pycom08g03630

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0019185
Physical Location & Seq
Forward (+)
2825566 .. 2827754
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1599 bp
ATGAAACACGCCATTTACAGAACAATTAAGTGTGAAAGTATGCCAAAGTATCAACAAACCCACAGCAGAAATCATCGAATACTCCTCGCAAAGCCAACCACACACAAAAAAACCCAACAAACCCATTTCATAACGCTTACCCAAAACGCTTACAGTTTCGTACGAGCCAGAACTAAAAATTTCCATCTGGGTACTTTCAGACGAACCCAAAATACCATCCAAAAAATTAAATATATAGAGCAGCACTGTGATAAGCAATTGAAGGAGAAAGGCAGAGAATTTGGACCTTGGGGAGGTCAATATGCTCGTAAAGAGTCCAACCGGACGAACAATCATGATCCTCGCAGCTGTGGTCGTGCAGGCAAGCCATCTCTCTTTCCCTCTGTTACTGAATTTCTCACAAGAAAATTACGAAAGACGAAGGAAGAAGAAATAGCCAGAAATAAGAGCCGAGCCGAGCCGTCGCGTTCTTTCAACTTTAATCAAATTAGAACAATAGTCCATCAACTAAAAATTCATTACCATTGGTTCCTCAAGTCATCAAAATGTGTAGATCTGGTCATTTTCGTCAACTTCATCTGGTCATTTTCGTCAATTTTGTTAGAATTTTATCAAAATGAGTTATGTTGGAAGGATTATTGTTGCAATTGGGGTCCCTCAACTCATCAAAACGTGTGGCTATGGTCATTTTCGTCACATTCGTCACATTTGTATCAAAATGAAACTAATGATAATGAATTTTTAGTTAAGTGTGCACGTTTAAAAAAAAAACGTTACTACAATTTACCCTTACGAAATTGGCACAAAAGTAAAGGGAACAGAGGGGAACTCCTAGTCCAATTTTATCGCGAACTCAGTTTCCTATTTGATTTGGGATTCTCGATTTCCTATTTGATTTGGGACGCTCACACACCTCTCATTCTTTCCTTTCCACACTTTGATTTGGGAATTATGGCGAAGGACAGCAATGGCTACAAGGACACCGATGACAAGCTGCCGCGGGCGAGAAGGGTTAAGAACAAAGCCCCAGCCCCTGTCCAAATCACCGCAGAGCAACTCCTCCGCGAGGTGCGTGAGGGTAATCCAAAAGCTGACGCTTGGATTCGGTATGCCAAGTTTGAGATGAAGAATGGTGACCTTGCAAGGATAAGGGATGTGTTTCAGACGGCAGTGGATATGCTAAACAATGATGAGGGGGCCGAACAGTTGTTTCTAGCTTTTGCTGAATTTGAAGAAGGGTGCAAAGAAATCGAGAGTGCGAGGAGTATTTATAAGTTAGAGAGCGATCGTATACCAAAGGGAAAGGCTGATTCTTTATATAGGATGTTCGAGGAATTTGAGGAGCGGTATGGGGACAGACAAACGATCGAGGATGCAATTTTGAACAAGGCAAGGTTCAAGTGCGAGCAGGAGGTTAGGAAGAATCCTATGGATTATGATGCCTGGTTTGGTTATATAAGGCTAGAAGAAAATGCAGGGAACAAAGAGAAGATTAGACAAGCGACTTACTTTGCTTGTTTTCGTGCATCTATGTTGTTAATTCATAATTGTTTTAGTAGTTTAAGCCATTTTCGTGTGTTTTTAGGTTCATATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf TPR_8

Protein Analysis

533

Amino Acids

62.96

Weight (kDa)

9.49

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAT_Syf1_CNRKL1_C PF23231 365 - 459 3.6e-08 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C PF23241 365 - 433 3.6e-07 PRP39 C-terminal HAT repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51110
malus_domestica MD08G1044800.v1.1 MD08G1045500.v1.1
pyrus_communis pycom08g03610 pycom08g03630 pycom08g03750 pycom08g03760
rosa_samantha Rh5BG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
AccBSI CCGCTC 1 cut(s) 1345
AccI GTMKAC 1 cut(s) 1291
AccII CGCG 4 cut(s) 466, 849, 1000, 1065
AciI CCGC 5 cut(s) 998, 1000, 1047, 1063, 1345
AclI AACGTT 1 cut(s) 772
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 8 cut(s) 178, 278, 392, 513, 605, 737, 1226, 1334
AfaI GTAC 2 cut(s) 162, 193
AfiI CCNNNNNNNGG 2 cut(s) 293, 1066
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 5 cut(s) 262, 475, 1232, 1384, 1399
AjnI CCWGG 1 cut(s) 1442
AloI GAACNNNNNNTCC 4 cut(s) 819, 843, 851, 875
AluBI AGCT 4 cut(s) 348, 994, 1091, 1217
AluI AGCT 4 cut(s) 348, 994, 1091, 1217
Alw21I GWGCWC 1 cut(s) 757
Alw44I GTGCAC 1 cut(s) 753
AlwI GGATC 1 cut(s) 332
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 1 cut(s) 1197
ApaLI GTGCAC 1 cut(s) 753
ApeKI GCWGC 3 cut(s) 241, 345, 994
ApoI RAATTY 8 cut(s) 178, 278, 392, 513, 605, 737, 1226, 1334
AspS9I GGNCC 3 cut(s) 284, 653, 1197
AsuHPI GGTGA 2 cut(s) 1036, 1145
AvaII GGWCC 2 cut(s) 284, 653
BaeGI GKGCMC 1 cut(s) 757
BaeI ACNNNNGTAYC 2 cut(s) 695, 728
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 3 cut(s) 253, 357, 981
BccI CCATC 4 cut(s) 192, 224, 376, 510
BceAI ACGGC 2 cut(s) 445, 1182
BcgI CGANNNNNNTGC 2 cut(s) 1231, 1265
BciT130I CCWGG 1 cut(s) 1444
BfaI CTAG 3 cut(s) 833, 1214, 1463
BglII AGATCT 1 cut(s) 553
BisI GCNGC 4 cut(s) 242, 346, 995, 998
BlsI GCNGC 4 cut(s) 243, 347, 996, 999
Bme1390I CCNGG 1 cut(s) 1444
Bme18I GGWCC 2 cut(s) 284, 653
BmgT120I GGNCC 3 cut(s) 284, 653, 1197
BmiI GGNNCC 4 cut(s) 530, 654, 655, 1198
BmrFI CCNGG 1 cut(s) 1444
BmsI GCATC 3 cut(s) 1363, 1429, 1535
BplI GAGNNNNNCTC 2 cut(s) 813, 845
BpuEI CTTGAG 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 287, 998
BsaWI WCCGGW 1 cut(s) 321
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 1066
Bse3DI GCAATG 1 cut(s) 973
BseBI CCWGG 1 cut(s) 1444
BseDI CCNNGG 2 cut(s) 287, 998
BseGI GGATG 4 cut(s) 216, 1159, 1329, 1378
BseLI CCNNNNNNNGG 2 cut(s) 293, 1066
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 1 cut(s) 868
BseRI GAGGAG 4 cut(s) 74, 1049, 1276, 1355
BseSI GKGCMC 1 cut(s) 757
BseXI GCAGC 3 cut(s) 253, 357, 981
BseYI CCCAGC 1 cut(s) 1027
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 4 cut(s) 466, 849, 1000, 1065
Bsh1285I CGRYCG 2 cut(s) 1288, 1368
BshFI GGCC 1 cut(s) 1199
BsiEI CGRYCG 2 cut(s) 1288, 1368
BsiHKAI GWGCWC 1 cut(s) 757
BsiSI CCGG 1 cut(s) 322
BsiWI CGTACG 1 cut(s) 160
BslFI GGGAC 3 cut(s) 639, 914, 1367
BslI CCNNNNNNNGG 2 cut(s) 293, 1066
BsmFI GGGAC 3 cut(s) 639, 914, 1367
BsnI GGCC 1 cut(s) 1199
Bsp1286I GDGCHC 1 cut(s) 757
Bsp143I GATC 4 cut(s) 337, 553, 1285, 1365
Bsp68I TCGCGA 1 cut(s) 849
BspACI CCGC 5 cut(s) 998, 1000, 1047, 1063, 1345
BspANI GGCC 1 cut(s) 1199
BspCNI CTCAG 1 cut(s) 867
BspFNI CGCG 4 cut(s) 466, 849, 1000, 1065
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 4 cut(s) 530, 654, 655, 1198
BspPI GGATC 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 1345
BsrDI GCAATG 1 cut(s) 973
BssECI CCNNGG 2 cut(s) 287, 998
BssMI GATC 4 cut(s) 337, 553, 1285, 1365
BssNAI GTATAC 1 cut(s) 1292
BssT1I CCWWGG 1 cut(s) 287
Bst1107I GTATAC 1 cut(s) 1292
Bst2UI CCWGG 1 cut(s) 1444
Bst4CI ACNGT 3 cut(s) 155, 248, 1206
BstC8I GCNNGC 4 cut(s) 361, 365, 1002, 1406
BstDEI CTNAG 1 cut(s) 854
BstDSI CCRYGG 1 cut(s) 998
BstEII GGTNACC 1 cut(s) 1133
BstENI CCTNNNNNAGG 2 cut(s) 291, 1064
BstF5I GGATG 4 cut(s) 216, 1159, 1329, 1378
BstFNI CGCG 4 cut(s) 466, 849, 1000, 1065
BstKTI GATC 4 cut(s) 340, 556, 1288, 1368
BstMBI GATC 4 cut(s) 337, 553, 1285, 1365
BstMCI CGRYCG 2 cut(s) 1288, 1368
BstNI CCWGG 1 cut(s) 1444
BstPI GGTNACC 1 cut(s) 1133
BstSCI CCNGG 1 cut(s) 1442
BstSLI GKGCMC 1 cut(s) 757
BstUI CGCG 4 cut(s) 466, 849, 1000, 1065
BstV1I GCAGC 3 cut(s) 253, 357, 981
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BstZ17I GTATAC 1 cut(s) 1292
BsuRI GGCC 1 cut(s) 1199
BtgI CCRYGG 1 cut(s) 998
BtsCI GGATG 4 cut(s) 216, 1159, 1329, 1378
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 2 cut(s) 244, 1176
BtuMI TCGCGA 1 cut(s) 849
Cac8I GCNNGC 4 cut(s) 361, 365, 1002, 1406
CaiI CAGNNNCTG 1 cut(s) 1034
CciI TCATGA 1 cut(s) 334
Cfr13I GGNCC 3 cut(s) 284, 653, 1197
Cfr42I CCGCGG 1 cut(s) 1001
CseI GACGC 2 cut(s) 911, 1103
Csp6I GTAC 2 cut(s) 161, 192
CspCI CAANNNNNGTGG 2 cut(s) 656, 691
CviAII CATG 1 cut(s) 335
CviQI GTAC 2 cut(s) 161, 192
DdeI CTNAG 1 cut(s) 854
DpnI GATC 4 cut(s) 339, 555, 1287, 1367
DpnII GATC 4 cut(s) 337, 553, 1285, 1365
DraI TTTAAA 1 cut(s) 762
Eco130I CCWWGG 1 cut(s) 287
Eco47I GGWCC 2 cut(s) 284, 653
Eco91I GGTNACC 1 cut(s) 1133
EcoNI CCTNNNNNAGG 2 cut(s) 291, 1064
EcoO109I RGGNCCY 1 cut(s) 653
EcoO65I GGTNACC 1 cut(s) 1133
EcoRII CCWGG 1 cut(s) 1442
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 1 cut(s) 338
FaqI GGGAC 3 cut(s) 639, 914, 1367
FatI CATG 1 cut(s) 334
FauI CCCGC 1 cut(s) 993
FauNDI CATATG 1 cut(s) 1591
FblI GTMKAC 1 cut(s) 1291
Fnu4HI GCNGC 4 cut(s) 242, 346, 995, 998
FokI GGATG 4 cut(s) 203, 1166, 1336, 1385
Fsp4HI GCNGC 4 cut(s) 242, 346, 995, 998
FspBI CTAG 3 cut(s) 833, 1214, 1463
GluI GCNGC 4 cut(s) 242, 346, 995, 998
GsaI CCCAGC 1 cut(s) 1031
HaeIII GGCC 1 cut(s) 1199
HapII CCGG 1 cut(s) 322
HgaI GACGC 2 cut(s) 911, 1103
Hin1II CATG 1 cut(s) 338
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 5 cut(s) 314, 876, 1102, 1310, 1423
HpaII CCGG 1 cut(s) 322
HphI GGTGA 2 cut(s) 1036, 1145
Hpy166II GTNNAC 3 cut(s) 571, 755, 1292
Hpy188I TCNGA 2 cut(s) 200, 1164
Hpy188III TCNNGA 4 cut(s) 335, 848, 880, 1252
Hpy8I GTNNAC 3 cut(s) 571, 755, 1292
Hpy99I CGWCG 1 cut(s) 466
HpyAV CCTTC 6 cut(s) 256, 415, 625, 952, 1002, 1229
HpyCH4III ACNGT 3 cut(s) 155, 248, 1206
HpyCH4IV ACGT 3 cut(s) 672, 757, 772
HpyCH4V TGCA 8 cut(s) 359, 645, 755, 1142, 1242, 1376, 1475, 1526
HpyF3I CTNAG 1 cut(s) 854
HpySE526I ACGT 3 cut(s) 672, 757, 772
Hsp92II CATG 1 cut(s) 338
KflI GGGWCCC 1 cut(s) 653
KspI CCGCGG 1 cut(s) 1001
Kzo9I GATC 4 cut(s) 337, 553, 1285, 1365
LmnI GCTCC 1 cut(s) 1342
Lsp1109I GCAGC 3 cut(s) 253, 357, 981
LweI GCATC 3 cut(s) 1363, 1429, 1535
MaeI CTAG 3 cut(s) 833, 1214, 1463
MaeII ACGT 3 cut(s) 672, 757, 772
MaeIII GTNAC 5 cut(s) 385, 693, 702, 773, 1133
MalI GATC 4 cut(s) 339, 555, 1287, 1367
MbiI CCGCTC 1 cut(s) 1345
MboI GATC 4 cut(s) 337, 553, 1285, 1365
MboII GAAGA 7 cut(s) 437, 440, 1138, 1244, 1432, 1478, 1501
MfeI CAATTG 2 cut(s) 257, 646
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 757
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 342, 608
MseI TTAA 8 cut(s) 27, 228, 480, 747, 761, 1014, 1538, 1562
MslI CAYNNNNRTG 2 cut(s) 544, 1572
MspA1I CMGCKG 2 cut(s) 348, 1000
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 1 cut(s) 1444
MunI CAATTG 2 cut(s) 257, 646
MvaI CCWGG 1 cut(s) 1444
MvnI CGCG 4 cut(s) 466, 849, 1000, 1065
NdeI CATATG 1 cut(s) 1591
NdeII GATC 4 cut(s) 337, 553, 1285, 1365
NlaIII CATG 1 cut(s) 338
NlaIV GGNNCC 4 cut(s) 530, 654, 655, 1198
NmeAIII GCCGAG 2 cut(s) 476, 481
NmuCI GTSAC 3 cut(s) 693, 702, 1133
NruI TCGCGA 1 cut(s) 849
PagI TCATGA 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PfeI GAWTC 4 cut(s) 876, 1102, 1310, 1423
Pfl23II CGTACG 1 cut(s) 160
PkrI GCNGC 4 cut(s) 243, 347, 996, 999
Ple19I CGATCG 2 cut(s) 1288, 1368
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 653
PsiI TTATAA 1 cut(s) 1272
Psp1406I AACGTT 1 cut(s) 772
Psp5II RGGWCCY 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 1442
PspEI GGTNACC 1 cut(s) 1133
PspFI CCCAGC 1 cut(s) 1027
PspGI CCWGG 1 cut(s) 1442
PspLI CGTACG 1 cut(s) 160
PspN4I GGNNCC 4 cut(s) 530, 654, 655, 1198
PspPI GGNCC 3 cut(s) 284, 653, 1197
PspPPI RGGWCCY 1 cut(s) 653
PsrI GAACNNNNNNTAC 2 cut(s) 196, 228
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 553
PvuI CGATCG 2 cut(s) 1288, 1368
PvuII CAGCTG 1 cut(s) 348
RruI TCGCGA 1 cut(s) 849
RsaI GTAC 2 cut(s) 162, 193
RsaNI GTAC 2 cut(s) 161, 192
RseI CAYNNNNRTG 2 cut(s) 544, 1572
SacII CCGCGG 1 cut(s) 1001
SaqAI TTAA 8 cut(s) 27, 228, 480, 747, 761, 1014, 1538, 1562
SatI GCNGC 4 cut(s) 242, 346, 995, 998
Sau3AI GATC 4 cut(s) 337, 553, 1285, 1365
Sau96I GGNCC 3 cut(s) 284, 653, 1197
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 1444
SduI GDGCHC 1 cut(s) 757
SfaNI GCATC 3 cut(s) 1363, 1429, 1535
Sfr303I CCGCGG 1 cut(s) 1001
SgrBI CCGCGG 1 cut(s) 1001
SinI GGWCC 2 cut(s) 284, 653
SmiMI CAYNNNNRTG 2 cut(s) 544, 1572
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
SsiI CCGC 5 cut(s) 998, 1000, 1047, 1063, 1345
SspMI CTAG 3 cut(s) 833, 1214, 1463
StyD4I CCNGG 1 cut(s) 1442
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 3 cut(s) 155, 248, 1206
TaiI ACGT 3 cut(s) 675, 760, 775
TaqI TCGA 5 cut(s) 76, 881, 1251, 1329, 1368
TauI GCSGC 1 cut(s) 1000
TfiI GAWTC 4 cut(s) 876, 1102, 1310, 1423
Tru1I TTAA 8 cut(s) 27, 228, 480, 747, 761, 1014, 1538, 1562
Tru9I TTAA 8 cut(s) 27, 228, 480, 747, 761, 1014, 1538, 1562
TscAI CASTG 2 cut(s) 251, 1176
TseFI GTSAC 3 cut(s) 693, 702, 1133
TseI GCWGC 3 cut(s) 241, 345, 994
Tsp45I GTSAC 3 cut(s) 693, 702, 1133
TspDTI ATGAA 9 cut(s) 17, 118, 506, 565, 735, 750, 1139, 1532, 1578
TspRI CASTG 2 cut(s) 251, 1176
VneI GTGCAC 1 cut(s) 753
VpaK11BI GGWCC 2 cut(s) 284, 653
XagI CCTNNNNNAGG 2 cut(s) 291, 1064
XapI RAATTY 8 cut(s) 178, 278, 392, 513, 605, 737, 1226, 1334
XmiI GTMKAC 1 cut(s) 1291
XspI CTAG 3 cut(s) 833, 1214, 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.