pycom07g10350

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0021269
Physical Location & Seq
Reverse (-)
10237177 .. 10238093
917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGAAATTTTTTACATCCGAAAGGGAAAATTTGAATCTAAACTAGAGATCATTGAAAATCTTAGTAAAGGTGAATTACCAAACAACGAGTATTCATGCTTGAATGAACCTACTCCGGCTCCCCAAGGGGGCAGTGTGAGAGGCCACAGTTCATCGGCGCAGACAAGCCAATCTACCAGTGCTCCTCATTCAATGAGATCAAGGCGGACTGCTAACTGGGGACGGGCTCGGCATTCTGATGATGGATATTCAAGTGACTCAGTTTTGAGAGGTTCATCTACTGATTTCAAGAAGATGGGGCAACGTATCTTTGTATTTATGATTGGTGGGGCAACTCGATCTGAGTTACGAGTTTGTCACAAGCTGACGACGAAATTGAGAAGGGAAGTAATCCTGGGTACTACTGGTCTCATTGAACCCCCACAGTATATTACGAAACTGAAGCTGCTATCAGAAAAAGAGCTAGCAATGGATGGCCTTGGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 map04721
Pfam Domains
Protein Families
Sec1

Protein Analysis

163

Amino Acids

17.99

Weight (kDa)

9.61

Isoelectric Point (pI)

61.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 16 - 145 1.9e-16 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0021269)

Species Orthologous Gene IDs
pyrus_communis pycom07g10350
rosa_multiflora Rmu_co8350817.1_g000001
rosa_samantha Rh5BG264300 Rh5CG297000 Rh5DG272700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 205
AcsI RAATTY 2 cut(s) 6, 29
AcuI CTGAAG 1 cut(s) 461
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 7 cut(s) 35, 56, 103, 192, 252, 289, 416
AjnI CCWGG 1 cut(s) 393
AluBI AGCT 3 cut(s) 364, 445, 463
AluI AGCT 3 cut(s) 364, 445, 463
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 413
AoxI GGCC 2 cut(s) 142, 475
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 2 cut(s) 6, 29
AspLEI GCGC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 83
AsuNHI GCTAGC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 432
BccI CCATC 3 cut(s) 236, 289, 467
BciT130I CCWGG 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 2 cut(s) 44, 464
BisI GCNGC 1 cut(s) 446
BlsI GCNGC 1 cut(s) 447
Bme1390I CCNGG 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 395
BmrI ACTGGG 1 cut(s) 226
BmtI GCTAGC 1 cut(s) 467
BmuI ACTGGG 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 3 cut(s) 124, 394, 478
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 166, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 3 cut(s) 177, 221, 409
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 3 cut(s) 124, 394, 478
BseGI GGATG 2 cut(s) 15, 478
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 273, 333
BseNI ACTGG 3 cut(s) 177, 221, 409
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 432
BshFI GGCC 2 cut(s) 144, 477
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 2 cut(s) 144, 477
Bso31I GGTCTC 1 cut(s) 413
Bsp1286I GDGCHC 2 cut(s) 184, 229
Bsp143I GATC 3 cut(s) 48, 197, 338
BspACI CCGC 1 cut(s) 205
BspANI GGCC 2 cut(s) 144, 477
BspCNI CTCAG 2 cut(s) 272, 334
BspLI GGNNCC 1 cut(s) 120
BspOI GCTAGC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 3 cut(s) 177, 221, 409
BssECI CCNNGG 3 cut(s) 124, 394, 478
BssMI GATC 3 cut(s) 48, 197, 338
BssT1I CCWWGG 2 cut(s) 124, 478
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 2 cut(s) 149, 426
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 3 cut(s) 62, 259, 342
BstF5I GGATG 2 cut(s) 15, 478
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 51, 200, 341
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 3 cut(s) 48, 197, 338
BstNI CCWGG 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 432
BsuRI GGCC 2 cut(s) 144, 477
BtsCI GGATG 2 cut(s) 15, 478
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 2 cut(s) 139, 184
Cac8I GCNNGC 1 cut(s) 465
CfoI GCGC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 399
CviAII CATG 1 cut(s) 96
CviJI RGCY 8 cut(s) 119, 144, 168, 227, 364, 445, 463, 477
CviKI_1 RGCY 8 cut(s) 119, 144, 168, 227, 364, 445, 463, 477
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 3 cut(s) 62, 259, 342
DpnI GATC 3 cut(s) 50, 199, 340
DpnII GATC 3 cut(s) 48, 197, 338
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 2 cut(s) 124, 478
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 1 cut(s) 413
Eco57I CTGAAG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 2 cut(s) 124, 478
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 124, 478
FaeI CATG 1 cut(s) 99
FaiI YATR 3 cut(s) 97, 320, 429
FaqI GGGAC 1 cut(s) 234
FatI CATG 1 cut(s) 95
Fnu4HI GCNGC 1 cut(s) 446
FokI GGATG 2 cut(s) 2, 485
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 446
FspBI CTAG 2 cut(s) 44, 464
GlaI GCGC 1 cut(s) 159
GluI GCNGC 1 cut(s) 446
HaeIII GGCC 2 cut(s) 144, 477
HapII CCGG 1 cut(s) 116
HhaI GCGC 1 cut(s) 160
Hin1II CATG 1 cut(s) 99
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 3 cut(s) 35, 257, 483
HpaII CCGG 1 cut(s) 116
HphI GGTGA 1 cut(s) 83
Hpy188I TCNGA 4 cut(s) 20, 238, 343, 454
Hpy188III TCNNGA 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 373
HpyAV CCTTC 1 cut(s) 375
HpyCH4III ACNGT 2 cut(s) 149, 426
HpyCH4IV ACGT 1 cut(s) 304
HpyF3I CTNAG 3 cut(s) 62, 259, 342
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 1 cut(s) 99
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 48, 197, 338
LmnI GCTCC 2 cut(s) 124, 187
LpnPI CCDG 6 cut(s) 129, 190, 202, 380, 390, 407
Lsp1109I GCAGC 1 cut(s) 432
MaeI CTAG 2 cut(s) 44, 464
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 3 cut(s) 254, 345, 356
MalI GATC 3 cut(s) 50, 199, 340
MboI GATC 3 cut(s) 48, 197, 338
MboII GAAGA 1 cut(s) 304
MhlI GDGCHC 2 cut(s) 184, 229
MluCI AATT 4 cut(s) 6, 29, 74, 374
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 3 cut(s) 134, 195, 263
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 395
Mva1269I GAATGC 1 cut(s) 232
MvaI CCWGG 1 cut(s) 395
NdeII GATC 3 cut(s) 48, 197, 338
NheI GCTAGC 1 cut(s) 463
NlaIII CATG 1 cut(s) 99
NlaIV GGNNCC 1 cut(s) 120
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 254, 356
PctI GAATGC 1 cut(s) 232
PfeI GAWTC 2 cut(s) 35, 483
PkrI GCNGC 1 cut(s) 447
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 1 cut(s) 120
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
RseI CAYNNNNRTG 1 cut(s) 237
SatI GCNGC 1 cut(s) 446
Sau3AI GATC 3 cut(s) 48, 197, 338
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 2 cut(s) 184, 229
SetI ASST 7 cut(s) 73, 112, 274, 307, 366, 447, 465
SmiMI CAYNNNNRTG 1 cut(s) 237
Sse9I AATT 4 cut(s) 6, 29, 74, 374
SsiI CCGC 1 cut(s) 205
SspMI CTAG 2 cut(s) 44, 464
StyD4I CCNGG 1 cut(s) 393
StyI CCWWGG 2 cut(s) 124, 478
TaaI ACNGT 2 cut(s) 149, 426
TaiI ACGT 1 cut(s) 307
TaqI TCGA 1 cut(s) 337
TasI AATT 4 cut(s) 6, 29, 74, 374
TfiI GAWTC 2 cut(s) 35, 483
TscAI CASTG 2 cut(s) 139, 184
TseFI GTSAC 2 cut(s) 254, 356
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 2 cut(s) 254, 356
TspDTI ATGAA 4 cut(s) 84, 120, 141, 264
TspRI CASTG 2 cut(s) 139, 184
XapI RAATTY 2 cut(s) 6, 29
XspI CTAG 2 cut(s) 44, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.