pycom07g14440

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0020338
Physical Location & Seq
Reverse (-)
16391098 .. 16391880
783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGAGTTCTTTTGATAGATACTTTTTCTCTACAAGAAAAAACGAGAAGAAATATTGTGATGATTATTCTCGACTATTACTCAAGTGGATCCATAATGCAGTAAGATGTAATGTTGAAGATCTTGATCTCGATTTCGATCACTGTGTTATTAGTAAGTTTTCGTTGCCATCTTTCATCTTGCATTCTCAATCTTTGAGCTCTCTATCGGTCTCCTTGGGTATAATCAATTCAGACGTGGTTGAGGGGCCATCTCTATTCTTTTCTAGTAATCTCGGTTACCTATCGTTGTGTTATGTTAAAATAGTAGATGAGAGTCTTTTCAAATGGATCTCATGCTGCTGCAAATGCCTCAAGGAGTTACATCTTAGATCTATCAAAGGCATACAAGATATCTCCATCGAAAGTTCGTCTTTGGAATCCTTTTCTTTTATCAATCATGGTGACATGCTATGGAATGGTTGGTTTGTACCAACAAGCATGAAAAGTAGCACATCGTTGAAGATTTTTGCTCCAAATCTTAAAAATCTGAAATGGGACGGCCCATTAAGTAGCCAAAATCTGGGAAAATTTGAGAGTTTGGAAAAAGTTGAGATTTGTTTAGAGCCTCGGAAAAATGAGCTTGGCAAAATACTTGAGGTTCCTCGCAGTATATGCTCGCAAAAGTTCTTATTATACACACGAAGACCGCTAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

232

Amino Acids

26.56

Weight (kDa)

8.48

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 19 - 145 4.7e-08 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 28 - 145 5.4e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020338)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18950
malus_domestica MD04G1145800.v1.1
pyrus_communis pycom07g14440
rosa_samantha Rh3AG187400 Rh3BG215900 Rh3DG212000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 559
AciI CCGC 1 cut(s) 686
AclWI GGATC 3 cut(s) 82, 95, 335
AcsI RAATTY 1 cut(s) 566
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 3 cut(s) 116, 322, 499
AjiI CACGTC 1 cut(s) 235
AluBI AGCT 2 cut(s) 198, 619
AluI AGCT 2 cut(s) 198, 619
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 214
AlwI GGATC 3 cut(s) 82, 95, 335
AoxI GGCC 2 cut(s) 245, 538
ApeKI GCWGC 2 cut(s) 336, 339
ApoI RAATTY 1 cut(s) 566
AspS9I GGNCC 2 cut(s) 245, 539
AsuHPI GGTGA 1 cut(s) 452
BamHI GGATCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 323, 326
BccI CCATC 3 cut(s) 175, 256, 404
BceAI ACGGC 1 cut(s) 553
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 1 cut(s) 264
BglII AGATCT 2 cut(s) 118, 368
BisI GCNGC 2 cut(s) 337, 340
BlsI GCNGC 2 cut(s) 338, 341
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 245, 539
BmiI GGNNCC 3 cut(s) 89, 246, 639
BpiI GAAGAC 1 cut(s) 688
Bpu10I CCTNAGC 1 cut(s) 689
BpuEI CTTGAG 3 cut(s) 65, 335, 653
BsaBI GATNNNNATC 2 cut(s) 123, 135
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 213, 605
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse8I GATNNNNATC 2 cut(s) 123, 135
BseDI CCNNGG 2 cut(s) 213, 605
BseJI GATNNNNATC 2 cut(s) 123, 135
BseLI CCNNNNNNNGG 1 cut(s) 559
BseXI GCAGC 2 cut(s) 323, 326
BshFI GGCC 2 cut(s) 247, 540
BsiHKAI GWGCWC 1 cut(s) 200
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 247, 540
Bso31I GGTCTC 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 6 cut(s) 87, 118, 124, 136, 327, 368
BspACI CCGC 1 cut(s) 686
BspANI GGCC 2 cut(s) 247, 540
BspLI GGNNCC 3 cut(s) 89, 246, 639
BspPI GGATC 3 cut(s) 82, 95, 335
BspTNI GGTCTC 1 cut(s) 214
BssECI CCNNGG 2 cut(s) 213, 605
BssMI GATC 6 cut(s) 87, 118, 124, 136, 327, 368
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 651
BstC8I GCNNGC 1 cut(s) 656
BstDEI CTNAG 2 cut(s) 365, 689
BstEII GGTNACC 1 cut(s) 275
BstKTI GATC 6 cut(s) 90, 121, 127, 139, 330, 371
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 6 cut(s) 87, 118, 124, 136, 327, 368
BstMWI GCNNNNNNNGC 2 cut(s) 345, 651
BstNSI RCATGY 1 cut(s) 448
BstPI GGTNACC 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 323, 326
BstV2I GAAGAC 1 cut(s) 688
BstX2I RGATCY 4 cut(s) 87, 118, 327, 368
BstYI RGATCY 4 cut(s) 87, 118, 327, 368
BsuRI GGCC 2 cut(s) 247, 540
BtrI CACGTC 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 656
Cfr13I GGNCC 2 cut(s) 245, 539
Csp6I GTAC 1 cut(s) 467
CviAII CATG 4 cut(s) 333, 437, 445, 478
CviJI RGCY 6 cut(s) 198, 247, 540, 552, 604, 619
CviKI_1 RGCY 6 cut(s) 198, 247, 540, 552, 604, 619
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 2 cut(s) 365, 689
DpnI GATC 6 cut(s) 89, 120, 126, 138, 329, 370
DpnII GATC 6 cut(s) 87, 118, 124, 136, 327, 368
Ecl136II GAGCTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 200
Eco31I GGTCTC 1 cut(s) 214
Eco32I GATATC 1 cut(s) 391
Eco53kI GAGCTC 1 cut(s) 198
Eco91I GGTNACC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 275
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 4 cut(s) 336, 440, 448, 481
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 1 cut(s) 548
FatI CATG 4 cut(s) 332, 436, 444, 477
Fnu4HI GCNGC 2 cut(s) 337, 340
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 2 cut(s) 337, 340
FspBI CTAG 1 cut(s) 264
GluI GCNGC 2 cut(s) 337, 340
HaeIII GGCC 2 cut(s) 247, 540
Hin1II CATG 4 cut(s) 336, 440, 448, 481
HinfI GANTC 2 cut(s) 313, 416
HphI GGTGA 1 cut(s) 452
Hpy188I TCNGA 3 cut(s) 232, 528, 609
Hpy188III TCNNGA 3 cut(s) 69, 122, 128
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 3 cut(s) 98, 181, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 345, 651
HpyF3I CTNAG 2 cut(s) 365, 689
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 4 cut(s) 336, 440, 448, 481
Kzo9I GATC 6 cut(s) 87, 118, 124, 136, 327, 368
LmnI GCTCC 1 cut(s) 514
LpnPI CCDG 1 cut(s) 545
Lsp1109I GCAGC 2 cut(s) 323, 326
MaeI CTAG 1 cut(s) 264
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 3 cut(s) 275, 357, 440
MalI GATC 6 cut(s) 89, 120, 126, 138, 329, 370
MboI GATC 6 cut(s) 87, 118, 124, 136, 327, 368
MboII GAAGA 4 cut(s) 58, 128, 511, 693
MflI RGATCY 4 cut(s) 87, 118, 327, 368
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 2 cut(s) 226, 566
MlyI GAGTC 1 cut(s) 322
MnlI CCTC 5 cut(s) 235, 359, 615, 628, 651
MseI TTAA 3 cut(s) 297, 519, 545
Mva1269I GAATGC 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 345, 651
NdeII GATC 6 cut(s) 87, 118, 124, 136, 327, 368
NlaIII CATG 4 cut(s) 336, 440, 448, 481
NlaIV GGNNCC 3 cut(s) 89, 246, 639
NmuCI GTSAC 1 cut(s) 440
NspI RCATGY 1 cut(s) 448
PctI GAATGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 559
PkrI GCNGC 2 cut(s) 338, 341
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 200
PspEI GGTNACC 1 cut(s) 275
PspN4I GGNNCC 3 cut(s) 89, 246, 639
PspPI GGNCC 2 cut(s) 245, 539
PsuI RGATCY 4 cut(s) 87, 118, 327, 368
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SacI GAGCTC 1 cut(s) 200
SaqAI TTAA 3 cut(s) 297, 519, 545
SatI GCNGC 2 cut(s) 337, 340
Sau3AI GATC 6 cut(s) 87, 118, 124, 136, 327, 368
Sau96I GGNCC 2 cut(s) 245, 539
SchI GAGTC 1 cut(s) 322
SduI GDGCHC 1 cut(s) 200
SetI ASST 6 cut(s) 200, 237, 282, 621, 639, 695
SmlI CTYRAG 3 cut(s) 80, 350, 632
SmoI CTYRAG 3 cut(s) 80, 350, 632
Sse9I AATT 2 cut(s) 226, 566
SsiI CCGC 1 cut(s) 686
SspI AATATT 1 cut(s) 53
SspMI CTAG 1 cut(s) 264
SstI GAGCTC 1 cut(s) 200
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 1 cut(s) 237
TaqI TCGA 4 cut(s) 70, 129, 135, 399
TaqII GACCGA 1 cut(s) 196
TasI AATT 2 cut(s) 226, 566
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 3 cut(s) 297, 519, 545
Tru9I TTAA 3 cut(s) 297, 519, 545
TscAI CASTG 1 cut(s) 146
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 336, 339
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 163, 494
TspRI CASTG 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 559
XapI RAATTY 1 cut(s) 566
XceI RCATGY 1 cut(s) 448
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.