pycom08g00470

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0021849
Physical Location & Seq
Forward (+)
437793 .. 439181
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGGGCCTTGGCTTAACGGCCCAGTTTCATTTCCAAATTCCAGAGCGACCTTCCCAGTGGTCCAATCCAAACCTCTGTGGCGGGGGGCGGTGGGGTCCTTCGTGTCTACCAGCGAGAATGGAGGTGGATTGGACCCAACTACCACCAGAACTCGCCGAATCAATCTCCAAAAAGCTCACAATCTACGCCGACTACCTCCGATTCCGGGTGGTGTGTCACAGCTGGCGAGCCTCCGTCCCCAAAACCCCGCACCACCTCCCTCCCCAGCTCCCTTGGCTGATGCTCCCCCAATCCCAGCCCAACCAATCCCACCGCGCCTTCTTCAACATCTCTAACAGCAGAGTCCACTTCCTCCACCTTCCAGAAGCTTCTCACCGTAAGCGCCGCTGCGGCTCCTCCCACGGATGGCTCGTCATCCTCGACGAAACCCCTTCCGTCCTCCTCGTTAACCCGCTCACGCGCGCCAAGCGCCACCTCCCTCCGCTCTCCACCTTCCCCAACGTTGTTCGATTTGATTACTCCGATGTTGGCCGAGAATACGCCCTCGTATCCCCGTCTGGTGACGTCTACACGCGCAGCCTGACCCAGATGCGCGATTCGTTTCTGAAAAAGGTGGTCCTTTCTTCGAGTCCTCTGGAAGCCAGGGGCTTCACTTTCACCGCCGTAGCGATTGTCAATCAAACCGGCGACTTGGCGTTTTGCAGGGACGGAGACCAGACCTGGACTTTTATCGACGGCGCACAGTCGTATTCCGAGGATGTCGTATCTGTCAACGGTTTGTTTTACGCCGTCGACAAGAAAGGCACCGTGGCGGAGTGCGATGTTAATGGTCCGTCTCCGCCTAGGGTCAGAGGTGGGACAAGGTTGAGAACTTGGGTGATAGGATGGTGTTTATCGGCGAAAACTCATCGTTTTCATTGTCGGCGTCTGATTTTCCGGGAAGCCTTGGAAACTGCGTTTATTTCACAGACGATTATTCGGAATCAAACAATGAGAGCGGAGTCTGGGGATATGATTCGGGCATTTTCAAATTGTGGGATGGAACAATTCAAGAGTTGCCCCCTTATCCAAGGAATTCGAATTATGAGGTGCATTGGCCTGCCGGCTCTCTACCTCTTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

375

Amino Acids

42.22

Weight (kDa)

9.5

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 107 - 283 1.8e-34 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021849)

Species Orthologous Gene IDs
malus_domestica MD08G1006100.v1.1 MD15G1005500.v1.1
prunus_persica Prupe.1G358000_v2.0.a1
pyrus_communis pycom08g00470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 803
AccBSI CCGCTC 3 cut(s) 454, 484, 998
AccI GTMKAC 3 cut(s) 106, 567, 792
AccII CGCG 5 cut(s) 315, 460, 462, 574, 594
AclI AACGTT 1 cut(s) 501
AcoI YGGCCR 1 cut(s) 529
AcsI RAATTY 2 cut(s) 36, 1074
AcyI GRCGYC 2 cut(s) 564, 925
AfiI CCNNNNNNNGG 2 cut(s) 205, 405
AgsI TTSAA 3 cut(s) 325, 1029, 1051
AjnI CCWGG 2 cut(s) 641, 719
AluBI AGCT 4 cut(s) 175, 222, 268, 368
AluI AGCT 4 cut(s) 175, 222, 268, 368
Alw26I GTCTC 2 cut(s) 705, 840
AoxI GGCC 4 cut(s) 4, 18, 529, 1096
ApeKI GCWGC 2 cut(s) 387, 576
ApoI RAATTY 2 cut(s) 36, 1074
AspA2I CCTAGG 1 cut(s) 842
AspLEI GCGC 8 cut(s) 317, 384, 462, 464, 471, 576, 594, 740
AspS9I GGNCC 7 cut(s) 4, 19, 60, 95, 132, 616, 830
AsuC2I CCSGG 2 cut(s) 206, 938
AsuHPI GGTGA 4 cut(s) 365, 572, 649, 889
AsuII TTCGAA 1 cut(s) 1078
AvaII GGWCC 5 cut(s) 60, 95, 132, 616, 830
AvrII CCTAGG 1 cut(s) 842
BanI GGYRCC 1 cut(s) 803
BbvI GCAGC 2 cut(s) 374, 588
BccI CCATC 3 cut(s) 399, 879, 1033
BceAI ACGGC 4 cut(s) 33, 647, 751, 773
BciT130I CCWGG 2 cut(s) 643, 721
BciVI GTATCC 1 cut(s) 559
BcnI CCSGG 2 cut(s) 206, 938
BcoDI GTCTC 2 cut(s) 705, 840
BfaI CTAG 1 cut(s) 843
BfoI RGCGCY 2 cut(s) 385, 472
BfuI GTATCC 1 cut(s) 559
BglI GCCNNNNNGGC 1 cut(s) 390
BisI GCNGC 4 cut(s) 385, 388, 391, 577
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 4 cut(s) 386, 389, 392, 578
Bme1390I CCNGG 4 cut(s) 206, 643, 721, 938
Bme18I GGWCC 5 cut(s) 60, 95, 132, 616, 830
BmgT120I GGNCC 7 cut(s) 4, 19, 60, 95, 132, 616, 830
BmiI GGNNCC 4 cut(s) 96, 134, 394, 805
BmrFI CCNGG 4 cut(s) 206, 643, 721, 938
BmrI ACTGGG 2 cut(s) 16, 49
BmsI GCATC 2 cut(s) 270, 579
BmuI ACTGGG 2 cut(s) 16, 49
Bpu14I TTCGAA 1 cut(s) 1078
BpuMI CCSGG 2 cut(s) 206, 938
BsaHI GRCGYC 2 cut(s) 564, 925
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 9 cut(s) 7, 272, 400, 642, 753, 807, 842, 945, 1069
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 405
Bse118I RCCGGY 2 cut(s) 683, 1102
Bse1I ACTGG 2 cut(s) 22, 55
BseBI CCWGG 2 cut(s) 643, 721
BseDI CCNNGG 9 cut(s) 7, 272, 400, 642, 753, 807, 842, 945, 1069
BseGI GGATG 5 cut(s) 410, 414, 763, 890, 1044
BseLI CCNNNNNNNGG 2 cut(s) 205, 405
BseNI ACTGG 2 cut(s) 22, 55
BsePI GCGCGC 1 cut(s) 460
BseRI GAGGAG 2 cut(s) 385, 431
BseXI GCAGC 2 cut(s) 374, 588
BseYI CCCAGC 2 cut(s) 264, 294
Bsh1236I CGCG 5 cut(s) 315, 460, 462, 574, 594
BshFI GGCC 4 cut(s) 6, 20, 531, 1098
BshNI GGYRCC 1 cut(s) 803
BsiSI CCGG 4 cut(s) 205, 684, 937, 1103
BslFI GGGAC 3 cut(s) 221, 719, 871
BslI CCNNNNNNNGG 2 cut(s) 205, 405
BsmAI GTCTC 2 cut(s) 705, 840
BsmBI CGTCTC 1 cut(s) 840
BsmFI GGGAC 3 cut(s) 221, 719, 871
BsnI GGCC 4 cut(s) 6, 20, 531, 1098
Bso31I GGTCTC 1 cut(s) 705
Bsp119I TTCGAA 1 cut(s) 1078
BspANI GGCC 4 cut(s) 6, 20, 531, 1098
BspFNI CGCG 5 cut(s) 315, 460, 462, 574, 594
BspLI GGNNCC 4 cut(s) 96, 134, 394, 805
BspT104I TTCGAA 1 cut(s) 1078
BspT107I GGYRCC 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 705
BsrBI CCGCTC 3 cut(s) 454, 484, 998
BsrFI RCCGGY 2 cut(s) 683, 1102
BsrI ACTGG 2 cut(s) 22, 55
BssAI RCCGGY 2 cut(s) 683, 1102
BssECI CCNNGG 9 cut(s) 7, 272, 400, 642, 753, 807, 842, 945, 1069
BssHII GCGCGC 1 cut(s) 460
BssNI GRCGYC 2 cut(s) 564, 925
BssT1I CCWWGG 5 cut(s) 7, 272, 842, 945, 1069
Bst2UI CCWGG 2 cut(s) 643, 721
Bst4CI ACNGT 4 cut(s) 377, 744, 776, 808
BstACI GRCGYC 2 cut(s) 564, 925
BstBI TTCGAA 1 cut(s) 1078
BstC8I GCNNGC 5 cut(s) 224, 228, 462, 1100, 1104
BstDSI CCRYGG 2 cut(s) 400, 807
BstF5I GGATG 5 cut(s) 410, 414, 763, 890, 1044
BstFNI CGCG 5 cut(s) 315, 460, 462, 574, 594
BstH2I RGCGCY 2 cut(s) 385, 472
BstHHI GCGC 8 cut(s) 317, 384, 462, 464, 471, 576, 594, 740
BstMAI GTCTC 2 cut(s) 705, 840
BstMWI GCNNNNNNNGC 4 cut(s) 274, 390, 466, 468
BstNI CCWGG 2 cut(s) 643, 721
BstSCI CCNGG 4 cut(s) 204, 641, 719, 936
BstUI CGCG 5 cut(s) 315, 460, 462, 574, 594
BstV1I GCAGC 2 cut(s) 374, 588
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 4 cut(s) 6, 20, 531, 1098
BtgI CCRYGG 2 cut(s) 400, 807
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 5 cut(s) 410, 414, 763, 890, 1044
BtsIMutI CAGTG 1 cut(s) 62
Cac8I GCNNGC 5 cut(s) 224, 228, 462, 1100, 1104
CfoI GCGC 8 cut(s) 317, 384, 462, 464, 471, 576, 594, 740
Cfr10I RCCGGY 2 cut(s) 683, 1102
Cfr13I GGNCC 7 cut(s) 4, 19, 60, 95, 132, 616, 830
CseI GACGC 1 cut(s) 914
EaeI YGGCCR 1 cut(s) 529
EciI GGCGGA 2 cut(s) 827, 828
Eco130I CCWWGG 5 cut(s) 7, 272, 842, 945, 1069
Eco31I GGTCTC 1 cut(s) 705
Eco47I GGWCC 5 cut(s) 60, 95, 132, 616, 830
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 1074
EcoRII CCWGG 2 cut(s) 641, 719
EcoT14I CCWWGG 5 cut(s) 7, 272, 842, 945, 1069
ErhI CCWWGG 5 cut(s) 7, 272, 842, 945, 1069
Esp3I CGTCTC 1 cut(s) 840
FaiI YATR 2 cut(s) 1013, 1085
FaqI GGGAC 3 cut(s) 221, 719, 871
FauI CCCGC 3 cut(s) 74, 255, 459
FblI GTMKAC 3 cut(s) 106, 567, 792
Fnu4HI GCNGC 4 cut(s) 385, 388, 391, 577
FokI GGATG 5 cut(s) 401, 417, 770, 897, 1051
Fsp4HI GCNGC 4 cut(s) 385, 388, 391, 577
FspBI CTAG 1 cut(s) 843
GlaI GCGC 8 cut(s) 316, 383, 461, 463, 470, 575, 593, 739
GluI GCNGC 4 cut(s) 385, 388, 391, 577
GsaI CCCAGC 2 cut(s) 268, 298
HaeII RGCGCY 2 cut(s) 385, 472
HaeIII GGCC 4 cut(s) 6, 20, 531, 1098
HapII CCGG 4 cut(s) 205, 684, 937, 1103
HgaI GACGC 1 cut(s) 914
HhaI GCGC 8 cut(s) 317, 384, 462, 464, 471, 576, 594, 740
Hin1I GRCGYC 2 cut(s) 564, 925
Hin6I GCGC 8 cut(s) 315, 382, 460, 462, 469, 574, 592, 738
HinP1I GCGC 8 cut(s) 315, 382, 460, 462, 469, 574, 592, 738
HincII GTYRAC 3 cut(s) 448, 772, 793
HindII GTYRAC 3 cut(s) 448, 772, 793
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 8 cut(s) 158, 201, 342, 596, 628, 982, 1001, 1015
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 4 cut(s) 205, 684, 937, 1103
HphI GGTGA 4 cut(s) 365, 572, 649, 889
Hpy166II GTNNAC 6 cut(s) 107, 346, 448, 568, 772, 793
Hpy188I TCNGA 7 cut(s) 200, 523, 606, 754, 851, 930, 981
Hpy188III TCNNGA 4 cut(s) 41, 362, 635, 1051
Hpy8I GTNNAC 6 cut(s) 107, 346, 448, 568, 772, 793
Hpy99I CGWCG 3 cut(s) 425, 737, 794
HpyAV CCTTC 6 cut(s) 60, 108, 328, 368, 441, 502
HpyCH4III ACNGT 4 cut(s) 377, 744, 776, 808
HpyCH4IV ACGT 2 cut(s) 501, 564
HpyCH4V TGCA 2 cut(s) 702, 1092
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 390, 466, 468
HpySE526I ACGT 2 cut(s) 501, 564
Hsp92I GRCGYC 2 cut(s) 564, 925
HspAI GCGC 8 cut(s) 315, 382, 460, 462, 469, 574, 592, 738
KroI GCCGGC 1 cut(s) 1102
KroNI GCCGGC 1 cut(s) 1104
KspAI GTTAAC 1 cut(s) 448
LmnI GCTCC 3 cut(s) 273, 288, 398
Lsp1109I GCAGC 2 cut(s) 374, 588
LweI GCATC 2 cut(s) 270, 579
MaeI CTAG 1 cut(s) 843
MaeII ACGT 2 cut(s) 501, 564
MaeIII GTNAC 2 cut(s) 215, 560
MbiI CCGCTC 3 cut(s) 454, 484, 998
MboII GAAGA 2 cut(s) 313, 615
MluCI AATT 5 cut(s) 36, 1030, 1046, 1074, 1080
MlyI GAGTC 3 cut(s) 351, 637, 1010
MroNI GCCGGC 1 cut(s) 1102
MseI TTAA 3 cut(s) 14, 447, 825
MspA1I CMGCKG 2 cut(s) 222, 387
MspI CCGG 4 cut(s) 205, 684, 937, 1103
MspR9I CCNGG 4 cut(s) 206, 643, 721, 938
MvaI CCWGG 2 cut(s) 643, 721
MvnI CGCG 5 cut(s) 315, 460, 462, 574, 594
MwoI GCNNNNNNNGC 4 cut(s) 274, 390, 466, 468
NaeI GCCGGC 1 cut(s) 1104
NciI CCSGG 2 cut(s) 206, 938
NgoMIV GCCGGC 1 cut(s) 1102
NlaIV GGNNCC 4 cut(s) 96, 134, 394, 805
NmeAIII GCCGAG 1 cut(s) 557
NmuCI GTSAC 2 cut(s) 215, 560
NspV TTCGAA 1 cut(s) 1078
PauI GCGCGC 1 cut(s) 460
PcsI WCGNNNNNNNCGW 2 cut(s) 408, 417
PdiI GCCGGC 1 cut(s) 1104
PfeI GAWTC 5 cut(s) 158, 201, 596, 982, 1015
PfoI TCCNGGA 1 cut(s) 936
PkrI GCNGC 4 cut(s) 386, 389, 392, 578
PleI GAGTC 3 cut(s) 350, 636, 1009
PpsI GAGTC 3 cut(s) 350, 636, 1009
PpuMI RGGWCCY 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 641, 719
PspFI CCCAGC 2 cut(s) 264, 294
PspGI CCWGG 2 cut(s) 641, 719
PspN4I GGNNCC 4 cut(s) 96, 134, 394, 805
PspPI GGNCC 7 cut(s) 4, 19, 60, 95, 132, 616, 830
PspPPI RGGWCCY 1 cut(s) 95
PteI GCGCGC 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 222
SalI GTCGAC 1 cut(s) 791
SaqAI TTAA 3 cut(s) 14, 447, 825
SatI GCNGC 4 cut(s) 385, 388, 391, 577
Sau96I GGNCC 7 cut(s) 4, 19, 60, 95, 132, 616, 830
SchI GAGTC 3 cut(s) 351, 637, 1010
ScrFI CCNGG 4 cut(s) 206, 643, 721, 938
SfaNI GCATC 2 cut(s) 270, 579
SfuI TTCGAA 1 cut(s) 1078
SinI GGWCC 5 cut(s) 60, 95, 132, 616, 830
Sse9I AATT 5 cut(s) 36, 1030, 1046, 1074, 1080
SspMI CTAG 1 cut(s) 843
StyD4I CCNGG 4 cut(s) 204, 641, 719, 936
StyI CCWWGG 5 cut(s) 7, 272, 842, 945, 1069
TaaI ACNGT 4 cut(s) 377, 744, 776, 808
TaiI ACGT 2 cut(s) 504, 567
TaqI TCGA 6 cut(s) 420, 508, 626, 732, 792, 1078
TasI AATT 5 cut(s) 36, 1030, 1046, 1074, 1080
TauI GCSGC 2 cut(s) 387, 393
TfiI GAWTC 5 cut(s) 158, 201, 596, 982, 1015
Tru1I TTAA 3 cut(s) 14, 447, 825
Tru9I TTAA 3 cut(s) 14, 447, 825
TscAI CASTG 1 cut(s) 62
TseFI GTSAC 2 cut(s) 215, 560
TseI GCWGC 2 cut(s) 387, 576
Tsp45I GTSAC 2 cut(s) 215, 560
TspDTI ATGAA 2 cut(s) 17, 905
TspGWI ACGGA 5 cut(s) 223, 417, 424, 723, 822
TspRI CASTG 1 cut(s) 62
VpaK11BI GGWCC 5 cut(s) 60, 95, 132, 616, 830
XapI RAATTY 2 cut(s) 36, 1074
XmaJI CCTAGG 1 cut(s) 842
XmiI GTMKAC 3 cut(s) 106, 567, 792
XspI CTAG 1 cut(s) 843
ZraI GACGTC 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.