pycom08g03660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0024656
Physical Location & Seq
Forward (+)
2833383 .. 2834762
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03660.3

Sequence Viewer

Length: 1008 bp
ATGGTAGAACTCATATCCCATAGTTTCTCTAGGATATCCCACAAAATACTTAATGTGATTAAGACTTGGCTTTCTACCATGCCATATCTCATAGGGCCTGCTGTATATAGAGCATATCCCCAGAATGTTACTGGCAGATCAGCGGAACTCATCATAGAACGAACCATGTTCATCAAGGTTCGATTTCTCCTTTCAGAAACTCTATTAAGTTGTGGAGTTCCCGCTAGGAATTCATTACTCAGATATTCACCCCCTCGATCTGATCTTAGGATTTTTATCTGTTTCCCAGTTTGCTTCTCAACTTCATTCTTGAATTCTTTGAACCTTTCAAAGGATTCTGACTTAGGTCCACATACGTCAGTGTGGATTAACCCTAGAATTTCAGTGGCACGCTCTCCTTTCCCAGTGAATGGAGATTTGGTCATCTTCCCTTTCAAACAGCATTCACAAGTACCCATCTGGTCATTACCTAATGGGCGGACATATCCGTCTTTCGACATCTTGCGAATCTTTTCAAGGTTCACATGTCCAAGTTTAAGATGCCACATCCTTTCTTGGTTAACTTCTTCTCTAGCCCTTTTGGGTTTTGAGGTATTCCCGCTTTCAATACAGTGCATCCCACTATTCATAATACGACCCTTCAAGTATAAAGTGCAACTCATTTTGTCAAACAATAATGAGTGCCCATCTCGTAAAAGCTTAGAGATAGAAATCAAATTCTTTATACATGAAGGAAAATATAAACAATTTTTAAGTTCCAGGACTTCCCCAGAGGCGTCGTTCCCAACTCGCACAACCATCTCCCCATTGCGTAGTGTCTTGCTCCCTACTAGTCCCTATCCAGGAATTGGAGCTCACTGTAAAAGCACTTTCTATGACCGAAATAGTCTCTTCAAAAGTTCGTGTCATAAGGCTCGCAATGCGCACCCTGTATTTCTTCTTCCAACGTTCATCCTTTCCACAATGAAAGCATGTTCCTTTGGATTTCTTTGCCTTCTTCTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

336

Amino Acids

38.11

Weight (kDa)

9.9

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024656)

Species Orthologous Gene IDs
pyrus_communis pycom08g03660 pycom17g16770
rosa_chinensis RchiOBHm_Chr2g0086181

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 487
Acc16I TGCGCA 1 cut(s) 924
AccB7I CCANNNNNTGG 2 cut(s) 410, 848
AciI CCGC 4 cut(s) 143, 222, 478, 599
AclI AACGTT 1 cut(s) 947
AcsI RAATTY 4 cut(s) 229, 313, 378, 716
AcyI GRCGYC 1 cut(s) 776
AfaI GTAC 1 cut(s) 453
AfiI CCNNNNNNNGG 4 cut(s) 331, 410, 842, 848
AflII CTTAAG 1 cut(s) 1001
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 8 cut(s) 313, 322, 330, 436, 516, 606, 643, 895
AhlI ACTAGT 1 cut(s) 830
AjnI CCWGG 2 cut(s) 758, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 2 cut(s) 699, 854
AluI AGCT 2 cut(s) 699, 854
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 1 cut(s) 893
AoxI GGCC 1 cut(s) 95
ApoI RAATTY 4 cut(s) 229, 313, 378, 716
Asp700I GAANNNNTTC 1 cut(s) 511
AspLEI GCGC 1 cut(s) 925
AspS9I GGNCC 2 cut(s) 95, 347
AsuHPI GGTGA 1 cut(s) 240
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 686
BanII GRGCYC 1 cut(s) 856
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
Bbv12I GWGCWC 1 cut(s) 856
BccI CCATC 3 cut(s) 464, 694, 806
BciT130I CCWGG 2 cut(s) 760, 843
BcoDI GTCTC 1 cut(s) 893
BcuI ACTAGT 1 cut(s) 830
BfaI CTAG 5 cut(s) 30, 225, 375, 572, 831
BfrI CTTAAG 1 cut(s) 1001
Bme1390I CCNGG 2 cut(s) 760, 843
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 95, 347
BmrFI CCNGG 2 cut(s) 760, 843
BmrI ACTGGG 2 cut(s) 281, 398
BmsI GCATC 2 cut(s) 530, 624
BmuI ACTGGG 2 cut(s) 281, 398
BoxI GACNNNNGTC 1 cut(s) 345
BsaBI GATNNNNATC 2 cut(s) 275, 710
BsaHI GRCGYC 1 cut(s) 776
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 410, 842, 848
Bse1I ACTGG 3 cut(s) 136, 287, 404
Bse3DI GCAATG 2 cut(s) 806, 925
Bse8I GATNNNNATC 2 cut(s) 275, 710
BseBI CCWGG 2 cut(s) 760, 843
BseGI GGATG 3 cut(s) 546, 615, 951
BseJI GATNNNNATC 2 cut(s) 275, 710
BseLI CCNNNNNNNGG 4 cut(s) 331, 410, 842, 848
BseMI GCAATG 2 cut(s) 806, 925
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 3 cut(s) 136, 287, 404
BseSI GKGCMC 1 cut(s) 686
BshFI GGCC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 856
BslFI GGGAC 1 cut(s) 819
BslI CCNNNNNNNGG 4 cut(s) 331, 410, 842, 848
BsmAI GTCTC 1 cut(s) 893
BsmFI GGGAC 1 cut(s) 819
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 686, 856
Bsp143I GATC 3 cut(s) 137, 257, 262
BspACI CCGC 4 cut(s) 143, 222, 478, 599
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 1001
BsrDI GCAATG 2 cut(s) 806, 925
BsrI ACTGG 3 cut(s) 136, 287, 404
BssMI GATC 3 cut(s) 137, 257, 262
BssNI GRCGYC 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 760, 843
Bst4CI ACNGT 2 cut(s) 612, 860
Bst6I CTCTTC 1 cut(s) 896
BstACI GRCGYC 1 cut(s) 776
BstAFI CTTAAG 1 cut(s) 1001
BstC8I GCNNGC 3 cut(s) 99, 391, 916
BstDEI CTNAG 4 cut(s) 239, 266, 343, 700
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 3 cut(s) 546, 615, 951
BstHHI GCGC 1 cut(s) 925
BstKTI GATC 3 cut(s) 140, 260, 265
BstMAI GTCTC 1 cut(s) 893
BstMBI GATC 3 cut(s) 137, 257, 262
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 2 cut(s) 760, 843
BstNSI RCATGY 2 cut(s) 528, 975
BstPAI GACNNNNGTC 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 758, 841
BstSLI GKGCMC 1 cut(s) 686
BsuRI GGCC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 546, 615, 951
BtsIMutI CAGTG 5 cut(s) 366, 390, 411, 617, 856
Cac8I GCNNGC 3 cut(s) 99, 391, 916
CfoI GCGC 1 cut(s) 925
Cfr13I GGNCC 2 cut(s) 95, 347
CseI GACGC 1 cut(s) 765
Csp6I GTAC 1 cut(s) 452
CviAII CATG 5 cut(s) 79, 166, 525, 728, 972
CviJI RGCY 6 cut(s) 70, 97, 575, 699, 854, 914
CviKI_1 RGCY 6 cut(s) 70, 97, 575, 699, 854, 914
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 4 cut(s) 239, 266, 343, 700
DpnI GATC 3 cut(s) 139, 259, 264
DpnII GATC 3 cut(s) 137, 257, 262
DrdI GACNNNNNNGTC 1 cut(s) 487
DseDI GACNNNNNNGTC 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
EciI GGCGGA 1 cut(s) 493
Ecl136II GAGCTC 1 cut(s) 854
Eco24I GRGCYC 1 cut(s) 856
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 854
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 2 cut(s) 229, 313
EcoRII CCWGG 2 cut(s) 758, 841
EcoRV GATATC 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 856
FaeI CATG 5 cut(s) 82, 169, 528, 731, 975
FaqI GGGAC 1 cut(s) 819
FatI CATG 5 cut(s) 78, 165, 524, 727, 971
FauI CCCGC 2 cut(s) 229, 606
FokI GGATG 3 cut(s) 533, 602, 938
FriOI GRGCYC 1 cut(s) 856
FspAI RTGCGCAY 1 cut(s) 924
FspBI CTAG 5 cut(s) 30, 225, 375, 572, 831
FspI TGCGCA 1 cut(s) 924
GlaI GCGC 1 cut(s) 924
HaeIII GGCC 1 cut(s) 97
HgaI GACGC 1 cut(s) 765
HhaI GCGC 1 cut(s) 925
Hin1I GRCGYC 1 cut(s) 776
Hin1II CATG 5 cut(s) 82, 169, 528, 731, 975
Hin6I GCGC 1 cut(s) 923
HinP1I GCGC 1 cut(s) 923
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HindIII AAGCTT 1 cut(s) 697
HinfI GANTC 2 cut(s) 335, 507
HpaI GTTAAC 1 cut(s) 561
HphI GGTGA 1 cut(s) 240
Hpy166II GTNNAC 3 cut(s) 350, 522, 561
Hpy188I TCNGA 4 cut(s) 196, 242, 262, 340
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 3 cut(s) 350, 522, 561
Hpy99I CGWCG 1 cut(s) 781
HpyAV CCTTC 3 cut(s) 649, 725, 1004
HpyCH4III ACNGT 2 cut(s) 612, 860
HpyCH4IV ACGT 2 cut(s) 356, 947
HpyCH4V TGCA 2 cut(s) 615, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 4 cut(s) 239, 266, 343, 700
HpySE526I ACGT 2 cut(s) 356, 947
Hsp92I GRCGYC 1 cut(s) 776
Hsp92II CATG 5 cut(s) 82, 169, 528, 731, 975
HspAI GCGC 1 cut(s) 923
KspAI GTTAAC 1 cut(s) 561
Kzo9I GATC 3 cut(s) 137, 257, 262
LmnI GCTCC 2 cut(s) 828, 851
LweI GCATC 2 cut(s) 530, 624
MaeI CTAG 5 cut(s) 30, 225, 375, 572, 831
MaeII ACGT 2 cut(s) 356, 947
MaeIII GTNAC 1 cut(s) 127
MalI GATC 3 cut(s) 139, 259, 264
MboI GATC 3 cut(s) 137, 257, 262
MboII GAAGA 6 cut(s) 418, 558, 883, 929, 932, 989
MhlI GDGCHC 2 cut(s) 686, 856
MluCI AATT 6 cut(s) 229, 313, 378, 716, 746, 846
MmeI TCCRAC 1 cut(s) 968
MnlI CCTC 3 cut(s) 264, 583, 766
MroXI GAANNNNTTC 1 cut(s) 511
MseI TTAA 8 cut(s) 51, 60, 206, 369, 536, 560, 752, 1002
MspA1I CMGCKG 1 cut(s) 143
MspCI CTTAAG 1 cut(s) 1001
MspR9I CCNGG 2 cut(s) 760, 843
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 2 cut(s) 760, 843
MwoI GCNNNNNNNGC 1 cut(s) 920
NdeII GATC 3 cut(s) 137, 257, 262
NlaIII CATG 5 cut(s) 82, 169, 528, 731, 975
NsbI TGCGCA 1 cut(s) 924
NspI RCATGY 2 cut(s) 528, 975
PciI ACATGT 1 cut(s) 524
PctI GAATGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 2 cut(s) 335, 507
PflMI CCANNNNNTGG 2 cut(s) 410, 848
PfoI TCCNGGA 2 cut(s) 758, 841
PscI ACATGT 1 cut(s) 524
PshAI GACNNNNGTC 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 856
Psp1406I AACGTT 1 cut(s) 947
Psp6I CCWGG 2 cut(s) 758, 841
PspGI CCWGG 2 cut(s) 758, 841
PspPI GGNCC 2 cut(s) 95, 347
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
SacI GAGCTC 1 cut(s) 856
SaqAI TTAA 8 cut(s) 51, 60, 206, 369, 536, 560, 752, 1002
Sau3AI GATC 3 cut(s) 137, 257, 262
Sau96I GGNCC 2 cut(s) 95, 347
ScrFI CCNGG 2 cut(s) 760, 843
SduI GDGCHC 2 cut(s) 686, 856
SfaNI GCATC 2 cut(s) 530, 624
SinI GGWCC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
SpeI ACTAGT 1 cut(s) 830
Sse9I AATT 6 cut(s) 229, 313, 378, 716, 746, 846
SsiI CCGC 4 cut(s) 143, 222, 478, 599
SspMI CTAG 5 cut(s) 30, 225, 375, 572, 831
SstI GAGCTC 1 cut(s) 856
StyD4I CCNGG 2 cut(s) 758, 841
TaaI ACNGT 2 cut(s) 612, 860
TaiI ACGT 2 cut(s) 359, 950
TaqI TCGA 3 cut(s) 181, 256, 495
TaqII GACCGA 1 cut(s) 894
TasI AATT 6 cut(s) 229, 313, 378, 716, 746, 846
TfiI GAWTC 2 cut(s) 335, 507
Tru1I TTAA 8 cut(s) 51, 60, 206, 369, 536, 560, 752, 1002
Tru9I TTAA 8 cut(s) 51, 60, 206, 369, 536, 560, 752, 1002
TscAI CASTG 5 cut(s) 366, 390, 411, 617, 863
TspDTI ATGAA 7 cut(s) 160, 222, 294, 616, 744, 940, 980
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 5 cut(s) 366, 390, 411, 617, 863
Van91I CCANNNNNTGG 2 cut(s) 410, 848
Vha464I CTTAAG 1 cut(s) 1001
VpaK11BI GGWCC 1 cut(s) 347
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 4 cut(s) 229, 313, 378, 716
XceI RCATGY 2 cut(s) 528, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 511
XspI CTAG 5 cut(s) 30, 225, 375, 572, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.