Rh5DG272700

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0021269
Physical Location & Seq
Reverse (-)
33253074 .. 33255024
1951 bp
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UTR
Exon/CDS
Intron
Rh5DG272700.1

Sequence Viewer

Length: 612 bp
ATGATTCTTGCTGCCATCTATCCTGAGAAATTTGAGGATGAAGAAGATCTACATATGATGATGAAGTACGCATATTTACTACCCCAGGATATGACTGTTGTGAGGAATATGAAGTTGATTGGGGAGAGCAGAGAAGCCTCATCTGGAATTTTTCCTCTGAAGTTTGGTATTGCTAAGAGAAAATGTTATAGGAAAGAGCGTCCAAGAGAAGATGAATGGCTTTATTCGCGCTTTTTTCCGATGATAGAGGAACTTATAGAAAAGCTTAGCATTGGTACACTGTCAGAGGAATTATATCCATGTGTGAATCCCAACGCTCGTTTACTGAGATCGAGGAGGACACCAATGTGGGCCCACCGCCGGAATTCCGATAATGGGTTTTCAAGGGATGCATCTACAGATTTCGAAAAGATGGGCCGACGCATATTTGTAGTTATAGTAGGTGGAGCTACTAGATCAGAGCTTAGGGTTTGTCGGAAACTCACAGCAAAGCTCAAAAGGGAAGTTATTCTGGGCTCCTCATCTCTTGACGATCCTGCAACTTTTGTTACGAATCTGAAAATGATCACAGCAGACGACCAACCGCTCACGTTGGATGATATCAAGATCTGA

Protein Analysis

203

Amino Acids

23.56

Weight (kDa)

8.94

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 21 - 184 1.7e-18 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021269)

Species Orthologous Gene IDs
pyrus_communis pycom07g10350
rosa_multiflora Rmu_co8350817.1_g000001
rosa_samantha Rh5BG264300 Rh5CG297000 Rh5DG272700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 586
AccII CGCG 1 cut(s) 229
AciI CCGC 2 cut(s) 358, 584
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 3 cut(s) 29, 147, 364
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 2 cut(s) 68, 277
AfiI CCNNNNNNNGG 2 cut(s) 360, 375
AgsI TTSAA 1 cut(s) 384
AjnI CCWGG 1 cut(s) 84
AleI CACNNNNGTG 1 cut(s) 346
AluBI AGCT 4 cut(s) 265, 449, 463, 493
AluI AGCT 4 cut(s) 265, 449, 463, 493
AlwI GGATC 1 cut(s) 527
AoxI GGCC 2 cut(s) 351, 415
ApaI GGGCCC 1 cut(s) 355
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 3 cut(s) 29, 147, 364
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 1 cut(s) 231
AspS9I GGNCC 3 cut(s) 351, 352, 415
AsuII TTCGAA 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 355, 518
BarI GAAGNNNNNNTAC 2 cut(s) 33, 65
BccI CCATC 2 cut(s) 23, 406
BciT130I CCWGG 1 cut(s) 86
BclI TGATCA 1 cut(s) 564
BfaI CTAG 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 396
BglII AGATCT 2 cut(s) 46, 606
BisI GCNGC 1 cut(s) 12
BlpI GCTNAGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 86
BmgT120I GGNCC 3 cut(s) 351, 352, 415
BmiI GGNNCC 2 cut(s) 353, 517
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 2 cut(s) 379, 401
Bpu10I CCTNAGC 1 cut(s) 464
Bpu1102I GCTNAGC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 84
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 375
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 3 cut(s) 43, 394, 601
BseLI CCNNNNNNNGG 2 cut(s) 360, 375
BseMII CTCAG 2 cut(s) 15, 317
BseRI GAGGAG 2 cut(s) 349, 508
BseSI GKGCMC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 2 cut(s) 353, 417
BsiSI CCGG 1 cut(s) 361
BslI CCNNNNNNNGG 2 cut(s) 360, 375
BsnI GGCC 2 cut(s) 353, 417
Bsp119I TTCGAA 1 cut(s) 405
Bsp120I GGGCCC 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 355, 518
Bsp143I GATC 6 cut(s) 46, 329, 455, 532, 564, 606
Bsp1720I GCTNAGC 1 cut(s) 266
BspACI CCGC 2 cut(s) 358, 584
BspANI GGCC 2 cut(s) 353, 417
BspCNI CTCAG 2 cut(s) 16, 318
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 353, 517
BspPI GGATC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 405
BsrBI CCGCTC 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 6 cut(s) 46, 329, 455, 532, 564, 606
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 2 cut(s) 97, 282
BstBI TTCGAA 1 cut(s) 405
BstDEI CTNAG 5 cut(s) 24, 174, 266, 326, 464
BstF5I GGATG 3 cut(s) 43, 394, 601
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 6 cut(s) 49, 332, 458, 535, 567, 609
BstMBI GATC 6 cut(s) 46, 329, 455, 532, 564, 606
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 355
BstUI CGCG 1 cut(s) 229
BstX2I RGATCY 2 cut(s) 46, 606
BstYI RGATCY 2 cut(s) 46, 606
BsuRI GGCC 2 cut(s) 353, 417
BtsCI GGATG 3 cut(s) 43, 394, 601
BtsIMutI CAGTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 231
Cfr13I GGNCC 3 cut(s) 351, 352, 415
CseI GACGC 2 cut(s) 188, 429
Csp6I GTAC 2 cut(s) 67, 276
CviAII CATG 1 cut(s) 300
CviJI RGCY 9 cut(s) 137, 220, 265, 353, 417, 449, 463, 493, 516
CviKI_1 RGCY 9 cut(s) 137, 220, 265, 353, 417, 449, 463, 493, 516
CviQI GTAC 2 cut(s) 67, 276
DdeI CTNAG 5 cut(s) 24, 174, 266, 326, 464
DpnI GATC 6 cut(s) 48, 331, 457, 534, 566, 608
DpnII GATC 6 cut(s) 46, 329, 455, 532, 564, 606
Eco24I GRGCYC 2 cut(s) 355, 518
Eco32I GATATC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 84
EcoRV GATATC 1 cut(s) 601
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 2 cut(s) 355, 518
FaeI CATG 1 cut(s) 303
FatI CATG 1 cut(s) 299
FauNDI CATATG 1 cut(s) 54
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 3 cut(s) 50, 401, 608
FriOI GRGCYC 2 cut(s) 355, 518
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 453
GlaI GCGC 1 cut(s) 230
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 2 cut(s) 353, 417
HapII CCGG 1 cut(s) 361
HgaI GACGC 2 cut(s) 188, 429
HhaI GCGC 1 cut(s) 231
Hin1II CATG 1 cut(s) 303
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 3 cut(s) 4, 307, 553
HpaII CCGG 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 278, 323
Hpy188I TCNGA 8 cut(s) 159, 240, 286, 370, 460, 477, 558, 611
Hpy188III TCNNGA 4 cut(s) 23, 144, 527, 604
Hpy8I GTNNAC 2 cut(s) 278, 323
Hpy99I CGWCG 1 cut(s) 423
HpyCH4III ACNGT 2 cut(s) 97, 282
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 2 cut(s) 392, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 5 cut(s) 24, 174, 266, 326, 464
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 1 cut(s) 303
HspAI GCGC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 6 cut(s) 46, 329, 455, 532, 564, 606
LmnI GCTCC 2 cut(s) 446, 521
LpnPI CCDG 7 cut(s) 36, 71, 98, 129, 374, 497, 549
LweI GCATC 2 cut(s) 379, 401
MaeI CTAG 1 cut(s) 453
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 1 cut(s) 547
MalI GATC 6 cut(s) 48, 331, 457, 534, 566, 608
MbiI CCGCTC 1 cut(s) 586
MboI GATC 6 cut(s) 46, 329, 455, 532, 564, 606
MboII GAAGA 3 cut(s) 53, 56, 221
MflI RGATCY 2 cut(s) 46, 606
MhlI GDGCHC 2 cut(s) 355, 518
MluCI AATT 4 cut(s) 29, 147, 290, 364
MmeI TCCRAC 2 cut(s) 455, 573
MnlI CCTC 9 cut(s) 28, 96, 148, 165, 241, 280, 327, 330, 529
Mph1103I ATGCAT 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 346
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeI CATATG 1 cut(s) 54
NdeII GATC 6 cut(s) 46, 329, 455, 532, 564, 606
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 2 cut(s) 353, 517
NsiI ATGCAT 1 cut(s) 394
NspV TTCGAA 1 cut(s) 405
OliI CACNNNNGTG 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 3 cut(s) 4, 307, 553
PkrI GCNGC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 353, 517
PspOMI GGGCCC 1 cut(s) 351
PspPI GGNCC 3 cut(s) 351, 352, 415
PsuI RGATCY 2 cut(s) 46, 606
RsaI GTAC 2 cut(s) 68, 277
RsaNI GTAC 2 cut(s) 67, 276
RseI CAYNNNNRTG 1 cut(s) 346
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 6 cut(s) 46, 329, 455, 532, 564, 606
Sau96I GGNCC 3 cut(s) 351, 352, 415
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 2 cut(s) 355, 518
SetI ASST 6 cut(s) 267, 445, 451, 465, 495, 593
SfaNI GCATC 2 cut(s) 379, 401
SfcI CTRYAG 1 cut(s) 396
SfuI TTCGAA 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 346
Sse9I AATT 4 cut(s) 29, 147, 290, 364
SsiI CCGC 2 cut(s) 358, 584
SspMI CTAG 1 cut(s) 453
StyD4I CCNGG 1 cut(s) 84
TaaI ACNGT 2 cut(s) 97, 282
TaiI ACGT 1 cut(s) 593
TaqI TCGA 2 cut(s) 332, 405
TasI AATT 4 cut(s) 29, 147, 290, 364
TfiI GAWTC 3 cut(s) 4, 307, 553
TscAI CASTG 1 cut(s) 285
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 4 cut(s) 54, 77, 125, 228
TspRI CASTG 1 cut(s) 285
XapI RAATTY 3 cut(s) 29, 147, 364
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 1 cut(s) 453
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.