pycom07g19450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0024389
Physical Location & Seq
Forward (+)
21806824 .. 21807372
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19450.1

Sequence Viewer

Length: 549 bp
ATGGACCTGACATCCACCTTCTTGGCGTTCACCTTAGGCACAGTGATAGTCAGCACTCCGTTTTCCATCCCCGCCCTCACCTCCTCCACGCTCGCGTTCTCCGGCAGCCGAAACCTCCTCGTGAACGTCCCGCTGCTGCGCTCCACCCGGTGCCACGTGTCCGTCTTCTCCTCCTGCTCCCTCTTCCTCTCCCCGCTGATCTGGAGGATCCGGCCGTCCTCCACCTTAACCTTCACCTCCTCCTTCCTCAGCCCCGGCACGTCCGCCTTGAACACGTGCGCCGTCGGCGTCTCCTTCCAGTCGATGTGCGCGTGGGCGAACGACTCTGTTTCGGACCGGGACGACAGGTCCGAGAGTGAGCGGAACGGGTGGTGGACCTGCTGGTCCCACAGGTCCAGTGAAAACGGGTCGAACACGCCCGATCGGCGCCCGAATGGGTTCGGAATCAGCGACATCGTTCGAGAATCTTCTGATCAATCAGGCAATTTGGAATTTGATTTTTCGGATAAGGGGCGGTTTCGAATTTGGGCGAATTTCATAAGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.07

Weight (kDa)

6.71

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024389)

Species Orthologous Gene IDs
malus_domestica MD08G1068400.v1.1
pyrus_communis pycom07g19450
rosa_roxburghii Rroxscaffold_1G00017350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 382
Acc36I ACCTGC 1 cut(s) 386
AccB1I GGYRCC 2 cut(s) 150, 426
AccBSI CCGCTC 1 cut(s) 361
AccII CGCG 2 cut(s) 95, 311
AciI CCGC 6 cut(s) 72, 131, 194, 264, 361, 514
AclWI GGATC 2 cut(s) 202, 215
AcoI YGGCCR 1 cut(s) 212
AcsI RAATTY 3 cut(s) 491, 522, 532
AcvI CACGTG 2 cut(s) 157, 276
AcyI GRCGYC 2 cut(s) 288, 427
AdeI CACNNNGTG 1 cut(s) 150
AflIII ACRYGT 2 cut(s) 156, 273
AgsI TTSAA 1 cut(s) 271
AhdI GACNNNNNGTC 1 cut(s) 346
AjiI CACGTC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 2 cut(s) 202, 215
AoxI GGCC 1 cut(s) 212
ApeKI GCWGC 3 cut(s) 105, 133, 136
ApoI RAATTY 3 cut(s) 491, 522, 532
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 4 cut(s) 141, 281, 311, 429
AspS9I GGNCC 6 cut(s) 4, 334, 348, 375, 384, 393
AsuC2I CCSGG 3 cut(s) 148, 255, 338
AsuHPI GGTGA 3 cut(s) 22, 70, 226
AsuII TTCGAA 1 cut(s) 520
AvaII GGWCC 6 cut(s) 4, 334, 348, 375, 384, 393
AxyI CCTNAGG 1 cut(s) 34
BamHI GGATCC 1 cut(s) 207
BanI GGYRCC 2 cut(s) 150, 426
BauI CACGAG 1 cut(s) 119
BbrPI CACGTG 2 cut(s) 157, 276
BbsI GAAGAC 1 cut(s) 157
BbvCI CCTCAGC 1 cut(s) 248
BbvI GCAGC 3 cut(s) 117, 120, 123
BccI CCATC 1 cut(s) 74
BceAI ACGGC 2 cut(s) 199, 266
BclI TGATCA 1 cut(s) 472
BcnI CCSGG 3 cut(s) 148, 255, 338
BcoDI GTCTC 1 cut(s) 295
BfoI RGCGCY 1 cut(s) 430
BfuAI ACCTGC 1 cut(s) 386
BglI GCCNNNNNGGC 1 cut(s) 424
BisI GCNGC 3 cut(s) 106, 134, 137
BlsI GCNGC 3 cut(s) 107, 135, 138
Bme1390I CCNGG 3 cut(s) 148, 255, 338
Bme18I GGWCC 6 cut(s) 4, 334, 348, 375, 384, 393
BmeRI GACNNNNNGTC 1 cut(s) 346
BmgBI CACGTC 1 cut(s) 261
BmgT120I GGNCC 6 cut(s) 4, 334, 348, 375, 384, 393
BmiI GGNNCC 4 cut(s) 152, 209, 386, 428
BmrFI CCNGG 3 cut(s) 148, 255, 338
BpiI GAAGAC 1 cut(s) 157
BpmI CTGGAG 1 cut(s) 223
Bpu10I CCTNAGC 1 cut(s) 248
Bpu14I TTCGAA 1 cut(s) 520
BpuMI CCSGG 3 cut(s) 148, 255, 338
BsaAI YACGTR 2 cut(s) 157, 276
BsaHI GRCGYC 2 cut(s) 288, 427
BsaJI CCNNGG 1 cut(s) 253
Bse1I ACTGG 2 cut(s) 298, 396
Bse21I CCTNAGG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 2 cut(s) 11, 66
BseMII CTCAG 1 cut(s) 262
BseNI ACTGG 2 cut(s) 298, 396
BseRI GAGGAG 4 cut(s) 73, 107, 160, 229
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 3 cut(s) 117, 120, 123
Bsh1236I CGCG 2 cut(s) 95, 311
Bsh1285I CGRYCG 2 cut(s) 215, 424
BshFI GGCC 1 cut(s) 214
BshNI GGYRCC 2 cut(s) 150, 426
BsiEI CGRYCG 2 cut(s) 215, 424
BsiSI CCGG 5 cut(s) 102, 148, 211, 255, 337
BslFI GGGAC 3 cut(s) 113, 353, 370
BsmAI GTCTC 1 cut(s) 295
BsmBI CGTCTC 1 cut(s) 295
BsmFI GGGAC 3 cut(s) 113, 353, 370
BsnI GGCC 1 cut(s) 214
Bsp119I TTCGAA 1 cut(s) 520
Bsp143I GATC 4 cut(s) 198, 207, 421, 472
BspACI CCGC 6 cut(s) 72, 131, 194, 264, 361, 514
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 1 cut(s) 261
BspFNI CGCG 2 cut(s) 95, 311
BspLI GGNNCC 4 cut(s) 152, 209, 386, 428
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 2 cut(s) 202, 215
BspT104I TTCGAA 1 cut(s) 520
BspT107I GGYRCC 2 cut(s) 150, 426
BsrBI CCGCTC 1 cut(s) 361
BsrI ACTGG 2 cut(s) 298, 396
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 4 cut(s) 198, 207, 421, 472
BssNI GRCGYC 2 cut(s) 288, 427
BssSI CACGAG 1 cut(s) 119
Bst2BI CACGAG 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 2 cut(s) 288, 427
BstBAI YACGTR 2 cut(s) 157, 276
BstBI TTCGAA 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 34, 248
BstF5I GGATG 2 cut(s) 11, 66
BstFNI CGCG 2 cut(s) 95, 311
BstH2I RGCGCY 1 cut(s) 430
BstHHI GCGC 4 cut(s) 141, 281, 311, 429
BstKTI GATC 4 cut(s) 201, 210, 424, 475
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 4 cut(s) 198, 207, 421, 472
BstMCI CGRYCG 2 cut(s) 215, 424
BstMWI GCNNNNNNNGC 2 cut(s) 285, 424
BstSCI CCNGG 3 cut(s) 146, 253, 336
BstUI CGCG 2 cut(s) 95, 311
BstV1I GCAGC 3 cut(s) 117, 120, 123
BstV2I GAAGAC 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 1 cut(s) 207
BstZI CGGCCG 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 34
BsuRI GGCC 1 cut(s) 214
BtrI CACGTC 1 cut(s) 261
BtsCI GGATG 2 cut(s) 11, 66
BtsIMutI CAGTG 2 cut(s) 48, 403
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 4 cut(s) 141, 281, 311, 429
Cfr13I GGNCC 6 cut(s) 4, 334, 348, 375, 384, 393
CpoI CGGWCCG 1 cut(s) 334
CseI GACGC 1 cut(s) 277
CspI CGGWCCG 1 cut(s) 334
CviJI RGCY 3 cut(s) 108, 214, 252
CviKI_1 RGCY 3 cut(s) 108, 214, 252
DdeI CTNAG 2 cut(s) 34, 248
DinI GGCGCC 1 cut(s) 428
DpnI GATC 4 cut(s) 200, 209, 423, 474
DpnII GATC 4 cut(s) 198, 207, 421, 472
DraIII CACNNNGTG 1 cut(s) 150
DrdI GACNNNNNNGTC 1 cut(s) 382
DriI GACNNNNNGTC 1 cut(s) 346
DseDI GACNNNNNNGTC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 212
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 253
EclXI CGGCCG 1 cut(s) 212
Eco47I GGWCC 6 cut(s) 4, 334, 348, 375, 384, 393
Eco52I CGGCCG 1 cut(s) 212
Eco72I CACGTG 2 cut(s) 157, 276
Eco81I CCTNAGG 1 cut(s) 34
EgeI GGCGCC 1 cut(s) 428
EheI GGCGCC 1 cut(s) 428
Esp3I CGTCTC 1 cut(s) 295
FaiI YATR 1 cut(s) 539
FaqI GGGAC 3 cut(s) 113, 353, 370
FauI CCCGC 3 cut(s) 79, 138, 201
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 106, 134, 137
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 3 cut(s) 106, 134, 137
GlaI GCGC 4 cut(s) 140, 280, 310, 428
GluI GCNGC 3 cut(s) 106, 134, 137
GsuI CTGGAG 1 cut(s) 223
HaeII RGCGCY 1 cut(s) 430
HaeIII GGCC 1 cut(s) 214
HapII CCGG 5 cut(s) 102, 148, 211, 255, 337
HgaI GACGC 1 cut(s) 277
HhaI GCGC 4 cut(s) 141, 281, 311, 429
Hin1I GRCGYC 2 cut(s) 288, 427
Hin6I GCGC 4 cut(s) 139, 279, 309, 427
HinP1I GCGC 4 cut(s) 139, 279, 309, 427
HinfI GANTC 3 cut(s) 323, 444, 464
HpaII CCGG 5 cut(s) 102, 148, 211, 255, 337
HphI GGTGA 3 cut(s) 22, 70, 226
Hpy166II GTNNAC 3 cut(s) 30, 124, 375
Hpy188I TCNGA 5 cut(s) 334, 352, 443, 472, 505
Hpy188III TCNNGA 3 cut(s) 121, 202, 461
Hpy8I GTNNAC 3 cut(s) 30, 124, 375
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 4 cut(s) 28, 241, 253, 304
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 4 cut(s) 126, 156, 260, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 424
HpyF3I CTNAG 2 cut(s) 34, 248
HpySE526I ACGT 4 cut(s) 126, 156, 260, 275
Hsp92I GRCGYC 2 cut(s) 288, 427
HspAI GCGC 4 cut(s) 139, 279, 309, 427
KasI GGCGCC 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 4 cut(s) 198, 207, 421, 472
LmnI GCTCC 2 cut(s) 146, 182
Lsp1109I GCAGC 3 cut(s) 117, 120, 123
MaeII ACGT 4 cut(s) 126, 156, 260, 275
MalI GATC 4 cut(s) 200, 209, 423, 474
MbiI CCGCTC 1 cut(s) 361
MboI GATC 4 cut(s) 198, 207, 421, 472
MboII GAAGA 3 cut(s) 157, 175, 459
MflI RGATCY 1 cut(s) 207
MluCI AATT 4 cut(s) 484, 491, 522, 532
Mly113I GGCGCC 1 cut(s) 427
MlyI GAGTC 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 1 cut(s) 227
MspA1I CMGCKG 2 cut(s) 133, 196
MspI CCGG 5 cut(s) 102, 148, 211, 255, 337
MspR9I CCNGG 3 cut(s) 148, 255, 338
MvnI CGCG 2 cut(s) 95, 311
MwoI GCNNNNNNNGC 2 cut(s) 285, 424
NarI GGCGCC 1 cut(s) 427
NciI CCSGG 3 cut(s) 148, 255, 338
NdeII GATC 4 cut(s) 198, 207, 421, 472
NlaIV GGNNCC 4 cut(s) 152, 209, 386, 428
NspV TTCGAA 1 cut(s) 520
PcsI WCGNNNNNNNCGW 3 cut(s) 308, 348, 447
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 444, 464
PkrI GCNGC 3 cut(s) 107, 135, 138
Ple19I CGATCG 1 cut(s) 424
PleI GAGTC 1 cut(s) 317
PluTI GGCGCC 1 cut(s) 430
PmaCI CACGTG 2 cut(s) 157, 276
PmlI CACGTG 2 cut(s) 157, 276
PpsI GAGTC 1 cut(s) 317
Ppu21I YACGTR 2 cut(s) 157, 276
PspCI CACGTG 2 cut(s) 157, 276
PspN4I GGNNCC 4 cut(s) 152, 209, 386, 428
PspPI GGNCC 6 cut(s) 4, 334, 348, 375, 384, 393
PsuI RGATCY 1 cut(s) 207
PvuI CGATCG 1 cut(s) 424
Rsr2I CGGWCCG 1 cut(s) 334
RsrII CGGWCCG 1 cut(s) 334
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 106, 134, 137
Sau3AI GATC 4 cut(s) 198, 207, 421, 472
Sau96I GGNCC 6 cut(s) 4, 334, 348, 375, 384, 393
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 3 cut(s) 148, 255, 338
SfoI GGCGCC 1 cut(s) 428
SfuI TTCGAA 1 cut(s) 520
SinI GGWCC 6 cut(s) 4, 334, 348, 375, 384, 393
Sse9I AATT 4 cut(s) 484, 491, 522, 532
SsiI CCGC 6 cut(s) 72, 131, 194, 264, 361, 514
SspDI GGCGCC 1 cut(s) 426
StyD4I CCNGG 3 cut(s) 146, 253, 336
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 4 cut(s) 129, 159, 263, 278
TaqI TCGA 4 cut(s) 302, 410, 460, 520
TasI AATT 4 cut(s) 484, 491, 522, 532
TfiI GAWTC 2 cut(s) 444, 464
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 2 cut(s) 48, 403
TseI GCWGC 3 cut(s) 105, 133, 136
TspDTI ATGAA 1 cut(s) 526
TspGWI ACGGA 2 cut(s) 48, 151
TspRI CASTG 2 cut(s) 48, 403
VpaK11BI GGWCC 6 cut(s) 4, 334, 348, 375, 384, 393
XapI RAATTY 3 cut(s) 491, 522, 532
XmnI GAANNNNTTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.