviKI_1 RGCY 9 cut(s) 229, 269, 299, 383, 404, 414, 427, 466, 680
CviQI GTAC 2 cut(s) 557, 688
DdeI CTNAG 5 cut(s) 50, 109, 246, 340, 486
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 693
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 693
Eco130I CCWWGG 2 cut(s) 19, 467
Eco147I AGGCCT 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 104
EcoT14I CCWWGG 2 cut(s) 19, 467
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 19, 467
FaeI CATG 6 cut(s) 8, 23, 169, 403, 617, 779
FaqI GGGAC 1 cut(s) 37
FatI CATG 6 cut(s) 4, 19, 165, 399, 613, 775
FspBI CTAG 1 cut(s) 579
HaeIII GGCC 2 cut(s) 383, 466
Hin1II CATG 6 cut(s) 8, 23, 169, 403, 617, 779
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 3 cut(s) 28, 131, 139
HphI GGTGA 4 cut(s) 214, 461, 632, 646
Hpy166II GTNNAC 3 cut(s) 47, 688, 690
Hpy188I TCNGA 3 cut(s) 138, 305, 489
Hpy188III TCNNGA 4 cut(s) 90, 101, 190, 371
Hpy8I GTNNAC 3 cut(s) 47, 688, 690
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 320, 737
HpyCH4V TGCA 4 cut(s) 4, 197, 567, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 776
HpyF3I CTNAG 5 cut(s) 50, 109, 246, 340, 486
Hsp92II CATG 6 cut(s) 8, 23, 169, 403, 617, 779
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 92, 411
LpnPI CCDG 5 cut(s) 75, 114, 185, 336, 567
LweI GCATC 1 cut(s) 540
MaeI CTAG 1 cut(s) 579
MaeIII GTNAC 3 cut(s) 202, 368, 620
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 3 cut(s) 7, 581, 710
MfeI CAATTG 1 cut(s) 444
MhlI GDGCHC 1 cut(s) 772
MnlI CCTC 7 cut(s) 154, 289, 412, 456, 581, 592, 630
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 386, 476
MslI CAYNNNNRTG 2 cut(s) 618, 724
MunI CAATTG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 199
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 776
NcoI CCATGG 1 cut(s) 19
NdeII GATC 1 cut(s) 174
NlaIII CATG 6 cut(s) 8, 23, 169, 403, 617, 779
NlaIV GGNNCC 1 cut(s) 641
NmuCI GTSAC 3 cut(s) 202, 368, 620
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 169, 617
PceI AGGCCT 1 cut(s) 383
PciI ACATGT 1 cut(s) 613
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 28, 131, 139
PpuMI RGGWCCY 1 cut(s) 471
PscI ACATGT 1 cut(s) 613
PsiI TTATAA 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 641
PspPI GGNCC 1 cut(s) 471
PspPPI RGGWCCY 1 cut(s) 471
RsaI GTAC 2 cut(s) 558, 689
RsaNI GTAC 2 cut(s) 557, 688
RseI CAYNNNNRTG 2 cut(s) 618, 724
SaqAI TTAA 2 cut(s) 386, 476
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 471
ScaI AGTACT 1 cut(s) 558
SduI GDGCHC 1 cut(s) 772
SetI ASST 7 cut(s) 231, 416, 476, 573, 580, 622, 641
SfaNI GCATC 1 cut(s) 540
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 2 cut(s) 618, 724
SseBI AGGCCT 1 cut(s) 383
SsiI CCGC 1 cut(s) 366
SspI AATATT 3 cut(s) 310, 499, 586
SspMI CTAG 1 cut(s) 579
StuI AGGCCT 1 cut(s) 383
StyI CCWWGG 2 cut(s) 19, 467
TaaI ACNGT 2 cut(s) 320, 737
TatI WGTACW 2 cut(s) 556, 687
TfiI GAWTC 3 cut(s) 28, 131, 139
Tru1I TTAA 2 cut(s) 386, 476
Tru9I TTAA 2 cut(s) 386, 476
TscAI CASTG 2 cut(s) 204, 742
TseFI GTSAC 3 cut(s) 202, 368, 620
Tsp45I GTSAC 3 cut(s) 202, 368, 620
TspDTI ATGAA 5 cut(s) 242, 388, 428, 551, 711
TspRI CASTG 2 cut(s) 204, 742
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 3 cut(s) 10, 104, 121
XceI RCATGY 2 cut(s) 169, 617
XspI CTAG 1 cut(s) 579
ZrmI AGTACT 1 cut(s) 558
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.