pycom07g24270

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0022429
Physical Location & Seq
Forward (+)
24994277 .. 24997567
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGTCAATAAAATTTGAAAGTGGATTTACACCCATCGCTCACTATGTTTTTTCTGGCCGGTCATCACGAAGACACTCTAGTCCCTTTCAAAAAAAACAGAGTTCCCCACTAAATTCTAAGCTTCTAAACCCCTATAAAAACCCCTTTGATCCTGTAAAAACATATAGTAACAATGTGCAAAGTGACGTCTCCTCTCTGCTGCAATGGCAACACAGACCACCATTACCTTCGATCTCCAAAACCATAACATCCCAACCAGAACAAGTACCGAAGTTACAGTTCAATCCCCACAAAGAAACCCATTTTTCTTTGGCTTTAAAAGAAGCCAACTCCATAGCTGTCATAGCTTTCCCAATGATACTTACCGGCCTGTTGCTTTACTCAAGGTCAATCATCTCCATGCTCTTCCTCGGCCGCCTTGGCGAGCTTGCCTTAGCGGGCGGGTCCCTTGCTGTGGGCTTTGCCAACATCACCGGTTACTCCATACTCTCTGGCCTCGCCATGGGAATGGAACCTATCTGCGGCCAAGCTTTTGGCGCCAGAAAACACACTCTGCTCGGCCTCTCTTTGCAGAGGACGGTCCTCCTCTTACTATTTACATCCTTACCCATTTCTCTTCTCTGGCTGAACATGAAGAAAATCCTCCTTATCTGCGGCCAGGACGAAGCGATCGCTGCAGAAGATCAGTTGTACCTCCTCTATTCTCTCCCTGATCTTCTCGCTCAATCTTTTCTCCACCCTCTGAGAATTTACCTCAGAAGTCAATCCATCACTCTGCCTCTCTCATTGTGTGCAACTCTGGATCGATCATCCTTCACATTCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

276

Amino Acids

30.42

Weight (kDa)

9.65

Isoelectric Point (pI)

58.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 118 - 261 3.6e-32 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022429)

Species Orthologous Gene IDs
malus_domestica MD07G1263600.v1.1 MD07G1263900.v1.1
pyrus_communis pycom07g24250 pycom07g24270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 78
AatII GACGTC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 536
AciI CCGC 5 cut(s) 415, 437, 441, 522, 654
AclWI GGATC 2 cut(s) 143, 810
AcoI YGGCCR 4 cut(s) 55, 412, 523, 655
AcsI RAATTY 3 cut(s) 11, 112, 747
AcyI GRCGYC 2 cut(s) 186, 537
AfaI GTAC 2 cut(s) 267, 692
AfiI CCNNNNNNNGG 3 cut(s) 454, 502, 521
AgeI ACCGGT 1 cut(s) 473
AgsI TTSAA 3 cut(s) 17, 89, 283
AjnI CCWGG 1 cut(s) 657
AluBI AGCT 5 cut(s) 121, 338, 347, 427, 530
AluI AGCT 5 cut(s) 121, 338, 347, 427, 530
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 143, 810
AoxI GGCC 7 cut(s) 55, 367, 412, 493, 523, 559, 655
ApeKI GCWGC 2 cut(s) 199, 674
ApoI RAATTY 3 cut(s) 11, 112, 747
AsiGI ACCGGT 1 cut(s) 473
AsiSI GCGATCGC 1 cut(s) 672
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 444, 580
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 2 cut(s) 444, 580
BanI GGYRCC 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 76
BbvI GCAGC 2 cut(s) 186, 661
BccI CCATC 2 cut(s) 41, 776
BciT130I CCWGG 1 cut(s) 659
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 540
BglI GCCNNNNNGGC 1 cut(s) 420
BisI GCNGC 5 cut(s) 200, 415, 523, 655, 675
BlsI GCNGC 5 cut(s) 201, 416, 524, 656, 676
Bme1390I CCNGG 1 cut(s) 659
Bme18I GGWCC 2 cut(s) 444, 580
BmgT120I GGNCC 2 cut(s) 444, 580
BmiI GGNNCC 4 cut(s) 445, 446, 513, 538
BmrFI CCNGG 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 433
BpuEI CTTGAG 1 cut(s) 367
Bsa29I ATCGAT 1 cut(s) 805
BsaHI GRCGYC 2 cut(s) 186, 537
BsaJI CCNNGG 3 cut(s) 409, 418, 501
BsaWI WCCGGW 1 cut(s) 473
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 502, 521
Bse118I RCCGGY 3 cut(s) 57, 365, 473
Bse3DI GCAATG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 659
BseCI ATCGAT 1 cut(s) 805
BseDI CCNNGG 3 cut(s) 409, 418, 501
BseGI GGATG 3 cut(s) 248, 599, 809
BseLI CCNNNNNNNGG 3 cut(s) 454, 502, 521
BseMI GCAATG 1 cut(s) 209
BseMII CTCAG 2 cut(s) 734, 769
BseRI GAGGAG 3 cut(s) 181, 575, 686
BseX3I CGGCCG 1 cut(s) 412
BseXI GCAGC 2 cut(s) 186, 661
Bsh1285I CGRYCG 2 cut(s) 415, 672
BshFI GGCC 7 cut(s) 57, 369, 414, 495, 525, 561, 657
BshNI GGYRCC 1 cut(s) 536
BshTI ACCGGT 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 805
BsiEI CGRYCG 2 cut(s) 415, 672
BsiSI CCGG 3 cut(s) 58, 366, 474
BslFI GGGAC 2 cut(s) 66, 430
BslI CCNNNNNNNGG 3 cut(s) 454, 502, 521
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 66, 430
BsnI GGCC 7 cut(s) 57, 369, 414, 495, 525, 561, 657
Bsp143I GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 5 cut(s) 415, 437, 441, 522, 654
BspANI GGCC 7 cut(s) 57, 369, 414, 495, 525, 561, 657
BspCNI CTCAG 2 cut(s) 735, 768
BspDI ATCGAT 1 cut(s) 805
BspLI GGNNCC 4 cut(s) 445, 446, 513, 538
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 2 cut(s) 143, 810
BspQI GCTCTTC 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 209
BsrFI RCCGGY 3 cut(s) 57, 365, 473
BssAI RCCGGY 3 cut(s) 57, 365, 473
BssECI CCNNGG 3 cut(s) 409, 418, 501
BssMI GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
BssNI GRCGYC 2 cut(s) 186, 537
BssT1I CCWWGG 2 cut(s) 418, 501
Bst2UI CCWGG 1 cut(s) 659
Bst4CI ACNGT 2 cut(s) 279, 580
Bst6I CTCTTC 2 cut(s) 410, 621
BstACI GRCGYC 2 cut(s) 186, 537
BstC8I GCNNGC 3 cut(s) 425, 429, 439
BstDEI CTNAG 4 cut(s) 117, 433, 743, 755
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 3 cut(s) 248, 599, 809
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 7 cut(s) 151, 234, 672, 685, 715, 805, 809
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
BstMCI CGRYCG 2 cut(s) 415, 672
BstMWI GCNNNNNNNGC 5 cut(s) 205, 344, 420, 536, 674
BstNI CCWGG 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 657
BstSFI CTRYAG 1 cut(s) 675
BstV1I GCAGC 2 cut(s) 186, 661
BstV2I GAAGAC 1 cut(s) 76
BstXI CCANNNNNNTGG 2 cut(s) 508, 533
BstZI CGGCCG 1 cut(s) 412
Bsu15I ATCGAT 1 cut(s) 805
BsuRI GGCC 7 cut(s) 57, 369, 414, 495, 525, 561, 657
BsuTUI ATCGAT 1 cut(s) 805
BtgI CCRYGG 1 cut(s) 501
BtgZI GCGATG 1 cut(s) 19
BtsCI GGATG 3 cut(s) 248, 599, 809
Cac8I GCNNGC 3 cut(s) 425, 429, 439
CfoI GCGC 1 cut(s) 539
Cfr10I RCCGGY 3 cut(s) 57, 365, 473
Cfr13I GGNCC 2 cut(s) 444, 580
ClaI ATCGAT 1 cut(s) 805
Csp6I GTAC 2 cut(s) 266, 691
CspAI ACCGGT 1 cut(s) 473
CviAII CATG 3 cut(s) 400, 502, 631
CviQI GTAC 2 cut(s) 266, 691
DdeI CTNAG 4 cut(s) 117, 433, 743, 755
DinI GGCGCC 1 cut(s) 538
DpnI GATC 7 cut(s) 150, 233, 671, 684, 714, 804, 808
DpnII GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
DraI TTTAAA 1 cut(s) 318
DrdI GACNNNNNNGTC 1 cut(s) 78
DseDI GACNNNNNNGTC 1 cut(s) 78
EaeI YGGCCR 4 cut(s) 55, 412, 523, 655
EagI CGGCCG 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 410, 621
EarI CTCTTC 2 cut(s) 410, 621
EclXI CGGCCG 1 cut(s) 412
Eco130I CCWWGG 2 cut(s) 418, 501
Eco47I GGWCC 2 cut(s) 444, 580
Eco52I CGGCCG 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 657
EcoT14I CCWWGG 2 cut(s) 418, 501
EgeI GGCGCC 1 cut(s) 538
EheI GGCGCC 1 cut(s) 538
ErhI CCWWGG 2 cut(s) 418, 501
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 3 cut(s) 403, 505, 634
FaqI GGGAC 2 cut(s) 66, 430
FatI CATG 3 cut(s) 399, 501, 630
FauI CCCGC 2 cut(s) 430, 434
Fnu4HI GCNGC 5 cut(s) 200, 415, 523, 655, 675
FokI GGATG 3 cut(s) 235, 586, 796
Fsp4HI GCNGC 5 cut(s) 200, 415, 523, 655, 675
FspBI CTAG 1 cut(s) 78
GlaI GCGC 1 cut(s) 538
GluI GCNGC 5 cut(s) 200, 415, 523, 655, 675
HaeII RGCGCY 1 cut(s) 540
HaeIII GGCC 7 cut(s) 57, 369, 414, 495, 525, 561, 657
HapII CCGG 3 cut(s) 58, 366, 474
HhaI GCGC 1 cut(s) 539
Hin1I GRCGYC 2 cut(s) 186, 537
Hin1II CATG 3 cut(s) 403, 505, 634
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HindIII AAGCTT 2 cut(s) 119, 528
HpaII CCGG 3 cut(s) 58, 366, 474
HphI GGTGA 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 744, 758
Hpy188III TCNNGA 2 cut(s) 66, 800
HpyAV CCTTC 2 cut(s) 237, 823
HpyCH4III ACNGT 2 cut(s) 279, 580
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 5 cut(s) 178, 202, 571, 677, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 344, 420, 536, 674
HpyF3I CTNAG 4 cut(s) 117, 433, 743, 755
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 2 cut(s) 186, 537
Hsp92II CATG 3 cut(s) 403, 505, 634
HspAI GCGC 1 cut(s) 537
KasI GGCGCC 1 cut(s) 536
KflI GGGWCCC 1 cut(s) 444
Kzo9I GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
LguI GCTCTTC 1 cut(s) 410
Lsp1109I GCAGC 2 cut(s) 186, 661
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 4 cut(s) 167, 182, 273, 476
MalI GATC 7 cut(s) 150, 233, 671, 684, 714, 804, 808
MboI GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
MboII GAAGA 6 cut(s) 81, 397, 608, 646, 692, 707
MluCI AATT 3 cut(s) 11, 112, 747
Mly113I GGCGCC 1 cut(s) 537
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 2 cut(s) 398, 506
MspI CCGG 3 cut(s) 58, 366, 474
MspR9I CCNGG 1 cut(s) 659
MvaI CCWGG 1 cut(s) 659
MwoI GCNNNNNNNGC 5 cut(s) 205, 344, 420, 536, 674
NarI GGCGCC 1 cut(s) 537
NcoI CCATGG 1 cut(s) 501
NdeII GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
NlaIII CATG 3 cut(s) 403, 505, 634
NlaIV GGNNCC 4 cut(s) 445, 446, 513, 538
NmeAIII GCCGAG 2 cut(s) 390, 537
NmuCI GTSAC 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 410
PinAI ACCGGT 1 cut(s) 473
PkrI GCNGC 5 cut(s) 201, 416, 524, 656, 676
Ple19I CGATCG 1 cut(s) 672
PluTI GGCGCC 1 cut(s) 540
PpuMI RGGWCCY 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 657
PspGI CCWGG 1 cut(s) 657
PspN4I GGNNCC 4 cut(s) 445, 446, 513, 538
PspPI GGNCC 2 cut(s) 444, 580
PspPPI RGGWCCY 1 cut(s) 444
PstI CTGCAG 1 cut(s) 679
PvuI CGATCG 1 cut(s) 672
RgaI GCGATCGC 1 cut(s) 672
RsaI GTAC 2 cut(s) 267, 692
RsaNI GTAC 2 cut(s) 266, 691
RseI CAYNNNNRTG 2 cut(s) 398, 506
SapI GCTCTTC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 5 cut(s) 200, 415, 523, 655, 675
Sau3AI GATC 7 cut(s) 148, 231, 669, 682, 712, 802, 806
Sau96I GGNCC 2 cut(s) 444, 580
ScrFI CCNGG 1 cut(s) 659
SfaAI GCGATCGC 1 cut(s) 672
SfcI CTRYAG 1 cut(s) 675
SfoI GGCGCC 1 cut(s) 538
SgfI GCGATCGC 1 cut(s) 672
SinI GGWCC 2 cut(s) 444, 580
SmiMI CAYNNNNRTG 2 cut(s) 398, 506
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 3 cut(s) 11, 112, 747
SsiI CCGC 5 cut(s) 415, 437, 441, 522, 654
SspDI GGCGCC 1 cut(s) 536
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 657
StyI CCWWGG 2 cut(s) 418, 501
TaaI ACNGT 2 cut(s) 279, 580
TaiI ACGT 1 cut(s) 189
TaqI TCGA 2 cut(s) 230, 805
TasI AATT 3 cut(s) 11, 112, 747
TauI GCSGC 3 cut(s) 417, 525, 657
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 2 cut(s) 199, 674
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 1 cut(s) 647
VpaK11BI GGWCC 2 cut(s) 444, 580
XapI RAATTY 3 cut(s) 11, 112, 747
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.