pycom08g03610

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0019185
Physical Location & Seq
Forward (+)
2801322 .. 2803713
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03610.1

Sequence Viewer

Length: 2145 bp
ATGCCGAGCCGAGCCGAGCCGTCGCGCGCCGGCGCAGGTCCTCGACGCAGGGGCGTCGAGACGAGCGAGTCGCTTTGGGGAGGAGTAACTCGTCAGTCAGTGAGTCGGTTTGAATTGGGATTCTCGATTTCCTATTTGATTTGGGATTTTCAGTCTCCTATAAATTGTGATTTTCGTAACCCTAATCAGAAATCACACCTCTCATTCTTTCCTTTCAGAATAATGGCGAAGGACAACAATGGCTACAAGGACACCGATGACAAGCTGCTGCGGGCGAAAAGGGTTAAGAACAAAGCCCCAGCCCCTGTCCAAATCACCGCAGAGCAACTCCTCCGCGAGGCGCGTGAGGGTCAGGAGGGGGAAATCCGTCCTCCCGAACAGCAAATAAATGACGCTACCGAGCTTGCTGATTTTCGCCTCCGAATGCGCAAGGGATTCGAGGACCAAGTCCGGCGCCAAAAGCAGAATGCCCGCGTCTGGATCAAGTATGCTCGGTGGGAGGAGTCCCAGAAGGAATTGGACCGTGCACGCTCTGTTTGGGAGCGGCAGGAGATGAAGAATAAGATGATCAACCATGCGAGGAATGTGTGGGAGCGTGCTGTGCAACTCTTGCCGAGGGTCGATCAACTCTGGTACAAATATATTCACATGGAGGAGATGATAGGGAATGTGGCTCGGGCTCGGGAAATATTTCGGAGGTGGATGGATTGGATGCCGGACCAGCAAGGCTGGATCGGATTCATCGAGTTTGAGCTTCGGTATAATGAAGTAGACCACGCGAGAGCGATTTTTGAGCGGTTTGTTCAATGTCATCCAAAAGCTGACGCTTGGATTCGGTATGCCAAGTTTGAGATGAAGAATGGTGACCTTGCAAGGGTAAGGGATGTGTATCAGACGGCGGTGGATATGCTAGACAACGATAAGGAGGCCGAACAGTTGTTTCTGGCTTTTGCTGAATTTGAAGAAGGGTGCAAAGAAATCGAGCGTGCAAGGAGTATTTATAAGTTAGGGCGCGATCGTATACCAAAAGGAATGGCTGCTGCTTTGTATAGGATGTTCGAGGAATTTGAGGAGCGTTATGGGGACAGACAAGGGATCGAGGATGCAGTTGTGAACAAGGCAAGGTTCAAGTACGAGCAGGAGGTTAGGAAGGATCCTTTGGATTATGATGCCTGGTTTGGTTATATAAGGCTAGAAGAAAATGCAGGGAACAAGGAGAAGATTAGACGAGTTTATGATCGAGCGATTGCCAATGTACCACCGGCTCAAGAAAAGCGGTATTGGCAGTGCTACATTTATCTATGGATCAACTATGCATTGTATGAGGAGATTGATGCTTGCGATGAGGACCGTGCACGCGCTGTCTATAGAATGTGCCTTGAACTGATTCCTCATAAGAAGTTTACATTTGCAAAAATGTGGATTCTCGCAGCCGAGTTTGAGATCCGACAACTGAAACTCGAGTCTGCACGTAAGATACTTGGTACTGCAATTGGCAAGGCAGCTAAAGGCAAGATATTTAAGAAGTACATTGAGATTGAGTGGCAACTATGTAATGCTGATCGCTGTCGCAAACTGTATGAAAAGTATCTGCATTGGTCGCCGGAAAATTGCTACGCGTGGATCAGGTATGCAGAGTTTGAGAAAAGAATTTGCGACACAGAACGAGCCAGATCGGTTTATGAACTTGCCATCGGCCAAAAACAACTCGACAAGCCCGAATTGCTGTGGAAGTCATACATTGACTTTGAACTAGCAGAGAGAGAGTTTGAGAGAGCTAGAAATCTGTACGGGCGACTATTAGAACGGACCCGACACGTTAAAGTATGGATCAGTTACGCAGCATTCGAGGCATCGGCTATGGTGGGAGGAGACGGAATGCGCTCAGAAGATGTTATTGAGCAAAAGGAACAATGCATTCAGCGCGCCAGAAGGGTTTTCGAGAGAGCGTCGGACTATTTTAGGACTTCAGCACCGGAACTTAAGGAACAAAGAAGTATGCTGCTAGAACAATGGCTGAACATGGAGAAGGAGTTTGGGGATTTCGGTGATGTTACGTTGGTGCAAGCGAAGCTGCCGCGGAGATTGAAGAGGAAGAGGCCAATAGTTATTGAAGATGGTGTTTCTGCTGGGTTTGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf TPR_8

Protein Analysis

715

Amino Acids

85.26

Weight (kDa)

8.85

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAT_Syf1_CNRKL1_N PF23233 186 - 273 2.2e-10 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_Syf1_CNRKL1_C PF23231 188 - 289 1.2e-06 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N PF23240 222 - 335 3.4e-11 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 242 - 349 1.2e-13 PRP39 C-terminal HAT repeat
HAT PF02184 256 - 284 8.2e-10 HAT (Half-A-TPR) repeat
HAT_Syf1_CNRKL1_C PF23231 290 - 365 1.6e-08 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 376 - 536 4.9e-10 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N PF23240 377 - 485 5.5e-06 PRP39 N-terminal HAT repeat
HAT_PRP39_N PF23240 507 - 602 5e-11 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 569 - 676 2e-07 PRP39 C-terminal HAT repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51110
malus_domestica MD08G1044800.v1.1 MD08G1045500.v1.1
pyrus_communis pycom08g03610 pycom08g03630 pycom08g03750 pycom08g03760
rosa_samantha Rh5BG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1002
AasI GACNNNNNNGTC 1 cut(s) 67
Acc16I TGCGCA 1 cut(s) 428
Acc36I ACCTGC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 453
AccBSI CCGCTC 2 cut(s) 544, 796
AccI GTMKAC 2 cut(s) 771, 1021
AclWI GGATC 9 cut(s) 488, 740, 1103, 1148, 1161, 1313, 1438, 1631, 1838
AcoI YGGCCR 1 cut(s) 1696
AcsI RAATTY 3 cut(s) 956, 1064, 1650
AcuI CTGAAG 1 cut(s) 1953
AcyI GRCGYC 2 cut(s) 54, 454
AfaI GTAC 6 cut(s) 635, 1133, 1257, 1486, 1529, 1790
AfiI CCNNNNNNNGG 3 cut(s) 337, 477, 874
AflII CTTAAG 1 cut(s) 1982
AflIII ACRYGT 2 cut(s) 1617, 1816
AgsI TTSAA 9 cut(s) 113, 806, 962, 1129, 1382, 1751, 2089, 2114, 2138
AjnI CCWGG 1 cut(s) 1172
AjuI GAANNNNNNNTTGG 2 cut(s) 1142, 1174
AluBI AGCT 7 cut(s) 265, 403, 754, 821, 1505, 1778, 2074
AluI AGCT 7 cut(s) 265, 403, 754, 821, 1505, 1778, 2074
Alw21I GWGCWC 2 cut(s) 529, 1357
Alw26I GTCTC 3 cut(s) 53, 159, 1866
Alw44I GTGCAC 2 cut(s) 525, 1353
AlwI GGATC 9 cut(s) 488, 740, 1103, 1148, 1161, 1313, 1438, 1631, 1838
AlwNI CAGNNNCTG 1 cut(s) 305
Ama87I CYCGRG 3 cut(s) 675, 681, 1460
AoxI GGCC 3 cut(s) 927, 1696, 2099
ApaLI GTGCAC 2 cut(s) 525, 1353
ApeKI GCWGC 9 cut(s) 265, 268, 1037, 1040, 1430, 1502, 1841, 2002, 2074
ApoI RAATTY 3 cut(s) 956, 1064, 1650
Asp700I GAANNNNTTC 2 cut(s) 690, 1386
AspS9I GGNCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
AsuHPI GGTGA 3 cut(s) 307, 875, 2060
AvaI CYCGRG 3 cut(s) 675, 681, 1460
AvaII GGWCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
BaeGI GKGCMC 2 cut(s) 529, 1357
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 682
Bbv12I GWGCWC 2 cut(s) 529, 1357
BbvI GCAGC 9 cut(s) 252, 255, 1024, 1027, 1442, 1514, 1853, 1989, 2061
BccI CCATC 3 cut(s) 697, 1700, 2111
BceAI ACGGC 2 cut(s) 4, 912
BcgI CGANNNNNNTGC 6 cut(s) 37, 71, 418, 452, 961, 995
BciT130I CCWGG 1 cut(s) 1174
BclI TGATCA 1 cut(s) 567
BcoDI GTCTC 3 cut(s) 53, 159, 1866
BfaI CTAG 5 cut(s) 911, 1193, 1754, 1779, 2006
BfmI CTRYAG 1 cut(s) 1366
BfoI RGCGCY 1 cut(s) 457
BfrI CTTAAG 1 cut(s) 1982
BfuAI ACCTGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 1174
Bme18I GGWCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
BmeT110I CYCGRG 3 cut(s) 675, 681, 1460
BmgT120I GGNCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
BmiI GGNNCC 3 cut(s) 455, 1155, 1811
BmrFI CCNGG 1 cut(s) 1174
BmsI GCATC 5 cut(s) 702, 1093, 1159, 1324, 1862
BpuEI CTTGAG 1 cut(s) 1251
BsaAI YACGTR 1 cut(s) 1472
BsaBI GATNNNNATC 1 cut(s) 888
BsaHI GRCGYC 2 cut(s) 54, 454
BsaJI CCNNGG 2 cut(s) 614, 2078
BsaWI WCCGGW 1 cut(s) 1975
BsaXI ACNNNNNCTCC 2 cut(s) 2018, 2048
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 477, 874
Bse118I RCCGGY 2 cut(s) 29, 1261
Bse8I GATNNNNATC 1 cut(s) 888
BseBI CCWGG 1 cut(s) 1174
BseDI CCNNGG 2 cut(s) 614, 2078
BseGI GGATG 6 cut(s) 708, 717, 811, 889, 1059, 1108
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 3 cut(s) 337, 477, 874
BseMII CTCAG 1 cut(s) 1899
BsePI GCGCGC 2 cut(s) 25, 1924
BseRI GAGGAG 7 cut(s) 96, 320, 515, 668, 1085, 1340, 1884
BseSI GKGCMC 2 cut(s) 529, 1357
BseXI GCAGC 9 cut(s) 252, 255, 1024, 1027, 1442, 1514, 1853, 1989, 2061
BseYI CCCAGC 2 cut(s) 298, 2129
BsgI GTGCAG 1 cut(s) 1452
Bsh1285I CGRYCG 1 cut(s) 1018
BshFI GGCC 3 cut(s) 929, 1698, 2101
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 1018
BsiHKAI GWGCWC 2 cut(s) 529, 1357
BsiHKCI CYCGRG 3 cut(s) 675, 681, 1460
BsiSI CCGG 6 cut(s) 30, 451, 716, 1262, 1604, 1976
BslFI GGGAC 2 cut(s) 490, 1097
BslI CCNNNNNNNGG 3 cut(s) 337, 477, 874
BsmAI GTCTC 3 cut(s) 53, 159, 1866
BsmBI CGTCTC 2 cut(s) 53, 1866
BsmFI GGGAC 2 cut(s) 490, 1097
BsmI GAATGC 5 cut(s) 429, 472, 1844, 1884, 1917
BsnI GGCC 3 cut(s) 929, 1698, 2101
BsoBI CYCGRG 3 cut(s) 675, 681, 1460
Bsp1286I GDGCHC 3 cut(s) 529, 682, 1357
BspANI GGCC 3 cut(s) 929, 1698, 2101
BspCNI CTCAG 1 cut(s) 1898
BspLI GGNNCC 3 cut(s) 455, 1155, 1811
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 9 cut(s) 488, 740, 1103, 1148, 1161, 1313, 1438, 1631, 1838
BspT107I GGYRCC 1 cut(s) 453
BspTI CTTAAG 1 cut(s) 1982
BsrBI CCGCTC 2 cut(s) 544, 796
BsrFI RCCGGY 2 cut(s) 29, 1261
BssAI RCCGGY 2 cut(s) 29, 1261
BssECI CCNNGG 2 cut(s) 614, 2078
BssHII GCGCGC 2 cut(s) 25, 1924
BssNAI GTATAC 1 cut(s) 1022
BssNI GRCGYC 2 cut(s) 54, 454
Bst1107I GTATAC 1 cut(s) 1022
Bst2UI CCWGG 1 cut(s) 1174
Bst4CI ACNGT 4 cut(s) 524, 936, 1352, 1578
Bst6I CTCTTC 2 cut(s) 2084, 2090
BstACI GRCGYC 2 cut(s) 54, 454
BstAFI CTTAAG 1 cut(s) 1982
BstAPI GCANNNNNTGC 1 cut(s) 610
BstBAI YACGTR 1 cut(s) 1472
BstDEI CTNAG 1 cut(s) 1885
BstDSI CCRYGG 1 cut(s) 2078
BstEII GGTNACC 1 cut(s) 863
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 6 cut(s) 708, 717, 811, 889, 1059, 1108
BstH2I RGCGCY 1 cut(s) 457
BstMAI GTCTC 3 cut(s) 53, 159, 1866
BstMCI CGRYCG 1 cut(s) 1018
BstNI CCWGG 1 cut(s) 1174
BstPI GGTNACC 1 cut(s) 863
BstSCI CCNGG 1 cut(s) 1172
BstSFI CTRYAG 1 cut(s) 1366
BstSLI GKGCMC 2 cut(s) 529, 1357
BstV1I GCAGC 9 cut(s) 252, 255, 1024, 1027, 1442, 1514, 1853, 1989, 2061
BstX2I RGATCY 2 cut(s) 1153, 1443
BstYI RGATCY 2 cut(s) 1153, 1443
BstZ17I GTATAC 1 cut(s) 1022
BsuRI GGCC 3 cut(s) 929, 1698, 2101
BtgI CCRYGG 1 cut(s) 2078
BtgZI GCGATG 1 cut(s) 1356
BtsCI GGATG 6 cut(s) 708, 717, 811, 889, 1059, 1108
BtsI GCAGTG 1 cut(s) 1292
BtsIMutI CAGTG 2 cut(s) 105, 1292
BveI ACCTGC 1 cut(s) 26
CaiI CAGNNNCTG 1 cut(s) 305
Cfr10I RCCGGY 2 cut(s) 29, 1261
Cfr13I GGNCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
Cfr42I CCGCGG 1 cut(s) 2081
CseI GACGC 6 cut(s) 43, 54, 401, 463, 833, 1938
Csp6I GTAC 6 cut(s) 634, 1132, 1256, 1485, 1528, 1789
CviAII CATG 3 cut(s) 575, 649, 2023
CviQI GTAC 6 cut(s) 634, 1132, 1256, 1485, 1528, 1789
DdeI CTNAG 1 cut(s) 1885
DinI GGCGCC 1 cut(s) 455
DrdI GACNNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 1696
Eam1104I CTCTTC 2 cut(s) 2084, 2090
EarI CTCTTC 2 cut(s) 2084, 2090
Eco24I GRGCYC 1 cut(s) 682
Eco47I GGWCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
Eco57I CTGAAG 1 cut(s) 1953
Eco88I CYCGRG 3 cut(s) 675, 681, 1460
Eco91I GGTNACC 1 cut(s) 863
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 38
EcoO65I GGTNACC 1 cut(s) 863
EcoRII CCWGG 1 cut(s) 1172
EcoT22I ATGCAT 2 cut(s) 1318, 1919
EcoT38I GRGCYC 1 cut(s) 682
EgeI GGCGCC 1 cut(s) 455
EheI GGCGCC 1 cut(s) 455
Esp3I CGTCTC 2 cut(s) 53, 1866
FaeI CATG 3 cut(s) 578, 652, 2026
FaqI GGGAC 2 cut(s) 490, 1097
FatI CATG 3 cut(s) 574, 648, 2022
FauI CCCGC 2 cut(s) 264, 479
FbaI TGATCA 1 cut(s) 567
FblI GTMKAC 2 cut(s) 771, 1021
FokI GGATG 6 cut(s) 715, 724, 798, 896, 1066, 1115
FriOI GRGCYC 1 cut(s) 682
FspBI CTAG 5 cut(s) 911, 1193, 1754, 1779, 2006
FspI TGCGCA 1 cut(s) 428
GsaI CCCAGC 2 cut(s) 302, 2133
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 3 cut(s) 929, 1698, 2101
HapII CCGG 6 cut(s) 30, 451, 716, 1262, 1604, 1976
HgaI GACGC 6 cut(s) 43, 54, 401, 463, 833, 1938
Hin1I GRCGYC 2 cut(s) 54, 454
Hin1II CATG 3 cut(s) 578, 652, 2026
HpaII CCGG 6 cut(s) 30, 451, 716, 1262, 1604, 1976
HphI GGTGA 3 cut(s) 307, 875, 2060
Hpy166II GTNNAC 6 cut(s) 527, 772, 1022, 1114, 1355, 1404
Hpy188I TCNGA 9 cut(s) 189, 218, 422, 696, 737, 894, 1448, 1888, 1954
Hpy188III TCNNGA 8 cut(s) 58, 124, 353, 374, 478, 683, 1268, 1942
Hpy8I GTNNAC 6 cut(s) 527, 772, 1022, 1114, 1355, 1404
Hpy99I CGWCG 4 cut(s) 25, 48, 59, 1954
HpyAV CCTTC 6 cut(s) 223, 505, 959, 1144, 1926, 2023
HpyCH4III ACNGT 4 cut(s) 524, 936, 1352, 1578
HpyCH4IV ACGT 3 cut(s) 1471, 1818, 2057
HpyF3I CTNAG 1 cut(s) 1885
HpySE526I ACGT 3 cut(s) 1471, 1818, 2057
Hsp92I GRCGYC 2 cut(s) 54, 454
Hsp92II CATG 3 cut(s) 578, 652, 2026
KasI GGCGCC 1 cut(s) 453
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 567
KspI CCGCGG 1 cut(s) 2081
LmnI GCTCC 3 cut(s) 541, 592, 1072
Lsp1109I GCAGC 9 cut(s) 252, 255, 1024, 1027, 1442, 1514, 1853, 1989, 2061
LweI GCATC 5 cut(s) 702, 1093, 1159, 1324, 1862
MaeI CTAG 5 cut(s) 911, 1193, 1754, 1779, 2006
MaeII ACGT 3 cut(s) 1471, 1818, 2057
MaeIII GTNAC 5 cut(s) 85, 176, 863, 1835, 2053
MbiI CCGCTC 2 cut(s) 544, 796
MboII GAAGA 9 cut(s) 568, 868, 974, 1208, 1231, 1901, 2101, 2107, 2126
MfeI CAATTG 1 cut(s) 1491
MflI RGATCY 2 cut(s) 1153, 1443
MhlI GDGCHC 3 cut(s) 529, 682, 1357
MluCI AATT 9 cut(s) 113, 163, 515, 956, 1064, 1491, 1609, 1650, 1721
MluI ACGCGT 1 cut(s) 1617
Mly113I GGCGCC 1 cut(s) 454
MlyI GAGTC 4 cut(s) 77, 112, 512, 1472
MmeI TCCRAC 2 cut(s) 1471, 1932
Mph1103I ATGCAT 2 cut(s) 1318, 1919
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 2 cut(s) 690, 1386
MseI TTAA 4 cut(s) 285, 1521, 1821, 1983
MspA1I CMGCKG 1 cut(s) 2080
MspCI CTTAAG 1 cut(s) 1982
MspI CCGG 6 cut(s) 30, 451, 716, 1262, 1604, 1976
MspR9I CCNGG 1 cut(s) 1174
MunI CAATTG 1 cut(s) 1491
Mva1269I GAATGC 5 cut(s) 429, 472, 1844, 1884, 1917
MvaI CCWGG 1 cut(s) 1174
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 454
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 3 cut(s) 578, 652, 2026
NlaIV GGNNCC 3 cut(s) 455, 1155, 1811
NmeAIII GCCGAG 5 cut(s) 30, 35, 40, 639, 1459
NmuCI GTSAC 1 cut(s) 863
NsbI TGCGCA 1 cut(s) 428
NsiI ATGCAT 2 cut(s) 1318, 1919
PaeR7I CTCGAG 1 cut(s) 1460
PauI GCGCGC 2 cut(s) 25, 1924
PcsI WCGNNNNNNNCGW 4 cut(s) 63, 741, 1716, 1845
PctI GAATGC 5 cut(s) 429, 472, 1844, 1884, 1917
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 2 cut(s) 690, 1386
PfeI GAWTC 6 cut(s) 120, 435, 738, 832, 1387, 1423
PflFI GACNNNGTC 1 cut(s) 446
Ple19I CGATCG 1 cut(s) 1018
PleI GAGTC 4 cut(s) 76, 111, 511, 1471
PluTI GGCGCC 1 cut(s) 457
PpsI GAGTC 4 cut(s) 76, 111, 511, 1471
Ppu21I YACGTR 1 cut(s) 1472
PpuMI RGGWCCY 1 cut(s) 38
PsiI TTATAA 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 1172
PspEI GGTNACC 1 cut(s) 863
PspFI CCCAGC 2 cut(s) 298, 2129
PspGI CCWGG 1 cut(s) 1172
PspN4I GGNNCC 3 cut(s) 455, 1155, 1811
PspPI GGNCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
PspPPI RGGWCCY 1 cut(s) 38
PspXI VCTCGAGB 1 cut(s) 1460
PsrI GAACNNNNNNTAC 2 cut(s) 1040, 1072
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 2 cut(s) 1153, 1443
PsyI GACNNNGTC 1 cut(s) 446
PteI GCGCGC 2 cut(s) 25, 1924
PvuI CGATCG 1 cut(s) 1018
RsaI GTAC 6 cut(s) 635, 1133, 1257, 1486, 1529, 1790
RsaNI GTAC 6 cut(s) 634, 1132, 1256, 1485, 1528, 1789
SacII CCGCGG 1 cut(s) 2081
SaqAI TTAA 4 cut(s) 285, 1521, 1821, 1983
Sau96I GGNCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
SchI GAGTC 4 cut(s) 77, 112, 512, 1472
ScrFI CCNGG 1 cut(s) 1174
SduI GDGCHC 3 cut(s) 529, 682, 1357
SfaNI GCATC 5 cut(s) 702, 1093, 1159, 1324, 1862
SfcI CTRYAG 1 cut(s) 1366
SfoI GGCGCC 1 cut(s) 455
Sfr274I CTCGAG 1 cut(s) 1460
Sfr303I CCGCGG 1 cut(s) 2081
SgrAI CRCCGGYG 1 cut(s) 29
SgrBI CCGCGG 1 cut(s) 2081
SinI GGWCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
SlaI CTCGAG 1 cut(s) 1460
SmlI CTYRAG 3 cut(s) 1266, 1460, 1982
SmoI CTYRAG 3 cut(s) 1266, 1460, 1982
Sse9I AATT 9 cut(s) 113, 163, 515, 956, 1064, 1491, 1609, 1650, 1721
SspDI GGCGCC 1 cut(s) 453
SspI AATATT 1 cut(s) 690
SspMI CTAG 5 cut(s) 911, 1193, 1754, 1779, 2006
StyD4I CCNGG 1 cut(s) 1172
TaaI ACNGT 4 cut(s) 524, 936, 1352, 1578
TaiI ACGT 3 cut(s) 1474, 1821, 2060
TasI AATT 9 cut(s) 113, 163, 515, 956, 1064, 1491, 1609, 1650, 1721
TatI WGTACW 1 cut(s) 1527
TauI GCSGC 2 cut(s) 547, 2080
TfiI GAWTC 6 cut(s) 120, 435, 738, 832, 1387, 1423
Tru1I TTAA 4 cut(s) 285, 1521, 1821, 1983
Tru9I TTAA 4 cut(s) 285, 1521, 1821, 1983
TscAI CASTG 2 cut(s) 105, 1292
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 9 cut(s) 265, 268, 1037, 1040, 1430, 1502, 1841, 2002, 2074
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 6 cut(s) 569, 730, 780, 869, 1596, 1698
TspGWI ACGGA 3 cut(s) 356, 1822, 1890
TspRI CASTG 2 cut(s) 105, 1292
Tth111I GACNNNGTC 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 1982
VneI GTGCAC 2 cut(s) 525, 1353
VpaK11BI GGWCC 6 cut(s) 38, 442, 520, 718, 1348, 1809
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 3 cut(s) 956, 1064, 1650
XhoI CTCGAG 1 cut(s) 1460
XmiI GTMKAC 2 cut(s) 771, 1021
XmnI GAANNNNTTC 2 cut(s) 690, 1386
XspI CTAG 5 cut(s) 911, 1193, 1754, 1779, 2006
Zsp2I ATGCAT 2 cut(s) 1318, 1919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.